>CHC218 (CHC218Q) /CSM/CH/CHC2-A/CHC218Q.Seq.d/
ACTGTTGGCCTACTGGAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTATTTCTC
GTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAAATCAG
GAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAG
CATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTATCAAAG
TTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACCAAGAT
CATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAAGGAAG
TACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAATTGTG
CAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATCAATGA
GTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCCAATCA
TTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGAXXXXXXXXXXAAATTCACACAAG
AAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTA
AAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACC
ACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACTACCGCT
GGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACC
GCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTC
GAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCT
GGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCA
TTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGT
ATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGA
GAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTAT

CWPTGTNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKRGA
FKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPKEV
PCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDPII
SGCYNTMGQTS---

---NSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIREKTTVADLAKLKPAFK
EGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAAVGVEPSIMGIGPAVAI
PAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPNGSGIALGHPLGATGAR
QMATISRELHRRKGKYGVISMC

Frame A:
tvgllelikchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqfvklkee
hskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*qvyqrk
yhviqstdnvhqvykqlpivqqqskqvsmtlv*qav*nq*v*iqwhgmvvstrlpkriqs
lvvvttlwvkpl---

---kftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rrryp*enhrcrfgqiktsfq
rgwynyrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrsfcccrcrtihygywssrcn
ssrlktswshh*ryrrf*nq*sfcfssilql*gfgfgyeqs*skwlwyrfrssircywcp
sngyhfkritss*r*iwcnlyvy

Frame B:
llaywn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl*s*krs
iqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiisrftkgs
tmlysqqtmfirftsncqlcsssqsrfl*hwfsrrcrinefksngmgwwfqrgcprgsnh
*wllqhygsnl*---

---NSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDEGIREKTTVADLAKLKPAFK
EGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVAFAAVGVEPSIMGIGPAVAI
PAALKQAGLTTKDIDVFEINEAFASQAFYSCKVLGLDMNKVNPNGSGIALGHPLGATGAR
QMATISRELHRRKGKYGVISMC

Frame C:
CWPTGTNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAICKAKRGA
FKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLAGLPKEV
PCYTVNRQCSSGLQAIANCAAAVKAGFYDIGLAGGVESMSLNPMAWDGGFNEVAQEDPII
SGCYNTMGQTS---

---ihtrrqvppkkqvnltmksfqsp*rprmakpsssiktkvsvrkpplqiwpn*nqlsk
rvvqlplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*llllsvsnhplwvlvqplqf
qpp*nklvsplkistflksmkllllkhftvvrfwvwi*tkliqmalvsl*vih*vllvpv
kwlpfqenyivvkvnmv*slcv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHC218 (CHC218Q) /CSM/CH/CHC2-A/CHC218Q.Seq.d/                       2294   0.0
VFM101 (VFM101Q) /CSM/VF/VFM1-A/VFM101Q.Seq.d/                       1181   0.0
VFL724 (VFL724Q) /CSM/VF/VFL7-A/VFL724Q.Seq.d/                       1181   0.0
VFK696 (VFK696Q) /CSM/VF/VFK6-D/VFK696Q.Seq.d/                       1181   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1181   0.0
SSJ636 (SSJ636Q) /CSM/SS/SSJ6-B/SSJ636Q.Seq.d/                       1181   0.0
SLH575 (SLH575Q) /CSM/SL/SLH5-D/SLH575Q.Seq.d/                       1181   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1181   0.0
SLG603 (SLG603Q) /CSM/SL/SLG6-A/SLG603Q.Seq.d/                       1181   0.0
SLE133 (SLE133Q) /CSM/SL/SLE1-B/SLE133Q.Seq.d/                       1181   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1181   0.0    9
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    40   1e-14  6
CO090724|CO090724.1 GR__Ea10N15.f GR__Ea Gossypium raimondii cDNA clone GR__Ea10N15 5', mRNA sequence.    48   2e-12  4
CO113715|CO113715.1 GR__Eb014D02.r GR__Eb Gossypium raimondii cDNA clone GR__Eb014D02 3', mRNA sequence.    48   2e-12  4
CA858588|CA858588.1 EST635843 GLSD Medicago truncatula cDNA clone pGLSD-38M16, mRNA sequence.    40   9e-11  5
CO088271|CO088271.1 GR__Ea07C11.f GR__Ea Gossypium raimondii cDNA clone GR__Ea07C11 5', mRNA sequence.    44   4e-10  4
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   7e-10  4
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    40   5e-09  4
CO085238|CO085238.1 GR__Ea02E14.r GR__Ea Gossypium raimondii cDNA clone GR__Ea02E14 3', mRNA sequence.    48   1e-08  3
CN604220|CN604220.1 USDA_FP_131320 Vitis shuttleworthii L., grape Vitis shuttleworthii cDNA clone WHVs010_C03 5', mRNA sequence.    60   3e-08  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   365   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   215   e-102
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   214   e-102
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   213   e-102
(Q56WD9) RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal;  ...   217   e-100
T07989(T07989) acetyl-CoA C-acyltransferase (EC 2.3.1.16) precur...   216   e-100
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   222   e-100
BT034496_1(BT034496|pid:none) Zea mays full-length cDNA clone ZM...   222   e-100
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   211   e-100
AX025481_1(AX025481|pid:none) Sequence 7 from Patent WO0032789.       212   e-100

 psg: 0.61  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.36  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for CHC218 is nuc

>CHC218F.Seq
ACTGTTGGCCTACTGGAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTATTTCTC
GTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAAATCAG
GAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAG
CATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTATCAAAG
TTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACCAAGAT
CATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAAGGAAG
TACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAATTGTG
CAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATCAATGA
GTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCCAATCA
TTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGANNNNNNNNNN

>CHC218Z.Seq
NNNNNNNNNNAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACG
ATGAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAG
ACGAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCA
AAGAGGGTGGTACAACTACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTT
GTTTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCG
TAGCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAA
TTCCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCA
ATGAAGCTTTTGCTTCTCAAGCATTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACA
AAGTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCC
GTCAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCT
CTATGTGTAT

>CHC218P.Seq
ACTGTTGGCCTACTGGAACTAATAAAATGTCATTCAATTCAAGAATTGAAACTATTTCTC
GTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTTCTGGATCAGTTAAATCAG
GAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTTGTAAAGCTAAAAGAGGAG
CATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTATTAAACATATTATCAAAG
TTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGGGAGCAGTATTACCAAGAT
CATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAGCAGGTTTACCAAAGGAAG
TACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTACAAGCAATTGCCAATTGTG
CAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAGCAGGCGGTGTAGAATCAATGA
GTTTAAATCCAATGGCATGGGATGGTGGTTTCAACGAGGTTGCCCAAGAGGATCCAATCA
TTAGTGGTTGTTACAACACTATGGGTCAAACCTCTGA----------AAATTCACACAAG
AAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAATCGTTCCAGTCACCGTA
AAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAGGTATCCGTGAGAAAACC
ACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGGGTGGTACAACTACCGCT
GGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAGTTATGAAAAGAAGTACC
GCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTTTTGCTGCTGTCGGTGTC
GAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAGCCGCCTTAAAACAAGCT
GGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAGCTTTTGCTTCTCAAGCA
TTTTACAGTTGTAAGGTTTTGGGTTTGGATATGAACAAAGTTAATCCAAATGGCTCTGGT
ATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAATGGCTACCATTTCAAGA
GAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGTGTAT