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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF405
Library AF
(Link to library)
Clone ID AFF405
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16540-1
Original site URL
Representative seq. ID AFF405P
(Link to Original site)
Representative DNA sequence 
>AFF405 (AFF405Q) /CSM/AF/AFF4-A/AFF405Q.Seq.d/
AAATTAAATAAATAATAAAATAAAAAAATAAAAAAAATGAGGATAAATAATTTATTAGTA
TGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAATGCAAATCCGGAACATGATAAA
TGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCAGAGGACCAATGTAAAGCACCA
AATTCTGGTTGTAAGTATTTACCTTTTGTTTCATGTTGTGGTACAAAGAAATTCTTTTGT
GTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTAAGTTGTATGAAAGATTCAAAA
ACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCAAATTCACCATTTTTATATGAT
TATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGTGAAGCAAGTGGAGGAGTTTGT
GAATGGGTTGACCCAGTACCATGTATGGGTACATCATGTTGTCCACGTTATCCAGTTTGT
AAGGGTGGTGGTGGCGGTGGTGGACCAGTCTCACCATGTAAGAATGTAATTTGTCCAGAG
GATTATTGTTGTCAAGATATTCATGGTGGAGCTTACTGTGTTGAAAAGCCAAGACCACCA
CCACCAAAACCXXXXXXXXXXGAAAAGGAATGCGAGCATCACCATTGTCCACCAGGATAT
GATTGCGTGGTCGACTCTCACCATCGTCCACATTGCCAAAGACCAAATCCAGGCTCATTA
TGTCGTAATGTAACTTGTCCATATGGCTATGTTTGCAAGGCAATTAATAATCTTCCAACA
TGTATTCGTAATCCATTACCACCAAACTACCCATGTAGAGATTTACATTGCCCATCTGGA
TACTCTTGCGAAATCATCAATGATCTCCCATCATGCGTAAGAGAAACCCATCCAGGTCAT
TGTAAAACTTGTCATGATGTTAATTGTGGTTCATTGGATTGCGCAATGGTTCCTAATAAA
TGTCCAAGAGGTTCTCGTGACTGCTGTATTCAAATTCCATCGTGTCGTTTACCATCAAAT
TCTAATGATGATTATGATAATGATCCTAATGGATATGATGATAATGAAAATGAGGGTGAT
GGATTATCAATTCCATGTGGCCCAATTATCACTTGTAAATTAAATGAAATTTGTTTATTA
GAACAATCACGTTGTGCCCCATTATGTGAATTTGTAAAATGTTCACCAGGAACTAAATGT
GTTGCTGATACAACTGGAATTCCAGTTTGTTTACCAAATGGAATAACTCCATATTAAATT
TAAATAAAAAAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
klnk**NKKIKKMRINNLLVCLVLVFSTLSISNANPEHDKWWKPPPRNCDSLSEDQCKAP
NSGCKYLPFVSCCGTKKFFCVEDNGNGCGNAPLSCMKDSKTDAIYEIWSSCRPNSPFLYD
YQVRNETCDQKLCEASGGVCEWVDPVPCMGTSCCPRYPVCKGGGGGGGPVSPCKNVICPE
DYCCQDIHGGAYCVEKPRPPPPK---

---EKECEHHHCPPGYDCVVDSHHRPHCQRPNPGSLCRNVTCPYGYVCKAINNLPTCIRN
PLPPNYPCRDLHCPSGYSCEIINDLPSCVRETHPGHCKTCHDVNCGSLDCAMVPNKCPRG
SRDCCIQIPSCRLPSNSNDDYDNDPNGYDDNENEGDGLSIPCGPIITCKLNEICLLEQSR
CAPLCEFVKCSPGTKCVADTTGIPVCLPNGITPY*i*ikk
Translated Amino Acid sequence (All Frames) 
Frame A:
klnk**NKKIKKMRINNLLVCLVLVFSTLSISNANPEHDKWWKPPPRNCDSLSEDQCKAP
NSGCKYLPFVSCCGTKKFFCVEDNGNGCGNAPLSCMKDSKTDAIYEIWSSCRPNSPFLYD
YQVRNETCDQKLCEASGGVCEWVDPVPCMGTSCCPRYPVCKGGGGGGGPVSPCKNVICPE
DYCCQDIHGGAYCVEKPRPPPPK---

---EKECEHHHCPPGYDCVVDSHHRPHCQRPNPGSLCRNVTCPYGYVCKAINNLPTCIRN
PLPPNYPCRDLHCPSGYSCEIINDLPSCVRETHPGHCKTCHDVNCGSLDCAMVPNKCPRG
SRDCCIQIPSCRLPSNSNDDYDNDPNGYDDNENEGDGLSIPCGPIITCKLNEICLLEQSR
CAPLCEFVKCSPGTKCVADTTGIPVCLPNGITPY*i*ikk

Frame B:
n*innkikk*kk*g*iiy*yvwf*fsqhyqlvmqirnminggnllleivivcqrtnvkhq
ilvvsiyllfhvvvqrnsfv*kimemvvemlh*vv*kiqkpmqfmrfgvhvdqihhfymi
iklemklviksyvkqveefvngltqyhvwvhhvvhviqfvrvvvavvdqshhvrm*fvqr
iivvkifmveltvlksqdhhhqn---

