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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHC469
Library CH
(Link to library)
Clone ID CHC469
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16513-1
Original site URL
Representative seq. ID CHC469E
(Link to Original site)
Representative DNA sequence 
>CHC469 (CHC469Q) /CSM/CH/CHC4-C/CHC469Q.Seq.d/
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATGCCACAAAGAATGAAAATATTA
TTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTT
GTTGTTTTATCACCAGACAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTA
AAATTCTATGCACCATGGTGTGGCCATTGTAAAAAACTTGCACCACATTTTGAAATCCTT
GCTGACACATTCGCACCAGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAA
GCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATC
TTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTA
ACTTATATCAATAATCACGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTT
GTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTA
CTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATC
CTTGGTAACACNTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCT
GCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGG
TTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTC
ATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTT
GGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCT
GAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAA
TTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATC
GATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTNCGGA
AAGAAAGCTGTATGAATTTGCCAAG
sequence update 2002.10.27
Translated Amino Acid sequence 
tvglldfiy**YMPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFV
KFYAPWCGHCKKLAPHFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKI
FDKSTTAKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVL
LEFYAPWCGHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKW
FGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAA
EVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMXG
KKAV*icq
Translated Amino Acid sequence (All Frames) 
Frame A:
tvglldfiy**YMPQRMKILLFVTLIALAFVALCSAEGNVVVLSPDNFDTVVDGSKTVFV
KFYAPWCGHCKKLAPHFEILADTFAPVSNKVVIAKVDCDQADNKALCSKYDVSGYPTLKI
FDKSTTAKDYNGARSVDELLTYINNHAKTNVKVKKAPSNVVDLSPSNFDSVVLDKSKNVL
LEFYAPWCGHCKKLMPDYEILGNTYANEKDVVIAKIDCDAADNKAICSKYGVTGFPTLKW
FGKQSKDGEKYEQGRDLDTFINYINKQAGVNRVKGGKLAVGAGRVEQLDTIATEFIAAAA
EVRKELVKKAQTVVDSLPEELRTEGSYYVKVMKTIAEKSIDFVTTEIARITKLVSGSMXG
KKAV*icq


Frame B:
llaywisfinntchke*kyyyllh*lhwhllpyaqqkemllfyhqttliqllmvqklyl*
nsmhhgvaivknlhhilksllthshqfptkl*lpklivtklttklsvvnmmflviqpsks
ltnqplpkittvldqlmny*lisiitpkptlk*rkhhqmlliyhhqiliqlf*inqrmfy
lnsmhhgvviaks*cqimkslvtxtpmkkmlsspkliamllitkpsapnmvslvsqhsng
svnkaktvknmnkvvililssttstnklvltvskvvnsllvlvvlnnsillplnsslpll
ksvrnssrklkpslilsqkn*elkvhttsks*kpllknqsil*plkslvspn*slvqcxe
rklyefak


Frame C:
cwptgfhlliihatkneniiicyincigicclmlsrrkcccfitrql*ysc*wfkncick
ilctmvwpl*ktcttf*npc*hirtsfqqscncqs*l*ps*qqssl**i*cfwlsnpqnl
*qinhcqrlqrc*is**iinlyq*srqnqr*skestikcc*fitikf*fscfr*ikecft
*ilctmvwslqkvnarl*npw*hxrq*krcchrqn*lrcc**qshllqiwchwfpntqmv
r*tkqrr*ki*trs*s*yfhqlhqqtswc*pcqrw*trcwcwsc*ttryych*ihrcrc*
sp*gtrqessnrr*fsprrikn*rfilrqshenhc*kinrfcnh*nrsyhqislwfnvrk
escmnlp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHC469 (CHC469Q) /CSM/CH/CHC4-C/CHC469Q.Seq.d/                       2179   0.0
VFF762 (VFF762Q) /CSM/VF/VFF7-C/VFF762Q.Seq.d/                       2091   0.0
VFF615 (VFF615Q) /CSM/VF/VFF6-A/VFF615Q.Seq.d/                       2091   0.0
SFI534 (SFI534Q) /CSM/SF/SFI5-B/SFI534Q.Seq.d/                       2091   0.0
AHB158 (AHB158Q) /CSM/AH/AHB1-C/AHB158Q.Seq.d/                       2091   0.0
