>CHB186 (CHB186Q) /CSM/CH/CHB1-D/CHB186Q.Seq.d/
CACTGTTGGCCTACTGGTTTTTATTTATACACAATACATATAAAAGATGGCAGGTAAAGA
TTGGGAAATAGTACAAGAAAAAGCATTTACTGCATGGGTAAATTCAGTTTTAGATAAAAG
AGGTGAAAAGATTTCAGATGTCGGTAAAGACTTGAGTGATGGTGTCAAATTAATCTTCTT
TTTGGAACTCATCAGTXXXXXXXXXXTGGTACCACTGATGAACGTTCACTCGTTCTCTAC
ACATCTTTATTCTTCCATGCCTATAGAGCCAAGGAAGAGAAAGCTCGTTTAGAGAGTAGC
AAAAATGAAATGGCAAATCGTTTAGCCGGTTTAGAGAATTCATTGGAATCTGAAAAGGTC
TCACGTGAACAATTAATCAAACAAAAGGATCAACTCAATAGTTTATTGGCATCATTAGAG
TCCGAAGGTGCTGAACGTGAAAAGAGACTTCGTGAATTGGAAGCCAAACTCGATGAAACC
TTAAAGAACTTGGAATTAGAGAAATTGGCTCGTATGGAACTCGAAGCAAGATTAGCCAAG
ACCGAAAAGGATAGAGCTATCTTGGAATTGAAATTAGCTGAAGCCATCGATGAAAAATCA
AAACTCGAACAACAAATCGAAGCCACCAGAATCAGAGGTGCTGCTGAAGCTCAAGGTCTT
GGTCTTTTAAGAAAGAACCTCGACACTCACGTTCACGATTTACTCAAATGGCAAAAGTTA
ACAATGGAAAACTCTTCCTCATCCTCTATCGATGACCAAATCATCGTCGAAGTCTCTGGT
CTTCCATTTGGTGAACAAGTTAAACATTTAGCCACAAAATTAGAAGCAGAAAATTTAGCA
ATTATGAAACTTTTAAATCAAAAAGAAGANATTTAAAAGCTCAAAAATTAAAATCATCAA
AATCAAAAAAATAAATTAAAAAAA

tvgllvfiytqyi*KMAGKDWEIVQEKAFTAWVNSVLDKRGEKISDVGKDLSDGVKLIFF
LELIS---

---GTTDERSLVLYTSLFFHAYRAKEEKARLESSKNEMANRLAGLENSLESEKVSREQLI
KQKDQLNSLLASLESEGAEREKRLRELEAKLDETLKNLELEKLARMELEARLAKTEKDRA
ILELKLAEAIDEKSKLEQQIEATRIRGAAEAQGLGLLRKNLDTHVHDLLKWQKLTMENSS
SSSIDDQIIVEVSGLPFGEQVKHLATKLEAENLAIMKLLNQKEXI*klkn*nhqnqknkl
kk

Frame A:
hcwptgfylytihikdgr*rlgnstrksiycmgkfsfr*kr*kdfrcr*rle*wcqinll
fgthq---

---wyh**tftrslhifilpcl*sqgressfre*qk*ngksfsrfrefigi*kglt*tin
qtkgstq*figiirvrrc*t*kets*igsqtr*nlkelgireigsygtrskisqdrkg*s
ylgieis*shr*kiktrttnrshqnqrcc*ssrswsfkkeprhsrsrftqmakvnngklf
lilyr*pnhrrslwssiw*ts*tfshkirsrkfsnyetfkskrrxlkaqklksskskk*i
kk

Frame B:
tvgllvfiytqyi*KMAGKDWEIVQEKAFTAWVNSVLDKRGEKISDVGKDLSDGVKLIFF
LELIS---

---GTTDERSLVLYTSLFFHAYRAKEEKARLESSKNEMANRLAGLENSLESEKVSREQLI
KQKDQLNSLLASLESEGAEREKRLRELEAKLDETLKNLELEKLARMELEARLAKTEKDRA
ILELKLAEAIDEKSKLEQQIEATRIRGAAEAQGLGLLRKNLDTHVHDLLKWQKLTMENSS
SSSIDDQIIVEVSGLPFGEQVKHLATKLEAENLAIMKLLNQKEXI*klkn*nhqnqknkl
kk

