>AFF346 (AFF346Q) /CSM/AF/AFF3-B/AFF346Q.Seq.d/
ACATATATCAAACAAAATATGCATATCCCAGTTGTTGGAGAAACCCCAGATGATGCAGTA
AACCAATTAGCAGAAAAAGTTGAAAATGCTAATGTTGAAGATAAAGAAGGTGAAATTAAG
GCTTGTGTTGCAGAACCACATGATGGGGAAGTAACAAAAGGAAATGGAGAAGAAGAAGAA
CCAAAAATTAAAGAAGATTTAAAAGTTCAATTAGAACCAAAGGTTGAAAAAATTGAAACA
CCAAAGAATACAAATATTTTAGTTTTAGGTAGTACTGGTGTTGGTAAAAGTACATTTATT
TCATNATTGGTAAACTATTTGAAATATGAATCATTGGAACAAGCTATGAATCTTGCCAGT
TTGGATAATGTTGCCACCATTCCTAATAAAGTTCAAATTGTTGATTCGAATAATGTATCA
CATAGTTTCGTTGTTGGTAAATCAAAATACCTTTCACAAGAACATAAAGATGGTGAATCA
GTTACCGTTAAGCCAAAGAGATATGTCATCGATATGGGAAACAATAAAACAATGACAATT
TTAGATACACCAGGTGTTGCCGATACTCGTGGTTGTAAACAAGATGACTTAAAXXXXXXX
XXXTGGATACTCCAAAAGGTGCTAAAGAATTCGTTGGTCGTCATTGTTCCGCCTCCTTCA
AGAATAAAATCAATAAAAGTTCAAGTCTCTACTGGCATCACACATTTGGTATCTTGGGTG
GTTGTAAAGAATGTGGTTGTTCTTATAAAGATCATTCAGTGTCTGATATCGTTCATGTTA
AAGAAGCAATTCAAGTCATTGATGAAGGTATCAAAGGTAAAATCCTTTCCAAAGAAGAAC
TCACTGCTGTCGCCAATAGAAGAGTTAAAGAGAATTTAGAACTCAAAGAGAAATATTCCC
AAGAATTTGAGGTTTTATCAAATGCATTTGCCAAATTTGCAATCTTTGTAGCTCATAATT
CATTGGTTGCTGTTAATCACGACTATGCAGAATACCTCCAATTGGAAATTAAAAACCTTA
AAGAAAGCCCAAATGTTAAAGATAGAGAGAAACAATTAGAAAAATTAAATGAAATCCTTA
AATCTCATCAAATTGAACGTTGATACTTTTGAAGCCGCCCGTTCAAAAGAAAAAGAAGGT
CTTTCACCACACGATATAAATCTCCTTGGTGAAGAAGTTTTATCACTTGAAATCTCTGGT
AGAATCATTAAAGATATTCAAAGTCATAATGAAAAACAAAAATATGCTTCATCAAAAAAA
GTTAAAAATGTTAAAATTACATTAAATAAACAAGTTGAAAGTGCTATTAAAGGTTATTAA
ATTAAATTAAAATAA

TYIKQNMHIPVVGETPDDAVNQLAEKVENANVEDKEGEIKACVAEPHDGEVTKGNGEEEE
PKIKEDLKVQLEPKVEKIETPKNTNILVLGSTGVGKSTFISXLVNYLKYESLEQAMNLAS
LDNVATIPNKVQIVDSNNVSHSFVVGKSKYLSQEHKDGESVTVKPKRYVIDMGNNKTMTI
LDTPGVADTRGCKQDDL---

---DTPKGAKEFVGRHCSASFKNKINKSSSLYWHHTFGILGGCKECGCSYKDHSVSDIVH
VKEAIQVIDEGIKGKILSKEELTAVANRRVKENLELKEKYSQEFEVLSNAFAKFAIFVAH
NSLVAVNHDYAEYLQLEIKNLKESPNVKDREKQLEKLNEILKSHQIER*yf*srpfkrkr
rsfttrykspw*rsfit*nlw*nh*rysks**ktkicfikks*kc*nyik*ts*kcy*rl
ln*iki

