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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF394
Library AF
(Link to library)
Clone ID AFF394
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15456-1
Original site URL
Representative seq. ID AFF394P
(Link to Original site)
Representative DNA sequence 
>AFF394 (AFF394Q) /CSM/AF/AFF3-D/AFF394Q.Seq.d/
AACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTTTTTATTAATCAATTCCACATGA
TTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTT
AGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAG
AGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAAT
TTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGC
CTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCC
AATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATA
TGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGT
CTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGC
XXXXXXXXXXTGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTT
TCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATT
CAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCT
CCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCG
ATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGG
AATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTT
GTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTG
CCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTA
GCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAA
CTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGAT
TTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTG
CTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAG
GTTAAATTTTTTTAATTAATTTAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
tkilahrfqqrffy*SIPHDSIKGKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRR
VPQKWSNRLSALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCP
ITVVACDLHNKYNSGASSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAE-
--

---VKDQLFAEATAEPGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFS
FWLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTC
HAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLT
PKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG
*iflinl
Translated Amino Acid sequence (All Frames) 
Frame A:
nkdfgaqistkifllinst*fnkgeneityfnfifsyycfsssfnstiklpssfkri*kk
sstkmvkqiicsqcwyhnpnfrf*rcsilwchyhwyprsslqssfrywfiqlvdsikems
nhcccm*ft*qi*qrclkhicrqrn*fhhpir*wcyvrfclsrfrhcwfinc*rsiir*s
---

---c*rsiir*shcrtrycfrfrqirwyfrscfpihlc*fnstsllqhvitrfsfintll
llviknsrcqrw*tlirfnr*hqihw*hylrpinqrnllgiryg*lcyrwsiswflwyyl
srnlrfryithcwsng*yycpq*kircchlkw*rcll*l*ryqhltkcyhhrcws*icfn
skrirfrsy*vrkd*mfewiygyrvkhgkfldpw*cfhlcllycirfw**tswfrnchsr
lnffn*f

Frame B:
tkilahrfqqrffy*SIPHDSIKGKMKLLILTLFLATIVLAQALTVPLNFHQASRESRRR
VPQKWSNRLSALNAGTTIPISDFEDAQYYGAITIGTPGQAFKVVFDTGSSNLWIPSKKCP
ITVVACDLHNKYNSGASSTYVANGTDFTIQYGSGAMSGFVSQDSVTVGSLTVKDQLFAE-
--

---VKDQLFAEATAEPGIAFDFAKFDGILGLAFQSISVNSIPPVFYNMLSQGLVSSTLFS
FWLSRTPGANGGELSFGSIDNTKYTGDITYVPLTNETYWEFVMDDFAIDGQSAGFCGTTC
HAICDSGTSLIAGPMADITALNEKLGAVILNGEGVFSDCSVINTLPNVTITVAGREFVLT
PKEYVLEVTEFGKTECLSGFMGIELNMGNFWILGDVFISAYYTVFDFGNKQVGFATAIQG
*iflinl

Frame C:
qrfwrtdfnkdffinqfhmiq*rgk*nylf*lyf*lllf*lkl*qyh*tsiklqenleee
fhkngqtdyllsmlvpqsqfqilkmlntmvplplvpqvkpsk*fsilvhptcgfhqrnvq
slllhviyitnitavpqahmsptelispsntvvvlcqvlslkipsllvh*llkinyslk-
--

---lkinyslkplpnqvllsispnsmvf*vllsnpslliqfhqsfttcyhkv*fhqhssp
sgyqelqvptvvnshsvqsitpntlvtlptsh*ptkpignslwmtllsmvnqlvsvvllv
tqfaiqvhhsllvqwlillpsmkn*vlss*mvkvsslivalstpyqmlpspllvvnlf*l
qkntf*kllsserlnv*vdlwvss*tweisgslvmfsslltilysilvinklvsqlpfkv
kff*liy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN380 (VFN380Q) /CSM/VF/VFN3-D/VFN380Q.Seq.d/                       1419   0.0
VFN333 (VFN333Q) /CSM/VF/VFN3-B/VFN333Q.Seq.d/                       1419   0.0
VFN222 (VFN222Q) /CSM/VF/VFN2-A/VFN222Q.Seq.d/                       1419   0.0
VFM881 (VFM881Q) /CSM/VF/VFM8-D/VFM881Q.Seq.d/                       1419   0.0
VFM519 (VFM519Q) /CSM/VF/VFM5-A/VFM519Q.Seq.d/                       1419   0.0
VFM401 (VFM401Q) /CSM/VF/VFM4-A/VFM401Q.Seq.d/                       1419   0.0
VFM234 (VFM234Q) /CSM/VF/VFM2-B/VFM234Q.Seq.d/                       1419   0.0
VFL761 (VFL761Q) /CSM/VF/VFL7-C/VFL761Q.Seq.d/                       1419   0.0
VFL410 (VFL410Q) /CSM/VF/VFL4-A/VFL410Q.Seq.d/                       1419   0.0
VFL385 (VFL385Q) /CSM/VF/VFL3-D/VFL385Q.Seq.d/                       1419   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

Y16962|Y16962.1 Dictyostelium discoideum mRNA for cathepsin D.   1419   0.0    1
AJ243946|AJ243946.1 Dictyostelium discoideum ctsD gene for cathepsin D, exons 1 to 2.  1419   0.0    2
E33916|E33916.1 Candida boidinii strain with lowered protease activity and  utilization thereof as host for producing foreign protein.    36   3e-06  5
CD825453|CD825453.1 BN25.060N03F011129 BN25 Brassica napus cDNA clone BN25060N03, mRNA sequence.    62   2e-05  1
CB264579|CB264579.1 51-E015023-035-004-F13-T7R MPIZ-ADIS-035 Arabidopsis thaliana cDNA clone MPIZp2000F134Q 5-PRIME, mRNA sequence.    62   2e-05  1
AL765461|AL765461.1 Arabidopsis thaliana T-DNA flanking sequence GK-139E08-012875.    62   2e-05  1
AL161500|AL161500.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 12.    62   2e-05  1
AV567523|AV567523.1 Arabidopsis thaliana cDNA clone:SQL15g03F, 3' end.    62   2e-05  1
AL762930|AL762930.1 Arabidopsis thaliana T-DNA flanking sequence GK-030C11-011522.    62   2e-05  1
AF372974|AF372974.1 Arabidopsis thaliana AT4g04460/T26N6_7 mRNA, complete cds.    62   2e-05  1
dna update 2004. 3.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EF213114_1(EF213114|pid:none) Penaeus monodon cathepsin D mRNA, ...   244   e-103
(Q03168) RecName: Full=Lysosomal aspartic protease;          EC=...   246   e-102
DQ010007_1(DQ010007|pid:none) Bombyx mori CathD mRNA, complete c...   238   e-100
EF070454_1(EF070454|pid:none) Maconellicoccus hirsutus clone WHM...   242   e-100
AB106552_1(AB106552|pid:none) Todarodes pacificus tpaD mRNA for ...   232   2e-98
FJ168036_1(FJ168036|pid:none) Fasciola hepatica cathepsin D-like...   240   4e-97
EF000001_1(EF000001|pid:none) Fasciola hepatica cathepsin D-like...   234   4e-95
U90750_1(U90750|pid:none) Schistosoma japonicum aspartic proteas...   231   7e-95
L41346_1(L41346|pid:none) Schistosoma japonicum aspartic protein...   231   7e-95
U60995_1(U60995|pid:none) Schistosoma mansoni aspartic proteinas...   229   2e-94
protein update 2009. 4.20
PSORT 

 psg: 0.86  gvh: 0.77  alm: 0.41  top: 0.60  tms: 0.00  mit: 0.32  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: extracellular, including cell wall
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal

>> prediction for AFF394 is exc
5' end seq. ID AFF394F
5' end seq. 
>AFF394F.Seq
AACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTTTTTATTAATCAATTCCACATGA
TTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTT
AGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAG
AGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAAT
TTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGC
CTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCC
AATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATA
TGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGT
CTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGC
----------
Length of 5' end seq. 540
3' end seq. ID AFF394Z
3' end seq. 
>AFF394Z.Seq
----------TGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTT
TCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATT
CAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCT
CCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCG
ATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGG
AATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTT
GTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTG
CCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTA
GCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAA
CTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGAT
TTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTG
CTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAG
GTTAAATTTTTTTAATTAATTTAT
Length of 3' end seq. 734
Connected seq. ID AFF394P
Connected seq. 
>AFF394P.Seq
AACAAAGATTTTGGCGCACAGATTTCAACAAAGATTTTTTTATTAATCAATTCCACATGA
TTCAATAAAGGGGAAAATGAAATTACTTATTTTAACTTTATTTTTAGCTACTATTGTTTT
AGCTCAAGCTTTAACAGTACCATTAAACTTCCATCAAGCTTCAAGAGAATCTAGAAGAAG
AGTTCCACAAAAATGGTCAAACAGATTATCTGCTCTCAATGCTGGTACCACAATCCCAAT
TTCAGATTTTGAAGATGCTCAATACTATGGTGCCATTACCATTGGTACCCCAGGTCAAGC
CTTCAAAGTAGTTTTCGATACTGGTTCATCCAACTTGTGGATTCCATCAAAGAAATGTCC
AATCACTGTTGTTGCATGTGATTTACATAACAAATATAACAGCGGTGCCTCAAGCACATA
TGTCGCCAACGGAACTGATTTCACCATCCAATACGGTAGTGGTGCTATGTCAGGTTTTGT
CTCTCAAGATTCCGTCACTGTTGGTTCATTAACTGTTAAAGATCAATTATTCGCTGAAGC
----------TGTTAAAGATCAATTATTCGCTGAAGCCACTGCCGAACCAGGTATTGCTT
TCGATTTCGCCAAATTCGATGGTATTTTAGGTCTTGCTTTCCAATCCATCTCTGTTAATT
CAATTCCACCAGTCTTTTACAACATGTTATCACAAGGTTTAGTTTCATCAACACTCTTCT
CCTTCTGGTTATCAAGAACTCCAGGTGCCAACGGTGGTGAACTCTCATTCGGTTCAATCG
ATAACACCAAATACACTGGTGACATTACCTACGTCCCATTAACCAACGAAACCTATTGGG
AATTCGTTATGGATGACTTTGCTATCGATGGTCAATCAGCTGGTTTCTGTGGTACTACTT
GTCACGCAATTTGCGATTCAGGTACATCACTCATTGCTGGTCCAATGGCTGATATTACTG
CCCTCAATGAAAAATTAGGTGCTGTCATCTTAAATGGTGAAGGTGTCTTCTCTGATTGTA
GCGTTATCAACACCTTACCAAATGTTACCATCACCGTTGCTGGTCGTGAATTTGTTTTAA
CTCCAAAAGAATACGTTTTAGAAGTTACTGAGTTCGGAAAGACTGAATGTTTGAGTGGAT
TTATGGGTATCGAGTTAAACATGGGAAATTTCTGGATCCTTGGTGATGTTTTCATCTCTG
CTTACTATACTGTATTCGATTTTGGTAATAAACAAGTTGGTTTCGCAACTGCCATTCAAG
GTTAAATTTTTTTAATTAATTTAT
Length of connected seq. 1274
Full length Seq ID -
Full length Seq. -
Length of full length seq. -