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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF451
Library AF
(Link to library)
Clone ID AFF451
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16173-1
Original site URL
Representative seq. ID AFF451P
(Link to Original site)
Representative DNA sequence 
>AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/
AGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGA
CCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGAT
GGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAA
TTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCA
GCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATT
ATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCA
GTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCA
TCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACT
CGTGTGAAAACCATCGATTACAAAGGTGACGTAAAATATCCAATCAATGCCATCAACATT
TTGAAAGCCCACGGAAAGAGTGCAAAAGAGAGTACCCTCGTXXXXXXXXXXGGTTGTATG
GCCGTTCGTCGTTGTAAAAAAGAAGATCTTAAACGTATCGCCAAATCATGTGGTGGTACA
GTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGT
ATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAAC
TCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAA
ATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACC
ATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCC
GCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTT
GTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTA
AAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGT
CTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCC
ATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATC
GATGATAAAATCACTCTTAATCCAAGAGAACAACAAGGGGNNATCATAAGGTC
sequence update 2001. 6. 2
Translated Amino Acid sequence 
RAQNVLAVTAIANIVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQ
LSELQDQEVGDGTTTVVILAAELLKRANELVARKVHPTVIISGFRLACTEAIKYINETLA
VKVETLPKDFIVNIAKTSMSSKTINDDSDFFSKIVIEAITRVKTIDYKGDVKYPINAINI
LKAHGKSAKESTL---

---GCMAVRRCKKEDLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDE
LIIVKNSNKKSASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSI
YLDNIAATMGSRKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKK
SYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQGX
IIR
Translated Amino Acid sequence (All Frames) 
Frame A:
RAQNVLAVTAIANIVKTSFGPIGLDKMLIDNIGSIVVTNDGATILQKIDIEHPAAKILVQ
LSELQDQEVGDGTTTVVILAAELLKRANELVARKVHPTVIISGFRLACTEAIKYINETLA
VKVETLPKDFIVNIAKTSMSSKTINDDSDFFSKIVIEAITRVKTIDYKGDVKYPINAINI
LKAHGKSAKESTL---

---GCMAVRRCKKEDLKRIAKSCGGTVLITLANLEGEESFDTTALGIADEVVQDRLADDE
LIIVKNSNKKSASIILRGANELMLDEMERSIHDSLCIVKRTLESGTIVPGGGAVESALSI
YLDNIAATMGSRKQLAISEFAESLLVVPKQLAVNAALDASDLVSKLKAYHHAAQTDPSKK
SYAYSGLDLFNNKVRNNLEAGVLEPAIAKIKCIKFATESAITILRIDDKITLNPREQQGX
IIR

Frame B:
ehkmf*qlqqlqi*lkhhldqlv*ikc*liildqlllpmmvqpfykrli*niqqqrf*yn
yqnckikklvmvqlql*f*qqny*nvpmnw*hvkyiqqlllvvld*hvqkqsnistkh*q
lklkpyqrisl*tlprhqchqrpsmtivisfqrlslrqslv*kpsitkvt*niqsmpstf
*kptervqkrvps---

---vvwpfvvvkkkilnvspnhvvvqs*shlqisrvknhlipppwvlpmklfkidwpmmn
*slsktqirnqlqsffvvpmn*cwmkwnvpfmtlsvs*rel*nleplfqvvvqlnlpsls
isiilppqwvvvnn*qslnllnpswlyqnnwl*mlllmpli*fqn*kpiimplklihqrn
lthtlvsiysitkleii*rlvf*nlplpksnvsnlqlkalspsfvsmikslliqennkgx
s*g

Frame C:
stkcfssysnckys*niiwtnwfr*nvn**ywinccyq*wcnhftkd*yrtssskdfsti
iriarsrsw*wynyscnfssriietcq*igst*stsnsyy*wf*ismyrsnqiyqrniss
*s*nltkgfhckhcqdinvikdhq*r**flfkdch*gnhscenhrlqr*rkisnqchqhf
esprkeckreypr---

