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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHC156
Library CH
(Link to library)
Clone ID CHC156
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13873-1
Original site URL
Representative seq. ID CHC156P
(Link to Original site)
Representative DNA sequence 
>CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/
ATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTAGAGG
TAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAGTACC
AAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAAGTCG
TTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCACCAAC
ATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAATTGGA
TGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGGCAGT
GGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAATTGGC
TGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAAAACA
TGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAAAATT
TAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCAXXXXXXXXXXTGCTATCAATCATT
CGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATC
CAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTA
GTTTAAATAGTGGTGAGCAATTAATGTNTAGATATTTAGAGATTTTAAAAAGTATCCAAT
TGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGC
TGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAG
AATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAAT
CGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTG
TTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGG
TCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATA
TAATAGATTAATGAAAATTGAAGAAGAACTTGAAAAACTTCATAGATCAAATGGTAATTC
ATTTGAGTATGCTGGTGAAGAGTTTTATCATTTTTAATAAAAATTAAAAAAAAATTAAAA
AATAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDGDKSR
YFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMAV
ARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIGAPKF
KEALRYGSEVY---

---AINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMXRYL
EILKSIQLHSLKIHLVKMIGKIMVKLQLQLVIKFKSLVMIYFVQIQKELKKQFLRKL*ih
yy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvhlv
dlkdyqniid**klkknlknfidqmvihlsmlvksfiifnkn*kkikk*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
iilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*ikrw**ks
lfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqcnrwcings
gkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr**ttisrvyasthwctki
*rsialwfrslp---

---cyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyriltfev*fk*w*ainv*if
rdfkkypiaffedpfgeddwenhgkitaaignkiqiigddllctnperikkaisektvns
lllkinqigtltetieaaklakeagwgclvshrsgetddhfiadlvvglgtgeiksgapc
rferlskynrlmkieeeleklhrsngnsfeyageefyhf**klkkn*kikk

Frame B:
*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFELRDGDKSR
YFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAIVGVSMAV
ARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGNRLPFQEFMLVPIGAPKF
KEALRYGSEVY---

---AINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMXRYL
EILKSIQLHSLKIHLVKMIGKIMVKLQLQLVIKFKSLVMIYFVQIQKELKKQFLRKL*ih
yy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvhlv
dlkdyqniid**klkknlknfidqmvihlsmlvksfiifnkn*kkikk*kk

Frame C:
nfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*emvikvv
ildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqslvyqwqw
qeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvidyhfkslc*yplvhqnl
kkhcvmvqkst---

---lsiiraikmllklvlilllqnfgiqrlqnmiwisri*dphi*slv*ivvsn*cxdi*
rf*kvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enckfi
iienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwctl*
i*kiiki**inen*rrt*kts*ikw*fi*vcw*rvlsflikikkklknkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       2389   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1273   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1265   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1265   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1251   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1227   0.0
CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       1221   0.0
CFG491 (CFG491Q) /CSM/CF/CFG4-D/CFG491Q.Seq.d/                       1219   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1217   0.0
CHD848 (CHD848Q) /CSM/CH/CHD8-B/CHD848Q.Seq.d/                       1195   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   1e-26  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   8e-19  6
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   8e-19  6
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   8e-19  6
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   8e-19  6
BM162118|BM162118.1 EST564641 PyBS Plasmodium yoelii yoelii cDNA clone PYCKN41 5' end, mRNA sequence.    70   5e-18  3
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    70   6e-18  3
BM164058|BM164058.1 EST566581 PyBS Plasmodium yoelii yoelii cDNA clone PYCLP21 5' end, mRNA sequence.    70   8e-16  2
BM166362|BM166362.1 EST568885 PyBS Plasmodium yoelii yoelii cDNA clone PYCNG69 5' end, mRNA sequence.    70   1e-15  2
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   4e-15  4
dna update 2004. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q27727) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   218   e-116
AM910990_161(AM910990|pid:none) Plasmodium knowlesi strain H chr...   219   e-115
BC054169_1(BC054169|pid:none) Xenopus laevis enolase 1, alpha, m...   226   e-114
BC158432_1(BC158432|pid:none) Xenopus tropicalis enolase 1, (alp...   226   e-113
(Q9U615) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   220   e-113
BC061287_1(BC061287|pid:none) Xenopus tropicalis enolase 1, (alp...   224   e-112
Z68318_3(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   218   e-112
(Q27527) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   218   e-111
DQ497989_1(DQ497989|pid:none) Typhlonectes natans enolase B mRNA...   228   e-111
(Q7RA60) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   200   e-110
protein update 2009. 3.29
PSORT 

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for CHC156 is cyt
5' end seq. ID CHC156F
5' end seq. 
>CHC156F.Seq
ATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTAGAGG
TAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAGTACC
AAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAAGTCG
TTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCACCAAC
ATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAATTGGA
TGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGGCAGT
GGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAATTGGC
TGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAAAACA
TGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAAAATT
TAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCANNNNNNNNNN
Length of 5' end seq. 586
3' end seq. ID CHC156Z
3' end seq. 
>CHC156Z.Seq
NNNNNNNNNNTGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATTGATT
CTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAGAATA
TAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTNTAGATATT
TAGAGATTTTAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTG
GGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGTGATGA
TTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTC
ATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCAAAACT
TGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCA
TTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTG
TAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTTGAAAA
ACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGTGAAGAGTTTTATCATTTTTA
ATAAAAATTAAAAAAAAATTAAAAAATAAAAAAAA
Length of 3' end seq. 695
Connected seq. ID CHC156P
Connected seq. 
>CHC156P.Seq
ATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAGAGAAATTTTGGATAGTAGAGG
TAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGGAATGTTTTCAGCCAAAGTACC
AAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATTAAGAGATGGTGATAAAAGTCG
TTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGTAAATAATTTAATATCACCAAC
ATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGATGATAAAATGATTGAATTGGA
TGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAATCGTTGGTGTATCAATGGCAGT
GGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTACCGTTACATTTCACAATTGGC
TGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAATGTTATTAATGGTGGAAAACA
TGCAGGTAATAGACTACCATTTCAAGAGTTTATGCTAGTACCCATTGGTGCACCAAAATT
TAAAGAAGCATTGCGTTATGGTTCAGAAGTCTACCA----------TGCTATCAATCATT
CGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATC
CAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTA
GTTTAAATAGTGGTGAGCAATTAATGTNTAGATATTTAGAGATTTTAAAAAGTATCCAAT
TGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAGC
TGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAAG
AATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAAT
CGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTTG
TTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTGG
TCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAATA
TAATAGATTAATGAAAATTGAAGAAGAACTTGAAAAACTTCATAGATCAAATGGTAATTC
ATTTGAGTATGCTGGTGAAGAGTTTTATCATTTTTAATAAAAATTAAAAAAAAATTAAAA
AATAAAAAAAA
Length of connected seq. 1261
Full length Seq ID -
Full length Seq. -
Length of full length seq. -