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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB142
Library CH
(Link to library)
Clone ID CHB142
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16450-1
Original site URL
Representative seq. ID CHB142P
(Link to Original site)
Representative DNA sequence 
>CHB142 (CHB142Q) /CSM/CH/CHB1-B/CHB142Q.Seq.d/
AAAAATCTTTTTTTTTATTGTTTTAAATATTTTTTTTAAAATGAAAAAAATAATATTATT
TTTAACAGTACTACTTGTATTATTATTTGGTAAAATTGAATCACATTCATTTATGAAATG
TGTGGATTGGGATTTTGGTTCAAATTCATGTGCAGCATACCCAAGAAACTATAGATTTTA
TGAACAACAAGGCGATGGTTTCTTATTCCAAAGAGGTAAATTCACAAAACCATACCCAAC
TGAATTGGGTCAATCATGTGGCCCAGCACAAAGAAGAAGTTCTTCAATTACCAATCTTTA
TAGTTCAAAACATCCAATGGGAAATTTCCAAGCTGGTCAAAAGATTTGCCCACAATGGCC
AGCAAGAAACCATGCGACTGCATTAAGAGCTGGTACCGTTAGTTTCTACCTTTCTCCAAA
AATGACATCAGATGCTCCCGXXXXXXXXXXTCGAGAAGAATACCCACAAAGACCATCTCG
TGAAGAACTTCAAAGAGAAGAAAGACAAAGAGATCAAGAAGAACAAGCCATAAGAAGAAA
GAGACAAAATGATGAGTATCTTGAATTGCAAAGAGAAAAAGAAAGAGAACAAATGCAACG
TGATATAGAGAGAAAAGCTGCAGCAGAAGAGTCCAGAAAACATAAATCAACCATTTTCCA
ATATATTATGAGAGATGGTAAATTGGAAAGAGAATTCTCAAGTGCAAGTTGGGGTCCATA
TTCAAGAAGGAGGTTGAATATTATGAAACTAGACCAATACTTGGAAATGTTTTATTCTTT
AAATGTGCGACTGGATCCAATTGTTTATCAAGTAAAGGCAAAAGAACCCTCAACATCAAC
ATTTTAGGTTTTAAAGAAATCATTTCAAATACTCAAGTTAACCTCTTAACCAAAGGTGAA
AAAATTCCACTCTCAATTTCATTAGAACCATACATTGATTCATCATTTATTGATGATGGT
GATTCATACAATACAATTAGCGTTCCACTTCAAAGATTCTATAGAAATGAATTCTATGCT
ATTACTCTATCTGGTTATGANATTCAAATAGNATATTATTAGAGTTCAATCAATTTCACT
TTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KIFFFIVLNIFFKMKKIILFLTVLLVLLFGKIESHSFMKCVDWDFGSNSCAAYPRNYRFY
EQQGDGFLFQRGKFTKPYPTELGQSCGPAQRRSSSITNLYSSKHPMGNFQAGQKICPQWP
ARNHATALRAGTVSFYLSPKMTSDAP---

---REEYPQRPSREELQREERQRDQEEQAIRRKRQNDEYLELQREKEREQMQRDIERKAA
AEESRKHKSTIFQYIMRDGKLEREFSSASWGPYSRRRLNIMKLDQYLEMFYSLNVRLDPI
VYQVKAKEPSTSTF*vlkksfqilklts*pkvkkfhsqfh*nhtlihhllmmvihtiqla
fhfkdsiemnsmlllylvmxfk*xiirvqsisls
Translated Amino Acid sequence (All Frames) 
Frame A:
knlffycfkyff*neknniifnsttciiiw*n*itfiyemcglgfwfkfmcsipkkl*il
*ttrrwflipkr*ihktipn*igsimwpstkkkffnyqsl*fktsngkfpswskdlptma
skkpcdcikswyr*flpfskndircs---

---srriptktis*rtskrrktkrsrrtshkkketk**vs*iakrkrkrtnat*yreksc
srrvqkt*inhfpiyyerw*igkrilkcklgsifkkeveyyetrpilgnvlffkcatgsn
clsskgkrtlninilgfkeiisntqvnlltkgekiplsislepyidssfiddgdsyntis
vplqrfyrnefyaitlsgyxiqixyy*ssinftfk

Frame B:
KIFFFIVLNIFFKMKKIILFLTVLLVLLFGKIESHSFMKCVDWDFGSNSCAAYPRNYRFY
EQQGDGFLFQRGKFTKPYPTELGQSCGPAQRRSSSITNLYSSKHPMGNFQAGQKICPQWP
ARNHATALRAGTVSFYLSPKMTSDAP---

