>AFF412 (AFF412Q) /CSM/AF/AFF4-A/AFF412Q.Seq.d/
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTCCATCATTGGTAGTTTGCTTAGATAGTGGTXXXXXXXXXXGATCGTGCTGAAAAT
CAATTAACTGGTGAAGTCGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGAT
CAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTAC
AAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTAT
CCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTC
ATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCT
GATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTT
ACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTA
GAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGT
GAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATC
GTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATGAATTCTTAGATATT
CCATTTGCCAAGAAATTATTAAGTTGTGTTATTTATTTATTAGATGCTCATTCCAAGTTN
ATTAAATAAATAAACCTTNTTTCA

FLFYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQIPSLVVCLDSG---

---DRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQ
LMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFET
NPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLH
DRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLSCVIYLLDAHSKXIK*inlxs

Frame A:
ifyfifqlikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmv
iqkkqfny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnh
hslmhgmkdyihtkqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkv
pknlvqsiyhnmlrssklkfkfhhw*fa*iv---

---DRAENQLTGEVDKRLHVEIPQHRLDQIAKCAEILGETIYDEFSWYKNTTPVTKDLTQ
LMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKVSVRLPPSFKAADASTVLKEIFET
NPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEASNLFFGKDHVYMGEGGSIPFMKMLH
DRFNCQFIVTGLLGPGSNAHGPNEFLDIPFAKKLLSCVIYLLDAHSKXIK*inlxs

Frame B:
ffilfsn**n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwl
yrrsnsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*ttt
th*cmg*ritsiqsshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rf
rriwfnpfttic*evps*nsnsiigsllr*w---

---ivlkin*lvksikdytlkfhniv*iksqnvlkf*akpsmmnshgtkiplqslki*pn
**stklgiqlfaspvlkvfhtlqmlvms*eltpplklvfvyhhhskqlmhqly*kkfskl
ihhmvlklpskwinvlqvgihhkldqn*kkh*eklqifslakttytwvkvvpshs*kcym
tvsivnlsllvy*vqvqmlmvqmns*ifhlprny*vvlfiy*mlipsxlnk*txf

Frame C:
FLFYFPIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGY
TEEAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPP
LTDAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGS
EESGSIHLPQYVEKFQAEIQIPSLVVCLDSG---

---sc*ksinw*sr*kitr*nsttsfrsnrkmc*nfrrnhl**ilmvqkyhssh*rfnpi
ddqqnlvsnylhhrc*rysthfkcw*chenshhr*s*csfttiiqss*cincikrnfrn*
stiwc*sylqng*mcfrlgfttn*trirksirrsfksflwqrprihg*rwfhpihenvt*
pfqlsiyrywfirsrfkcswsk*ilrysicqeiiklcylfircsfqvx*inkpxf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF412 (AFF412Q) /CSM/AF/AFF4-A/AFF412Q.Seq.d/                       2442   0.0
AFO568 (AFO568Q) /CSM/AF/AFO5-C/AFO568Q.Seq.d/                       1374   0.0
AFN766 (AFN766Q) /CSM/AF/AFN7-C/AFN766Q.Seq.d/                       1374   0.0
AFL825 (AFL825Q) /CSM/AF/AFL8-B/AFL825Q.Seq.d/                       1374   0.0
AFK591 (AFK591Q) /CSM/AF/AFK5-D/AFK591Q.Seq.d/                       1374   0.0
AFH219 (AFH219Q) /CSM/AF/AFH2-A/AFH219Q.Seq.d/                       1374   0.0
AFG485 (AFG485Q) /CSM/AF/AFG4-D/AFG485Q.Seq.d/                       1374   0.0
AFD440 (AFD440Q) /CSM/AF/AFD4-B/AFD440Q.Seq.d/                       1374   0.0
AFD120 (AFD120Q) /CSM/AF/AFD1-A/AFD120Q.Seq.d/                       1374   0.0
VFI168 (VFI168Q) /CSM/VF/VFI1-C/VFI168Q.Seq.d/                       1368   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC024357|AC024357.3 Mus musculus clone RP23-1E4, WORKING DRAFT SEQUENCE, 41 unordered pieces.    34   0.012  12
AL354810|AL354810.8 Human DNA sequence from clone RP11-527N12 on chromosome 13.    38   0.040  11
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34   0.059  18
AC121570|AC121570.4 Mus musculus BAC clone RP23-228G6 from 16, complete sequence.    38   0.089  8
AC116446|AC116446.4 Mus musculus chromosome 9 clone RP24-142M20 map 9, WORKING DRAFT SEQUENCE, 5 ordered pieces.    36   0.15   9
AC011911|AC011911.4 Drosophila melanogaster 3L BAC RP98-48J5 (Roswell Park Cancer Institute Drosophila BAC Library) complete sequence.    36   0.22   8
CG730970|CG730970.1 1119131F03.y1 1119 - RescueMu Grid AA Zea mays genomic, genomic survey sequence.    28   0.22   4
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    34   0.23   13
CK266014|CK266014.1 EST712092 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POAC219 3' end, mRNA sequence.    48   0.33   1
CK261663|CK261663.1 EST707741 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABB34 3' end, mRNA sequence.    48   0.33   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   213   9e-54
AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   212   2e-53
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   211   4e-53
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   211   6e-53
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   211   6e-53
AM039952_3097(AM039952|pid:none) Xanthomonas campestris pv. vesi...   209   1e-52
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   208   3e-52
DQ384331_1(DQ384331|pid:none) Physarum polycephalum succinyl-dia...   206   1e-51
AE016828_97(AE016828|pid:none) Coxiella burnetii RSA 493, comple...   196   2e-48
CP001020_1727(CP001020|pid:none) Coxiella burnetii CbuK_Q154, co...   196   2e-48

