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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB169
Library CH
(Link to library)
Clone ID CHB169
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12930-1|Contig-U16267-1
Original site URL
Representative seq. ID CHB169P
(Link to Original site)
Representative DNA sequence 
>CHB169 (CHB169Q) /CSM/CH/CHB1-C/CHB169Q.Seq.d/
GGCCTACTGGAAAAATAATATAATATAATATAATTAAATATATAAAATGAGCAATGAAGT
TATTAATGTATTAATTACTGGTGCAGCAGGTCAAATTGCATATTCATTAATTTTCAATGT
ATGTAAAGGTGATATGTTTGGTTTAGATCAACGTATTAAATTACATCTTTTAGATATTCC
ACAAATGGTAGACTCATTGAAAGGTATTGTAATGGAGATTCAAGATGGTGCATATCCATT
GATCGCTGATACAGTTATAACTGCCGATGTTAAAGAAGCATTCACTGGTGTACATTATGC
AATTTTAGTTGGTGCTATGCCACGTAGAGAAGGTATGGAAAGAGCTGATCTTTTGAAAGC
AAACGCAGCAATCTTCAAAGTTCAAGGTAAAGCACTCGCCGAACATGCAAATAAGAATGT
TAAAGTATTGGTAGTTGGTAXXXXXXXXXXAAAAAANNNNTTTTNNAAAAAAATNNNNNC
NTCNAANTTTTAAAGAAAAAANTNAAAAAAAAAAAAAAAAAAAAAAAAAATTTATTANCN
GGATNAAAAAAAAAAATAAAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
aywknnii*yn*IYKMSNEVINVLITGAAGQIAYSLIFNVCKGDMFGLDQRIKLHLLDIP
QMVDSLKGIVMEIQDGAYPLIADTVITADVKEAFTGVHYAILVGAMPRREGMERADLLKA
NAAIFKVQGKALAEHANKNVKVLVVG---

---KKXXXXKNXXXXXLKKKXKKKKKKKKNLLXGXKKKIKKK
Translated Amino Acid sequence (All Frames) 
Frame A:
gllek*ynii*lni*neq*sy*cinywcsrsncifinfqcm*r*yvwfrsty*itsfrys
tngrlierycngdsrwcisidr*ysyncrc*rsihwctlcnfswcyat*rrygks*sfes
krsnlqssr*strrtck*ec*sigsw---

---KKXXXXKNXXXXXLKKKXKKKKKKKKNLLXGXKKKIKKK

Frame B:
aywknnii*yn*IYKMSNEVINVLITGAAGQIAYSLIFNVCKGDMFGLDQRIKLHLLDIP
QMVDSLKGIVMEIQDGAYPLIADTVITADVKEAFTGVHYAILVGAMPRREGMERADLLKA
NAAIFKVQGKALAEHANKNVKVLVVG---

---kxxfxkkxxxsxf*rkxxkkkkkkkkiyxxdxkkk*kkk

Frame C:
ptgkii*yniikyik*amkllmy*llvqqvklhih*fsmyvkviclv*invlnyif*ifh
kw*th*kvl*wrfkmvhih*sliql*lpmlkkhslvyimqf*lvlchvekvwkelif*kq
tqqsskfkvkhspnmqirmlkyw*lv---