Frame C:
llaywflfihntykrwqvkigk*ykkkhllhg*iqf*ikevkrfqmsvkt*vmvsn*ssf
wnss---

---vplmnvhsfsthlyssmpieprkrklv*rvakmkwqiv*pv*rihwnlkrshvnn*s
nkrinsivywhh*spkvlnvkrdfvnwkpnsmkp*rtwn*rnwlvwnskqd*prpkriel
swn*n*lkpsmknqnsnnkskppesevllklkvlvf*ertstltftiysngks*qwktlp
hplsmtkssskslvfhlvnklni*pqn*kqki*ql*nf*ikkkxfksskikiikikkin*
k

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB186 (CHB186Q) /CSM/CH/CHB1-D/CHB186Q.Seq.d/                       1667   0.0
SFH314 (SFH314Q) /CSM/SF/SFH3-A/SFH314Q.Seq.d/                       1310   0.0
AFH749 (AFH749Q) /CSM/AF/AFH7-C/AFH749Q.Seq.d/                       1310   0.0
SLE631 (SLE631Q) /CSM/SL/SLE6-B/SLE631Q.Seq.d/                       1306   0.0
SFB524 (SFB524Q) /CSM/SF/SFB5-A/SFB524Q.Seq.d/                       1302   0.0
VFD282 (VFD282Q) /CSM/VF/VFD2-D/VFD282Q.Seq.d/                       1289   0.0
AFJ320 (AFJ320Q) /CSM/AF/AFJ3-A/AFJ320Q.Seq.d/                       1287   0.0
AFD662 (AFD662Q) /CSM/AF/AFD6-C/AFD662Q.Seq.d/                       1279   0.0
VFJ634 (VFJ634Q) /CSM/VF/VFJ6-B/VFJ634Q.Seq.d/                       1275   0.0
VSJ276 (VSJ276Q) /CSM/VS/VSJ2-D/VSJ276Q.Seq.d/                       1243   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L49527|L49527.1 Dictyostelium discoideum cortexillin I mRNA, complete cds.  1306   0.0    1
AF151101|AF151101.1 Polysphondylium pallidum cortexillin I gene, complete cds.   141   5e-51  6
L46371|L46371.1 Dictyostelium discoideum cortexillin II mRNA, complete cds.    42   5e-04  2
AC115581|AC115581.3 Dictyostelium discoideum chromosome 2 map complement(3527391-3470188) strain AX4, complete sequence.    42   9e-04  5
AC146883|AC146883.2 Callithrix jacchus clone CH259-225J7, WORKING DRAFT SEQUENCE, 4 ordered pieces.    44   0.054  4
AL162405|AL162405.3 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP5-869B24.    48   0.25   1
BX537168|BX537168.4 Zebrafish DNA sequence from clone DKEY-105O6 in linkage group 4.    48   0.25   1
AL354956|AL354956.13 Human DNA sequence from clone RP5-1113E3 on chromosome  1p36.21-36.33.    48   0.25   1
AX430298|AX430298.1 Sequence 80 from Patent WO0240715.             36   0.39   2
AR448144|AR448144.1 Sequence 822 from patent US 6673549.           36   0.39   2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Y0T4) RecName: Full=Cortexillin-1; AltName: Full=Cortexillin ...   297   4e-79
L08068_1(L08068|pid:none) Physarum polycephalum actin-binding pr...    67   1e-09
FADOAA(S00103;A29006) alpha-actinin - slime mold (Dictyostelium ...    54   5e-06
A47297(A47297;A55441) myosin heavy chain form B, nonmuscle - Afr...    50   8e-05
BC108564_1(BC108564|pid:none) Xenopus laevis hypothetical protei...    50   8e-05
EF512630_1(EF512630|pid:none) Bos taurus alpha-actinin 1 (ACTN1)...    50   8e-05
AK304554_1(AK304554|pid:none) Homo sapiens cDNA FLJ58087 complet...    50   8e-05
(Q9QXQ0) RecName: Full=Alpha-actinin-4; AltName: Full=Non-muscle...    50   8e-05
AK150044_1(AK150044|pid:none) Mus musculus bone marrow macrophag...    50   8e-05
D89980_1(D89980|pid:none) Homo sapiens mRNA for alpha actinin 4,...    50   8e-05

 psg: 0.66  gvh: 0.55  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.33  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for CHB186 is cyt