---REEYPQRPSREELQREERQRDQEEQAIRRKRQNDEYLELQREKEREQMQRDIERKAA
AEESRKHKSTIFQYIMRDGKLEREFSSASWGPYSRRRLNIMKLDQYLEMFYSLNVRLDPI
VYQVKAKEPSTSTF*vlkksfqilklts*pkvkkfhsqfh*nhtlihhllmmvihtiqla
fhfkdsiemnsmlllylvmxfk*xiirvqsisls

Frame C:
ksffllf*ifflk*kk*yyf*qyylyyylvklnhihl*nvwigilvqihvqhtqetidfm
nnkamvsyskevnsqnhtqlnwvnhvaqhkeevlqlpifivqniqweisklvkrfahngq
qetmrlh*elvplvstflqk*hqmlp---

---eknthkdhlvknfkekkdkeikknkp*eerdkmmsilnckekkkenkcnvi*reklq
qkspeninqpfsnil*emvnwkensqvqvgvhiqegg*il*n*tntwkcfil*mcdwiql
fik*rqknpqhqhfrf*rnhfkyss*plnqr*knstlnfirtih*fiiy**w*fiqyn*r
stskil*k*ilcyysiwl*xsnxillefnqfhfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB142 (CHB142Q) /CSM/CH/CHB1-B/CHB142Q.Seq.d/                       1063   0.0
CFK415 (CFK415Q) /CSM/CF/CFK4-A/CFK415Q.Seq.d/                       1031   0.0
SFB309 (SFB309Q) /CSM/SF/SFB3-A/SFB309Q.Seq.d/                       1027   0.0
SFB634 (SFB634Q) /CSM/SF/SFB6-B/SFB634Q.Seq.d/                       1025   0.0
CHD204 (CHD204Q) /CSM/CH/CHD2-A/CHD204Q.Seq.d/                       1017   0.0
SFJ341 (SFJ341Q) /CSM/SF/SFJ3-B/SFJ341Q.Seq.d/                       1015   0.0
CFI629 (CFI629Q) /CSM/CF/CFI6-B/CFI629Q.Seq.d/                       1015   0.0
CFI518 (CFI518Q) /CSM/CF/CFI5-A/CFI518Q.Seq.d/                       1015   0.0
CFH476 (CFH476Q) /CSM/CF/CFH4-D/CFH476Q.Seq.d/                       1015   0.0
CFF459 (CFF459Q) /CSM/CF/CFF4-C/CFF459Q.Seq.d/                       1015   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC131557|AC131557.4 Rattus norvegicus clone CH230-8O19, *** SEQUENCING IN PROGRESS ***.    34   0.005  10
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.    34   0.005  13
AE017337|AE017337.1 Mycoplasma mobile 163K section 1 of 3 of...    36   0.006  13
AE017263|AE017263.1 Mesoplasma florum L1 complete genome.          32   0.008  21
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.013  15
AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   0.013  12
AC141714|AC141714.1 Apis mellifera clone CH224-57G22, *** SEQUENCING IN PROGRESS ***, 54 unordered pieces.    34   0.035  7
AC148414|AC148414.2 Callithrix jacchus clone CH259-31N19, WORKING DRAFT SEQUENCE, 6 ordered pieces.    50   0.076  1
AC116940|AC116940.2 Pan troglodytes clone RP43-166L2, WORKING DRAFT SEQUENCE, 1 ordered piece.    50   0.076  1
AC116436|AC116436.2 Pan troglodytes clone RP43-55P4, WORKING DRAFT SEQUENCE, 4 ordered pieces.    50   0.076  1
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5ZIA2) RecName: Full=Ensconsin; AltName: Full=Microtubule-asso...    39   0.30
CP000500_224(CP000500|pid:none) Pichia stipitis CBS 6054 chromos...    38   0.51
CR382130_32(CR382130|pid:none) Yarrowia lipolytica strain CLIB12...    35   4.3
CP000438_1475(CP000438|pid:none) Pseudomonas aeruginosa UCBPP-PA...    35   5.7
FM209186_1499(FM209186|pid:none) Pseudomonas aeruginosa LESB58 c...    35   5.7
AE004091_3535(AE004091|pid:none) Pseudomonas aeruginosa PAO1, co...    35   5.7
CP000744_1599(CP000744|pid:none) Pseudomonas aeruginosa PA7, com...    35   5.7
(P38232) RecName: Full=Protein REG2;  &AY557867_1(AY557867|pid:n...    35   5.7
AC007213_9(AC007213|pid:none) Arabidopsis thaliana chromosome 2 ...    34   9.7
protein update 2009. 3.29
PSORT 

 psg: 0.94  gvh: 0.72  alm: 0.53  top: 0.40  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.17  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal

>> prediction for CHB142 is exc
5' end seq. ID CHB142F
5' end seq. 
>CHB142F.Seq
AAAAATCTTTTTTTTTATTGTTTTAAATATTTTTTTTAAAATGAAAAAAATAATATTATT
TTTAACAGTACTACTTGTATTATTATTTGGTAAAATTGAATCACATTCATTTATGAAATG
TGTGGATTGGGATTTTGGTTCAAATTCATGTGCAGCATACCCAAGAAACTATAGATTTTA
TGAACAACAAGGCGATGGTTTCTTATTCCAAAGAGGTAAATTCACAAAACCATACCCAAC
TGAATTGGGTCAATCATGTGGCCCAGCACAAAGAAGAAGTTCTTCAATTACCAATCTTTA
TAGTTCAAAACATCCAATGGGAAATTTCCAAGCTGGTCAAAAGATTTGCCCACAATGGCC
AGCAAGAAACCATGCGACTGCATTAAGAGCTGGTACCGTTAGTTTCTACCTTTCTCCAAA
AATGACATCAGATGCTCCCGNNNNNNNNNN
Length of 5' end seq. 450
3' end seq. ID CHB142Z
3' end seq. 
>CHB142Z.Seq
NNNNNNNNNNTCGAGAAGAATACCCACAAAGACCATCTCGTGAAGAACTTCAAAGAGAAG
AAAGACAAAGAGATCAAGAAGAACAAGCCATAAGAAGAAAGAGACAAAATGATGAGTATC
TTGAATTGCAAAGAGAAAAAGAAAGAGAACAAATGCAACGTGATATAGAGAGAAAAGCTG
CAGCAGAAGAGTCCAGAAAACATAAATCAACCATTTTCCAATATATTATGAGAGATGGTA
AATTGGAAAGAGAATTCTCAAGTGCAAGTTGGGGTCCATATTCAAGAAGGAGGTTGAATA
TTATGAAACTAGACCAATACTTGGAAATGTTTTATTCTTTAAATGTGCGACTGGATCCAA
TTGTTTATCAAGTAAAGGCAAAAGAACCCTCAACATCAACATTTTAGGTTTTAAAGAAAT
CATTTCAAATACTCAAGTTAACCTCTTAACCAAAGGTGAAAAAATTCCACTCTCAATTTC
ATTAGAACCATACATTGATTCATCATTTATTGATGATGGTGATTCATACAATACAATTAG
CGTTCCACTTCAAAGATTCTATAGAAATGAATTCTATGCTATTACTCTATCTGGTTATGA
NATTCAAATAGNATATTATTAGAGTTCAATCAATTTCACTTTCAAA
Length of 3' end seq. 646
Connected seq. ID CHB142P
Connected seq. 
>CHB142P.Seq
AAAAATCTTTTTTTTTATTGTTTTAAATATTTTTTTTAAAATGAAAAAAATAATATTATT
TTTAACAGTACTACTTGTATTATTATTTGGTAAAATTGAATCACATTCATTTATGAAATG
TGTGGATTGGGATTTTGGTTCAAATTCATGTGCAGCATACCCAAGAAACTATAGATTTTA
TGAACAACAAGGCGATGGTTTCTTATTCCAAAGAGGTAAATTCACAAAACCATACCCAAC
TGAATTGGGTCAATCATGTGGCCCAGCACAAAGAAGAAGTTCTTCAATTACCAATCTTTA
TAGTTCAAAACATCCAATGGGAAATTTCCAAGCTGGTCAAAAGATTTGCCCACAATGGCC
AGCAAGAAACCATGCGACTGCATTAAGAGCTGGTACCGTTAGTTTCTACCTTTCTCCAAA
AATGACATCAGATGCTCCCG----------TCGAGAAGAATACCCACAAAGACCATCTCG
TGAAGAACTTCAAAGAGAAGAAAGACAAAGAGATCAAGAAGAACAAGCCATAAGAAGAAA
GAGACAAAATGATGAGTATCTTGAATTGCAAAGAGAAAAAGAAAGAGAACAAATGCAACG
TGATATAGAGAGAAAAGCTGCAGCAGAAGAGTCCAGAAAACATAAATCAACCATTTTCCA
ATATATTATGAGAGATGGTAAATTGGAAAGAGAATTCTCAAGTGCAAGTTGGGGTCCATA
TTCAAGAAGGAGGTTGAATATTATGAAACTAGACCAATACTTGGAAATGTTTTATTCTTT
AAATGTGCGACTGGATCCAATTGTTTATCAAGTAAAGGCAAAAGAACCCTCAACATCAAC
ATTTTAGGTTTTAAAGAAATCATTTCAAATACTCAAGTTAACCTCTTAACCAAAGGTGAA
AAAATTCCACTCTCAATTTCATTAGAACCATACATTGATTCATCATTTATTGATGATGGT
GATTCATACAATACAATTAGCGTTCCACTTCAAAGATTCTATAGAAATGAATTCTATGCT
ATTACTCTATCTGGTTATGANATTCAAATAGNATATTATTAGAGTTCAATCAATTTCACT
TTCAAA
Length of connected seq. 1076
Full length Seq ID -
Full length Seq. -
Length of full length seq. -