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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB159
Library CH
(Link to library)
Clone ID CHB159
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16419-1
Original site URL
Representative seq. ID CHB159P
(Link to Original site)
Representative DNA sequence 
>CHB159 (CHB159Q) /CSM/CH/CHB1-C/CHB159Q.Seq.d/
ACTGTTGGCCTACTGGATTATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAAA
CAATANCTAACTAAGTTAAAAATAAAAAAATAATAAAGGNTGTTGTTTNTAAAAAATATT
GGAGTATTTTTTATGATATTCCTTGTNTCAAAATCCTATGCGACGGATTGTAATAAAATT
NCTAATGAAGAANAATGTCATAAATCTTCGGAATGTNTTGTTNTAAATTATNCNCCATGT
TGGGGNGAACAAAAATGGGCTTGCTNTAAAGGTNCATTTGATACTTGTNCATATGAAAAT
AGTTGTTATANAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAANGCTTCAAT
CTTGATGGATTTATTAAAATTACAACTCCAACCGAGTXXXXXXXXXXTGTGTAAAGGTNC
ACCCNTGATAGATGTTCATTAANATGCCCNCATGNCCATGAATGTAAAGTTGATCCCAAT
GGTAAAGAATGTTGGGTCAGATCCCATAGNGCCACCACCANCAGGAGTNTGTTCATTAAG
GATGCCCACCAAAACATGAATGTAGGTTTGATGATCATGGTAAAAAATGTTGTGTAAAGA
TTCATTGTGATGNAGTTTGTGATNTAGATTGTGGTANAGGTTTTGAATGTAAAATTAGAC
ATGATGGTTCANAATGTTGTGTCCGTTCAGAAAGACCACACCCACCACAACATGAANAAT
GTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTT
GCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAAT
GTAGAGTCANCCACTTTGGTGAAGAATGTTGTGTTAAAGTTCACCNCGATNAATGTTCAT
TGAGATGNCCNCCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTG
TTGCCCATAGACCACCACCNTAATGCTCATTAAGATGTCCNCCAAAACATGAATGTAGAA
TCNATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGNAATGATTGTTTAACTTGTGAAG
ACTTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAAAACTG
sequence update 2002.10.25
Translated Amino Acid sequence 
tvglldysilflinsn*kkkqxltklkikk**RXLFXKNIGVFFMIFLVSKSYATDCNKI
XNEEXCHKSSECXVXNYXPCWGEQKWACXKGXFDTCXYENSCYXNSSNNQVVEVSNKXFN
LDGFIKITTPTE---

---CKGXPXIDVH*xaxmxmnvklipmvknvgsdpixpppxgvcslrmptkt*m*v**sw
*kmlckdsl*xsl*xrlwxrf*m*n*t*wfxmlcpfrkttpttt*xm**emptrp*m*s*
stwkrmlrccp*tttkmlfkmstkt*m*sxplw*rmlc*ssprxmfiemxxrp*m*s*st
wkrmlrccp*tttxmlikmsxkt*m*nxslw*rmlc*k*x*lfnl*rlkl*kkrftlche
n
Translated Amino Acid sequence (All Frames) 
Frame A:
tvglldysilflinsn*kkkqxltklkikk**RXLFXKNIGVFFMIFLVSKSYATDCNKI
XNEEXCHKSSECXVXNYXPCWGEQKWACXKGXFDTCXYENSCYXNSSNNQVVEVSNKXFN
LDGFIKITTPTE---

---cvkvhp**mfixmpxxp*m*s*sqw*rmlgqip*xhhxqexvh*gcppkhecrfddh
gkkccvkihcdxvcdxdcgxgfeckirhdgsxccvrserphppqhexcnkrcppgheckv
dqhgkeccvvahrpppkcslrcpprhecrvxhfgeeccvkvhxdxcslrxppgheckvdq
hgkeccvvahrppp*cslrcppkhecrixhfgeeccvksxndcltcedlncerkglhcam
kt

Frame B:
llaywiiqfyf*siqikkknnx*ls*k*knnkgccx*kileyfl*yslxqnpmrrivikx
lmkxnvinlrnvlx*ixxhvgxnknglaxkvhlilvhmkivvixihpitkllkyqixasi
lmdllklqlqps---

