>AFF459 (AFF459Q) /CSM/AF/AFF4-C/AFF459Q.Seq.d/
AAAATGGATTCAAATAAAGTTATCGTTATGGATAATGGTACTGGTTTTGTAAAATGTGGT
TTTGCAGGTGCAAATTTCCCAACAGCCATTTTTCCATCAATGGTTGGTAGACCAATTTTA
CGTTCAGAAGAAAAGGTAGAAAATGTAGAGATTAAAGATATTATGGGTGGTGATGAAGCA
NCCAAATTAAGATCAATGTTACAAATCACCTATCCATTGGAAAATGGTATXXXXXXXXXX

KMDSNKVIVMDNGTGFVKCGFAGANFPTAIFPSMVGRPILRSEEKVENVEIKDIMGGDEA
XKLRSMLQITYPLENG---

Frame A:
KMDSNKVIVMDNGTGFVKCGFAGANFPTAIFPSMVGRPILRSEEKVENVEIKDIMGGDEA
XKLRSMLQITYPLENG---


Frame B:
kwiqiklslwimvlvl*nvvlqvqisqqpffhqwlvdqfyvqkkr*km*rlkilwvvmkx
pn*dqcykspihwkmv---


Frame C:
ngfk*syryg*wywfckmwfcrckfpnshfsingw*tnftfrrkgrkcrd*ryygw**sx
qikinvtnhlsigkw---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF459 (AFF459Q) /CSM/AF/AFF4-C/AFF459Q.Seq.d/                        450   e-126
VFI201 (VFI201Q) /CSM/VF/VFI2-A/VFI201Q.Seq.d/                        442   e-123
FC-AH01 (FC-AH01Q) /CSM/FC/FC-AH/FC-AH01Q.Seq.d/                      442   e-123
FC-131 (FC-131Q) /CSM/CG/CG-FC/FC-131Q.Seq.d/                         442   e-123
AFF191 (AFF191Q) /CSM/AF/AFF1-D/AFF191Q.Seq.d/                        438   e-122
SSL386 (SSL386Q) /CSM/SS/SSL3-D/SSL386Q.Seq.d/                        123   1e-27
VHK249 (VHK249Q) /CSM/VH/VHK2-C/VHK249Q.Seq.d/                         34   0.90
SHJ288 (SHJ288Q) /CSM/SH/SHJ2-D/SHJ288Q.Seq.d/                         34   0.90
FC-BN06 (FC-BN06Q) /CSM/FC/FC-BN/FC-BN06Q.Seq.d/                       34   0.90
VSG104 (VSG104Q) /CSM/VS/VSG1-A/VSG104Q.Seq.d/                         32   3.5

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF095929|AF095929.1 Dictyostelium discoideum actin related protein 2 (arpB) mRNA, complete cds.   442   e-120  1
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   353   e-109  4
AZ542124|AZ542124.1 ENTDP34TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    84   1e-12  1
AZ673974|AZ673974.1 ENTMJ67TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    82   4e-12  1
BP185878|BP185878.1 Dugesia japonica cDNA, clone: 02704_HH, expressed in the head.    38   2e-04  2
BM879767|BM879767.1 kt99f09.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:ARP2_CAEEL P53489 ACTIN-LIKE PROTEIN 2 ;, mRNA sequence.    44   4e-04  2
BM879747|BM879747.1 kt99d10.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:ARP2_CAEEL P53489 ACTIN-LIKE PROTEIN 2 ;, mRNA sequence.    44   6e-04  2
CD524205|CD524205.1 ku97c07.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:ARP2_CAEEL P53489 ACTIN-LIKE PROTEIN 2 ;, mRNA sequence.    44   6e-04  2
CD524715|CD524715.1 kw10d08.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:ARP2_CAEEL P53489 ACTIN-LIKE PROTEIN 2 ;, mRNA sequence.    44   6e-04  2
CD420965|CD420965.1 ku93f10.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:ARP2_CAEEL P53489 ACTIN-LIKE PROTEIN 2 ;, mRNA sequence.    44   6e-04  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96621) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   147   1e-34
(P53487) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   129   2e-29
BC085445_1(BC085445|pid:none) Danio rerio zgc:101810, mRNA (cDNA...   114   1e-24
(Q7SXW6) RecName: Full=Actin-related protein 2-A; AltName: Full=...   113   2e-24
(Q5R4K0) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   113   2e-24
AY891099_1(AY891099|pid:none) Synthetic construct Homo sapiens c...   113   2e-24
AK033014_1(AK033014|pid:none) Mus musculus 12 days embryo female...   113   2e-24
BT058845_1(BT058845|pid:none) Salmo salar clone ssal-rgf-507-102...   113   2e-24
(Q5M7U6) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   113   2e-24
(Q5BL41) RecName: Full=Actin-related protein 2; AltName: Full=Ac...   112   3e-24

 psg: 0.73  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFF459 is cyt

>AFF459F.Seq
AAAATGGATTCAAATAAAGTTATCGTTATGGATAATGGTACTGGTTTTGTAAAATGTGGT
TTTGCAGGTGCAAATTTCCCAACAGCCATTTTTCCATCAATGGTTGGTAGACCAATTTTA
CGTTCAGAAGAAAAGGTAGAAAATGTAGAGATTAAAGATATTATGGGTGGTGATGAAGCA
NCCAAATTAAGATCAATGTTACAAATCACCTATCCATTGGAAAATGGTAT----------