NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB245
Library CH
(Link to library)
Clone ID CHB245
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16455-1
Original site URL
Representative seq. ID CHB245P
(Link to Original site)
Representative DNA sequence 
>CHB245 (CHB245Q) /CSM/CH/CHB2-B/CHB245Q.Seq.d/
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GCXXXXXXXXXXACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAANAAATTAAAA
NAGGTATGGTCGCTGGTGACTCCAAAAACGATCCNCCNCAANAAACTGAAAAATTCGTTG
NTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTNTTAN
ATTGTCACNCTGCTCACATTGCCTGTAAATTCNCTGAAATCGTCGATAAAGTTGATCGTC
GNACTGGNGCCGTCGTTGCCAAANAAGGNACTGCCNCCGTCGTCTTAAAGAATGGTGATG
CTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCNCTGAANACC
CNCCATTAGGTCGTTTCNCCGTCANANANATGAGACAAACCGTTGCCGTCGGTGTCATCA
AATCAACCGTCAANAAAGCCCCAGGTAAAGCAGGTGATAANAAAGGTGCTGCTGCTCCAT
CAAAGAAGAAATNAATAATTTAAAAAAAATTTTATGATTATTCTAAAAATAAAAAAAAAA
ATAATAAAAAAGAGCTTTTAA
sequence update 2002.10.25
Translated Amino Acid sequence 
kfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIV---

---NVGFNVKNVSVKXIKXGMVAGDSKNDPPQXTEKFVXQVIVLNHPGQIHAGYSPVLXC
HXAHIACKFXEIVDKVDRRTGAVVAKXGTAXVVLKNGDAAMVELTPSRPMCVESFXEXPP
LGRFXVXXMRQTVAVGVIKSTVXKAPGKAGDXKGAAAPSKKKXII*kkfydysknkkknn
kkell
Translated Amino Acid sequence (All Frames) 
Frame A:
kfisqkv*YKMGKEKTHINIVVIGHVDAGKSTTTGHLIYKCGGIDKRVIEKYEKEASEMG
KQSFKYAWVMDKLKAERERGITIDIALWKFETSKYYFTIIDAPGHRDFIKNMITGTSQAD
CAVLVIASPTGEFEAGIAKNGQTREHALLAYTLGVKQMIV---

---tm*vstlktfqsxklkxvwslvtpktixxxklknslxklssstiqvksmlvthqxxi
vtlltlpvnslkssiklivxlxpslpxkxlppss*rmvmllwsn*phlvqcvlnhslxtx
h*vvspsxx*dkplpsvssnqpsxkpqvkqvixkvlllhqrrnx*fkknfmiilkikkki
ikksf*

Frame B:
nlfhkkyntrwvkkkhilissllvt*mlvnqpplvi*stnvvvsikelskntkrkllkwv
nnhsnmlglwin*klnvkevlpsillygnskpqnttspllmlqvteissri*llvphkpi
vls*slppqlvssklvlprtvklvntlslltl*vsnk*sl---

---qcrfqr*krfsqxn*xrygrw*lqkrsxxxn*kirxssyrpqpsrsnpcwlltsxxl
sxcshcl*ix*nrr*s*ssxwxrrcqxrxcxrrlkew*ccygrinpissnvc*iix*xpx
irsfxrxxxetnrcrrchqinrqxspr*sr**xrcccsikeexnnlkkil*lf*k*kkk*
*kraf

Frame C:
iyftksiiqdg*rknty*yrrywsrrcw*inhhwsfnlqmwwyr*ksyrkirkgsf*ng*
tiiqiclgyg*iks*t*kryyhrycfmeirnlkillhhy*csrsqrfhqeydywyltsrl
cclshclpnw*vrswycqersns*trspclhfrcqtndrc---

---NVGFNVKNVSVKXIKXGMVAGDSKNDPPQXTEKFVXQVIVLNHPGQIHAGYSPVLXC
HXAHIACKFXEIVDKVDRRTGAVVAKXGTAXVVLKNGDAAMVELTPSRPMCVESFXEXPP
LGRFXVXXMRQTVAVGVIKSTVXKAPGKAGDXKGAAAPSKKKXII*kkfydysknkkknn
kkell
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFO750 (VFO750Q) /CSM/VF/VFO7-C/VFO750Q.Seq.d/                        955   0.0
VFO682 (VFO682Q) /CSM/VF/VFO6-D/VFO682Q.Seq.d/                        955   0.0
VFN443 (VFN443Q) /CSM/VF/VFN4-B/VFN443Q.Seq.d/                        955   0.0
VFM669 (VFM669Q) /CSM/VF/VFM6-C/VFM669Q.Seq.d/                        955   0.0
VFM610 (VFM610Q) /CSM/VF/VFM6-A/VFM610Q.Seq.d/                        955   0.0
VFM513 (VFM513Q) /CSM/VF/VFM5-A/VFM513Q.Seq.d/                        955   0.0
VFM279 (VFM279Q) /CSM/VF/VFM2-D/VFM279Q.Seq.d/                        955   0.0
VFL419 (VFL419Q) /CSM/VF/VFL4-A/VFL419Q.Seq.d/                        955   0.0
VFK107 (VFK107Q) /CSM/VF/VFK1-A/VFK107Q.Seq.d/                        955   0.0
VFH468 (VFH468Q) /CSM/VF/VFH4-C/VFH468Q.Seq.d/                        955   0.0
own update 2002.11.20
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.   878   0.0    2
X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.   747   0.0    2
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   613   0.0    3
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   260   e-123  8
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   236   e-116  8
AF402092|AF402092.1 Pichia anomala strain NRRL Y-366 translation elongation factor 1-alpha gene, partial cds.   224   4e-98  6
AF402018|AF402018.1 Saccharomyces unisporus strain NRRL Y-1556 translation elongation factor 1-alpha gene, partial cds.   109   5e-95  7
AF402023|AF402023.1 Kluyveromyces piceae strain NRRL Y-17977 translation elongation factor 1-alpha gene, partial cds.   204   2e-91  4
CO483664|CO483664.1 GQ0206.B3_A14 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0206_A14 5', mRNA sequence.   319   3e-89  3
AF402020|AF402020.1 Saccharomyces transvaalensis strain NRRL Y-17245 translation elongation factor 1-alpha gene, partial cds.   121   1e-88  7
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   306   9e-82
X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   300   7e-80
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   300   7e-80
AB245432_1(AB245432|pid:none) Pocillopora damicornis EF1-alpha m...   283   5e-75
DQ440206_1(DQ440206|pid:none) Aedes aegypti clone AET-205 transl...   283   9e-75
(P29520) RecName: Full=Elongation factor 1-alpha;          Short...   283   9e-75
(Q90835) RecName: Full=Elongation factor 1-alpha 1;          Sho...   281   2e-74
AK167843_1(AK167843|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   281   2e-74
EU016386_1(EU016386|pid:none) Trichoplusia ni clone L608 elongat...   281   2e-74
AB174733_1(AB174733|pid:none) Macaca fascicularis brain cDNA clo...   281   2e-74
protein update 2009. 3.29
PSORT 

 psg: 0.84  gvh: 0.34  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for CHB245 is nuc
5' end seq. ID CHB245F
5' end seq. 
>CHB245F.Seq
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GCNNNNNNNNNN
Length of 5' end seq. 492
3' end seq. ID CHB245Z
3' end seq. 
>CHB245Z.Seq
NNNNNNNNNNACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAANAAATTAAAANA
GGTATGGTCGCTGGTGACTCCAAAAACGATCCNCCNCAANAAACTGAAAAATTCGTTGNT
CAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTNTTANAT
TGTCACNCTGCTCACATTGCCTGTAAATTCNCTGAAATCGTCGATAAAGTTGATCGTCGN
ACTGGNGCCGTCGTTGCCAAANAAGGNACTGCCNCCGTCGTCTTAAAGAATGGTGATGCT
GCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCNCTGAANACCCN
CCATTAGGTCGTTTCNCCGTCANANANATGAGACAAACCGTTGCCGTCGGTGTCATCAAA
TCAACCGTCAANAAAGCCCCAGGTAAAGCAGGTGATAANAAAGGTGCTGCTGCTCCATCA
AAGAAGAAATNAATAATTTAAAAAAAATTTTATGATTATTCTAAAAATAAAAAAAAAAAT
AATAAAAAAGAGCTTTTAA
Length of 3' end seq. 559
Connected seq. ID CHB245P
Connected seq. 
>CHB245P.Seq
AAATTTATTTCACAAAAAGTATAATACAAGATGGGTAAAGAAAAAACACATATTAATATC
GTCGTTATTGGTCACGTAGATGCTGGTAAATCAACCACCACTGGTCATTTAATCTACAAA
TGTGGTGGTATCGATAAAAGAGTTATCGAAAAATACGAAAAGGAAGCTTCTGAAATGGGT
AAACAATCATTCAAATATGCTTGGGTTATGGATAAATTAAAAGCTGAACGTGAAAGAGGT
ATTACCATCGATATTGCTTTATGGAAATTCGAAACCTCAAAATACTACTTCACCATTATT
GATGCTCCAGGTCACAGAGATTTCATCAAGAATATGATTACTGGTACCTCACAAGCCGAT
TGTGCTGTCTTAGTCATTGCCTCCCCAACTGGTGAGTTCGAAGCTGGTATTGCCAAGAAC
GGTCAAACTCGTGAACACGCTCTCCTTGCTTACACTTTAGGTGTCAAACAAATGATCGTT
GC----------ACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAANAAATTAAAA
NAGGTATGGTCGCTGGTGACTCCAAAAACGATCCNCCNCAANAAACTGAAAAATTCGTTG
NTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTNTTAN
ATTGTCACNCTGCTCACATTGCCTGTAAATTCNCTGAAATCGTCGATAAAGTTGATCGTC
GNACTGGNGCCGTCGTTGCCAAANAAGGNACTGCCNCCGTCGTCTTAAAGAATGGTGATG
CTGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCATTCNCTGAANACC
CNCCATTAGGTCGTTTCNCCGTCANANANATGAGACAAACCGTTGCCGTCGGTGTCATCA
AATCAACCGTCAANAAAGCCCCAGGTAAAGCAGGTGATAANAAAGGTGCTGCTGCTCCAT
CAAAGAAGAAATNAATAATTTAAAAAAAATTTTATGATTATTCTAAAAATAAAAAAAAAA
ATAATAAAAAAGAGCTTTTAA
Length of connected seq. 1031
Full length Seq ID -
Full length Seq. -
Length of full length seq. -