Frame A:
TYIKQNMHIPVVGETPDDAVNQLAEKVENANVEDKEGEIKACVAEPHDGEVTKGNGEEEE
PKIKEDLKVQLEPKVEKIETPKNTNILVLGSTGVGKSTFISXLVNYLKYESLEQAMNLAS
LDNVATIPNKVQIVDSNNVSHSFVVGKSKYLSQEHKDGESVTVKPKRYVIDMGNNKTMTI
LDTPGVADTRGCKQDDL---

---wilqkvlknslvvivpppsriksikvqvstgithlvswvvvknvvvlikiiqclisf
mlkkqfkslmkvskvksfpkknsllspieelkri*nskrnipknlrfyqmhlpnlqsl*l
iihwlllittmqntsnwklktlkkaqmlkiernn*kn*mkslnliklnvdtfeaarskek
eglsphdinllgeevlsleisgriikdiqshnekqkyasskkvknvkitlnkqvesaikg
y*iklk*

Frame B:
hisnkicisqllekpqmmq*tn*qkklkmlmlkikkvklrlvlqnhmmgk*qkemekkkn
qklkki*kfn*nqrlkklkhqriqif*f*vvlvlvkvhlfhxw*ti*nmnhwnkl*ilpv
wimlppflikfkllirimyhivsllvnqntfhknikmvnqlplsqrdmssiwetikq*qf
*ihqvlpilvvvnkmt*---

---gyskrc*rirwsslfrllqe*nq*kfksllashiwylgwl*rmwlfl*rsfsv*yrs
c*rsnssh**ryqr*npfqrrthccrq*ks*refrtqreifpri*gfikcicqicnlcss
*figcc*srlcrippign*kp*rkpkc*r*retirkik*np*issn*tlillkppvqkkk
kvfhhti*islvkkfyhlkslveslkifkvimknknmlhqkklkmlklh*inklkvllkv
ikln*n

Frame C:
iyqtkyaypscwrnpr*cskpisrks*kc*c*r*rr*n*glccrtt*wgsnkrkwrrrrt
kn*rrfkssirtkg*kn*ntkeykyfsfr*ywcw*kyiyfxigklfei*iigtsyescqf
g*cchhs**ssnc*fe*cit*frcw*ikipftrt*rw*isyr*akeichrygkq*nndnf
rytrccryswl*tr*lk---

---DTPKGAKEFVGRHCSASFKNKINKSSSLYWHHTFGILGGCKECGCSYKDHSVSDIVH
VKEAIQVIDEGIKGKILSKEELTAVANRRVKENLELKEKYSQEFEVLSNAFAKFAIFVAH
NSLVAVNHDYAEYLQLEIKNLKESPNVKDREKQLEKLNEILKSHQIER*yf*srpfkrkr
rsfttrykspw*rsfit*nlw*nh*rysks**ktkicfikks*kc*nyik*ts*kcy*rl
ln*iki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF346 (AFF346Q) /CSM/AF/AFF3-B/AFF346Q.Seq.d/                       2375   0.0
SFK866 (SFK866Q) /CSM/SF/SFK8-C/SFK866Q.Seq.d/                       1229   0.0
SFG571 (SFG571Q) /CSM/SF/SFG5-C/SFG571Q.Seq.d/                       1229   0.0
AHA602 (AHA602Q) /CSM/AH/AHA6-A/AHA602Q.Seq.d/                       1229   0.0
AFO655 (AFO655Q) /CSM/AF/AFO6-C/AFO655Q.Seq.d/                       1229   0.0
AFO362 (AFO362Q) /CSM/AF/AFO3-C/AFO362Q.Seq.d/                       1229   0.0
AFM727 (AFM727Q) /CSM/AF/AFM7-B/AFM727Q.Seq.d/                       1229   0.0
AFE881 (AFE881Q) /CSM/AF/AFE8-D/AFE881Q.Seq.d/                       1229   0.0
AFC368 (AFC368Q) /CSM/AF/AFC3-C/AFC368Q.Seq.d/                       1229   0.0
AFL362 (AFL362Q) /CSM/AF/AFL3-C/AFL362Q.Seq.d/                       1227   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116979|AC116979.3 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   6e-08  22
AL445564|AL445564.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 2/3.    30   8e-08  21
AP004173|AP004173.1 Mycoplasma penetrans genomic DNA, complete sequence, section 4/5.    38   1e-07  14
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   1e-07  16
AR271569|AR271569.1 Sequence 1 from patent US 6503729.             34   4e-07  27
AP003515|AP003515.1 Clostridium perfringens plasmid pCP13 DNA, complete sequence.    38   7e-06  9
AL445565|AL445565.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 3/3.    34   9e-06  21
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    32   1e-05  28
AC005140|AC005140.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    38   1e-05  15
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  36   1e-05  16

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB120174_1(AB120174|pid:none) Dictyostelium discoideum DD8-14 mR...   379   e-103
AC114263_3(AC114263|pid:none) Dictyostelium discoideum chromosom...    82   5e-14
BC160745_1(BC160745|pid:none) Xenopus laevis hypothetical protei...    44   0.016
(P0C1J6) RecName: Full=FK506-binding protein 4;          EC=5.2....    42   0.036
BC155597_1(BC155597|pid:none) Danio rerio zgc:172075, mRNA (cDNA...    42   0.036
BC152666_1(BC152666|pid:none) Danio rerio hypothetical LOC554469...    42   0.062
CR954211_349(CR954211|pid:none) Ostreococcus tauri strain OTTH05...    41   0.11
AC147498_13(AC147498|pid:none) Medicago truncatula clone mth2-6f...    41   0.11
CP000619_85(CP000619|pid:none) Burkholderia vietnamiensis G4 pla...    41   0.11
AL390354_21(AL390354|pid:none) Neurospora crassa DNA linkage gro...    40   0.14

 psg: 0.80  gvh: 0.16  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: Golgi

>> prediction for AFF346 is nuc

>AFF346F.Seq
ACATATATCAAACAAAATATGCATATCCCAGTTGTTGGAGAAACCCCAGATGATGCAGTA
AACCAATTAGCAGAAAAAGTTGAAAATGCTAATGTTGAAGATAAAGAAGGTGAAATTAAG
GCTTGTGTTGCAGAACCACATGATGGGGAAGTAACAAAAGGAAATGGAGAAGAAGAAGAA
CCAAAAATTAAAGAAGATTTAAAAGTTCAATTAGAACCAAAGGTTGAAAAAATTGAAACA
CCAAAGAATACAAATATTTTAGTTTTAGGTAGTACTGGTGTTGGTAAAAGTACATTTATT
TCATNATTGGTAAACTATTTGAAATATGAATCATTGGAACAAGCTATGAATCTTGCCAGT
TTGGATAATGTTGCCACCATTCCTAATAAAGTTCAAATTGTTGATTCGAATAATGTATCA
CATAGTTTCGTTGTTGGTAAATCAAAATACCTTTCACAAGAACATAAAGATGGTGAATCA
GTTACCGTTAAGCCAAAGAGATATGTCATCGATATGGGAAACAATAAAACAATGACAATT
TTAGATACACCAGGTGTTGCCGATACTCGTGGTTGTAAACAAGATGACTTAAA-------
---

>AFF346Z.Seq
----------TGGATACTCCAAAAGGTGCTAAAGAATTCGTTGGTCGTCATTGTTCCGCC
TCCTTCAAGAATAAAATCAATAAAAGTTCAAGTCTCTACTGGCATCACACATTTGGTATC
TTGGGTGGTTGTAAAGAATGTGGTTGTTCTTATAAAGATCATTCAGTGTCTGATATCGTT
CATGTTAAAGAAGCAATTCAAGTCATTGATGAAGGTATCAAAGGTAAAATCCTTTCCAAA
GAAGAACTCACTGCTGTCGCCAATAGAAGAGTTAAAGAGAATTTAGAACTCAAAGAGAAA
TATTCCCAAGAATTTGAGGTTTTATCAAATGCATTTGCCAAATTTGCAATCTTTGTAGCT
CATAATTCATTGGTTGCTGTTAATCACGACTATGCAGAATACCTCCAATTGGAAATTAAA
AACCTTAAAGAAAGCCCAAATGTTAAAGATAGAGAGAAACAATTAGAAAAATTAAATGAA
ATCCTTAAATCTCATCAAATTGAACGTTGATACTTTTGAAGCCGCCCGTTCAAAAGAAAA
AGAAGGTCTTTCACCACACGATATAAATCTCCTTGGTGAAGAAGTTTTATCACTTGAAAT
CTCTGGTAGAATCATTAAAGATATTCAAAGTCATAATGAAAAACAAAAATATGCTTCATC
AAAAAAAGTTAAAAATGTTAAAATTACATTAAATAAACAAGTTGAAAGTGCTATTAAAGG
TTATTAAATTAAATTAAAATAA

>AFF346P.Seq
ACATATATCAAACAAAATATGCATATCCCAGTTGTTGGAGAAACCCCAGATGATGCAGTA
AACCAATTAGCAGAAAAAGTTGAAAATGCTAATGTTGAAGATAAAGAAGGTGAAATTAAG
GCTTGTGTTGCAGAACCACATGATGGGGAAGTAACAAAAGGAAATGGAGAAGAAGAAGAA
CCAAAAATTAAAGAAGATTTAAAAGTTCAATTAGAACCAAAGGTTGAAAAAATTGAAACA
CCAAAGAATACAAATATTTTAGTTTTAGGTAGTACTGGTGTTGGTAAAAGTACATTTATT
TCATNATTGGTAAACTATTTGAAATATGAATCATTGGAACAAGCTATGAATCTTGCCAGT
TTGGATAATGTTGCCACCATTCCTAATAAAGTTCAAATTGTTGATTCGAATAATGTATCA
CATAGTTTCGTTGTTGGTAAATCAAAATACCTTTCACAAGAACATAAAGATGGTGAATCA
GTTACCGTTAAGCCAAAGAGATATGTCATCGATATGGGAAACAATAAAACAATGACAATT
TTAGATACACCAGGTGTTGCCGATACTCGTGGTTGTAAACAAGATGACTTAAA-------
---TGGATACTCCAAAAGGTGCTAAAGAATTCGTTGGTCGTCATTGTTCCGCCTCCTTCA
AGAATAAAATCAATAAAAGTTCAAGTCTCTACTGGCATCACACATTTGGTATCTTGGGTG
GTTGTAAAGAATGTGGTTGTTCTTATAAAGATCATTCAGTGTCTGATATCGTTCATGTTA
AAGAAGCAATTCAAGTCATTGATGAAGGTATCAAAGGTAAAATCCTTTCCAAAGAAGAAC
TCACTGCTGTCGCCAATAGAAGAGTTAAAGAGAATTTAGAACTCAAAGAGAAATATTCCC
AAGAATTTGAGGTTTTATCAAATGCATTTGCCAAATTTGCAATCTTTGTAGCTCATAATT
CATTGGTTGCTGTTAATCACGACTATGCAGAATACCTCCAATTGGAAATTAAAAACCTTA
AAGAAAGCCCAAATGTTAAAGATAGAGAGAAACAATTAGAAAAATTAAATGAAATCCTTA
AATCTCATCAAATTGAACGTTGATACTTTTGAAGCCGCCCGTTCAAAAGAAAAAGAAGGT
CTTTCACCACACGATATAAATCTCCTTGGTGAAGAAGTTTTATCACTTGAAATCTCTGGT
AGAATCATTAAAGATATTCAAAGTCATAATGAAAAACAAAAATATGCTTCATCAAAAAAA
GTTAAAAATGTTAAAATTACATTAAATAAACAAGTTGAAAGTGCTATTAAAGGTTATTAA
ATTAAATTAAAATAA