NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFA144
Library AF
(Link to library)
Clone ID AFA144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16281-1
Original site URL
Representative seq. ID AFA144P
(Link to Original site)
Representative DNA sequence 
>AFA144 (AFA144Q) /CSM/AF/AFA1-B/AFA144Q.Seq.d/
CTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGCCAACCTTAAAAGAAAGAT
TAGTTCAAATCATCCCAGGTAAAATTGAACAAGTTAAACAATTAAAAACTGAACATGGTG
ACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTGGTATGAGATCAGTTAAAT
CATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCATTAGATTCCGTGGTTTAA
CAATCCCAGAATGTCAAGAGAAATTACCAAAAGCTCCAGGTGGTGCTGAACCATTACCAG
AAGGTATTTTATGGTTACTTTTAACTGGTGAAGTTCCAACTGAATCACAAGTTAAAACCT
TATCAAXXXXXXXXXXTATGGCTTAAAANAACANAACTTNTACCCTGTTTTATTCGGNGN
TTCAANAGCCCTTGGNGTTTTATCNCCCTTAGTTTGGGNTCGGATCNTAGGNCCCCCNAT
CGAAAGNCCNAAANCNGNCCCCCCTGAATGGATTTCNTCCTACGNAAACCCNGGCCNATC
AAAAAAATAAATTTAAGNAATAAAATCCAAAAAAAAAAAAATAGATATCGACAAGTAAAA
AATNTTTTTGGTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
NYLKNSGRYFSTEPTLKERLVQIIPGKIEQVKQLKTEHGDKIIGTCTVAQAYGGMRSVKS
LVTETSSLDPEEGIRFRGLTIPECQEKLPKAPGGAEPLPEGILWLLLTGEVPTESQVKTL
S---

---YGLKXXNXYPVLFGXSXALGVLSPLVWXRIXGPPIEXPKXXPPEWISSYXNPGXSKK
*i*xikskkkkididk*kxflv
Translated Amino Acid sequence (All Frames) 
Frame A:
ltt*riqvdtsllsqp*kkd*fkssqvklnklnn*klnmvtkslvpvllhklmvv*dqln
h*llkhhh*iqkkaldsvv*qsqnvkrnyqklqvvlnhyqkvfygyf*lvkfqlnhklkp
yq---

---YGLKXXNXYPVLFGXSXALGVLSPLVWXRIXGPPIEXPKXXPPEWISSYXNPGXSKK
*i*xikskkkkididk*kxflv

Frame B:
*llkefr*illy*anlkrkissnhpr*n*ts*tikn*tw*qnhwylycctslwwyeis*i
isy*niiirsrrrh*ipwfnnprmsreitkssrwc*titrryfmvtfnw*ssn*its*nl
i---

---ma*xnxtxtlfysxxqxplxfyxp*fgxgs*xpxskxxxxxplngfxptxtxaxqkn
kfkx*npkkkk*istskkxfw

Frame C:
NYLKNSGRYFSTEPTLKERLVQIIPGKIEQVKQLKTEHGDKIIGTCTVAQAYGGMRSVKS
LVTETSSLDPEEGIRFRGLTIPECQEKLPKAPGGAEPLPEGILWLLLTGEVPTESQVKTL
S---

