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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF355
Library AF
(Link to library)
Clone ID AFF355
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15580-1
Original site URL
Representative seq. ID AFF355P
(Link to Original site)
Representative DNA sequence 
>AFF355 (AFF355Q) /CSM/AF/AFF3-C/AFF355Q.Seq.d/
ATTTTTGTGTTGTGTGTTTAAAATTTTTTTTCCAGATCTTAATATTTATGAAAATATAAA
AATAACAAAACAGTAATAAGAATATAAATAAATAAAAAAATAAAAAAAAAAATAAAAAAT
GGCAGGTGATGATGATAGAAATTCAATTCCAATGGAACATTATGACCAACAAGAATTACN
ACCACCACAACAAGAATTACAACAACCACCACAACAACAACAACAACAACAACAAGAATT
ACAACAACAACAACAACAACAACAACAAATNTATCCAAGTATAAATGATTTAGATTCTTC
AAGAAATAATTCACCACAAGTACCACATTATGCAAGTAAGAATTCATTTAATAATAATAA
TAGTAGTAGTAATCAATCATTAGAAAAAATACAAGAATTGGAAAGTAAAAAAGAACTTGC
CTATGAACATTTAAGTCCAGCACCACAATCATNATCGTATGAAGATTTAGGATTAAAAGA
TCAAGTTTTAGAGXXXXXXXXXXGAAGTTATAAATGTAATTTATGATCAAATTTCAATTT
ATTTAACAATGTTTGAAAAACATCCAACTTGGACAAAATTTAAAATTCAAAATNACTTTA
AAATTATTTGTTTTTAAAATTGTAAATGTTATTATTTTATATATTCTTAGAGATAGAATA
TTTGAAAATATTACTCATCAATCAATTTTAGAAGGTTGTCCATTTGTTGACGTTGGTTCT
CAATTTTTATTTATTTTAGTTTTAGATTTAACTTTATCAAATCTTTGGGAAATTATTCGA
TGGTGATATTCCAGTTTCTGATTCTTCATCTTCAAGTACGGAAAGGAATTGGTATTAAAA
TCTAAAAAAAATAAAAAATATAAAAAAAAATATAAAAAAATAAAGAAA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
flccvfkiffpdlniyenikitkq**eyk*ikk*kkk*KMAGDDDRNSIPMEHYDQQELX
PPQQELQQPPQQQQQQQQELQQQQQQQQQXYPSINDLDSSRNNSPQVPHYASKNSFNNNN
SSSNQSLEKIQELESKKELAYEHLSPAPQSXSYEDLGLKDQVLE---

---EVINVIYDQISIYLTMFEKHPTWTKFKIQNXFKIICF*nckcyyfiys*r*ni*kyy
ssinfrrlsic*rwfsifiyfsfrfnfikslgnysmvifqflilhlqvrkgigiki*kk*
ki*kki*knke
Translated Amino Acid sequence (All Frames) 
Frame A:
ifvlcv*nffsrs*yl*kyknnktviri*inkkikkkikngr****kfnsngtl*ptrit
ttttrittttttttttttritttttttttnxskyk*frffkk*fttsttlck*efi****
****siirkntrigk*krtcl*tfkssttixiv*rfrikrssfr---

---EVINVIYDQISIYLTMFEKHPTWTKFKIQNXFKIICF*nckcyyfiys*r*ni*kyy
ssinfrrlsic*rwfsifiyfsfrfnfikslgnysmvifqflilhlqvrkgigiki*kk*
ki*kki*knke

Frame B:
flccvfkiffpdlniyenikitkq**eyk*ikk*kkk*KMAGDDDRNSIPMEHYDQQELX
PPQQELQQPPQQQQQQQQELQQQQQQQQQXYPSINDLDSSRNNSPQVPHYASKNSFNNNN
SSSNQSLEKIQELESKKELAYEHLSPAPQSXSYEDLGLKDQVLE---

---kl*m*fmikfqfi*qclkniqlgqnlkfkxtlklfvfkivnviilyilrdrifenit
hqsilegcpfvdvgsqflfilvldltlsnlweiirw*yssf*ffifkygkelvlkskknk
kykkkykkikk

Frame C:
fcvvclkfffqilifmki*k*qnsnknink*knkkknkkwqvmmieiqfqwnimtnknyx
hhnknynnhhnnnnnnnknynnnnnnnnkxiqv*mi*ilqeiihhkyhimqvrihliiii
vvvinh*kkyknwkvkknlpmni*vqhhnhxrmki*d*kikf*---

