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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF439
Library AF
(Link to library)
Clone ID AFF439
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15531-1
Original site URL
Representative seq. ID AFF439P
(Link to Original site)
Representative DNA sequence 
>AFF439 (AFF439Q) /CSM/AF/AFF4-B/AFF439Q.Seq.d/
GTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGC
TAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAG
AAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGT
TGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCA
TAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGA
AAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGT
TTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTAC
TATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTAT
TGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGC
CAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTACGTTGTAGC
AATCTCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGTXXXXXXXXXXCTTTCAN
CAATATTCAATCATACATTGAACACGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGG
TGGTAAATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGA
TCCACATTACAAGTCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTA
TGAAGATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAAACTTCACCATATGCTTT
AACTGGTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACCGCTCACAAATACTTGAA
GAATGCTGCTGGTAACTTTTATATCAATGATAAATGCACTGGTGCCGTCGTTGGTCAACA
ACCATTTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTCATCATTAAATCT
TTTAAGATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTCCCACTCACTAGTTTCAC
TTATCCATACAGATGATCCAGAAAACTAAAAATTTNAACTAAATAAATACAAAANA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
fhfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCV
VGGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAV
FLKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPA
NSAGCWNILTYQPLEGYVVAISPFNFTAIGLNLS---

---FXNIQSYIEHAKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVL
TIYVYEDSKFEETLKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGA
VVGQQPFGGSRASGTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYR*srklkixtk*
iqx
Translated Amino Acid sequence (All Frames) 
Frame A:
vslqtlkkhiksthvslcks*hpntg*ptnvkl*swfnt*kiikrsmc*i*khnnrysmc
cww*rnsyw*cskainll*s**sighlpssqcritqisr*kfhgi*spmgiiti*skkcs
fp*scrfvkyqvpl*clsfyyawsr*nclasrn*cssrry*fppfqc*irsrnlptttts
qfcrllehfnlstirglrcsnlti*fhsnwfefk---

---lsxifnhtlntlklhqmlkslsvvnviallvgllnqlsslpkihitsqwkkkfsvqs
*pfmfmkilnlkkh*ksvmklhhml*lvqsfqlvdmllkpltnt*rmllvtfisminalv
pslvnnhlvvlvlqvqtiklvhh*if*dgsapeqskktlshslvslihtddpen*kfxln
kykx

Frame B:
fhfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIPCV
VGGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARSAV
FLKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQPPA
NSAGCWNILTYQPLEGYVVAISPFNFTAIGLNLS---

---fxqysiih*tr*sftrc*nhcrw*m**lcwlvc*tnyhpcqrstlqvngrrnfrssl
nhlcl*rf*i*rniknl**nfticfnwfnlfnl*icy*nrsqileeccw*llyq**mhwc
rrwsttiwwfscfrykr*swfiiksfkmdqrqnnqrklcpth*fhlsiqmiqktknxn*i
ntk

Frame C:
ftsnikkthqeyscltlqeltsqhrltnqc*imklvqhieny*kkhvlnletqqsifhvl
lvvkkfvlvmfksn*faliiikywppsikpmpnysn*plkipwnlkpngnhyhlkqevqf
slklqic*ipstvmms*lllclvkvklfgkqklmqqqkvlissvsmlntlkkftnnnhqp
ilqavgtf*pinh*rvtl*qshhlisqqlv*i*---

---FXNIQSYIEHAKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVL
TIYVYEDSKFEETLKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGA
VVGQQPFGGSRASGTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYR*srklkixtk*
iqx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF439 (AFF439Q) /CSM/AF/AFF4-B/AFF439Q.Seq.d/                       2254   0.0
VFN208 (VFN208Q) /CSM/VF/VFN2-A/VFN208Q.Seq.d/                       1225   0.0
AFD340 (AFD340Q) /CSM/AF/AFD3-B/AFD340Q.Seq.d/                       1225   0.0
AFJ501 (AFJ501Q) /CSM/AF/AFJ5-A/AFJ501Q.Seq.d/                       1219   0.0
SLH659 (SLH659Q) /CSM/SL/SLH6-C/SLH659Q.Seq.d/                       1207   0.0
VFE114 (VFE114Q) /CSM/VF/VFE1-A/VFE114Q.Seq.d/                       1205   0.0
AFN524 (AFN524Q) /CSM/AF/AFN5-A/AFN524Q.Seq.d/                       1189   0.0
CHA741 (CHA741Q) /CSM/CH/CHA7-B/CHA741Q.Seq.d/                       1116   0.0
AFO165 (AFO165Q) /CSM/AF/AFO1-C/AFO165Q.Seq.d/                       1114   0.0
AFI131 (AFI131Q) /CSM/AF/AFI1-B/AFI131Q.Seq.d/                       1112   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL031786|AL031786.1 S.pombe chromosome II cosmid c24C6.            88   1e-17  2
D89230|D89230.1 Schizosaccharomyces pombe mRNA, partial cds, clone: SY 1373.    80   9e-11  1
CF192429|CF192429.1 l4g02j2.f1 Cryptococcus neoformans strain B3501 Cryptococcus neoformans var. neoformans cDNA clone l4g02j2 3', mRNA sequence.    70   8e-08  1
AL416406|AL416406.1 T3 end of clone AX0AA014C08 of library AX0AA from strain CBS 7064 of Pichia farinosa.    56   2e-05  2
AC090078|AC090078.2 Trypanosoma cruzi clone GAMFS45 strain CL Brener, *** SEQUENCING IN PROGRESS ***, 12 unordered pieces.    60   8e-05  1
AL393468|AL393468.1 T3 end of clone AR0AA011B08 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    60   8e-05  1
AL416312|AL416312.1 T7 end of clone AX0AA013D07 of library AX0AA from strain CBS 7064 of Pichia farinosa.    48   1e-04  2
AZ932439|AZ932439.1 474.dhz99a06.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz99a06.s1, DNA sequence.    58   3e-04  1
BH391341|BH391341.1 AG-ND-107E4.TF ND-TAM Anopheles gambiae genomic clone AG-ND-107E4, DNA sequence.    56   0.001  1
CC296277|CC296277.1 CH261-34M22_RM1.2 CH261 Gallus gallus genomic clone CH261-34M22, DNA sequence.    36   0.015  2
dna update 2004. 4.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RA2) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   418   e-115
CP000140_2188(CP000140|pid:none) Parabacteroides distasonis ATCC...   233   7e-60
CP001087_1672(CP001087|pid:none) Desulfobacterium autotrophicum ...   230   8e-59
EF157670_5(EF157670|pid:none) Uncultured bacterium CBNPD1 BAC cl...   229   2e-58
CU466930_1098(CU466930|pid:none) Candidatus Cloacamonas acidamin...   225   2e-57
AP009380_1401(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...   218   2e-55
(P78568) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   216   1e-54
CU207366_389(CU207366|pid:none) Gramella forsetii KT0803 complet...   215   2e-54
CR522870_2491(CR522870|pid:none) Desulfotalea psychrophila LSv54...   214   4e-54
CP001034_1725(CP001034|pid:none) Natranaerobius thermophilus JW/...   209   2e-52
protein update 2009. 4.20
PSORT 

 psg: 0.75  gvh: 0.46  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: nuclear

>> prediction for AFF439 is mit
5' end seq. ID AFF439F
5' end seq. 
>AFF439F.Seq
GTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGC
TAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAG
AAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGT
TGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCA
TAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGA
AAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGT
TTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTAC
TATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTAT
TGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGC
CAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTACGTTGTAGC
AATCTCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGT----------
Length of 5' end seq. 643
3' end seq. ID AFF439Z
3' end seq. 
>AFF439Z.Seq
----------CTTTCANCAATATTCAATCATACATTGAACACGCTAAAGCTTCACCAGAT
GCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACT
ATCATCCTTGCCAAAGATCCACATTACAAGTCAATGGAAGAAGAAATTTTCGGTCCAGTC
TTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAA
ACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACC
GCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTATATCAATGATAAATGCACTGGT
GCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCT
GGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTC
CCACTCACTAGTTTCACTTATCCATACAGATGATCCAGAAAACTAAAAATTTNAACTAAA
TAAATACAAAANA
Length of 3' end seq. 543
Connected seq. ID AFF439P
Connected seq. 
>AFF439P.Seq
GTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCAAGAGC
TAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACACATAG
AAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCATGTGT
TGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCTGATCA
TAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCCGTTGA
AAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGTGCAGT
TTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCTTCTAC
TATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAAGGTAT
TGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCACCAGC
CAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAGAGGGTTACGTTGTAGC
AATCTCACCATTTAATTTCACAGCAATTGGTTTGAATTTAAGT----------CTTTCAN
CAATATTCAATCATACATTGAACACGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGG
TGGTAAATGTGATAGCTCTGTTGGTTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGA
TCCACATTACAAGTCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTA
TGAAGATTCTAAATTTGAAGAAACATTAAAAATCTGTGATGAAACTTCACCATATGCTTT
AACTGGTTCAATCTTTTCAACTTGTAGATATGCTATTGAAACCGCTCACAAATACTTGAA
GAATGCTGCTGGTAACTTTTATATCAATGATAAATGCACTGGTGCCGTCGTTGGTCAACA
ACCATTTGGTGGTTCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTCATCATTAAATCT
TTTAAGATGGATCAGCGCCAGAACAATCAAAGAAAACTTTGTCCCACTCACTAGTTTCAC
TTATCCATACAGATGATCCAGAAAACTAAAAATTTNAACTAAATAAATACAAAANA
Length of connected seq. 1186
Full length Seq ID -
Full length Seq. -
Length of full length seq. -