>AFF475 (AFF475Q) /CSM/AF/AFF4-D/AFF475Q.Seq.d/
XXXXXXXXXXGAAGGTGCACTCTCACTCAAAGAGATTAAACAATTCATTCTTGATGAATG
TGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTACAAAAGATCTTCAAACTCAT
CCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCTCTGATACCATTCGTCCAAT
CTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCAATGATGGTAGTAAACTCAC
TCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAGAACAAAAGAATAAAAAACT
CATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTATTTTCGTCAAATCTGTTAG
ACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTTTCCCATCCATCTGTATTCA
TCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTAAATTCAAAAACTTTGAATC
TCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCGATATTGAACGTGTCAATGT
CGTCATCAACTACGATATGGCTGAATCTG

---GALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLSDTIRPIC
KKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVIFVKSVRR
ADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGIDIERVNVV
INYDMAES

Frame A:
---kvhshskrlnnsflmnvipcsnhwi*ermykrssnssqptnkl*cfqlpslipfvqs
vrsl*tihwkstsmmvvnslsmvfnnttyqslknkriknslnswih*tlikplfssnlld
vlmp*irsskilvshpsvfivtsikrivsnntvnsktlnlvswlplislvvvsilnvsms
ssttiwlnl


Frame B:
---rctltqrd*tihs**m*yhariigyekgctkdlqthpsqqtsydvfsypl*yhssnl
*evyeqsignlhq*w**thspwsstilrtnh*rtke*kth*tlgfirl*ssryfrqic*t
c*clk*dpprywfpihlyss*prskgsyrtip*iqkl*isyhgch*ylwswyry*tcqcr
hqlryg*i


Frame C:
---GALSLKEIKQFILDECDTMLESLDMRKDVQKIFKLIPANKQVMMFSATLSDTIRPIC
KKFMNNPLEIYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVIFVKSVRR
ADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGIDIERVNVV
INYDMAES

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFE359 (VFE359Q) /CSM/VF/VFE3-C/VFE359Q.Seq.d/                       1108   0.0
VFD348 (VFD348Q) /CSM/VF/VFD3-B/VFD348Q.Seq.d/                       1108   0.0
VFA442 (VFA442Q) /CSM/VF/VFA4-B/VFA442Q.Seq.d/                       1108   0.0
SFF569 (SFF569Q) /CSM/SF/SFF5-C/SFF569Q.Seq.d/                       1108   0.0
FC-AI06 (FC-AI06Q) /CSM/FC/FC-AI/FC-AI06Q.Seq.d/                     1108   0.0
AFO646 (AFO646Q) /CSM/AF/AFO6-B/AFO646Q.Seq.d/                       1108   0.0
AFO484 (AFO484Q) /CSM/AF/AFO4-D/AFO484Q.Seq.d/                       1108   0.0
AFN644 (AFN644Q) /CSM/AF/AFN6-B/AFN644Q.Seq.d/                       1108   0.0
AFM853 (AFM853Q) /CSM/AF/AFM8-C/AFM853Q.Seq.d/                       1108   0.0
AFM640 (AFM640Q) /CSM/AF/AFM6-B/AFM640Q.Seq.d/                       1108   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AW759959|AW759959.1 sl56c07.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-5317 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56   8e-10  2
BF071471|BF071471.1 st59b09.y1 Gm-c1053 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1053-857 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    56   8e-10  2
AL408654|AL408654.1 T7 end of clone AV0AA009H09 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    38   1e-09  4
AX488925|AX488925.1 Sequence 6225 from Patent WO02053728.          40   2e-09  5
CA251168|CA251168.1 SCQSFL1122D07.g FL1 Saccharum officinarum cDNA clone SCQSFL1122D07 5', mRNA sequence.    48   8e-09  3
CA169742|CA169742.1 SCQGSB1066A04.g SB1 Saccharum officinarum cDNA clone SCQGSB1066A04 5', mRNA sequence.    48   1e-08  3
CA201831|CA201831.1 SCRLFL1008H03.g FL1 Saccharum officinarum cDNA clone SCRLFL1008H03 5', mRNA sequence.    48   1e-08  3
BI974435|BI974435.1 saj01e10.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-9068 5' similar to TR:Q9ZS06 Q9ZS06 RNA HELICASE ;, mRNA sequence.    52   1e-08  2
CA252463|CA252463.1 SCVPFL1138E12.g FL1 Saccharum officinarum cDNA clone SCVPFL1138E12 5', mRNA sequence.    48   1e-08  3
BI742220|BI742220.1 kt91h02.y1 Strongyloides ratti L3 adult pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:HE47_CAEEL Q18212 PROBABLE ATP-DEPENDENT RNA HELICASE P47 HOMOLOG. [1] ;, mRNA sequence.    38   1e-07  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O13792) RecName: Full=ATP-dependent RNA helicase uap56;        ...   254   1e-66
D89270_1(D89270|pid:none) Schizosaccharomyces pombe mRNA, partia...   254   1e-66
(A1DL85) RecName: Full=ATP-dependent RNA helicase sub2;         ...   252   4e-66
AJ010466_1(AJ010466|pid:none) Arabidopsis thaliana mRNA for DEAD...   252   4e-66
(Q1DI07) RecName: Full=ATP-dependent RNA helicase SUB2;         ...   252   5e-66
AM920437_2400(AM920437|pid:none) Penicillium chrysogenum Wiscons...   251   7e-66
(A1CMQ7) RecName: Full=ATP-dependent RNA helicase sub2;         ...   251   7e-66
AJ428513_1(AJ428513|pid:none) Chironomus tentans mRNA for HEL pr...   251   1e-65
GM694469_1(GM694469|pid:none) Sequence 25 from Patent EP1953235.      251   1e-65
GM694505_1(GM694505|pid:none) Sequence 61 from Patent EP1953235....   250   2e-65

 psg: 0.56  gvh: 0.46  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFF475 is cyt

>AFF475Z.Seq
----------GAAGGTGCACTCTCACTCAAAGAGATTAAACAATTCATTCTTGATGAATG
TGATACCATGCTCGAATCATTGGATATGAGAAAGGATGTACAAAAGATCTTCAAACTCAT
CCCAGCCAACAAACAAGTTATGATGTTTTCAGCTACCCTCTCTGATACCATTCGTCCAAT
CTGTAAGAAGTTTATGAACAATCCATTGGAAATCTACATCAATGATGGTAGTAAACTCAC
TCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAAGAACAAAAGAATAAAAAACT
CATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTTATTTTCGTCAAATCTGTTAG
ACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGTTTCCCATCCATCTGTATTCA
TCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGTAAATTCAAAAACTTTGAATC
TCGTATCATGGTTGCCACTAATATCTTTGGTCGTGGTATCGATATTGAACGTGTCAATGT
CGTCATCAACTACGATATGGCTGAATCTG