---krnasitivhqdmiawstltivhiakdqiqahyvvm*lvhmamfarqliifqhvfvi
hyhqtthveiyiahldtlakssmishha*ekpiqvivklvmmlivvhwiaqwflinvqev
lvtavfkfhrvvyhqilmmimimilmdmmimkmrvmdyqfhvaqlslvn*mkfvy*nnhv
vphyvnl*nvhqelnvlliqlefqfvyqme*lhikfk*kk

Frame C:
ik*iik*knkknedk*fismfgfsflniin**cksgt**mvetss*kl**fvrgpm*stk
fwl*vftfcfmlwykeillcrr*wkwlwkcsiklyerfknrcnl*dlefm*tkftifi*l
ss*k*nl*skam*skwrsl*mg*pstmygyimlstlssl*gwwwrwwtsltm*ecnlsrg
lllsryswwsllc*kakttttkt---

---kgmrasplstri*lrgrlspsstlpktksrlims*cnlsiwlclqgn**ssnmys*s
ittklpm*rftlpiwillrnhq*spimrkrnpsrsl*nls*c*lwfiglrngs**mskrf
s*llysnsivsftikf***l***s*wi****k*g*wiinsmwpnyhl*ik*nlfirtitl
cpim*ickmftrn*mcc*ynwnsslftkwnnsilnlnkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF405 (AFF405Q) /CSM/AF/AFF4-A/AFF405Q.Seq.d/                       1897   0.0
AFK345 (AFK345Q) /CSM/AF/AFK3-B/AFK345Q.Seq.d/                       1885   0.0
SLE226 (SLE226Q) /CSM/SL/SLE2-B/SLE226Q.Seq.d/                       1138   0.0
CFB214 (CFB214Q) /CSM/CF/CFB2-A/CFB214Q.Seq.d/                       1138   0.0
AFO864 (AFO864Q) /CSM/AF/AFO8-C/AFO864Q.Seq.d/                       1138   0.0
AFO193 (AFO193Q) /CSM/AF/AFO1-D/AFO193Q.Seq.d/                       1138   0.0
AFO188 (AFO188Q) /CSM/AF/AFO1-D/AFO188Q.Seq.d/                       1138   0.0
AFN157 (AFN157Q) /CSM/AF/AFN1-C/AFN157Q.Seq.d/                       1138   0.0
AFH222 (AFH222Q) /CSM/AF/AFH2-A/AFH222Q.Seq.d/                       1138   0.0
AFF732 (AFF732Q) /CSM/AF/AFF7-B/AFF732Q.Seq.d/                       1138   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC091776|AC091776.19 Chlamydomonas reinhardtii clone cr-4i21, complete sequence.    42   0.012  3
AC111035|AC111035.8 Mus musculus chromosome 3, clone RP24-507N18, complete sequence.    40   0.013  7
AP006671|AP006671.1 Lotus corniculatus var. japonicus genomic DNA, chromosome 2, clone:LjT39I14, TM0368c.    46   0.086  3
CG455185|CG455185.1 PUIJW02TBB ZM_0.6_1.0_KB Zea mays genomic clone ZMMBTa0600A03, genomic survey sequence.    34   0.21   3
CC262402|CC262402.1 CH261-72I18_Sp6.1 CH261 Gallus gallus genomic clone CH261-72I18, DNA sequence.    48   0.31   1
AC122735|AC122735.4 Mus musculus clone RP23-215I1, WORKING DRAFT SEQUENCE, 20 unordered pieces.    34   0.32   8
AC117072|AC117072.3 Dictyostelium discoideum chromosome 2 map 3879572-4071762 strain AX4, complete sequence.    32   0.35   10
AC087142|AC087142.1 Mus musculus clone RP23-211L19, WORKING DRAFT SEQUENCE, 65 unordered pieces.    38   0.40   8
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    32   0.41   20
AC019964|AC019964.1 Drosophila melanogaster, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    32   0.57   5
dna update 2004. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...   728   0.0
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       202   3e-50
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   198   3e-49
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   112   3e-23
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...   109   2e-22
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...   107   1e-21
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    71   9e-11
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    66   3e-09
(Q6TU45) RecName: Full=Probable spore coat protein sigD; AltName...    61   9e-08
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    56   2e-06
protein update 2009. 4.20
PSORT 

