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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB865
Library CH
(Link to library)
Clone ID CHB865
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15579-1
Original site URL
Representative seq. ID CHB865P
(Link to Original site)
Representative DNA sequence 
>CHB865 (CHB865Q) /CSM/CH/CHB8-C/CHB865Q.Seq.d/
AATATTATTTATATATTTATAGTAACATTATATGAATATTTAAAAATTGGAAAACATTTT
TTAAAAATTGGAAAACATTATTAAAAAAAGAAATAAATTGATTTTTAATTATAAAAAAAA
AAXXXXXXXXXXGAATCTTGTGAAATAGGTTTTGGTTGTCTTGCAATTCCAAAAAATTGT
AATGATAATGATCCATGTACCACTGATCATTGTGATCNAGCTATAGGTTGTTATTATGAT
AAGTTTGATAATTGTGATGCATGTAATGCTGTAGATACTTGTATTACAAATGATTTATGC
TTCCCAAGGGAATGTAATCCAAGAGGGAACCCACCTTGTTTAATAAACCCTATTAATTGT
ACAAGCACTGATCCATGCATTTTTTCATATTGTGAAAATGGTGTATGTATTCCAACATAC
ATTTGTACTCCAACTCCATCTGTAACTCCAACTGTTACCCCAACTGTTACTCCAACTGTT
ACCCCAACTGTTACACCAACCGTTACTCCAACCGTTACCCCAACTCCAACTACAACTCCA
ACTCCATCACCAACCACTGTCCCACCAAGACCAACCCCAACTCC
sequence update 2002.10.25
Translated Amino Acid sequence 
ilfiyl**hymni*klenif*KLENIIKKRNKLIFNYKKK---

---ESCEIGFGCLAIPKNCNDNDPCTTDHCDXAIGCYYDKFDNCDACNAVDTCITNDLCF
PRECNPRGNPPCLINPINCTSTDPCIFSYCENGVCIPTYICTPTPSVTPTVTPTVTPTVT
PTVTPTVTPTVTPTPTTTPTPSPTTVPPRPTPT
Translated Amino Acid sequence (All Frames) 
Frame A:
niiyifivtlyeylkigkhflkigkhy*kkk*idf*l*kk---

---ESCEIGFGCLAIPKNCNDNDPCTTDHCDXAIGCYYDKFDNCDACNAVDTCITNDLCF
PRECNPRGNPPCLINPINCTSTDPCIFSYCENGVCIPTYICTPTPSVTPTVTPTVTPTVT
PTVTPTVTPTVTPTPTTTPTPSPTTVPPRPTPT

Frame B:
ilfiyl**hymni*klenif*KLENIIKKRNKLIFNYKKK---

---nlvk*vlvvlqfqkivmimihvpliivixl*vvimisliivmhvml*ilvlqmiyas
qgnviqegthlv**tllivqalihaffhivkmvyvfqhtfvlqlhl*lqllpqlllqllp
qllhqpllqplpqlqlqlqlhhqplshqdqpql

