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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF455
Library AF
(Link to library)
Clone ID AFF455
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16477-1
Original site URL
Representative seq. ID AFF455P
(Link to Original site)
Representative DNA sequence 
>AFF455 (AFF455Q) /CSM/AF/AFF4-C/AFF455Q.Seq.d/
TAATAATACCAACAACACTAACAACATTGAAATGGATGATAATAAAAGTATTAATAACAA
CAACAACAACATTAACAGTATAAATAGTATGTATAGAGGCACTAGTAATTTACTCAATCC
ATTACCTTTTCATCATACTAATCAACAACGTAATGGTGGGGGTTCTAATAATCATCAAAT
CAATAATCACCATAAAGATGAAGATGAAGAGATTGATGAGGACGAGGAGAAATTCATTCA
CAAGAATGAAAATTTTACAGATTTTATTCCAAGACTTTCAAAGGATTGGTTCGCAGAAAG
ATCTAAATATATTCCAATTCGTTTAAATATTCGTGAGAGAAATGATCTTCGTCTTTTAGA
GGCAGCACTTCACGTTTCAGAATATACAGATAAAATCGATATCATTCATGTTGGTGGTTC
AAAATCAAAAAGAATAAATGATCAATTAAGAGGTATGTGTGCAATTTTATCAGGTTTATT
GGTTGCAAGTGATTTTAAAAAAGGTCAACAATTAGTTGAAAATAAAGATTTCTTTGAAAA
TGAAGAATTCTTTCAAAATGTTTTTGAAATTGGTAGAAGACACAAGATTATGAATCCAGC
AAAGATGAGAACTGAATATGGTAAAATGATTCATTTATTACAAGATGCAGCAGATGAAGA
TGTAAAGAAAAATCTTAGTGGACTTAATATGATTAAACCATTGAAAACCGTTTTCTTGTT
TTTGGATGAACGTAACGGACTCAAATTGTTAGAGGATCCATTGTTAGAATTGGCAACTCG
TGAGGTTTTAGCAGAGAATAAAACCAGACCTCAAATTCAACGTGAAATTCGTGAAAAGGA
ACATGCAATCAAAACATTATCACGTCGTTACTCATCATCAATGTTGAAATCAGAGGGAGG
XXXXXXXXXXCTCAAGGAATACTTTAGTCCAAATCAATCAGAGAGAGGTTTTTCATTGGC
TTTGGAACATGGCATTGGTGGTGCACGTCTTTCTCACAATCATCGTAAACAATATAACTA
TGTATATCAATCACTCTCCTTGTGGCAACATATTCTTCATGATATGTTTAAACTTTGGTA
CTTTGCAGAAATTGATCTTTTATGCGGACACAGATATCAATTATCAAACACTGGTCAAGG
TCTAAATCGTATTCAACAATGTCCAAACGTTGGTAAAATTATGCATAGTATTCTACGTCA
AACTCAAAAAAAAGTTGGTGATGATTGGGTTGGTTCTTCCGTTATTCATTTGGGTGATCA
TAACGTTCCAAACTCTTTGGTTTTCATCGATAAATACACTCAAATCTCTAGAATCCTAAG
TCCAATCGTTTTAACCATTGAATATATTCCAAAGATCACCGATCCAAATTTAGTTGCTTA
CATCAAAAACACCTTTGGTGGTCCACAAACTTTAACCAAAATCATCCTTTGTGATTTCTT
TAAACACGCTTTCGATGGTAGTGGTGCCGATAACTTTTTCGATGCTGGCTCTTGTATTGA
TGGTAGATTAACTTCTGCTTGGCAATGGTCTGCTCAAATCGCAAAGAAACCTTATTATCC
AATATTTTTATTATCAGGTTTTAGG
sequence update 2003. 5. 2
Translated Amino Acid sequence 
NNTNNTNNIEMDDNKSINNNNNNINSINSMYRGTSNLLNPLPFHHTNQQRNGGGSNNHQI
NNHHKDEDEEIDEDEEKFIHKNENFTDFIPRLSKDWFAERSKYIPIRLNIRERNDLRLLE
AALHVSEYTDKIDIIHVGGSKSKRINDQLRGMCAILSGLLVASDFKKGQQLVENKDFFEN
EEFFQNVFEIGRRHKIMNPAKMRTEYGKMIHLLQDAADEDVKKNLSGLNMIKPLKTVFLF
LDERNGLKLLEDPLLELATREVLAENKTRPQIQREIREKEHAIKTLSRRYSSSMLKSEG-
--

---LKEYFSPNQSERGFSLALEHGIGGARLSHNHRKQYNYVYQSLSLWQHILHDMFKLWY
FAEIDLLCGHRYQLSNTGQGLNRIQQCPNVGKIMHSILRQTQKKVGDDWVGSSVIHLGDH
NVPNSLVFIDKYTQISRILSPIVLTIEYIPKITDPNLVAYIKNTFGGPQTLTKIILCDFF
KHAFDGSGADNFFDAGSCIDGRLTSAWQWSAQIAKKPYYPIFLLSGFR
Translated Amino Acid sequence (All Frames) 
Frame A:
**yqqh*qh*ng***ky**qqqqh*qyk*yv*rh**ftqsitfssy*stt*wwgf**ssn
q*sp*r*r*rd**grgeihsqe*kfyrfysktfkglvrrki*iysnsfkys*ek*sssfr
gstsrfriyr*nryhscwwfkikknk*sikryvcnfirfigck*f*krstis*k*rfl*k
*rilskcf*nw*ktqdyesskden*iw*ndsfitrcsr*rckeks*wt*yd*tienrflv
fg*t*rtqivrgsivrigns*gfsre*nqtsnst*ns*kgtcnqniitslliinveirgr
---

---LKEYFSPNQSERGFSLALEHGIGGARLSHNHRKQYNYVYQSLSLWQHILHDMFKLWY
FAEIDLLCGHRYQLSNTGQGLNRIQQCPNVGKIMHSILRQTQKKVGDDWVGSSVIHLGDH
NVPNSLVFIDKYTQISRILSPIVLTIEYIPKITDPNLVAYIKNTFGGPQTLTKIILCDFF
KHAFDGSGADNFFDAGSCIDGRLTSAWQWSAQIAKKPYYPIFLLSGFR

Frame B:
NNTNNTNNIEMDDNKSINNNNNNINSINSMYRGTSNLLNPLPFHHTNQQRNGGGSNNHQI
NNHHKDEDEEIDEDEEKFIHKNENFTDFIPRLSKDWFAERSKYIPIRLNIRERNDLRLLE
AALHVSEYTDKIDIIHVGGSKSKRINDQLRGMCAILSGLLVASDFKKGQQLVENKDFFEN
EEFFQNVFEIGRRHKIMNPAKMRTEYGKMIHLLQDAADEDVKKNLSGLNMIKPLKTVFLF
LDERNGLKLLEDPLLELATREVLAENKTRPQIQREIREKEHAIKTLSRRYSSSMLKSEG-
--

---srntlvqinqrevfhwlwnmalvvhvfltiivnnitmyinhspcgniffmiclnfgt
lqklifyadtdinyqtlvkv*ivfnnvqtlvklcivfyvklkkklvmiglvlplfiwvii
tfqtlwfssintlksles*vqsf*plnifqrspiqi*lltsktplvvhkl*pkssfvisl
ntlsmvvvpitfsmlalvlmvd*lllgngllksqrnliiqyfyyqvl

Frame C:
iipttlttlkwmiikvlitttttltv*ivciealviysihylfiilinnvmvgvliiiks
iitikmkmkrlmrtrrnsftrmkilqilfqdfqrigsqkdlnifqfv*ifvremifvf*r
qhftfqniqiksisfmlvvqnqke*min*evcvqfyqvywlqvilkkvnn*lkikislkm
knsfkmflklvedtrl*iqqr*elnmvk*fiyykmqqmkm*rkilvdli*lnh*kpfscf
wmnvtdsnc*rihc*nwqlvrf*qrikpdlkfnvkfvkrnmqskhyhvvthhqc*nqre-
--

---qgil*sksirerffigfgtwhwwctsfsqss*ti*lcisitllvatyss*yv*tlvl
crn*sfmrtqisiikhwsrsksystmskrw*nya*ystsnskksw**lgwffrysfg*s*
rsklfgfhr*ihsnl*npksnrfnh*iyskdhrskfsclhqkhlwwstnfnqnhpl*fl*
trfrw*wcr*lfrcwlly*w*infclamvcsnrketllsnifiirf*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF455 (AFF455Q) /CSM/AF/AFF4-C/AFF455Q.Seq.d/                       3035   0.0
CHL491 (CHL491Q) /CSM/CH/CHL4-D/CHL491Q.Seq.d/                       1291   0.0
CHL110 (CHL110Q) /CSM/CH/CHL1-A/CHL110Q.Seq.d/                       1291   0.0
CHI139 (CHI139Q) /CSM/CH/CHI1-B/CHI139Q.Seq.d/                       1291   0.0
AHM816 (AHM816Q) /CSM/AH/AHM8-A/AHM816Q.Seq.d/                       1291   0.0
VHI541 (VHI541Q) /CSM/VH/VHI5-B/VHI541Q.Seq.d/                       1289   0.0
VHK328 (VHK328Q) /CSM/VH/VHK3-B/VHK328Q.Seq.d/                       1283   0.0
CHR363 (CHR363Q) /CSM/CH/CHR3-C/CHR363Q.Seq.d/                       1283   0.0
CHD280 (CHD280Q) /CSM/CH/CHD2-D/CHD280Q.Seq.d/                       1283   0.0
AHA156 (AHA156Q) /CSM/AH/AHA1-C/AHA156Q.Seq.d/                       1273   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    46   1e-08  14
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    32   3e-07  19
AL732436|AL732436.6 Zebrafish DNA sequence from clone BUSM1-21A14 in linkage group 9  Contains part of a novel gene and a CpG island.    68   4e-07  1