---wlkxtxlxpcfirxfxspwxfixlslgsdxrxpxrkxxxxpp*mdfxlrkpxpikki
nlxnkiqkkknryrqvknxfg
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ738 (VSJ738Q) /CSM/VS/VSJ7-B/VSJ738Q.Seq.d/                        726   0.0
VFO894 (VFO894Q) /CSM/VF/VFO8-D/VFO894Q.Seq.d/                        726   0.0
VFO812 (VFO812Q) /CSM/VF/VFO8-A/VFO812Q.Seq.d/                        726   0.0
VFO680 (VFO680Q) /CSM/VF/VFO6-D/VFO680Q.Seq.d/                        726   0.0
VFO296 (VFO296Q) /CSM/VF/VFO2-D/VFO296Q.Seq.d/                        726   0.0
VFO156 (VFO156Q) /CSM/VF/VFO1-C/VFO156Q.Seq.d/                        726   0.0
VFO142 (VFO142Q) /CSM/VF/VFO1-B/VFO142Q.Seq.d/                        726   0.0
VFO108 (VFO108Q) /CSM/VF/VFO1-A/VFO108Q.Seq.d/                        726   0.0
VFN772 (VFN772Q) /CSM/VF/VFN7-C/VFN772Q.Seq.d/                        726   0.0
VFN189 (VFN189Q) /CSM/VF/VFN1-D/VFN189Q.Seq.d/                        726   0.0
own update 2003.12. 6
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   424  0.0  6
AY126274|AY126274.1 Candida krusei citrate synthase (CS1) gene, complete cds.    54  1e-10  3
AX536976|AX536976.1 Sequence 577 from Patent WO02064766.          76  6e-10  1
AL429256|AL429256.1 clone BA0AB030F08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    64  1e-09  2
AY145050|AY145050.1 Kluyveromyces lactis clone P2263 CIT1 gene, partial cds.    64  2e-09  2
CD450751|CD450751.1 USDA-FP_102714 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-37_E02 5', mRNA sequence.    46  0.001  2
BE579168|BE579168.1 kq23d01.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T20G5.2 CE00513 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    34  0.001  3
CB832670|CB832670.1 USDA-FP_100461 Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clone WHWTC-07_A07 5', mRNA sequence.    46  0.002  2
BE581402|BE581402.1 kq45d02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to WP:T20G5.2 CE00513 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    34  0.023  3
CD526170|CD526170.1 kw31d09.y1 Strongyloides ratti PA female naive pAMP1 v1 Strongyloides ratti cDNA 5' similar to SW:CISY_CAEEL P34575 PROBABLE CITRATE SYNTHASE, MITOCHONDRIAL PRECURSOR ;, mRNA sequence.    46  0.026  2
dna update 2003. 7.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q553V1) RecName: Full=Citrate synthase, mitochondrial;         ...   244   1e-63
AC116305_8(AC116305|pid:none) Dictyostelium discoideum chromosom...   243   3e-63
FM992695_315(FM992695|pid:none) Candida dubliniensis CD36 chromo...   157   3e-37
(P79024) RecName: Full=Citrate synthase, mitochondrial;         ...   155   1e-36
AJ296102_1(AJ296102|pid:none) Podospora anserina cit1 gene for m...   152   7e-36
CP000502_263(CP000502|pid:none) Pichia stipitis CBS 6054 chromos...   151   1e-35
AF193854_1(AF193854|pid:none) Saccharomyces kluyveri putative ci...   150   2e-35
AJ243204_1(AJ243204|pid:none) Aspergilluse niger citA gene for c...   150   3e-35
CR382131_112(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   149   4e-35
AY144810_1(AY144810|pid:none) Saccharomyces bayanus clone Contig...   149   4e-35
protein update 2009. 3.25
PSORT 

 psg: 0.76  gvh: 0.49  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFA144 is cyt
5' end seq. ID AFA144F
5' end seq. 
>AFA144F.Seq
CTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGCCAACCTTAAAAGAAAGAT
TAGTTCAAATCATCCCAGGTAAAATTGAACAAGTTAAACAATTAAAAACTGAACATGGTG
ACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTGGTATGAGATCAGTTAAAT
CATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCATTAGATTCCGTGGTTTAA
CAATCCCAGAATGTCAAGAGAAATTACCAAAAGCTCCAGGTGGTGCTGAACCATTACCAG
AAGGTATTTTATGGTTACTTTTAACTGGTGAAGTTCCAACTGAATCACAAGTTAAAACCT
TATCAA----------
Length of 5' end seq. 366
3' end seq. ID AFA144Z
3' end seq. 
>AFA144Z.Seq
----------TATGGCTTAAAANAACANAACTTNTACCCTGTTTTATTCGGNGNTTCAAN
AGCCCTTGGNGTTTTATCNCCCTTAGTTTGGGNTCGGATCNTAGGNCCCCCNATCGAAAG
NCCNAAANCNGNCCCCCCTGAATGGATTTCNTCCTACGNAAACCCNGGCCNATCAAAAAA
ATAAATTTAAGNAATAAAATCCAAAAAAAAAAAAATAGATATCGACAAGTAAAAAATNTT
TTTGGTT
Length of 3' end seq. 237
Connected seq. ID AFA144P
Connected seq. 
>AFA144P.Seq
CTAACTACTTAAAGAATTCAGGTAGATACTTCTCTACTGAGCCAACCTTAAAAGAAAGAT
TAGTTCAAATCATCCCAGGTAAAATTGAACAAGTTAAACAATTAAAAACTGAACATGGTG
ACAAAATCATTGGTACCTGTACTGTTGCACAAGCTTATGGTGGTATGAGATCAGTTAAAT
CATTAGTTACTGAAACATCATCATTAGATCCAGAAGAAGGCATTAGATTCCGTGGTTTAA
CAATCCCAGAATGTCAAGAGAAATTACCAAAAGCTCCAGGTGGTGCTGAACCATTACCAG
AAGGTATTTTATGGTTACTTTTAACTGGTGAAGTTCCAACTGAATCACAAGTTAAAACCT
TATCAA----------TATGGCTTAAAANAACANAACTTNTACCCTGTTTTATTCGGNGN
TTCAANAGCCCTTGGNGTTTTATCNCCCTTAGTTTGGGNTCGGATCNTAGGNCCCCCNAT
CGAAAGNCCNAAANCNGNCCCCCCTGAATGGATTTCNTCCTACGNAAACCCNGGCCNATC
AAAAAAATAAATTTAAGNAATAAAATCCAAAAAAAAAAAAATAGATATCGACAAGTAAAA
AATNTTTTTGGTT
Length of connected seq. 603
Full length Seq ID -
Full length Seq. -
Length of full length seq. -