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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHC316
Library CH
(Link to library)
Clone ID CHC316
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13873-1
Original site URL
Representative seq. ID CHC316P
(Link to Original site)
Representative DNA sequence 
>CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/
ATTATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGC
TAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGA
AGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGA
ATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAA
TGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAAT
AGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGC
AATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTT
GTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTT
TAATGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCT
AGTACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTXXXXXXXXXXAGA
AGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAAT
TGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGA
TTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAAT
GTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGG
TGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAAT
CATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAA
AACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGA
AGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGA
AACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATC
TGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGA
AGAACTTGAAAAACTTCATAGATCAAATGGTAATTCGTTTGAGTATGCTGGTGAAGAGTT
TTATCATTTTTAATAAAAATTAAAAAAAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
lflif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFE
LRDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNA
IVGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFML
VPIGAPKFKEALRYG---

---EALTLLVDAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSG
EQLMSRYLEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKA
ISEKTVNSLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTG
EIKSGAPCRFERLSKYNRLMKIEEELEKLHRSNGNSFEYAGEEFYHF**klkkn
Translated Amino Acid sequence (All Frames) 
Frame A:
iifniliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*
ikrw**kslfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqc
nrwcingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvya
sthwctki*rsialw---

---rsinsvsgcyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyriltfev*fk*w
*ainv*ifrdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ks
nf*enckfiiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw
*nkiwctl*i*kiiki**inen*rrt*kts*ikw*fv*vcw*rvlsflikikkk

Frame B:
lflif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFE
LRDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNA
IVGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFML
VPIGAPKFKEALRYG---

---EALTLLVDAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSG
EQLMSRYLEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKA
ISEKTVNSLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTG
EIKSGAPCRFERLSKYNRLMKIEEELEKLHRSNGNSFEYAGEEFYHF**klkkn

Frame C:
yf*yfnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln
*emvikvvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmq
slvyqwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*
yplvhqnlkkhcvmv---

---kh*lc*wmlsiiraikmllklvlilllqnfgiqrlqnmiwisri*dphi*slv*ivv
sn*cldi*rf*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkq
flrkl*ihyy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlv
k*nlvhlvdlkdyqniid**klkknlknfidqmvirlsmlvksfiifnkn*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       2480   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1346   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1285   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1283   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1281   0.0
CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       1279   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1279   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1259   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1253   0.0
CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       1243   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   1e-26  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   9e-19  6
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   9e-19  6
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   9e-19  6
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   9e-19  6
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   6e-18  3
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   6e-18  3
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    70   8e-16  2
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   1e-15  2
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   5e-15  4
dna update 2004. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   436   e-121
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   434   e-120
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   432   e-120
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   429   e-119
AY398342_1(AY398342|pid:none) Danio rerio clone RK122A2D02 enola...   428   e-118
BC092869_1(BC092869|pid:none) Danio rerio enolase 3, (beta, musc...   428   e-118
AK290525_1(AK290525|pid:none) Homo sapiens cDNA FLJ77877 complet...   427   e-118
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   427   e-118
(P09104) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   427   e-118
(P17183) RecName: Full=Gamma-enolase;          EC=4.2.1.11; AltN...   427   e-118
protein update 2009. 3.29
PSORT 

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHC316 is nuc
5' end seq. ID CHC316F
5' end seq. 
>CHC316F.Seq
ATTATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGC
TAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGA
AGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGA
ATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAA
TGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAAT
AGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGC
AATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTT
GTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTT
TAATGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCT
AGTACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTTNNNNNNNNNN
Length of 5' end seq. 597
3' end seq. ID CHC316Z
3' end seq. 
>CHC316Z.Seq
NNNNNNNNNNAGAAGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAG
ATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAA
AATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTG
GTGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTG
AAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTA
ATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAG
CAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAA
CTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTC
ATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTG
GTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAA
TGAAAATTGAAGAAGAACTTGAAAAACTTCATAGATCAAATGGTAATTCGTTTGAGTATG
CTGGTGAAGAGTTTTATCATTTTTAATAAAAATTAAAAAAAAAT
Length of 3' end seq. 704
Connected seq. ID CHC316P
Connected seq. 
>CHC316P.Seq
ATTATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGC
TAGAGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGA
AGGAATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGA
ATTAAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAA
TGTAAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAAT
AGATGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGC
AATCGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTT
GTACCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTT
TAATGTTATTAATGGTGGAAAACATGCAGGTAATAGACTACCATTTCAAGAGTTTATGCT
AGTACCCATTGGTGCACCAAAATTTAAAGAAGCATTGCGTTATGGTT----------AGA
AGCATTAACTCTGTTAGTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAAT
TGGTATTGATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGA
TTTCAAGAATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAAT
GTCTAGATATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGG
TGAAGATGATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAAT
CATTGGTGATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAA
AACTGTAAATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGA
AGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGA
AACTGATGATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATC
TGGTGCACCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGA
AGAACTTGAAAAACTTCATAGATCAAATGGTAATTCGTTTGAGTATGCTGGTGAAGAGTT
TTATCATTTTTAATAAAAATTAAAAAAAAAT
Length of connected seq. 1281
Full length Seq ID -
Full length Seq. -
Length of full length seq. -