AFO779 (AFO779Q) /CSM/AF/AFO7-D/AFO779Q.Seq.d/                       2091   0.0
VFI769 (VFI769Q) /CSM/VF/VFI7-C/VFI769Q.Seq.d/                       2089   0.0
AFI744 (AFI744Q) /CSM/AF/AFI7-B/AFI744Q.Seq.d/                       2089   0.0
CFF440 (CFF440Q) /CSM/CF/CFF4-B/CFF440Q.Seq.d/                       2085   0.0
AFC888 (AFC888Q) /CSM/AF/AFC8-D/AFC888Q.Seq.d/                       2085   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF019112|AF019112.1 Dictyostelium discoideum protein disulfide isomerase (dis) mRNA, complete cds.  2040   0.0    3
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.  1368   0.0    13
CN640516|CN640516.1 271E09_555565 Douglas-fir cDNA library PmIFG_73-6 Pseudotsuga menziesii var. menziesii cDNA clone Df271-E09 5, mRNA sequence.    66   1e-17  2
BX252003|BX252003.1 Pinus pinaster xylem EST, clone PP058D07.      54   9e-17  4
BQ695627|BQ695627.1 NXPV_030_G06_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_030_G06 5', mRNA sequence.    54   1e-16  3
CV033767|CV033767.1 RTNACL1_36_B08.g1_A029 Roots plus added NaCl Pinus taeda cDNA clone RTNACL1_36_B08_A029 5', mRNA sequence.    54   2e-16  3
CO366743|CO366743.1 RTK1_29_F12.g1_A029 Roots minus potassium Pinus taeda cDNA clone RTK1_29_F12_A029 5', mRNA sequence.    54   3e-16  3
AL663807|AL663807.1 Ciona intestinalis; larval mRNA; EST 3PRIM end of clone 020ZG06 of  library directional larval cDNA library from Ciona intestinalis  (Ciona).    80   1e-14  2
BW082456|BW082456.1 Ciona intestinalis cDNA, clone:rcieg088k11, 3' end, single read.    80   1e-14  2
BW460427|BW460427.1 Ciona intestinalis cDNA, clone:cijv034g19, 3'end, single read.    80   1e-14  2
dna update 2004. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86IA3) RecName: Full=Protein disulfide-isomerase 1;          S...   662   0.0
AF019112_1(AF019112|pid:none) Dictyostelium discoideum protein d...   660   0.0
EF086125_1(EF086125|pid:none) Picea sitchensis clone WS0285_I21 ...   296   8e-79
DQ222488_1(DQ222488|pid:none) Solanum tuberosum clone 098B03 dis...   285   2e-75
AY730725_1(AY730725|pid:none) Entamoeba histolytica protein disu...   283   6e-75
AB182629_1(AB182629|pid:none) Glycine max Gm pdis-1a mRNA for pr...   283   7e-75
(O22263) RecName: Full=Probable protein disulfide-isomerase A6; ...   282   1e-74
AC087425_4(AC087425|pid:none) Oryza sativa (japonica cultivar-gr...   278   2e-73
AM485099_1(AM485099|pid:none) Vitis vinifera contig VV78X160692....   277   4e-73
EU284948_1(EU284948|pid:none) TSA: Elaeis guineensis EgEfMPOB001...   277   5e-73
protein update 2009. 4.12
PSORT 

 psg: 0.98  gvh: 0.77  alm: 0.45  top: 0.13  tms: 0.00  mit: 0.33  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 12.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: vacuolar
	  8.0 %: cytoplasmic
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for CHC469 is exc
5' end seq. ID CHC469F
5' end seq. 
>CHC469F.Seq
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATGCCACAAAGAATGAAAATATTA
TTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTT
GTTGTTTTATCACCAGACAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTA
AAATTCTATGCACCATGGTGTGGCCATTGTAAAAAACTTGCACCACATTTTGAAATCCTT
GCTGACACATTCGCACCAGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAA
GCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATC
TTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTA
ACTTATATCAATAATCACGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTT
GTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTA
CTTGAATTCTATGCACCATGGNGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATC
CTTNNNNNNNNNN
Length of 5' end seq. 613
3' end seq. ID CHC469Z
3' end seq. 
