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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB382
Library CH
(Link to library)
Clone ID CHB382
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHB382P
(Link to Original site)
Representative DNA sequence 
>CHB382 (CHB382Q) /CSM/CH/CHB3-D/CHB382Q.Seq.d/
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGGAGTTAAAGCCACCGCTGGTGATAC
TCATTTAXXXXXXXXXXCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGA
ACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACC
AGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTT
AAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGA
TAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAA
ACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGC
ATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGA
TAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAAC
CGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAAT
CATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTAT
GTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTT
TTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAATAATAAGTTAGTAAAATCAA
TAAAGCTTCTCATATATA
sequence update 2002.10.25
Translated Amino Acid sequence 
FDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLK
MKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLE
KKGSGEKNILIFDLGGGTFDVSLLTIEDGVFGVKATAGDTHL---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*k**vskinkas
hi
Translated Amino Acid sequence (All Frames) 
Frame A:
i*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr*n*sfltrrnlfngtlk
nernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**tncccyclwfr
kerfw*keyfnfrfrwwyi*cfspyh*rwciws*shrw*ysf---

---liinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*my
qlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsl
lkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*v
nsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflknnklvksikl
liy

Frame B:
FDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKGETKVFSPEEISSMVLLK
MKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAAAIAYGLE
KKGSGEKNILIFDLGGGTFDVSLLTIEDGVFGVKATAGDTHL---

---**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkci
s*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc
*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*
tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflkiis**nq*sf
syi

Frame C:
lmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklnskvklkfshqkkslqwys*k
*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqllllllmv*k
rkvlvkrif*fsi*vvvhlmflslplkmvylelkpplvili---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*k**vskinkas
hi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB382 (CHB382Q) /CSM/CH/CHB3-D/CHB382Q.Seq.d/                       2355   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1415   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1415   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1415   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1415   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1415   0.0
VHO677 (VHO677Q) /CSM/VH/VHO6-D/VHO677Q.Seq.d/                       1415   0.0
VHO566 (VHO566Q) /CSM/VH/VHO5-C/VHO566Q.Seq.d/                       1415   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       1415   0.0
VHK721 (VHK721Q) /CSM/VH/VHK7-A/VHK721Q.Seq.d/                       1415   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X75263|X75263.1 D.discoideum (AX3) mRNA for heat shock cognate protein (hsc70).  1354   0.0    2
L22736|L22736.1 Dictyostelium discoideum heat shock protein (hspB) gene,.  1346   0.0    2
AF025951|AF025951.1 Dictyostelium discoideum heat-shock cognate protein 70 (hsc70) mRNA, complete cds.   293   e-145  7
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   293   e-143  11
A58313|A58313.1 Sequence 1 from Patent WO9636707.                 133   e-115  10
AX536934|AX536934.1 Sequence 535 from Patent WO02064766.          133   e-115  10
Z30210|Z30210.1 C.albicans (ATCC 20955) cahsp70 mRNA for heat shock protein 70.   133   e-115  10
AF221542|AF221542.1 Cryptosporidium muris isolate 589 heat shock protein 70 (HSP70) gene, partial cds.   125   4e-99  9
M19753|M19753.1 Plasmodium falciparum heat shock protein (HSP70) homologue mRNA, complete cds.    78   3e-97  13
AF221543|AF221543.1 Cryptosporidium muris isolate 707 heat shock protein 70 (HSP70) gene, partial cds.   133   5e-96  8
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   352   1e-95
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   352   1e-95
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   296   7e-79
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   295   3e-78
AY582833_1(AY582833|pid:none) Chytriomyces confervae strain CBS ...   278   2e-73
AY582835_1(AY582835|pid:none) Nuclearia simplex strain CCAP1552/...   273   1e-71
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   272   1e-71
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   272   1e-71
AY582832_1(AY582832|pid:none) Rhizophlyctis rosea strain CBS 576...   271   2e-71
EU679414_1(EU679414|pid:none) Tetranychus cinnabarinus heat shoc...   271   3e-71
protein update 2009. 3.29
PSORT 

 psg: 0.52  gvh: 0.25  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHB382 is cyt
5' end seq. ID CHB382F
5' end seq. 
>CHB382F.Seq
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGGAGTTAAAGCCACCGCTGGTGATAC
TCATTTANNNNNNNNNN
Length of 5' end seq. 497
3' end seq. ID CHB382Z
3' end seq. 
>CHB382Z.Seq
NNNNNNNNNNCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCT
ATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCA
AGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTA
TCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGT
CGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAA
GATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACT
GTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCA
ACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAA
AAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGT
AAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAAT
GATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAG
GAAAACATCTCTATCAAATAGATTTCTTAAAAATAATAAGTTAGTAAAATCAATAAAGCT
TCTCATATATA
Length of 3' end seq. 731
Connected seq. ID CHB382P
Connected seq. 
>CHB382P.Seq
ATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATAT
GAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGA
ATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAA
AATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGT
TCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAA
ATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGA
AAAGAAAGGTTCTGGTGAAAAGAATATTTTAATTTTCGATTTAGGTGGTGGTACATTTGA
TGTTTCTCTCCTTACCATTGAAGATGGTGTATTTGGAGTTAAAGCCACCGCTGGTGATAC
TCATTTA----------CTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGA
ACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACC
AGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTT
AAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGA
TAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAA
ACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGC
ATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGA
TAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAAC
CGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAAT
CATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTAT
GTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTT
TTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAATAATAAGTTAGTAAAATCAA
TAAAGCTTCTCATATATA
Length of connected seq. 1208
Full length Seq ID -
Full length Seq. -
Length of full length seq. -