>CHB186F.Seq
CACTGTTGGCCTACTGGTTTTTATTTATACACAATACATATAAAAGATGGCAGGTAAAGA
TTGGGAAATAGTACAAGAAAAAGCATTTACTGCATGGGTAAATTCAGTTTTAGATAAAAG
AGGTGAAAAGATTTCAGATGTCGGTAAAGACTTGAGTGATGGTGTCAAATTAATCTTCTT
TTTGGAACTCATCAGTNNNNNNNNNN

>CHB186Z.Seq
NNNNNNNNNNTGGTACCACTGATGAACGTTCACTCGTTCTCTACACATCTTTATTCTTCC
ATGCCTATAGAGCCAAGGAAGAGAAAGCTCGTTTAGAGAGTAGCAAAAATGAAATGGCAA
ATCGTTTAGCCGGTTTAGAGAATTCATTGGAATCTGAAAAGGTCTCACGTGAACAATTAA
TCAAACAAAAGGATCAACTCAATAGTTTATTGGCATCATTAGAGTCCGAAGGTGCTGAAC
GTGAAAAGAGACTTCGTGAATTGGAAGCCAAACTCGATGAAACCTTAAAGAACTTGGAAT
TAGAGAAATTGGCTCGTATGGAACTCGAAGCAAGATTAGCCAAGACCGAAAAGGATAGAG
CTATCTTGGAATTGAAATTAGCTGAAGCCATCGATGAAAAATCAAAACTCGAACAACAAA
TCGAAGCCACCAGAATCAGAGGTGCTGCTGAAGCTCAAGGTCTTGGTCTTTTAAGAAAGA
ACCTCGACACTCACGTTCACGATTTACTCAAATGGCAAAAGTTAACAATGGAAAACTCTT
CCTCATCCTCTATCGATGACCAAATCATCGTCGAAGTCTCTGGTCTTCCATTTGGTGAAC
AAGTTAAACATTTAGCCACAAAATTAGAAGCAGAAAATTTAGCAATTATGAAACTTTTAA
ATCAAAAAGAAGANATTTAAAAGCTCAAAAATTAAAATCATCAAAATCAAAAAAATAAAT
TAAAAAAA

>CHB186P.Seq
CACTGTTGGCCTACTGGTTTTTATTTATACACAATACATATAAAAGATGGCAGGTAAAGA
TTGGGAAATAGTACAAGAAAAAGCATTTACTGCATGGGTAAATTCAGTTTTAGATAAAAG
AGGTGAAAAGATTTCAGATGTCGGTAAAGACTTGAGTGATGGTGTCAAATTAATCTTCTT
TTTGGAACTCATCAGT----------TGGTACCACTGATGAACGTTCACTCGTTCTCTAC
ACATCTTTATTCTTCCATGCCTATAGAGCCAAGGAAGAGAAAGCTCGTTTAGAGAGTAGC
AAAAATGAAATGGCAAATCGTTTAGCCGGTTTAGAGAATTCATTGGAATCTGAAAAGGTC
TCACGTGAACAATTAATCAAACAAAAGGATCAACTCAATAGTTTATTGGCATCATTAGAG
TCCGAAGGTGCTGAACGTGAAAAGAGACTTCGTGAATTGGAAGCCAAACTCGATGAAACC
TTAAAGAACTTGGAATTAGAGAAATTGGCTCGTATGGAACTCGAAGCAAGATTAGCCAAG
ACCGAAAAGGATAGAGCTATCTTGGAATTGAAATTAGCTGAAGCCATCGATGAAAAATCA
AAACTCGAACAACAAATCGAAGCCACCAGAATCAGAGGTGCTGCTGAAGCTCAAGGTCTT
GGTCTTTTAAGAAAGAACCTCGACACTCACGTTCACGATTTACTCAAATGGCAAAAGTTA
ACAATGGAAAACTCTTCCTCATCCTCTATCGATGACCAAATCATCGTCGAAGTCTCTGGT
CTTCCATTTGGTGAACAAGTTAAACATTTAGCCACAAAATTAGAAGCAGAAAATTTAGCA
ATTATGAAACTTTTAAATCAAAAAGAAGANATTTAAAAGCTCAAAAATTAAAATCATCAA
AATCAAAAAAATAAATTAAAAAAA