>CHC469Z.Seq
NNNNNNNNNNTNTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAA
ATCNTTGGTAACACNTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGAT
GCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAA
TGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACT
TTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCT
GTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCT
GCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAA
GAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCA
ATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTNC
GGAAAGAAAGCTGTATGAATTTGCCAAG
Length of 3' end seq. 568
Connected seq. ID CHC469P
Connected seq. 
>CHC469P.Seq
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATGCCACAAAGAATGAAAATATTA
TTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTT
GTTGTTTTATCACCAGACAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTA
AAATTCTATGCACCATGGTGTGGCCATTGTAAAAAACTTGCACCACATTTTGAAATCCTT
GCTGACACATTCGCACCAGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAA
GCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATC
TTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTA
ACTTATATCAATAATCACGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTT
GTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTA
CTTGAATTCTATGCACCATGGNGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATC
CTT----------TNTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTAT
GAAATCNTTGGTAACACNTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGC
GATGCTGCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTC
AAATGGTTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGAT
ACTTTCATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTC
GCTGTTGGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCC
GCTGCTGAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCA
GAAGAATTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAA
TCAATCGATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATG
TNCGGAAAGAAAGCTGTATGAATTTGCCAAG
Length of connected seq. 1161
Full length Seq ID CHC469E
Full length Seq. 
>CHC469E.Seq
ACTGTTGGCCTACTGGATTTCATTTATTAATAATACATGCCACAAAGAATGAAAATATTA
TTATTTGTTACATTAATTGCATTGGCATTTGTTGCCTTATGCTCAGCAGAAGGAAATGTT
GTTGTTTTATCACCAGACAACTTTGATACAGTTGTTGATGGTTCAAAAACTGTATTTGTA
AAATTCTATGCACCATGGTGTGGCCATTGTAAAAAACTTGCACCACATTTTGAAATCCTT
GCTGACACATTCGCACCAGTTTCCAACAAAGTTGTAATTGCCAAAGTTGATTGTGACCAA
GCTGACAACAAAGCTCTCTGTAGTAAATATGATGTTTCTGGTTATCCAACCCTCAAAATC
TTTGACAAATCAACCACTGCCAAAGATTACAACGGTGCTAGATCAGTTGATGAATTATTA
ACTTATATCAATAATCACGCCAAAACCAACGTTAAAGTAAAGAAAGCACCATCAAATGTT
GTTGATTTATCACCATCAAATTTTGATTCAGTTGTTTTAGATAAATCAAAGAATGTTTTA
CTTGAATTCTATGCACCATGGTGTGGTCATTGCAAAAAGTTAATGCCAGATTATGAAATC
CTTGGTAACACNTACGCCAATGAAAAAGATGTTGTCATCGCCAAAATTGATTGCGATGCT
GCTGATAACAAAGCCATCTGCTCCAAATATGGTGTCACTGGTTTCCCAACACTCAAATGG
TTCGGTAAACAAAGCAAAGACGGTGAAAAATATGAACAAGGTCGTGATCTTGATACTTTC
ATCAACTACATCAACAAACAAGCTGGTGTTAACCGTGTCAAAGGTGGTAAACTCGCTGTT
GGTGCTGGTCGTGTTGAACAACTCGATACTATTGCCACTGAATTCATCGCTGCCGCTGCT
GAAGTCCGTAAGGAACTCGTCAAGAAAGCTCAAACCGTCGTTGATTCTCTCCCAGAAGAA
TTAAGAACTGAAGGTTCATACTACGTCAAAGTCATGAAAACCATTGCTGAAAAATCAATC
GATTTTGTAACCACTGAAATCGCTCGTATCACCAAATTAGTCTCTGGTTCAATGTNCGGA
AAGAAAGCTGTATGAATTTGCCAAG
Length of full length seq. 1105