>AFA547 (AFA547Q) /CSM/AF/AFA5-B/AFA547Q.Seq.d/
XXXXXXXXXXCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAA
TCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTG
ATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCC
CAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAG
AATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATG
AACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTG
GTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTCTG
AAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCG
CCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAA
AAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAA
CTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT

---KDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAP
TIANPEYKGEWKAKKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSG
SIFNNMIVXDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEK
EAEEADEEEEEVAEEDLVKTDXKKEEVKKSTKK

Frame A:
---KDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAP
TIANPEYKGEWKAKKIPNPEYKGEWXHPLIDNPEYAEDNELYLFNDLGAIGFELWQVKSG
SIFNNMIVXDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAEK
EAEEADEEEEEVAEEDLVKTDXKKEEVKKSTKK


Frame B:
---kiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpq
pspiqntkvngkqrksqiqntkvngxth*litqntlktmnstslmisvlsvsnygklnlv
pslti*ssptlskklkislkrpllptkkpkrkclttskppklkrerkltkn*lpkkllkk
kpkklmkkkkkslkki*lklmxkkkksknqpkk


Frame C:
---rsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspn
hrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iwf
hl*qydrxrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*kr
srrs**rrrrsr*rrfs*n*xqkrrsqkinqks

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        922   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        922   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        922   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        922   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        922   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        922   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        922   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        922   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        922   0.0
VFA823 (VFA823Q) /CSM/VF/VFA8-A/VFA823Q.Seq.d/                        922   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   922  0.0  1
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74  2e-14  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    74  2e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  2e-09  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  2e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  2e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  2e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  2e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  2e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  2e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   285   1e-75
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   177   2e-43
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   176   5e-43
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   175   1e-42
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   174   2e-42
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   173   4e-42
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   172   7e-42
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   172   9e-42
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   171   1e-41
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   171   1e-41

 psg: 0.49  gvh: 0.24  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for AFA547 is nuc

>AFA547Z.Seq
----------CAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAA
TCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTG
ATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCC
CAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAG
AATACAAAGGTGAATGGGNACACCCATTAATTGATAACCCAGAATACGCTGAAGACAATG
AACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTG
GTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTCTCTG
AAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCG
CCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAA
AAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAA
CTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGT