>AFF350 (AFF350Q) /CSM/AF/AFF3-C/AFF350Q.Seq.d/
XXXXXXXXXXGTTAAAGGAATTTCAGAAGCTTTGGATAAATTTGAAAAAGAAACCGGACT
TGATATACCAATTCATGTCGATGCAGCAAGTGGTGGTTTCGTTGCACCCTTTCTTCAACC
AGAAATTGTTTGGGATTTTAGACTACCACGTGTTAAATCAATTAATGGCTCTGGTCATAA
ATTTGGTCTATCTCCATTGGGAGTTGGCTGGGTCGTATGGAGAGGTAAAAATGACATTCA
CAAGGATTTGGTATTCGATGTCAACTATCTTGGTGGCAATATGCCAACATTTTCATTGAA
TTTCTCACGTCCAGGTGGTCAAATAGTATGCCAGTACTATAACTTTTTGCGCCATGGTCG
TAGTGGATATACCGCAGTTCATCAAGCTTGTCTAGATCATGGTATTTTCATTTCCAAGCA
AGTTAAAAAAACACGGTATTTTTGATATTGTTTATGATGGTACTGGTGCACTTCCAGGTG
CATGTTGGAAATTGAAAAAAGATGCAAAAGTAAAATTCAATCTCTTTGACCTTTCAGATA
GAATGAGAAATAGAGGTTGGCAAATTGCATCCTATACTCTACCAAACTTACCAGATATTG
TAGTTCAAAGAGTATTAATTAGACATGGTTTCAGTCATGATATGGCAATGCTCTTAATNG
AAGATTTCAATCGCAGTATTGAATATTTTGATAAACAACCAAGGTTCAAAGTATTTCAAA
AGGATAAAGNTCAATCATTCCCATCANTAA

---KGISEALDKFEKETGLDIPIHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKF
GLSPLGVGWVVWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRS
GYTAVHQACLDHGIFISKQVKKTRYF*ycl*wywctsrcmleiekrckskiqsl*pfr*n
ek*rlancilystkltrycssksin*twfqs*ygnalnxrfqsqy*if**ttkvqsiskg
*xsiipix

Frame A:
---lkefqklwinlkkkpdliyqfmsmqqvvvslhpffnqklfgildyhvlnqlmalvin
lvylhwelagsygevkmtftriwysmstilvaicqhfh*ishvqvvk*yastitfcamvv
vdipqfiklv*imvfsfpsklkkhgifdivydgtgalpgacwklkkdakvkfnlfdlsdr
mrnrgwqiasytlpnlpdivvqrvlirhgfshdmamllxedfnrsieyfdkqprfkvfqk
dkxqsfpsx


Frame B:
---*rnfrsfg*i*krnrt*ytnscrcskwwfrctlsstrnclgf*tttc*in*wlws*i
wsisigswlgrmer*k*hsqgfgircqlswwqyanifiefltsrwsnsmpvl*lfapws*
wiyrsssslsrswyfhfqas*kntvflilfmmvlvhfqvhvgn*kkmqk*nsisltfqie
*eievgklhpilyqtyqil*fkey*ldmvsvmiwqcs*xkisiavlnilinnqgskyfkr
ikxnhshx*


Frame C:
---KGISEALDKFEKETGLDIPIHVDAASGGFVAPFLQPEIVWDFRLPRVKSINGSGHKF
GLSPLGVGWVVWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVCQYYNFLRHGRS
GYTAVHQACLDHGIFISKQVKKTRYF*ycl*wywctsrcmleiekrckskiqsl*pfr*n
ek*rlancilystkltrycssksin*twfqs*ygnalnxrfqsqy*if**ttkvqsiskg
*xsiipix

