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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF372
Library AF
(Link to library)
Clone ID AFF372
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U10856-1
Original site URL
Representative seq. ID AFF372P
(Link to Original site)
Representative DNA sequence 
>AFF372 (AFF372Q) /CSM/AF/AFF3-C/AFF372Q.Seq.d/
ATACGAATAACACAAACACAAATATAAATATATACACATACATACATACACACACACATA
AATAGAGAAAAAAAAAATTATAAAAGTTTAAGATGATTATTGCAGTTAGATCACCAAGTG
GTACAACAAAAATCAATGTTAGCGATACAGCTATTGTTAAAGATTTATATGTAAAATTAA
AAGAAAATAATGTAGATATAGATGGTAAATCAATTACCAAAACACCAAGAGACCCACCAT
TAGAAGTTGGTGATAAATTATCATCACTTGGTATAAAGAGTGGAGATTTATTACATTTAG
TTGGAAATAATAATAATAATAATAATGATAATAAAGCTAGTAGCGGTAGTAATAATAATA
ATAATAATAATAATATTAGTAAAAAAGAAATATCATATGATGAAAAATTACCAAATGGAT
TAACACCTAGATGTAAACATGCAGTTGGTGATAAATGTGTATTTTGTTCAGATGCACAAG
GTATGAAAAGATGTGATCATCCAGAGAATGCAACATGTCCAAATTGTCAAAATAAGAAAT
TTAAAGGTAAAAGATTAAAATGGTTATGTAATCATCCAGCAGGTGGTAAATGTTCAAATT
GTTTAAGGGTTGGAAAGAATGTATCATACAAATGTAATCATGGTTCAACCGCTACTTGTG
TAAATTGTATGGATAAAGATGATGAAGATCAAGATGATAATGAGAAGATAATAAAGATAA
TAAAGATAATTCAGAAAATTAAAAAAAGTGGTTTGTGCCAAAAGXXXXXXXXXXTTGATA
GTATCTATGAGCCACCACAAGAATGTAAGGATAAACAAACTCCAACACTCTTACCAGATC
CATTGGCTGATAAGATTGAATCAATGGCATCAATGTTGGGTTTGACAAGAGTTGGTTGGA
TCTTTTCACATCCATCAAGAAAGTATACAATGTCATCCACTGAAATCATTCAAGCAGCTT
CCTATCAAAATAAGTATGGTCCAAGCTTTGTCACTTTAATCCTCTCGGTCAATAGTGATG
GTCAAAGTAATATGGAGGCTTTCCAAGTATCTGATCAAGCACTTAAATTGGAGAAAACCG
GAGAATTCCTCCCAACTCAACCTGATCCAACCAAATGTAAATTAAAATCACCAGTTTTTG
AAGAAGGCACTGAAACTATCAATGCCGATACTTACTTTTTCATCGTTACCGTTCCATTGA
AGGCAAGAGAAGATAAATCCATCTTTAACATCTCTTTCCCAGTGGAAAATAGAATACCTG
TAAATACCCTCTCGGATTTGGCCTCTTATAAACTCGAGCATAAAGATGTCTCTCCTTTGA
AATTCTTTTCAGATTTCCATTTCCTTATCTTTTTATTAGAGAATCAATTCCTTGACTTTC
AATCGGATTTCCCAATCATTTGTGAAAATATCCGTTCAAGATCAAGTGAAAATTTAATTG
GTTATTTAGAAATTATAAATTATCAAATGGNATATCAT
sequence update 2003. 5. 2
Translated Amino Acid sequence 
tnntntniniytyihththk*rkkkl*KFKMIIAVRSPSGTTKINVSDTAIVKDLYVKLK
ENNVDIDGKSITKTPRDPPLEVGDKLSSLGIKSGDLLHLVGNNNNNNNDNKASSGSNNNN
NNNNISKKEISYDEKLPNGLTPRCKHAVGDKCVFCSDAQGMKRCDHPENATCPNCQNKKF
KGKRLKWLCNHPAGGKCSNCLRVGKNVSYKCNHGSTATCVNCMDKDDEDQDDNEKIIKII
KIIQKIKKSGLCQK---

