>CHB223 (CHB223Q) /CSM/CH/CHB2-A/CHB223Q.Seq.d/
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTAXXXX
XXXXXXCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCT
CTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAA
TCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAG
GTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGG
GTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAG
TTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTT
TTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAG
CCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAG
CTTTTGCTTCTCAAGCACTTTACAGTTGTAAGGCTTTGGGTTTGGATATGAACAAAGTTA
ATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAA
TGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGT
GTAT

FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGL---

---ENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDE
GIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVA
FAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQALYSCKALGLDMNKV
NPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVISMC

Frame A:
ifhiyi*yytp*likchsiqelklflvt*aldqf*nqiqlldqlnqemil*llhhfvqqf
vklkeehskrqhqmiy*hqllnilsklpn*iqn*lemllweqyyqdhhkvqpklelphy*
qvyqrkyhviqstdnvhqvykqlpivqqqskqvsmtlv---

---lkmsqsvlvspektkmnsl*ihtrrqvppkkqvnltmksfqsp*rprmakpsssikt
kvsvrkpplqiwpn*nqlskrvvqpplvtlvk*vmvpplv*l*kevppkdwvsrlnwfs*
llllsvsnhplwvlvqplqfqpp*nklvsplkistflksmkllllkhftvvrlwvwi*tk
liqmalvsl*vih*vllvpvkwlpfqenyivvkvnmv*slcv

Frame B:
ffifiynitphn**nviqfkn*nyfsslkhwtnfriksnfwis*irr*ycncctisysnl
*s*krsiqrdstr*fistsy*tyyqsyqirskinwrccygssitkiitrcnrs*schiis
rftkgstmlysqqtmfirftsncqlcsssqsrfl*hwf---

---*kcrrafwchqkrpr*ilckftqegrcrpksr*i*r*nrsshrkdrgwqnhrrr*rr
ryp*enhrcrfgqiktsfqrgwynhrw*l*sne*wcrrlfsyekkyrrkigsqg*igfrs
fcccrcrtihygywssrcnssrlktswshh*ryrrf*nq*sfcfsstlql*gfgfgyeqs
*skwlwyrfrssircywcpsngyhfkritss*r*iwcnlyvy

Frame C:
FSYLYIILHPITNKMSFNSRIETISRHLSTGPILESNPTSGSVKSGDDIVIVAPFRTAIC
KAKRGAFKETAPDDLLAPVIKHIIKVTKLDPKLIGDVVMGAVLPRSSQGATEVRVATLLA
GLPKEVPCYTVNRQCSSGLQAIANCAAAVKAGFYDIGL---

---ENVAERFGVTRKDQDEFSVNSHKKAGAAQKAGKFDDEIVPVTVKTEDGKTIVVDKDE
GIREKTTVADLAKLKPAFKEGGTTTAGNSSQMSDGAAACLVMKRSTAERLGLKVELVFVA
FAAVGVEPSIMGIGPAVAIPAALKQAGLTTKDIDVFEINEAFASQALYSCKALGLDMNKV
NPNGSGIALGHPLGATGARQMATISRELHRRKGKYGVISMC

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB223 (CHB223Q) /CSM/CH/CHB2-A/CHB223Q.Seq.d/                       2208   0.0
VFE239 (VFE239Q) /CSM/VF/VFE2-B/VFE239Q.Seq.d/                       1289   0.0
SLG762 (SLG762Q) /CSM/SL/SLG7-C/SLG762Q.Seq.d/                       1289   0.0
SFI740 (SFI740Q) /CSM/SF/SFI7-B/SFI740Q.Seq.d/                       1289   0.0
SFH501 (SFH501Q) /CSM/SF/SFH5-A/SFH501Q.Seq.d/                       1289   0.0
SFG758 (SFG758Q) /CSM/SF/SFG7-C/SFG758Q.Seq.d/                       1289   0.0
SFG482 (SFG482Q) /CSM/SF/SFG4-D/SFG482Q.Seq.d/                       1289   0.0
SFA524 (SFA524Q) /CSM/SF/SFA5-A/SFA524Q.Seq.d/                       1289   0.0
CHB394 (CHB394Q) /CSM/CH/CHB3-D/CHB394Q.Seq.d/                       1289   0.0
CFF662 (CFF662Q) /CSM/CF/CFF6-C/CFF662Q.Seq.d/                       1289   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1289   0.0    9
CN604220|CN604220.1 USDA_FP_131320 Vitis shuttleworthii L., grape Vitis shuttleworthii cDNA clone WHVs010_C03 5', mRNA sequence.    60   9e-12  3
CB005315|CB005315.1 VVC011E10_126660 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC011E10 5, mRNA sequence.    60   5e-11  4
AU089542|AU089542.1 Lotus japonicus flower bud cDNA clone: Ljfb-v09.    44   3e-10  4
CA917334|CA917334.1 EST641481 GPOD Medicago truncatula cDNA clone GPOD-35H18, mRNA sequence.    38   6e-10  5
CB008288|CB008288.1 VVC055H06_148036 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVC055H06 5, mRNA sequence.    60   2e-09  3
CD007738|CD007738.1 VVB078G11_334604 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB078G11 5, mRNA sequence.    60   2e-08  2
BM065622|BM065622.1 KS07005E12 KS07 Capsicum annuum cDNA, mRNA sequence.    68   3e-08  2
CA515447|CA515447.1 KS09042A06 KS09 Capsicum annuum cDNA, mRNA sequence.    68   3e-08  2
CD716621|CD716621.1 VVB129E11_405541 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB129E11 5, mRNA sequence.    60   3e-08  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116986_75(AC116986|pid:none) Dictyostelium discoideum chromoso...   657   0.0
X75329_1(X75329|pid:none) M.indica (Manila) THMF5 mRNA for 3-ket...   370   e-101
AF062589_2(AF062589|pid:none) Arabidopsis thaliana 3-keto-acyl-C...   369   e-101
AK230209_1(AK230209|pid:none) Arabidopsis thaliana mRNA for pero...   369   e-100
AM474896_9(AM474896|pid:none) Vitis vinifera contig VV79X001698....   369   e-100
S57792(S57792)acetyl-CoA C-acyltransferase (EC 2.3.1.16) B precu...   368   e-100
AC126019_22(AC126019|pid:none) Medicago truncatula clone mth2-22...   367   e-100
AB333797_1(AB333797|pid:none) Glycine max mRNA for peroxisomal 3...   367   e-100
AY383736_1(AY383736|pid:none) Glycine max 3-ketoacyl-CoA thiolas...   366   e-100
EU964831_1(EU964831|pid:none) Zea mays clone 281881 3-ketoacyl-C...   365   1e-99

 psg: 0.91  gvh: 0.40  alm: 0.28  top: 0.33  tms: 0.07  mit: 0.33  mip: 0.05
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: endoplasmic reticulum
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for CHB223 is nuc

>CHB223F.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTANNNN
NNNNNN

>CHB223Z.Seq
NNNNNNNNNNCTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAA
TTCTCTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGAT
GAAATCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGAC
GAAGGTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAA
GAGGGTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGT
TTAGTTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTA
GCTTTTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATT
CCAGCCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAAT
GAAGCTTTTGCTTCTCAAGCACTTTACAGTTGTAAGGCTTTGGGTTTGGATATGAACAAA
GTTAATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGT
CAAATGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCT
ATGTGTAT

>CHB223P.Seq
ATTTTTCATATTTATATATAATATTACACCCCATAACTAATAAAATGTCATTCAATTCAA
GAATTGAAACTATTTCTCGTCACTTAAGCACTGGACCAATTTTAGAATCAAATCCAACTT
CTGGATCAGTTAAATCAGGAGATGATATTGTAATTGTTGCACCATTTCGTACAGCAATTT
GTAAAGCTAAAAGAGGAGCATTCAAAGAGACAGCACCAGATGATTTATTAGCACCAGTTA
TTAAACATATTATCAAAGTTACCAAATTAGATCCAAAATTAATTGGAGATGTTGTTATGG
GAGCAGTATTACCAAGATCATCACAAGGTGCAACCGAAGTTAGAGTTGCCACATTATTAG
CAGGTTTACCAAAGGAAGTACCATGTTATACAGTCAACAGACAATGTTCATCAGGTTTAC
AAGCAATTGCCAATTGTGCAGCAGCAGTCAAAGCAGGTTTCTATGACATTGGTTTA----
------CTGAAAATGTCGCAGAGCGTTTTGGTGTCACCAGAAAAGACCAAGATGAATTCT
CTGTAAATTCACACAAGAAGGCAGGTGCCGCCCAAAAAGCAGGTAAATTTGACGATGAAA
TCGTTCCAGTCACCGTAAAGACCGAGGATGGCAAAACCATCGTCGTCGATAAAGACGAAG
GTATCCGTGAGAAAACCACCGTTGCAGATTTGGCCAAATTAAAACCAGCTTTCAAAGAGG
GTGGTACAACCACCGCTGGTAACTCTAGTCAAATGAGTGATGGTGCCGCCGCTTGTTTAG
TTATGAAAAGAAGTACCGCCGAAAGATTGGGTCTCAAGGTTGAATTGGTTTTCGTAGCTT
TTGCTGCTGTCGGTGTCGAACCATCCATTATGGGTATTGGTCCAGCCGTTGCAATTCCAG
CCGCCTTAAAACAAGCTGGTCTCACCACTAAAGATATCGACGTTTTTGAAATCAATGAAG
CTTTTGCTTCTCAAGCACTTTACAGTTGTAAGGCTTTGGGTTTGGATATGAACAAAGTTA
ATCCAAATGGCTCTGGTATCGCTTTAGGTCATCCATTAGGTGCTACTGGTGCCCGTCAAA
TGGCTACCATTTCAAGAGAATTACATCGTCGTAAAGGTAAATATGGTGTAATCTCTATGT
GTAT