---v*rxtxdrcslxcphxheckvdpngkecwvrshxattxrsxfikdahqnmnvglmim
vknvv*rfivmxfvx*ivvxvlnvkldmmvxnvvsvqkdhthhnmxnvirdahqamnvkl
inmeknvallpidhhqnal*dvhqdmnvesxtlvknvvlkftxxnvh*dxxqamnvklin
meknvallpidhhxnah*dvxqnmnvesitlvknavlkvxmiv*lvkt*tvkekvyivp*
kl

Frame C:
cwptglfnfifnqfklkkktixn*vknkkiikxvvxkkywsifydipcxkilcdgl**nx
**rxms*ifgmxcxklxxmlgxtkmgll*rxi*ylxi*k*llxkfiq*psc*sik*xlqs
*wiy*nynsnr---

---CKGXPXIDVH*xaxmxmnvklipmvknvgsdpixpppxgvcslrmptkt*m*v**sw
*kmlckdsl*xsl*xrlwxrf*m*n*t*wfxmlcpfrkttpttt*xm**emptrp*m*s*
stwkrmlrccp*tttkmlfkmstkt*m*sxplw*rmlc*ssprxmfiemxxrp*m*s*st
wkrmlrccp*tttxmlikmsxkt*m*nxslw*rmlc*k*x*lfnl*rlkl*kkrftlche
n
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB159 (CHB159Q) /CSM/CH/CHB1-C/CHB159Q.Seq.d/                       1772   0.0
AHE565 (AHE565Q) /CSM/AH/AHE5-C/AHE565Q.Seq.d/                       1205   0.0
CHH443 (CHH443Q) /CSM/CH/CHH4-B/CHH443Q.Seq.d/                       1199   0.0
AHO313 (AHO313Q) /CSM/AH/AHO3-A/AHO313Q.Seq.d/                       1195   0.0
AHJ552 (AHJ552Q) /CSM/AH/AHJ5-C/AHJ552Q.Seq.d/                       1193   0.0
CHM493 (CHM493Q) /CSM/CH/CHM4-D/CHM493Q.Seq.d/                       1191   0.0
CHH465 (CHH465Q) /CSM/CH/CHH4-C/CHH465Q.Seq.d/                       1191   0.0
AHQ620 (AHQ620Q) /CSM/AH/AHQ6-A/AHQ620Q.Seq.d/                       1191   0.0
AHO207 (AHO207Q) /CSM/AH/AHO2-A/AHO207Q.Seq.d/                       1191   0.0
CHI363 (CHI363Q) /CSM/CH/CHI3-C/CHI363Q.Seq.d/                       1189   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.  1045   0.0    9
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.  1045   0.0    10
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.    58   5e-10  4
X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.    58   1e-09  4
AC145220|AC145220.10 Medicago truncatula clone mth2-24j17, WORKING DRAFT SEQUENCE, 3 unordered pieces.    42   0.006  2
AC141955|AC141955.2 Rattus norvegicus clone CH230-418P10, WORKING DRAFT SEQUENCE, 29 unordered pieces.    46   0.22   4
AC127160|AC127160.3 Rattus norvegicus clone CH230-460D14, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    46   1.2    1
AZ682878|AZ682878.1 ENTGO10TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38   1.3    2
CN585252|CN585252.1 USDA-FP_128322 Acyrthosiphon pisum, Pea Aphid Acyrthosiphon pisum cDNA clone WHAP070_C11, mRNA sequence.    38   2.4    2
AY156074|AY156074.1 Entamoeba histolytica cysteine protease 16 (CP16) gene, complete cds.    38   2.7    2
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...   403   e-122
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   155   2e-36
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    99   2e-19
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    61   8e-08
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        54   7e-06
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    50   1e-04
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    50   1e-04
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    50   1e-04
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    48   5e-04
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    47   0.001
protein update 2009. 3.29
PSORT 