 psg: 0.98  gvh: 0.81  alm: 0.40  top: 0.17  tms: 0.00  mit: 0.37  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: extracellular, including cell wall
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for AFF405 is exc
5' end seq. ID AFF405F
5' end seq. 
>AFF405F.Seq
AAATTAAATAAATAATAAAATAAAAAAATAAAAAAAATGAGGATAAATAATTTATTAGTA
TGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAATGCAAATCCGGAACATGATAAA
TGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCAGAGGACCAATGTAAAGCACCA
AATTCTGGTTGTAAGTATTTACCTTTTGTTTCATGTTGTGGTACAAAGAAATTCTTTTGT
GTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTAAGTTGTATGAAAGATTCAAAA
ACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCAAATTCACCATTTTTATATGAT
TATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGTGAAGCAAGTGGAGGAGTTTGT
GAATGGGTTGACCCAGTACCATGTATGGGTACATCATGTTGTCCACGTTATCCAGTTTGT
AAGGGTGGTGGTGGCGGTGGTGGACCAGTCTCACCATGTAAGAATGTAATTTGTCCAGAG
GATTATTGTTGTCAAGATATTCATGGTGGAGCTTACTGTGTTGAAAAGCCAAGACCACCA
CCACCAAAACC----------
Length of 5' end seq. 611
3' end seq. ID AFF405Z
3' end seq. 
>AFF405Z.Seq
----------GAAAAGGAATGCGAGCATCACCATTGTCCACCAGGATATGATTGCGTGGT
CGACTCTCACCATCGTCCACATTGCCAAAGACCAAATCCAGGCTCATTATGTCGTAATGT
AACTTGTCCATATGGCTATGTTTGCAAGGCAATTAATAATCTTCCAACATGTATTCGTAA
TCCATTACCACCAAACTACCCATGTAGAGATTTACATTGCCCATCTGGATACTCTTGCGA
AATCATCAATGATCTCCCATCATGCGTAAGAGAAACCCATCCAGGTCATTGTAAAACTTG
TCATGATGTTAATTGTGGTTCATTGGATTGCGCAATGGTTCCTAATAAATGTCCAAGAGG
TTCTCGTGACTGCTGTATTCAAATTCCATCGTGTCGTTTACCATCAAATTCTAATGATGA
TTATGATAATGATCCTAATGGATATGATGATAATGAAAATGAGGGTGATGGATTATCAAT
TCCATGTGGCCCAATTATCACTTGTAAATTAAATGAAATTTGTTTATTAGAACAATCACG
TTGTGCCCCATTATGTGAATTTGTAAAATGTTCACCAGGAACTAAATGTGTTGCTGATAC
AACTGGAATTCCAGTTTGTTTACCAAATGGAATAACTCCATATTAAATTTAAATAAAAAA
AA
Length of 3' end seq. 652
Connected seq. ID AFF405P
Connected seq. 
>AFF405P.Seq
AAATTAAATAAATAATAAAATAAAAAAATAAAAAAAATGAGGATAAATAATTTATTAGTA
TGTTTGGTTTTAGTTTTCTCAACATTATCAATTAGTAATGCAAATCCGGAACATGATAAA
TGGTGGAAACCTCCTCCTAGAAATTGTGATAGTTTGTCAGAGGACCAATGTAAAGCACCA
AATTCTGGTTGTAAGTATTTACCTTTTGTTTCATGTTGTGGTACAAAGAAATTCTTTTGT
GTAGAAGATAATGGAAATGGTTGTGGAAATGCTCCATTAAGTTGTATGAAAGATTCAAAA
ACCGATGCAATTTATGAGATTTGGAGTTCATGTAGACCAAATTCACCATTTTTATATGAT
TATCAAGTTAGAAATGAAACTTGTGATCAAAAGCTATGTGAAGCAAGTGGAGGAGTTTGT
GAATGGGTTGACCCAGTACCATGTATGGGTACATCATGTTGTCCACGTTATCCAGTTTGT
AAGGGTGGTGGTGGCGGTGGTGGACCAGTCTCACCATGTAAGAATGTAATTTGTCCAGAG
GATTATTGTTGTCAAGATATTCATGGTGGAGCTTACTGTGTTGAAAAGCCAAGACCACCA
CCACCAAAACC----------GAAAAGGAATGCGAGCATCACCATTGTCCACCAGGATAT
GATTGCGTGGTCGACTCTCACCATCGTCCACATTGCCAAAGACCAAATCCAGGCTCATTA
TGTCGTAATGTAACTTGTCCATATGGCTATGTTTGCAAGGCAATTAATAATCTTCCAACA
TGTATTCGTAATCCATTACCACCAAACTACCCATGTAGAGATTTACATTGCCCATCTGGA
TACTCTTGCGAAATCATCAATGATCTCCCATCATGCGTAAGAGAAACCCATCCAGGTCAT
TGTAAAACTTGTCATGATGTTAATTGTGGTTCATTGGATTGCGCAATGGTTCCTAATAAA
TGTCCAAGAGGTTCTCGTGACTGCTGTATTCAAATTCCATCGTGTCGTTTACCATCAAAT
TCTAATGATGATTATGATAATGATCCTAATGGATATGATGATAATGAAAATGAGGGTGAT
GGATTATCAATTCCATGTGGCCCAATTATCACTTGTAAATTAAATGAAATTTGTTTATTA
GAACAATCACGTTGTGCCCCATTATGTGAATTTGTAAAATGTTCACCAGGAACTAAATGT
GTTGCTGATACAACTGGAATTCCAGTTTGTTTACCAAATGGAATAACTCCATATTAAATT
TAAATAAAAAAAA
Length of connected seq. 1263
Full length Seq ID -
Full length Seq. -
Length of full length seq. -