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            40   5e-07  29
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    36   3e-06  9
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    38   3e-06  15
AC115682|AC115682.2 Dictyostelium discoideum chromosome 2 map 4101105-4158643 strain AX4, complete sequence.    36   5e-06  8
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    36   3e-05  15
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.    38   6e-05  19
BX004834|BX004834.12 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-42E22.    46   8e-05  8
dna update 2004. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR382131_1131(CR382131|pid:none) Yarrowia lipolytica strain CLIB...   388   e-106
CP001142_478(CP001142|pid:none) Phaeodactylum tricornutum CCAP 1...   385   e-105
CR382125_995(CR382125|pid:none) Kluyveromyces lactis strain NRRL...   347   7e-94
AE016819_283(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   346   1e-93
CR380956_239(CR380956|pid:none) Candida glabrata strain CBS138 c...   343   1e-92
CP000081_414(CP000081|pid:none) Leishmania major chromosome 35, ...   286   1e-75
AM502253_428(AM502253|pid:none) Leishmania infantum chromosome 35.    285   4e-75
AM494971_416(AM494971|pid:none) Leishmania braziliensis chromoso...   281   3e-74
CR940353_528(CR940353|pid:none) Theileria annulata strain Ankara...   262   3e-68
AL844507_193(AL844507|pid:none) Plasmodium falciparum 3D7 chromo...   242   3e-62
protein update 2009. 3.28
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFF455 is cyt
5' end seq. ID AFF455F
5' end seq. 
>AFF455F.Seq
TAATAATACCAACAACACTAACAACATTGAAATGGATGATAATAAAAGTATTAATAACAA
CAACAACAACATTAACAGTATAAATAGTATGTATAGAGGCACTAGTAATTTACTCAATCC
ATTACCTTTTCATCATACTAATCAACAACGTAATGGTGGGGGTTCTAATAATCATCAAAT
CAATAATCACCATAAAGATGAAGATGAAGAGATTGATGAGGACGAGGAGAAATTCATTCA
CAAGAATGAAAATTTTACAGATTTTATTCCAAGACTTTCAAAGGATTGGTTCGCAGAAAG
ATCTAAATATATTCCAATTCGTTTAAATATTCGTGAGAGAAATGATCTTCGTCTTTTAGA
GGCAGCACTTCACGTTTCAGAATATACAGATAAAATCGATATCATTCATGTTGGTGGTTC
AAAATCAAAAAGAATAAATGATCAATTAAGAGGTATGTGTGCAATTTTATCAGGTTTATT
GGTTGCAAGTGATTTTAAAAAAGGTCAACAATTAGTTGAAAATAAAGATTTCTTTGAAAA
TGAAGAATTCTTTCAAAATGTTTTTGAAATTGGTAGAAGACACAAGATTATGAATCCAGC
AAAGATGAGAACTGAATATGGTAAAATGATTCATTTATTACAAGATGCAGCAGATGAAGA
TGTAAAGAAAAATCTTAGTGGACTTAATATGATTAAACCATTGAAAACCGTTTTCTTGTT
TTTGGATGAACGTAACGGACTCAAATTGTTAGAGGATCCATTGTTAGAATTGGCAACTCG
TGAGGTTTTAGCAGAGAATAAAACCAGACCTCAAATTCAACGTGAAATTCGTGAAAAGGA
ACATGCAATCAAAACATTATCACGTCGTTACTCATCATCAATGTTGAAATCAGAGGGAGG
NNNNNNNNNN
Length of 5' end seq. 910
3' end seq. ID AFF455Z
3' end seq. 