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF350 (AFF350Q) /CSM/AF/AFF3-C/AFF350Q.Seq.d/                       1407   0.0
SFG840 (SFG840Q) /CSM/SF/SFG8-B/SFG840Q.Seq.d/                       1340   0.0
SFD334 (SFD334Q) /CSM/SF/SFD3-B/SFD334Q.Seq.d/                       1338   0.0
SFD593 (SFD593Q) /CSM/SF/SFD5-D/SFD593Q.Seq.d/                       1219   0.0
CFJ364 (CFJ364Q) /CSM/CF/CFJ3-C/CFJ364Q.Seq.d/                       1217   0.0
CFJ215 (CFJ215Q) /CSM/CF/CFJ2-A/CFJ215Q.Seq.d/                       1217   0.0
SFE561 (SFE561Q) /CSM/SF/SFE5-C/SFE561Q.Seq.d/                       1205   0.0
SFG754 (SFG754Q) /CSM/SF/SFG7-C/SFG754Q.Seq.d/                       1193   0.0
SFC434 (SFC434Q) /CSM/SF/SFC4-B/SFC434Q.Seq.d/                       1193   0.0
CHB178 (CHB178Q) /CSM/CH/CHB1-D/CHB178Q.Seq.d/                       1193   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AR375678|AR375678.1 Sequence 684 from patent US 6605709.           56   1e-11  3
BG138757|BG138757.1 EST479199 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP11A1 5' sequence, mRNA sequence.    66   1e-09  2
CF811515|CF811515.1 NA716 cDNA non acclimated Bluecrop library Vaccinium corymbosum cDNA 5', mRNA sequence.    70   1e-08  2
AF352732|AF352732.1 Nicotiana tabacum glutamate decarboxylase isozyme 3 mRNA, complete cds.    58   2e-07  2
CK273593|CK273593.1 EST719671 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADC22 5' end, mRNA sequence.    50   2e-07  2
CD008053|CD008053.1 VVB082D05_335234 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB082D05 5, mRNA sequence.    64   6e-07  2
CD006712|CD006712.1 VVB066D06_332552 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB066D06 5, mRNA sequence.    64   6e-07  2
CD719214|CD719214.1 VVB158G03_410335 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB158G03 5, mRNA sequence.    64   7e-07  2
AW622257|AW622257.1 EST313055 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14P1 5', mRNA sequence.    66   8e-07  1
CA813481|CA813481.1 CA48LN10IF-E8 Cabernet Sauvignon Leaf - CA48LN Vitis vinifera cDNA clone CA48LN10IF-E8 5', mRNA sequence.    64   8e-07  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM178379_1197(FM178379|pid:none) Aliivibrio salmonicida LFI1238 ...   229   2e-86
CP000020_1061(CP000020|pid:none) Vibrio fischeri ES114 chromosom...   228   7e-85
CP000789_2953(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 ch...   222   2e-83
CP000851_2941(CP000851|pid:none) Shewanella pealeana ATCC 700345...   225   1e-82
CP001358_45(CP001358|pid:none) Desulfovibrio desulfuricans subsp...   219   3e-81
CR522870_385(CR522870|pid:none) Desulfotalea psychrophila LSv54 ...   212   3e-80
AM942759_1390(AM942759|pid:none) Proteus mirabilis strain HI4320...   213   9e-79
CP000439_1647(CP000439|pid:none) Francisella tularensis subsp. n...   217   2e-74
AM167904_2795(AM167904|pid:none) Bordetella avium 197N complete ...   205   1e-73
CP000608_56(CP000608|pid:none) Francisella tularensis subsp. tul...   214   1e-73

 psg: 0.75  gvh: 0.40  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFF350 is cyt

>AFF350Z.Seq
----------GTTAAAGGAATTTCAGAAGCTTTGGATAAATTTGAAAAAGAAACCGGACT
TGATATACCAATTCATGTCGATGCAGCAAGTGGTGGTTTCGTTGCACCCTTTCTTCAACC
AGAAATTGTTTGGGATTTTAGACTACCACGTGTTAAATCAATTAATGGCTCTGGTCATAA
ATTTGGTCTATCTCCATTGGGAGTTGGCTGGGTCGTATGGAGAGGTAAAAATGACATTCA
CAAGGATTTGGTATTCGATGTCAACTATCTTGGTGGCAATATGCCAACATTTTCATTGAA
TTTCTCACGTCCAGGTGGTCAAATAGTATGCCAGTACTATAACTTTTTGCGCCATGGTCG
TAGTGGATATACCGCAGTTCATCAAGCTTGTCTAGATCATGGTATTTTCATTTCCAAGCA
AGTTAAAAAAACACGGTATTTTTGATATTGTTTATGATGGTACTGGTGCACTTCCAGGTG
CATGTTGGAAATTGAAAAAAGATGCAAAAGTAAAATTCAATCTCTTTGACCTTTCAGATA
GAATGAGAAATAGAGGTTGGCAAATTGCATCCTATACTCTACCAAACTTACCAGATATTG
TAGTTCAAAGAGTATTAATTAGACATGGTTTCAGTCATGATATGGCAATGCTCTTAATNG
AAGATTTCAATCGCAGTATTGAATATTTTGATAAACAACCAAGGTTCAAAGTATTTCAAA
AGGATAAAGNTCAATCATTCCCATCANTAA