 psg: 1.02  gvh: 0.68  alm: 0.53  top: 0.27  tms: 0.00  mit: 0.40  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for CHB159 is exc
5' end seq. ID CHB159F
5' end seq. 
>CHB159F.Seq
ACTGTTGGCCTACTGGATTATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAAA
CAATANCTAACTAAGTTAAAAATAAAAAAATAATAAAGGNTGTTGTTTNTAAAAAATATT
GGAGTATTTTTTATGATATTCCTTGTNTCAAAATCCTATGCGACGGATTGTAATAAAATT
NCTAATGAAGAANAATGTCATAAATCTTCGGAATGTNTTGTTNTAAATTATNCNCCATGT
TGGGGNGAACAAAAATGGGCTTGCTNTAAAGGTNCATTTGATACTTGTNCATATGAAAAT
AGTTGTTATANAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAANGCTTCAAT
CTTGATGGATTTATTAAAATTACAACTCCAACCGAGTNNNNNNNNNN
Length of 5' end seq. 407
3' end seq. ID CHB159Z
3' end seq. 
>CHB159Z.Seq
NNNNNNNNNNTGTGTAAAGGTNCACCCNTGATAGATGTTCATTAANATGCCCNCATGNCC
ATGAATGTAAAGTTGATCCCAATGGTAAAGAATGTTGGGTCAGATCCCATAGNGCCACCA
CCANCAGGAGTNTGTTCATTAAGGATGCCCACCAAAACATGAATGTAGGTTTGATGATCA
TGGTAAAAAATGTTGTGTAAAGATTCATTGTGATGNAGTTTGTGATNTAGATTGTGGTAN
AGGTTTTGAATGTAAAATTAGACATGATGGTTCANAATGTTGTGTCCGTTCAGAAAGACC
ACACCCACCACAACATGAANAATGTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGT
TGATCAACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTT
AAGATGTCCACCAAGACATGAATGTAGAGTCANCCACTTTGGTGAAGAATGTTGTGTTAA
AGTTCACCNCGATNAATGTTCATTGAGATGNCCNCCAGGCCATGAATGTAAAGTTGATCA
ACATGGAAAAGAATGTTGCGTTGTTGCCCATAGACCACCACCNTAATGCTCATTAAGATG
TCCNCCAAAACATGAATGTAGAATCNATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAG
NAATGATTGTTTAACTTGTGAAGACTTAAACTGTGAAAGAAAAGGTTTACATTGTGCCAT
GAAAACTG
Length of 3' end seq. 728
Connected seq. ID CHB159P
Connected seq. 
>CHB159P.Seq
ACTGTTGGCCTACTGGATTATTCAATTTTATTTTTAATCAATTCAAATTAAAAAAAAAAA
CAATANCTAACTAAGTTAAAAATAAAAAAATAATAAAGGNTGTTGTTTNTAAAAAATATT
GGAGTATTTTTTATGATATTCCTTGTNTCAAAATCCTATGCGACGGATTGTAATAAAATT
NCTAATGAAGAANAATGTCATAAATCTTCGGAATGTNTTGTTNTAAATTATNCNCCATGT
TGGGGNGAACAAAAATGGGCTTGCTNTAAAGGTNCATTTGATACTTGTNCATATGAAAAT
AGTTGTTATANAAATTCATCCAATAACCAAGTTGTTGAAGTATCAAATAAANGCTTCAAT
CTTGATGGATTTATTAAAATTACAACTCCAACCGAGT----------TGTGTAAAGGTNC
ACCCNTGATAGATGTTCATTAANATGCCCNCATGNCCATGAATGTAAAGTTGATCCCAAT
GGTAAAGAATGTTGGGTCAGATCCCATAGNGCCACCACCANCAGGAGTNTGTTCATTAAG
GATGCCCACCAAAACATGAATGTAGGTTTGATGATCATGGTAAAAAATGTTGTGTAAAGA
TTCATTGTGATGNAGTTTGTGATNTAGATTGTGGTANAGGTTTTGAATGTAAAATTAGAC
ATGATGGTTCANAATGTTGTGTCCGTTCAGAAAGACCACACCCACCACAACATGAANAAT
GTAATAAGAGATGCCCACCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTT
GCGTTGTTGCCCATAGACCACCACCAAAATGCTCTTTAAGATGTCCACCAAGACATGAAT
GTAGAGTCANCCACTTTGGTGAAGAATGTTGTGTTAAAGTTCACCNCGATNAATGTTCAT
TGAGATGNCCNCCAGGCCATGAATGTAAAGTTGATCAACATGGAAAAGAATGTTGCGTTG
TTGCCCATAGACCACCACCNTAATGCTCATTAAGATGTCCNCCAAAACATGAATGTAGAA
TCNATCACTTTGGTGAAGAATGCTGTGTTAAAAGTAGNAATGATTGTTTAACTTGTGAAG
ACTTAAACTGTGAAAGAAAAGGTTTACATTGTGCCATGAAAACTG
Length of connected seq. 1115
Full length Seq ID -
Full length Seq. -
Length of full length seq. -