---DSIYEPPQECKDKQTPTLLPDPLADKIESMASMLGLTRVGWIFSHPSRKYTMSSTEI
IQAASYQNKYGPSFVTLILSVNSDGQSNMEAFQVSDQALKLEKTGEFLPTQPDPTKCKLK
SPVFEEGTETINADTYFFIVTVPLKAREDKSIFNISFPVENRIPVNTLSDLASYKLEHKD
VSPLKFFSDFHFLIFLLENQFLDFQSDFPIICENIRSRSSENLIGYLEIINYQMXYH
Translated Amino Acid sequence (All Frames) 
Frame A:
iritqtqi*iythtyihthinrekknykslr*llqldhqvvqqksmlaiqlllkiym*n*
kkim*i*mvnqlpkhqethh*klvinyhhlv*rveiyyi*leiiiiiimiiklvavviii
iiiiilvkkkyhmmknyqmd*hldvnmqlvinvyfvqmhkv*kdviiqrmqhvqivkirn
lkvkd*ngyviiqqvvnvqiv*glermyhtnvimvqpllv*ivwikmmkikmimrr**r*
*r*frklkkvvcak---

---livsmshhknvrinklqhsyqihwlirlnqwhqcwv*qelvgsfhihqesiqchplk
sfkqlpikismvqalsl*ssrsivmvkviwrlskylikhlnwrkpenssqlnliqpnvn*
nhqflkkalklsmpiltfsslpfh*rqekinpsltslsqwkieyl*ipsriwplinssik
msll*nsfqisislsfy*rinsltfnrisqsfvkisvqdqvki*lvi*kl*iikwxi

Frame B:
ye*hkhkykyihihtytht*iekkkiikv*ddycs*itkwynknqc*rysyc*rfickik
rk*cryrw*inyqntkrptirsw**iiitwykewrfitfswk*********s**r*****
****y**krnii**kitkwint*m*tcsw**mcilfrctryekm*ssrecnmsklsk*ei
*r*kikmvm*sssrw*mfklfkgwkeciiqm*swfnrylcklyg*r**rsr***ednkdn
kdnsen*kkwfvpk---

---**yl*attrm*g*tnsntltrsig**d*inginvgfdkswldlftsikkvynvih*n
hsssflsk*vwsklchfnplgq**wsk*yggfpsi*sst*igenrrippnst*snqm*ik
itsf*rrh*nyqcryllfhryrsiegkrr*ihl*hlfpsgk*ntckyplgfgll*tra*r
clsfeilfrfpfpylfiresip*lsigfpnhl*kypfkik*kfnwlfrnyklsngis

Frame C:
tnntntniniytyihththk*rkkkl*KFKMIIAVRSPSGTTKINVSDTAIVKDLYVKLK
ENNVDIDGKSITKTPRDPPLEVGDKLSSLGIKSGDLLHLVGNNNNNNNDNKASSGSNNNN
NNNNISKKEISYDEKLPNGLTPRCKHAVGDKCVFCSDAQGMKRCDHPENATCPNCQNKKF
KGKRLKWLCNHPAGGKCSNCLRVGKNVSYKCNHGSTATCVNCMDKDDEDQDDNEKIIKII
KIIQKIKKSGLCQK---