---sykcnl*snfnlfnnv*ktsnldki*nskxl*nylflkl*mllfyifleieylkill
inqf*kvvhlltlvlnfylf*f*i*lyqifgklfdgdipvsdsssssternwy*nlkkik
nikknikk*r
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF355 (AFF355Q) /CSM/AF/AFF3-C/AFF355Q.Seq.d/                        821   0.0
SSG107 (SSG107Q) /CSM/SS/SSG1-A/SSG107Q.Seq.d/                        434   e-120
CFF444 (CFF444Q) /CSM/CF/CFF4-B/CFF444Q.Seq.d/                        412   e-114
FC-IC0818 (FC-IC0818Q) /CSM/FC-IC/FC-IC0818Q.Seq.d/                   335   7e-91
SSM120 (SSM120Q) /CSM/SS/SSM1-A/SSM120Q.Seq.d/                        184   2e-45
VSG390 (VSG390Q) /CSM/VS/VSG3-D/VSG390Q.Seq.d/                         50   6e-05
SHK316 (SHK316Q) /CSM/SH/SHK3-A/SHK316Q.Seq.d/                         50   6e-05
SFE765 (SFE765Q) /CSM/SF/SFE7-C/SFE765Q.Seq.d/                         50   6e-05
CHJ474 (CHJ474Q) /CSM/CH/CHJ4-D/CHJ474Q.Seq.d/                         50   6e-05
AFD187 (AFD187Q) /CSM/AF/AFD1-D/AFD187Q.Seq.d/                         50   6e-05
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           50   1e-09  14
BX842643|BX842643.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 2/4.    34   2e-09  19
AC115682|AC115682.2 Dictyostelium discoideum chromosome 2 map 4101105-4158643 strain AX4, complete sequence.    50   6e-09  10
AE014820|AE014820.1 Plasmodium falciparum 3D7 chromosome 14 section 5 of 13 of the complete sequence.    48   1e-08  11
AE014818|AE014818.1 Plasmodium falciparum 3D7 chromosome 14 section 3 of 13 of the complete sequence.    52   1e-08  11
AL049184|AL049184.5 Plasmodium falciparum DNA *** SEQUENCING IN PROGRESS *** from  MAL13P3.    48   2e-08  12
AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    44   2e-08  7
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    42   9e-08  12
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    46   1e-07  7
BX957236|BX957236.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    46   2e-07  8
dna update 2004. 3.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC148177_28(AC148177|pid:none) Medicago truncatula clone mth2-4c...    34   5.4
AC139746_13(AC139746|pid:none) Medicago truncatula clone mth2-17...    34   5.4
protein update 2008.11.19
PSORT 

 psg: 0.75  gvh: 0.11  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFF355 is cyt
5' end seq. ID AFF355F
5' end seq. 
>AFF355F.Seq
ATTTTTGTGTTGTGTGTTTAAAATTTTTTTTCCAGATCTTAATATTTATGAAAATATAAA
AATAACAAAACAGTAATAAGAATATAAATAAATAAAAAAATAAAAAAAAAAATAAAAAAT
GGCAGGTGATGATGATAGAAATTCAATTCCAATGGAACATTATGACCAACAAGAATTACN
ACCACCACAACAAGAATTACAACAACCACCACAACAACAACAACAACAACAACAAGAATT
ACAACAACAACAACAACAACAACAACAAATNTATCCAAGTATAAATGATTTAGATTCTTC
AAGAAATAATTCACCACAAGTACCACATTATGCAAGTAAGAATTCATTTAATAATAATAA
TAGTAGTAGTAATCAATCATTAGAAAAAATACAAGAATTGGAAAGTAAAAAAGAACTTGC
CTATGAACATTTAAGTCCAGCACCACAATCATNATCGTATGAAGATTTAGGATTAAAAGA
TCAAGTTTTAGAG----------
Length of 5' end seq. 493
3' end seq. ID AFF355Z
3' end seq. 
>AFF355Z.Seq
----------GAAGTTATAAATGTAATTTATGATCAAATTTCAATTTATTTAACAATGTT
TGAAAAACATCCAACTTGGACAAAATTTAAAATTCAAAATNACTTTAAAATTATTTGTTT
TTAAAATTGTAAATGTTATTATTTTATATATTCTTAGAGATAGAATATTTGAAAATATTA
CTCATCAATCAATTTTAGAAGGTTGTCCATTTGTTGACGTTGGTTCTCAATTTTTATTTA
TTTTAGTTTTAGATTTAACTTTATCAAATCTTTGGGAAATTATTCGATGGTGATATTCCA
GTTTCTGATTCTTCATCTTCAAGTACGGAAAGGAATTGGTATTAAAATCTAAAAAAAATA
AAAAATATAAAAAAAAATATAAAAAAATAAAGAAA
Length of 3' end seq. 385
Connected seq. ID AFF355P
Connected seq. 
>AFF355P.Seq
ATTTTTGTGTTGTGTGTTTAAAATTTTTTTTCCAGATCTTAATATTTATGAAAATATAAA
AATAACAAAACAGTAATAAGAATATAAATAAATAAAAAAATAAAAAAAAAAATAAAAAAT
GGCAGGTGATGATGATAGAAATTCAATTCCAATGGAACATTATGACCAACAAGAATTACN
ACCACCACAACAAGAATTACAACAACCACCACAACAACAACAACAACAACAACAAGAATT
ACAACAACAACAACAACAACAACAACAAATNTATCCAAGTATAAATGATTTAGATTCTTC
AAGAAATAATTCACCACAAGTACCACATTATGCAAGTAAGAATTCATTTAATAATAATAA
TAGTAGTAGTAATCAATCATTAGAAAAAATACAAGAATTGGAAAGTAAAAAAGAACTTGC
CTATGAACATTTAAGTCCAGCACCACAATCATNATCGTATGAAGATTTAGGATTAAAAGA
TCAAGTTTTAGAG----------GAAGTTATAAATGTAATTTATGATCAAATTTCAATTT
ATTTAACAATGTTTGAAAAACATCCAACTTGGACAAAATTTAAAATTCAAAATNACTTTA
AAATTATTTGTTTTTAAAATTGTAAATGTTATTATTTTATATATTCTTAGAGATAGAATA
TTTGAAAATATTACTCATCAATCAATTTTAGAAGGTTGTCCATTTGTTGACGTTGGTTCT
CAATTTTTATTTATTTTAGTTTTAGATTTAACTTTATCAAATCTTTGGGAAATTATTCGA
TGGTGATATTCCAGTTTCTGATTCTTCATCTTCAAGTACGGAAAGGAATTGGTATTAAAA
TCTAAAAAAAATAAAAAATATAAAAAAAAATATAAAAAAATAAAGAAA
Length of connected seq. 878
Full length Seq ID -
Full length Seq. -
Length of full length seq. -