>CHC190 (CHC190Q) /CSM/CH/CHC1-D/CHC190Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCAXXXXXXXX
XXAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAA
AGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGA
AGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGT
CAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAA
TCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGA
ATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTT
TAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAA
CAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGT
TGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGA
GAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGA
AGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTTANACAAAAAA
GAAGAAGTCAAAAAATCANCCAAAAAAGT

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLV---

---IQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPE
AVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTXTKKKKSKNXPKK

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLV---

---rskqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliq
klsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmns
tslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppk
lkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklxqkrrsqkixqks

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlws---

---dpsr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*sr
scqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tl
pl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs
*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*nlxkkeevkksxkk

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfg---

---IQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPE
AVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELY
LFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKA
EERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTXTKKKKSKNXPKK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHC190 (CHC190Q) /CSM/CH/CHC1-D/CHC190Q.Seq.d/                       1998   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1090   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1090   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1090   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1090   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       1090   0.0
SFH171 (SFH171Q) /CSM/SF/SFH1-C/SFH171Q.Seq.d/                       1090   0.0
SFG873 (SFG873Q) /CSM/SF/SFG8-D/SFG873Q.Seq.d/                       1090   0.0
CHC137 (CHC137Q) /CSM/CH/CHC1-B/CHC137Q.Seq.d/                       1090   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       1090   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1090   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   9e-28  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-10  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
BT013428|BT013428.1 Lycopersicon esculentum clone 132068F, mRNA sequence.    74   4e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   4e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   5e-09  3
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74   6e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74   6e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   332   e-180
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   210   e-103
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   206   e-103
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   207   e-103
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   202   e-103
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   200   e-103
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   205   e-103
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   205   e-102
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   204   e-102
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   199   e-101

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum

>> prediction for CHC190 is exc

>CHC190F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCANNNNNNNN
NN

>CHC190Z.Seq
NNNNNNNNNNAGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAA
CAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGAT
GACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCA
GAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACC
ATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATAC
AAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTC
TACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCC
ATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAG
ACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAA
GCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAA
GCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTTAN
ACAAAAAAGAAGAAGTCAAAAAATCANCCAAAAAAGT

>CHC190P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCA--------
--AGATCCAAGCAGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAA
AGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGA
AGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGT
CAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAA
TCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGA
ATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTT
TAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAA
CAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGT
TGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGA
GAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGA
AGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTTANACAAAAAA
GAAGAAGTCAAAAAATCANCCAAAAAAGT