Frame C:
yylyiysnii*ifknwktffknwktllkkein*fliikkk---

---il*nrfwlscnskkl****smyh*sl*ssyrlll**v**l*cm*ccrylyyk*fmlp
kgm*skreptlfnkpy*lykh*smhffil*kwcmysnihlysnsicnsncypncysncyp
ncytnrysnrypnsnynsnsitnhcptktnpns
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHD753 (CHD753Q) /CSM/CH/CHD7-C/CHD753Q.Seq.d/                        890   0.0
CHB865 (CHB865Q) /CSM/CH/CHB8-C/CHB865Q.Seq.d/                        890   0.0
AFJ801 (AFJ801Q) /CSM/AF/AFJ8-A/AFJ801Q.Seq.d/                        890   0.0
SFI265 (SFI265Q) /CSM/SF/SFI2-C/SFI265Q.Seq.d/                        888   0.0
CHB694 (CHB694Q) /CSM/CH/CHB6-D/CHB694Q.Seq.d/                        882   0.0
CFC681 (CFC681Q) /CSM/CF/CFC6-D/CFC681Q.Seq.d/                        870   0.0
CFF487 (CFF487Q) /CSM/CF/CFF4-D/CFF487Q.Seq.d/                        842   0.0
CHH341 (CHH341Q) /CSM/CH/CHH3-B/CHH341Q.Seq.d/                        827   0.0
CHC630 (CHC630Q) /CSM/CH/CHC6-B/CHC630Q.Seq.d/                        825   0.0
CFB225 (CFB225Q) /CSM/CF/CFB2-B/CFB225Q.Seq.d/                        817   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CK261949|CK261949.1 EST708027 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABD21 5' end, mRNA sequence.    40   3e-06  3
AC124744|AC124744.2 Mus musculus chromosome 13 clone RP23-223E16, WORKING DRAFT SEQUENCE, 3 unordered pieces.    54   6e-06  5
AC125190|AC125190.2 Mus musculus chromosome UNK clone RP23-288O24, WORKING DRAFT SEQUENCE, 3 unordered pieces.    54   2e-05  2
X16057|X16057.1 Dictyostelium discoideum DNA for cyclic nucleotide phosphodiesterase inhibitor.    56   7e-04  1
M20909|M20909.1 Dictyostelium discoideum cell surface glycoprotein PsA precursor (D19) gene, complete cds.    46   0.001  2
BP128775|BP128775.1 Nicotiana tabacum cDNA, clone: BY567, primer: M13 forward, sequence 1.    54   0.003  2
X15980|X15980.1 Dictyostelium discoideum D19 gene 5'-flanking region.    46   0.003  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   0.004  10
BE342251|BE342251.1 EST395095 potato stolon, Cornell University Solanum tuberosum cDNA clone cSTA19G12, mRNA sequence.    38   0.006  2
AC149039|AC149039.1 Medicago truncatula chromosome 7 clone mth2-4g23, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    42   0.006  3
dna update 2004. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54FD8) RecName: Full=Protein psiD; Flags: Precursor;                231   8e-60
(Q1ZXC5) RecName: Full=Protein psiC; Flags: Precursor;                 93   4e-18
(Q54C31) RecName: Full=Protein psiR; Flags: Precursor;                 72   8e-12
(Q54C32) RecName: Full=Protein psiQ; Flags: Precursor;                 69   7e-11
AC117072_61(AC117072|pid:none) Dictyostelium discoideum chromoso...    66   8e-10
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                 64   4e-09
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    61   2e-08
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                 60   4e-08
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    57   5e-07
A26838(A26838)prestalk protein precursor - slime mold (Dictyoste...    57   5e-07
protein update 2009. 3.29
PSORT 

 psg: 0.52  gvh: 0.27  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CHB865 is nuc
5' end seq. ID CHB865F
5' end seq. 
>CHB865F.Seq
AATATTATTTATATATTTATAGTAACATTATATGAATATTTAAAAATTGGAAAACATTTT
TTAAAAATTGGAAAACATTATTAAAAAAAGAAATAAATTGATTTTTAATTATAAAAAAAA
AANNNNNNNNNN
Length of 5' end seq. 132
3' end seq. ID CHB865Z
3' end seq. 
>CHB865Z.Seq
NNNNNNNNNNGAATCTTGTGAAATAGGTTTTGGTTGTCTTGCAATTCCAAAAAATTGTAA
TGATAATGATCCATGTACCACTGATCATTGTGATCNAGCTATAGGTTGTTATTATGATAA
GTTTGATAATTGTGATGCATGTAATGCTGTAGATACTTGTATTACAAATGATTTATGCTT
CCCAAGGGAATGTAATCCAAGAGGGAACCCACCTTGTTTAATAAACCCTATTAATTGTAC
AAGCACTGATCCATGCATTTTTTCATATTGTGAAAATGGTGTATGTATTCCAACATACAT
TTGTACTCCAACTCCATCTGTAACTCCAACTGTTACCCCAACTGTTACTCCAACTGTTAC
CCCAACTGTTACACCAACCGTTACTCCAACCGTTACCCCAACTCCAACTACAACTCCAAC
TCCATCACCAACCACTGTCCCACCAAGACCAACCCCAACTCC
Length of 3' end seq. 462
Connected seq. ID CHB865P
Connected seq. 
>CHB865P.Seq
AATATTATTTATATATTTATAGTAACATTATATGAATATTTAAAAATTGGAAAACATTTT
TTAAAAATTGGAAAACATTATTAAAAAAAGAAATAAATTGATTTTTAATTATAAAAAAAA
AA----------GAATCTTGTGAAATAGGTTTTGGTTGTCTTGCAATTCCAAAAAATTGT
AATGATAATGATCCATGTACCACTGATCATTGTGATCNAGCTATAGGTTGTTATTATGAT
AAGTTTGATAATTGTGATGCATGTAATGCTGTAGATACTTGTATTACAAATGATTTATGC
TTCCCAAGGGAATGTAATCCAAGAGGGAACCCACCTTGTTTAATAAACCCTATTAATTGT
ACAAGCACTGATCCATGCATTTTTTCATATTGTGAAAATGGTGTATGTATTCCAACATAC
ATTTGTACTCCAACTCCATCTGTAACTCCAACTGTTACCCCAACTGTTACTCCAACTGTT
ACCCCAACTGTTACACCAACCGTTACTCCAACCGTTACCCCAACTCCAACTACAACTCCA
ACTCCATCACCAACCACTGTCCCACCAAGACCAACCCCAACTCC
Length of connected seq. 574
Full length Seq ID -
Full length Seq. -
Length of full length seq. -