---kxxfxkkxxxxxfkekxxkkkkkkkkfixxxkkknkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN856 (VFN856Q) /CSM/VF/VFN8-C/VFN856Q.Seq.d/                        777   0.0
VFL218 (VFL218Q) /CSM/VF/VFL2-A/VFL218Q.Seq.d/                        777   0.0
VFK307 (VFK307Q) /CSM/VF/VFK3-A/VFK307Q.Seq.d/                        777   0.0
VFK265 (VFK265Q) /CSM/VF/VFK2-C/VFK265Q.Seq.d/                        777   0.0
VFE489 (VFE489Q) /CSM/VF/VFE4-D/VFE489Q.Seq.d/                        777   0.0
VFD713 (VFD713Q) /CSM/VF/VFD7-A/VFD713Q.Seq.d/                        777   0.0
VFC868 (VFC868Q) /CSM/VF/VFC8-C/VFC868Q.Seq.d/                        777   0.0
SFK157 (SFK157Q) /CSM/SF/SFK1-C/SFK157Q.Seq.d/                        777   0.0
SFJ624 (SFJ624Q) /CSM/SF/SFJ6-A/SFJ624Q.Seq.d/                        777   0.0
SFD844 (SFD844Q) /CSM/SF/SFD8-B/SFD844Q.Seq.d/                        777   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CK149703|CK149703.1 CsmgEST00186 Culicoides sonorensis female serum-fed midgut cDNAdehydrogenase activity, mRNA sequence.    38   1e-04  3
CB073641|CB073641.1 taa19b04.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.003  2
CF654456|CF654456.1 tac81f01.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CF777357|CF777357.1 tad09f04.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CF601602|CF601602.1 tac31e11.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CN568806|CN568806.1 tag51e05.y1 Hydra EST -Kiel 2 Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CF656636|CF656636.1 tac62d07.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CF656238|CF656238.1 tac51h05.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CF657720|CF657720.1 tac70h02.y1 Hydra EST -IV Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
CN556974|CN556974.1 tae39c05.y1 Hydra EST Darmstadt I Hydra magnipapillata cDNA 5' similar to SW:MDHC_MOUSE P14152 MALATE DEHYDROGENASE, CYTOPLASMIC ;, mRNA sequence.    44   0.004  2
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54GE6) RecName: Full=Probable malate dehydrogenase 1;         ...   140   2e-32
BT043497_1(BT043497|pid:none) Salmo salar clone HM4_0203 malate ...   137   1e-31
EU863454_1(EU863454|pid:none) Lottia austrodigitalis cytosolic m...   137   1e-31
(Q2YAQ4) RecName: Full=Malate dehydrogenase;          EC=1.1.1.3...   137   2e-31
AP009384_4186(AP009384|pid:none) Azorhizobium caulinodans ORS 57...   137   2e-31
AF390559_1(AF390559|pid:none) Sphyraena idiastes cytosolic malat...   137   2e-31
BT045320_1(BT045320|pid:none) Salmo salar clone ssal-rgf-518-137...   137   2e-31
EU569765_1(EU569765|pid:none) Ctenopharyngodon idella cytosolic ...   136   3e-31
EU863453_1(EU863453|pid:none) Lottia pelta cytosolic malate dehy...   136   4e-31
CP000781_1659(CP000781|pid:none) Xanthobacter autotrophicus Py2,...   135   6e-31
protein update 2009. 3.29
PSORT 

 psg: 0.91  gvh: 0.44  alm: 0.38  top: 0.40  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.64  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for CHB169 is nuc
5' end seq. ID CHB169F
5' end seq. 
>CHB169F.Seq
GGCCTACTGGAAAAATAATATAATATAATATAATTAAATATATAAAATGAGCAATGAAGT
TATTAATGTATTAATTACTGGTGCAGCAGGTCAAATTGCATATTCATTAATTTTCAATGT
ATGTAAAGGTGATATGTTTGGTTTAGATCAACGTATTAAATTACATCTTTTAGATATTCC
ACAAATGGTAGACTCATTGAAAGGTATTGTAATGGAGATTCAAGATGGTGCATATCCATT
GATCGCTGATACAGTTATAACTGCCGATGTTAAAGAAGCATTCACTGGTGTACATTATGC
AATTTTAGTTGGTGCTATGCCACGTAGAGAAGGTATGGAAAGAGCTGATCTTTTGAAAGC
AAACGCAGCAATCTTCAAAGTTCAAGGTAAAGCACTCGCCGAACATGCAAATAAGAATGT
TAAAGTATTGGTAGTTGGTANNNNNNNNNN
Length of 5' end seq. 450
3' end seq. ID CHB169Z
3' end seq. 
>CHB169Z.Seq
NNNNNNNNNNAAAAAANNNNTTTTNNAAAAAAATNNNNNCNTCNAANTTTTAAAGAAAAA
ANTNAAAAAAAAAAAAAAAAAAAAAAAAAATTTATTANCNGGATNAAAAAAAAAAATAAA
AAAAAAAAA
Length of 3' end seq. 129
Connected seq. ID CHB169P
Connected seq. 
>CHB169P.Seq
GGCCTACTGGAAAAATAATATAATATAATATAATTAAATATATAAAATGAGCAATGAAGT
TATTAATGTATTAATTACTGGTGCAGCAGGTCAAATTGCATATTCATTAATTTTCAATGT
ATGTAAAGGTGATATGTTTGGTTTAGATCAACGTATTAAATTACATCTTTTAGATATTCC
ACAAATGGTAGACTCATTGAAAGGTATTGTAATGGAGATTCAAGATGGTGCATATCCATT
GATCGCTGATACAGTTATAACTGCCGATGTTAAAGAAGCATTCACTGGTGTACATTATGC
AATTTTAGTTGGTGCTATGCCACGTAGAGAAGGTATGGAAAGAGCTGATCTTTTGAAAGC
AAACGCAGCAATCTTCAAAGTTCAAGGTAAAGCACTCGCCGAACATGCAAATAAGAATGT
TAAAGTATTGGTAGTTGGTA----------AAAAAANNNNTTTTNNAAAAAAATNNNNNC
NTCNAANTTTTAAAGAAAAAANTNAAAAAAAAAAAAAAAAAAAAAAAAAATTTATTANCN
GGATNAAAAAAAAAAATAAAAAAAAAAAA
Length of connected seq. 559
Full length Seq ID -
Full length Seq. -
Length of full length seq. -