>AFF455Z.Seq
----------CTCAAGGAATACTTTAGTCCAAATCAATCAGAGAGAGGTTTTTCATTGGC
TTTGGAACATGGCATTGGTGGTGCACGTCTTTCTCACAATCATCGTAAACAATATAACTA
TGTATATCAATCACTCTCCTTGTGGCAACATATTCTTCATGATATGTTTAAACTTTGGTA
CTTTGCAGAAATTGATCTTTTATGCGGACACAGATATCAATTATCAAACACTGGTCAAGG
TCTAAATCGTATTCAACAATGTCCAAACGTTGGTAAAATTATGCATAGTATTCTACGTCA
AACTCAAAAAAAAGTTGGTGATGATTGGGTTGGTTCTTCCGTTATTCATTTGGGTGATCA
TAACGTTCCAAACTCTTTGGTTTTCATCGATAAATACACTCAAATCTCTAGAATCCTAAG
TCCAATCGTTTTAACCATTGAATATATTCCAAAGATCACCGATCCAAATTTAGTTGCTTA
CATCAAAAACACCTTTGGTGGTCCACAAACTTTAACCAAAATCATCCTTTGTGATTTCTT
TAAACACGCTTTCGATGGTAGTGGTGCCGATAACTTTTTCGATGCTGGCTCTTGTATTGA
TGGTAGATTAACTTCTGCTTGGCAATGGTCTGCTCAAATCGCAAAGAAACCTTATTATCC
AATATTTTTATTATCAGGTTTTAGG
Length of 3' end seq. 675
Connected seq. ID AFF455P
Connected seq. 
>AFF455P.Seq
TAATAATACCAACAACACTAACAACATTGAAATGGATGATAATAAAAGTATTAATAACAA
CAACAACAACATTAACAGTATAAATAGTATGTATAGAGGCACTAGTAATTTACTCAATCC
ATTACCTTTTCATCATACTAATCAACAACGTAATGGTGGGGGTTCTAATAATCATCAAAT
CAATAATCACCATAAAGATGAAGATGAAGAGATTGATGAGGACGAGGAGAAATTCATTCA
CAAGAATGAAAATTTTACAGATTTTATTCCAAGACTTTCAAAGGATTGGTTCGCAGAAAG
ATCTAAATATATTCCAATTCGTTTAAATATTCGTGAGAGAAATGATCTTCGTCTTTTAGA
GGCAGCACTTCACGTTTCAGAATATACAGATAAAATCGATATCATTCATGTTGGTGGTTC
AAAATCAAAAAGAATAAATGATCAATTAAGAGGTATGTGTGCAATTTTATCAGGTTTATT
GGTTGCAAGTGATTTTAAAAAAGGTCAACAATTAGTTGAAAATAAAGATTTCTTTGAAAA
TGAAGAATTCTTTCAAAATGTTTTTGAAATTGGTAGAAGACACAAGATTATGAATCCAGC
AAAGATGAGAACTGAATATGGTAAAATGATTCATTTATTACAAGATGCAGCAGATGAAGA
TGTAAAGAAAAATCTTAGTGGACTTAATATGATTAAACCATTGAAAACCGTTTTCTTGTT
TTTGGATGAACGTAACGGACTCAAATTGTTAGAGGATCCATTGTTAGAATTGGCAACTCG
TGAGGTTTTAGCAGAGAATAAAACCAGACCTCAAATTCAACGTGAAATTCGTGAAAAGGA
ACATGCAATCAAAACATTATCACGTCGTTACTCATCATCAATGTTGAAATCAGAGGGAGG
----------CTCAAGGAATACTTTAGTCCAAATCAATCAGAGAGAGGTTTTTCATTGGC
TTTGGAACATGGCATTGGTGGTGCACGTCTTTCTCACAATCATCGTAAACAATATAACTA
TGTATATCAATCACTCTCCTTGTGGCAACATATTCTTCATGATATGTTTAAACTTTGGTA
CTTTGCAGAAATTGATCTTTTATGCGGACACAGATATCAATTATCAAACACTGGTCAAGG
TCTAAATCGTATTCAACAATGTCCAAACGTTGGTAAAATTATGCATAGTATTCTACGTCA
AACTCAAAAAAAAGTTGGTGATGATTGGGTTGGTTCTTCCGTTATTCATTTGGGTGATCA
TAACGTTCCAAACTCTTTGGTTTTCATCGATAAATACACTCAAATCTCTAGAATCCTAAG
TCCAATCGTTTTAACCATTGAATATATTCCAAAGATCACCGATCCAAATTTAGTTGCTTA
CATCAAAAACACCTTTGGTGGTCCACAAACTTTAACCAAAATCATCCTTTGTGATTTCTT
TAAACACGCTTTCGATGGTAGTGGTGCCGATAACTTTTTCGATGCTGGCTCTTGTATTGA
TGGTAGATTAACTTCTGCTTGGCAATGGTCTGCTCAAATCGCAAAGAAACCTTATTATCC
AATATTTTTATTATCAGGTTTTAGG
Length of connected seq. 1575
Full length Seq ID -
Full length Seq. -
Length of full length seq. -