---DSIYEPPQECKDKQTPTLLPDPLADKIESMASMLGLTRVGWIFSHPSRKYTMSSTEI
IQAASYQNKYGPSFVTLILSVNSDGQSNMEAFQVSDQALKLEKTGEFLPTQPDPTKCKLK
SPVFEEGTETINADTYFFIVTVPLKAREDKSIFNISFPVENRIPVNTLSDLASYKLEHKD
VSPLKFFSDFHFLIFLLENQFLDFQSDFPIICENIRSRSSENLIGYLEIINYQMXYH
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF372 (AFF372Q) /CSM/AF/AFF3-C/AFF372Q.Seq.d/                       2079   0.0
CFI394 (CFI394Q) /CSM/CF/CFI3-D/CFI394Q.Seq.d/                       1245   0.0
SHB513 (SHB513Q) /CSM/SH/SHB5-A/SHB513Q.Seq.d/                        450   e-125
VHO815 (VHO815Q) /CSM/VH/VHO8-A/VHO815Q.Seq.d/                         52   2e-05
VHO808 (VHO808Q) /CSM/VH/VHO8-A/VHO808Q.Seq.d/                         52   2e-05
VHJ739 (VHJ739Q) /CSM/VH/VHJ7-B/VHJ739Q.Seq.d/                         52   2e-05
VHJ184 (VHJ184Q) /CSM/VH/VHJ1-D/VHJ184Q.Seq.d/                         52   2e-05
VHC329 (VHC329Q) /CSM/VH/VHC3-B/VHC329Q.Seq.d/                         52   2e-05
VHA666 (VHA666Q) /CSM/VH/VHA6-C/VHA666Q.Seq.d/                         52   2e-05
VHA435 (VHA435Q) /CSM/VH/VHA4-B/VHA435Q.Seq.d/                         52   2e-05
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   4e-04  27
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    34   4e-04  24
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    50   6e-04  19
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           34   0.001  25
AL929355|AL929355.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 1/5.    44   0.001  17
AF063866|AF063866.1 Melanoplus sanguinipes entomopoxvirus, complete genome.    36   0.002  16
AL929354|AL929354.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 4/4.    36   0.005  18
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   0.006  18
AZ679333|AZ679333.1 ENTIN44TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    54   0.006  1
AF193536|AF193536.1 Emberiza citrinella clone QmAAT37 microsatellite sequence.    54   0.006  1
dna update 2004. 3.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT040138_1(BT040138|pid:none) Zea mays full-length cDNA clone ZM...   105   4e-21
EU965794_1(EU965794|pid:none) Zea mays clone 289005 NPL4 family ...   104   9e-21
EF145895_1(EF145895|pid:none) Populus trichocarpa clone WS0114_B...   101   8e-20
(O82264) RecName: Full=NPL4-like protein 2;  &AC005309_33(AC0053...    98   6e-19
AM910992_254(AM910992|pid:none) Plasmodium knowlesi strain H chr...    96   3e-18
(Q9ES54) RecName: Full=Nuclear protein localization protein 4 ho...    95   7e-18
AK023046_1(AK023046|pid:none) Homo sapiens cDNA FLJ12984 fis, cl...    94   1e-17
AL834300_1(AL834300|pid:none) Homo sapiens mRNA; cDNA DKFZp762C2...    94   1e-17
AK294473_1(AK294473|pid:none) Homo sapiens cDNA FLJ57969 complet...    94   1e-17
AB040932_1(AB040932|pid:none) Homo sapiens mRNA for KIAA1499 pro...    94   1e-17
protein update 2009. 3.28
PSORT 