---lygrssl*krrs*tyrqimwwyslnhtcksrg*rii*yhrlgycr*scsr*igr**i
nhcqklk*eisfnhsswcq*invg*ngtfhs*lslyrkenfriwnhcsrwwcs*icplyl
sr*ycrhng*s*tisnl*ic*iplgctktigckccs*cl*fsfkikslsscrsn*sikei
lrilwsrfiq*qs*k*frgwcfrtchcqnqmyqicn*kryhhpsyr**nhs*skrttrgx
hkv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF451 (AFF451Q) /CSM/AF/AFF4-C/AFF451Q.Seq.d/                       2531   0.0
CFH434 (CFH434Q) /CSM/CF/CFH4-B/CFH434Q.Seq.d/                       1402   0.0
AFD439 (AFD439Q) /CSM/AF/AFD4-B/AFD439Q.Seq.d/                       1402   0.0
AFG366 (AFG366Q) /CSM/AF/AFG3-C/AFG366Q.Seq.d/                       1326   0.0
VFD589 (VFD589Q) /CSM/VF/VFD5-D/VFD589Q.Seq.d/                       1318   0.0
VFF578 (VFF578Q) /CSM/VF/VFF5-D/VFF578Q.Seq.d/                       1263   0.0
VFA730 (VFA730Q) /CSM/VF/VFA7-B/VFA730Q.Seq.d/                       1225   0.0
VFL307 (VFL307Q) /CSM/VF/VFL3-A/VFL307Q.Seq.d/                       1221   0.0
AHI886 (AHI886Q) /CSM/AH/AHI8-D/AHI886Q.Seq.d/                       1187   0.0
FC-AG03 (FC-AG03Q) /CSM/FC/FC-AG/FC-AG03Q.Seq.d/                     1183   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB008158|AB008158.1 Dictyostelium discoideum DdTcp-1 mRNA for t-complex polypeptide 1 homologue, complete cds.  1402   0.0    1
AZ530054|AZ530054.1 ENTDW96TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    66   2e-14  2
AZ526360|AZ526360.1 254PbF04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    70   6e-14  3
AZ522254|AZ522254.1 204PbE04 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    68   3e-12  2
BF298951|BF298951.1 068PbG10 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   3e-12  2
BF299139|BF299139.1 069PbF12 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    68   3e-12  2
AX488977|AX488977.1 Sequence 6277 from Patent WO02053728.          42   1e-10  5
AL437502|AL437502.1 T3 end of clone BC0AA009G02 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    48   8e-10  4
U43123|U43123.1 Naegleria fowleri T-complex polypeptide homolog mRNA, partial cds.    38   6e-09  5
AF143494|AF143494.1 Xenopus laevis t-complex polypeptide 1 (TCP1) mRNA, complete cds.    48   3e-08  5
dna update 2004. 4.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB008158_1(AB008158|pid:none) Dictyostelium discoideum DdTcp-1 m...   454   e-126
EU965648_1(EU965648|pid:none) Zea mays clone 287725 T-complex pr...   357   4e-97
AF442545_1(AF442545|pid:none) Physarum polycephalum CCT chaperon...   349   1e-94
BT002052_1(BT002052|pid:none) Arabidopsis thaliana t-complex pol...   324   4e-87
AM502250_384(AM502250|pid:none) Leishmania infantum chromosome 32.    324   5e-87
AC152752_1(AC152752|pid:none) Medicago truncatula clone mth2-89c...   323   6e-87
AP000383_4(AP000383|pid:none) Arabidopsis thaliana genomic DNA, ...   318   2e-85
(P18279) RecName: Full=T-complex protein 1 subunit alpha;       ...   301   4e-80
AY891878_1(AY891878|pid:none) Synthetic construct Homo sapiens c...   300   7e-80
(Q4R5G2) RecName: Full=T-complex protein 1 subunit alpha;       ...   300   7e-80
protein update 2009. 4.20
PSORT 

 psg: 0.93  gvh: 0.40  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  8.0 %: mitochondrial

>> prediction for AFF451 is nuc
5' end seq. ID AFF451F
5' end seq. 
>AFF451F.Seq
AGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGA
CCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGAT
GGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAA
TTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCA
GCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATT
ATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCA
GTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCA
TCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACT
CGTGTGAAAACCATCGATTACAAAGGTGACGTAAAATATCCAATCAATGCCATCAACATT
TTGAAAGCCCACGGAAAGAGTGCAAAAGAGAGTACCCTCGT----------
Length of 5' end seq. 581
3' end seq. ID AFF451Z
3' end seq. 
>AFF451Z.Seq
----------GGTTGTATGGCCGTTCGTCGTTGTAAAAAAGAAGATCTTAAACGTATCGC
CAAATCATGTGGTGGTACAGTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATCATT
TGATACCACCGCCTTGGGTATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGATGA
ATTAATCATTGTCAAAAACTCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAA
TGAATTAATGTTGGATGAAATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAAGAG
AACTTTAGAATCTGGAACCATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTAT
CTATCTCGATAATATTGCCGCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGAATT
TGCTGAATCCCTCTTGGTTGTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGCCTC
TGATTTAGTTTCAAAATTAAAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAAGAA
ATCTTACGCATACTCTGGTCTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGAGGC
TGGTGTTTTAGAACCTGCCATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAGCGC
TATCACCATCCTTCGTATCGATGATAAAATCACTCTTAATCCAAGAGAACAACAAGGGGN
NATCATAAGGTC
Length of 3' end seq. 722
Connected seq. ID AFF451P
Connected seq. 
>AFF451P.Seq
AGAGCACAAAATGTTTTAGCAGTTACAGCAATTGCAAATATAGTTAAAACATCATTTGGA
CCAATTGGTTTAGATAAAATGTTAATTGATAATATTGGATCAATTGTTGTTACCAATGAT
GGTGCAACCATTTTACAAAAGATTGATATAGAACATCCAGCAGCAAAGATTTTAGTACAA
TTATCAGAATTGCAAGATCAAGAAGTTGGTGATGGTACAACTACAGTTGTAATTTTAGCA
GCAGAATTATTGAAACGTGCCAATGAATTGGTAGCACGTAAAGTACATCCAACAGTTATT
ATTAGTGGTTTTAGATTAGCATGTACAGAAGCAATCAAATATATCAACGAAACATTAGCA
GTTAAAGTTGAAACCTTACCAAAGGATTTCATTGTAAACATTGCCAAGACATCAATGTCA
TCAAAGACCATCAATGACGATAGTGATTTCTTTTCAAAGATTGTCATTGAGGCAATCACT
CGTGTGAAAACCATCGATTACAAAGGTGACGTAAAATATCCAATCAATGCCATCAACATT
TTGAAAGCCCACGGAAAGAGTGCAAAAGAGAGTACCCTCGT----------GGTTGTATG
GCCGTTCGTCGTTGTAAAAAAGAAGATCTTAAACGTATCGCCAAATCATGTGGTGGTACA
GTCTTAATCACACTTGCAAATCTCGAGGGTGAAGAATCATTTGATACCACCGCCTTGGGT
ATTGCCGATGAAGTTGTTCAAGATAGATTGGCCGATGATGAATTAATCATTGTCAAAAAC
TCAAATAAGAAATCAGCTTCAATCATTCTTCGTGGTGCCAATGAATTAATGTTGGATGAA
ATGGAACGTTCCATTCATGACTCTCTCTGTATCGTAAAGAGAACTTTAGAATCTGGAACC
ATTGTTCCAGGTGGTGGTGCAGTTGAATCTGCCCTCTCTATCTATCTCGATAATATTGCC
GCCACAATGGGTAGTCGTAAACAATTAGCAATCTCTGAATTTGCTGAATCCCTCTTGGTT
GTACCAAAACAATTGGCTGTAAATGCTGCTCTTGATGCCTCTGATTTAGTTTCAAAATTA
AAAGCCTATCATCATGCCGCTCAAACTGATCCATCAAAGAAATCTTACGCATACTCTGGT
CTCGATTTATTCAATAACAAAGTTAGAAATAATTTAGAGGCTGGTGTTTTAGAACCTGCC
ATTGCCAAAATCAAATGTATCAAATTTGCAACTGAAAGCGCTATCACCATCCTTCGTATC
GATGATAAAATCACTCTTAATCCAAGAGAACAACAAGGGGNNATCATAAGGTC
Length of connected seq. 1303
Full length Seq ID -
Full length Seq. -
Length of full length seq. -