 psg: 0.70  gvh: 0.29  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for AFF412 is cyt

>AFF412F.Seq
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTCCATCATTGGTAGTTTGCTTAGATAGTGGT----------

>AFF412Z.Seq
----------GATCGTGCTGAAAATCAATTAACTGGTGAAGTCGATAAAAGATTACACGT
TGAAATTCCACAACATCGTTTAGATCAAATCGCAAAATGTGCTGAAATTTTAGGCGAAAC
CATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAGATTTAACCCA
ATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTGAAGGTATTCC
ACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAGTTAGTGTTCG
TTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAATTTTCGAAAC
TAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGA
TTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTT
TGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGAAAATGTTACA
TGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCA
TGGTCCAAATGAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTGTGTTATTTA
TTTATTAGATGCTCATTCCAAGTTNATTAAATAAATAAACCTTNTTTCA

>AFF412P.Seq
ATTTTTTATTTTATTTTCCAATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTG
AAAAAGTTAAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAA
AATATATTGAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTT
ATACAGAAGAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTA
TTTCATGTGAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAG
CAACCAAAACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCAC
CACTCACTGATGCATGGGATGAAGGATTACATCCATACAAAGCAGTCATCAAGAATAACA
AACTCTATGGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAA
TCAAAGCATTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTT
CCGAAGAATCTGGTTCAATCCATTTACCACAATATGTTGAGAAGTTCCAAGCTGAAATTC
AAATTCCATCATTGGTAGTTTGCTTAGATAGTGGT----------GATCGTGCTGAAAAT
CAATTAACTGGTGAAGTCGATAAAAGATTACACGTTGAAATTCCACAACATCGTTTAGAT
CAAATCGCAAAATGTGCTGAAATTTTAGGCGAAACCATCTATGATGAATTCTCATGGTAC
AAAAATACCACTCCAGTCACTAAAGATTTAACCCAATTGATGATCAACAAAACTTGGTAT
CCAACTATTTGCATCACCGGTGCTGAAGGTATTCCACACACTTCAAATGCTGGTAATGTC
ATGAGAACTCACACCACCGTTAAAGTTAGTGTTCGTTTACCACCATCATTCAAAGCAGCT
GATGCATCAACTGTATTAAAAGAAATTTTCGAAACTAATCCACCATATGGTGCTGAAGTT
ACCTTCAAAATGGATAAATGTGCTTCAGGTTGGGATTCACCACAAACTAGACCAGAATTA
GAAAAAGCATTAGGAGAAGCTTCAAATCTTTTCTTTGGCAAAGACCACGTATACATGGGT
GAAGGTGGTTCCATCCCATTCATGAAAATGTTACATGACCGTTTCAATTGTCAATTTATC
GTTACTGGTTTATTAGGTCCAGGTTCAAATGCTCATGGTCCAAATGAATTCTTAGATATT
CCATTTGCCAAGAAATTATTAAGTTGTGTTATTTATTTATTAGATGCTCATTCCAAGTTN
ATTAAATAAATAAACCTTNTTTCA