 psg: 0.74  gvh: 0.36  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFF372 is nuc
5' end seq. ID AFF372F
5' end seq. 
>AFF372F.Seq
ATACGAATAACACAAACACAAATATAAATATATACACATACATACATACACACACACATA
AATAGAGAAAAAAAAAATTATAAAAGTTTAAGATGATTATTGCAGTTAGATCACCAAGTG
GTACAACAAAAATCAATGTTAGCGATACAGCTATTGTTAAAGATTTATATGTAAAATTAA
AAGAAAATAATGTAGATATAGATGGTAAATCAATTACCAAAACACCAAGAGACCCACCAT
TAGAAGTTGGTGATAAATTATCATCACTTGGTATAAAGAGTGGAGATTTATTACATTTAG
TTGGAAATAATAATAATAATAATAATGATAATAAAGCTAGTAGCGGTAGTAATAATAATA
ATAATAATAATAATATTAGTAAAAAAGAAATATCATATGATGAAAAATTACCAAATGGAT
TAACACCTAGATGTAAACATGCAGTTGGTGATAAATGTGTATTTTGTTCAGATGCACAAG
GTATGAAAAGATGTGATCATCCAGAGAATGCAACATGTCCAAATTGTCAAAATAAGAAAT
TTAAAGGTAAAAGATTAAAATGGTTATGTAATCATCCAGCAGGTGGTAAATGTTCAAATT
GTTTAAGGGTTGGAAAGAATGTATCATACAAATGTAATCATGGTTCAACCGCTACTTGTG
TAAATTGTATGGATAAAGATGATGAAGATCAAGATGATAATGAGAAGATAATAAAGATAA
TAAAGATAATTCAGAAAATTAAAAAAAGTGGTTTGTGCCAAAAGNNNNNNNNNN
Length of 5' end seq. 774
3' end seq. ID AFF372Z
3' end seq. 
>AFF372Z.Seq
----------TTGATAGTATCTATGAGCCACCACAAGAATGTAAGGATAAACAAACTCCA
ACACTCTTACCAGATCCATTGGCTGATAAGATTGAATCAATGGCATCAATGTTGGGTTTG
ACAAGAGTTGGTTGGATCTTTTCACATCCATCAAGAAAGTATACAATGTCATCCACTGAA
ATCATTCAAGCAGCTTCCTATCAAAATAAGTATGGTCCAAGCTTTGTCACTTTAATCCTC
TCGGTCAATAGTGATGGTCAAAGTAATATGGAGGCTTTCCAAGTATCTGATCAAGCACTT
AAATTGGAGAAAACCGGAGAATTCCTCCCAACTCAACCTGATCCAACCAAATGTAAATTA
AAATCACCAGTTTTTGAAGAAGGCACTGAAACTATCAATGCCGATACTTACTTTTTCATC
GTTACCGTTCCATTGAAGGCAAGAGAAGATAAATCCATCTTTAACATCTCTTTCCCAGTG
GAAAATAGAATACCTGTAAATACCCTCTCGGATTTGGCCTCTTATAAACTCGAGCATAAA
GATGTCTCTCCTTTGAAATTCTTTTCAGATTTCCATTTCCTTATCTTTTTATTAGAGAAT
CAATTCCTTGACTTTCAATCGGATTTCCCAATCATTTGTGAAAATATCCGTTCAAGATCA
AGTGAAAATTTAATTGGTTATTTAGAAATTATAAATTATCAAATGGNATATCAT
Length of 3' end seq. 704
Connected seq. ID AFF372P
Connected seq. 
>AFF372P.Seq
ATACGAATAACACAAACACAAATATAAATATATACACATACATACATACACACACACATA
AATAGAGAAAAAAAAAATTATAAAAGTTTAAGATGATTATTGCAGTTAGATCACCAAGTG
GTACAACAAAAATCAATGTTAGCGATACAGCTATTGTTAAAGATTTATATGTAAAATTAA
AAGAAAATAATGTAGATATAGATGGTAAATCAATTACCAAAACACCAAGAGACCCACCAT
TAGAAGTTGGTGATAAATTATCATCACTTGGTATAAAGAGTGGAGATTTATTACATTTAG
TTGGAAATAATAATAATAATAATAATGATAATAAAGCTAGTAGCGGTAGTAATAATAATA
ATAATAATAATAATATTAGTAAAAAAGAAATATCATATGATGAAAAATTACCAAATGGAT
TAACACCTAGATGTAAACATGCAGTTGGTGATAAATGTGTATTTTGTTCAGATGCACAAG
GTATGAAAAGATGTGATCATCCAGAGAATGCAACATGTCCAAATTGTCAAAATAAGAAAT
TTAAAGGTAAAAGATTAAAATGGTTATGTAATCATCCAGCAGGTGGTAAATGTTCAAATT
GTTTAAGGGTTGGAAAGAATGTATCATACAAATGTAATCATGGTTCAACCGCTACTTGTG
TAAATTGTATGGATAAAGATGATGAAGATCAAGATGATAATGAGAAGATAATAAAGATAA
TAAAGATAATTCAGAAAATTAAAAAAAGTGGTTTGTGCCAAAAG----------TTGATA
GTATCTATGAGCCACCACAAGAATGTAAGGATAAACAAACTCCAACACTCTTACCAGATC
CATTGGCTGATAAGATTGAATCAATGGCATCAATGTTGGGTTTGACAAGAGTTGGTTGGA
TCTTTTCACATCCATCAAGAAAGTATACAATGTCATCCACTGAAATCATTCAAGCAGCTT
CCTATCAAAATAAGTATGGTCCAAGCTTTGTCACTTTAATCCTCTCGGTCAATAGTGATG
GTCAAAGTAATATGGAGGCTTTCCAAGTATCTGATCAAGCACTTAAATTGGAGAAAACCG
GAGAATTCCTCCCAACTCAACCTGATCCAACCAAATGTAAATTAAAATCACCAGTTTTTG
AAGAAGGCACTGAAACTATCAATGCCGATACTTACTTTTTCATCGTTACCGTTCCATTGA
AGGCAAGAGAAGATAAATCCATCTTTAACATCTCTTTCCCAGTGGAAAATAGAATACCTG
TAAATACCCTCTCGGATTTGGCCTCTTATAAACTCGAGCATAAAGATGTCTCTCCTTTGA
AATTCTTTTCAGATTTCCATTTCCTTATCTTTTTATTAGAGAATCAATTCCTTGACTTTC
AATCGGATTTCCCAATCATTTGTGAAAATATCCGTTCAAGATCAAGTGAAAATTTAATTG
GTTATTTAGAAATTATAAATTATCAAATGGNATATCAT
Length of connected seq. 1468
Full length Seq ID -
Full length Seq. -
Length of full length seq. -