>CHB648 (CHB648Q) /CSM/CH/CHB6-B/CHB648Q.Seq.d/
ATTGTCATAATAATATTATAAAATTCAAAGATGTCTAAAGTAGTCCGTAGTAGTAAATAT
CGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTATCAAAACTTAAAAGTTACC
AAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGATACTTTGGTGTTATTTGG
GATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAGGCTTCAGGTAAAACCACA
TCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGATATTGCTTTCCACCCATTC
AATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATTTGTATTTGGGGTATCCCA
GAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACTTTATCTGGTCACAAGAGA
AAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTTGCCGTTACCTCATCTGGT
GATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAATTTAXXXXXXXXXX

iviiil*NSKMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTRYFGVIW
DAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNICIWGIP
EGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKN---

Frame A:
iviiil*NSKMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTRYFGVIW
DAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNICIWGIP
EGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKN---


Frame B:
ls**yykiqrclk*svvvnivmflqhnqkkksaikt*klpnqhgivitlqptldtlvlfg
mlqvvvhsllfhmrlqvkphqfhysmvirvqf*illsthsmktslvqflkivtfvfgvsq
kvv*ptqsqphsklylvtrerlvpshsiqllimlplphlvis*lklgmlnkvri---


Frame C:
chnniikfkdv*ssp***isscfcsttkkrrvlsklksyqismg**lrcsqh*ilwcylg
ccrwwfircyst*gfr*nhissiiqws*ecsfrycfppiq*kprwfsf*rl*hlylgypr
rwfnrlnlnptpnfiwsqekgwyhliqssc**ccryliw*fls*nlgc*tr*ef---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK803 (VHK803Q) /CSM/VH/VHK8-A/VHK803Q.Seq.d/                        999   0.0
VFL210 (VFL210Q) /CSM/VF/VFL2-A/VFL210Q.Seq.d/                        999   0.0
VFK727 (VFK727Q) /CSM/VF/VFK7-B/VFK727Q.Seq.d/                        999   0.0
VFI347 (VFI347Q) /CSM/VF/VFI3-B/VFI347Q.Seq.d/                        999   0.0
VFH410 (VFH410Q) /CSM/VF/VFH4-A/VFH410Q.Seq.d/                        999   0.0
VFH268 (VFH268Q) /CSM/VF/VFH2-C/VFH268Q.Seq.d/                        999   0.0
VFF460 (VFF460Q) /CSM/VF/VFF4-C/VFF460Q.Seq.d/                        999   0.0
VFD493 (VFD493Q) /CSM/VF/VFD4-D/VFD493Q.Seq.d/                        999   0.0
VFC804 (VFC804Q) /CSM/VF/VFC8-A/VFC804Q.Seq.d/                        999   0.0
SFJ793 (SFJ793Q) /CSM/SF/SFJ7-D/SFJ793Q.Seq.d/                        999   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X61480|X61480.1 D.discoideum mRNA for coronin.                    846   0.0    2
AZ542600|AZ542600.1 ENTET32TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36   0.079  2
BH132994|BH132994.1 ENTNE76TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36   0.081  2
AC142359|AC142359.3 Rattus norvegicus clone CH230-81G8, WORKING DRAFT SEQUENCE, 44 unordered pieces.    34   0.24   5
BP561937|BP561937.1 Arabidopsis thaliana cDNA clone:RAFL09-34-C08, 5' end.    34   0.63   2
BZ396696|BZ396696.1 EINDS48TF EI_10_12_KB Entamoeba invadens genomic clone EINDS48, DNA sequence.    40   1.0    2
AC111251|AC111251.5 Rattus norvegicus clone CH230-13G13, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    36   1.4    4
AC110479|AC110479.4 Rattus norvegicus clone CH230-38O22, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    36   2.0    4
AZ675137|AZ675137.1 ENTJY92TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44   2.3    1
AC140743|AC140743.1 Rattus norvegicus clone CH230-468J6, WORKING DRAFT SEQUENCE, 29 unordered pieces.    34   2.5    6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S18663(S18663) coronin - slime mold (Dictyostelium discoideum)  ...   338   4e-92
AY525781_1(AY525781|pid:none) Acanthamoeba healyi coronin mRNA, ...   186   3e-46
AP006852_172(AP006852|pid:none) Candida albicans genomic DNA, ch...   174   1e-42
FM992694_169(FM992694|pid:none) Candida dubliniensis CD36 chromo...   173   2e-42
BT042868_1(BT042868|pid:none) Zea mays full-length cDNA clone ZM...   169   5e-41
(Q06440) RecName: Full=Coronin-like protein;  &AY693023_1(AY6930...   163   3e-39
AM920427_1306(AM920427|pid:none) Penicillium chrysogenum Wiscons...   162   4e-39
CR382127_605(CR382127|pid:none) Yarrowia lipolytica strain CLIB1...   161   8e-39
AE013599_228(AE013599|pid:none) Drosophila melanogaster chromoso...   157   2e-37
AY190677_1(AY190677|pid:none) Pagrus major actin-binding protein...   157   2e-37

 psg: 0.58  gvh: 0.32  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.04
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for CHB648 is mit

>CHB648F.Seq
ATTGTCATAATAATATTATAAAATTCAAAGATGTCTAAAGTAGTCCGTAGTAGTAAATAT
CGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTATCAAAACTTAAAAGTTACC
AAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGATACTTTGGTGTTATTTGG
GATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAGGCTTCAGGTAAAACCACA
TCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGATATTGCTTTCCACCCATTC
AATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATTTGTATTTGGGGTATCCCA
GAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACTTTATCTGGTCACAAGAGA
AAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTTGCCGTTACCTCATCTGGT
GATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAATTTANNNNNNNNNN