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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHB132
Library CH
(Link to library)
Clone ID CHB132
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16176-1
Original site URL
Representative seq. ID CHB132P
(Link to Original site)
Representative DNA sequence 
>CHB132 (CHB132Q) /CSM/CH/CHB1-B/CHB132Q.Seq.d/
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTANACCAATCATTGAATNTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGTXXXXX
XXXXXCTGAAGTTATCNATTTACGGACCNTTCGGCCNATTGATGCTGAAACCNTTGNAAA
TTCNCTCAAAAAAACNAATNAATTAGTTACCGTTNAANAAGGTTGGGCNCNATCTGGGAT
TGGGGCTGAAATCNCTGCNTTAATGATGGNACANGCTTTTGATTATTTANANGCCCCNAT
TGAAANAATTTGNGGNGCTGATGNTCCNANGCCANANGCTTCNAACTTGGAAAANGC
sequence update 2002.10.25
Translated Amino Acid sequence 
TILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELARDEKVFI
MGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGTXPIIEXMTFNF
AMQAIDHIINSSAKTHYM---

---EVIXLRTXRPIDAETXXNSLKKTNXLVTVXXGWAXSGIGAEIXALMMXXAFDYLXAP
IEXIXGADXPXPXASNLEX
Translated Amino Acid sequence (All Frames) 
Frame A:
TILTYHHSKMLSSILKKIQPSLLVNSRIITRTYATKEVTVRDAINSALDEELARDEKVFI
MGEEVAQYNGAYKITKGLFDKYGGDRIIDTPITEAGFAGIGVGAAMAGTXPIIEXMTFNF
AMQAIDHIINSSAKTHYM---

---lklsiygpfgxlmlkplxixskkxxn*lpxxkvgxxlglglkslx**wxxlliixxp
xlkxfxxlmxxxxxlxtwkx

Frame B:
qf*liiiqkcclqf*kkfnhhy*ltlelslelmllkrlqfvmqlivhwmkn*qemkkfll
wvkk*lnimvhtrslkdysinmvviellilqlqklvllvlvlvllwqvlxqslnx*lstl
lckqlitssihllkliic---

---*syxftdxsax*c*nxxkfxqknxxisyrxxrlgxiwdwg*nxcxndgtxf*lfxxp
x*xnlxx*xsxaxxfxlgkx

Frame C:
nfnlssfknvvfnfkknstiiis*l*nyh*nlcy*rgyss*cn**cig*riskr*ksfyy
g*rsssi*wciqdh*riir*iww**ny*ysnyrswfcwywcwccygryxtnh*xydfqlc
yasn*shhqfic*nsly---

---EVIXLRTXRPIDAETXXNSLKKTNXLVTVXXGWAXSGIGAEIXALMMXXAFDYLXAP
IEXIXGADXPXPXASNLEX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHB132 (CHB132Q) /CSM/CH/CHB1-B/CHB132Q.Seq.d/                        969   0.0
VFO521 (VFO521Q) /CSM/VF/VFO5-A/VFO521Q.Seq.d/                        763   0.0
VFO365 (VFO365Q) /CSM/VF/VFO3-C/VFO365Q.Seq.d/                        763   0.0
VFO292 (VFO292Q) /CSM/VF/VFO2-D/VFO292Q.Seq.d/                        763   0.0
VFN687 (VFN687Q) /CSM/VF/VFN6-D/VFN687Q.Seq.d/                        763   0.0
VFK807 (VFK807Q) /CSM/VF/VFK8-A/VFK807Q.Seq.d/                        763   0.0
VFI440 (VFI440Q) /CSM/VF/VFI4-B/VFI440Q.Seq.d/                        763   0.0
VFF768 (VFF768Q) /CSM/VF/VFF7-C/VFF768Q.Seq.d/                        763   0.0
SFD157 (SFD157Q) /CSM/SF/SFD1-C/SFD157Q.Seq.d/                        763   0.0
SFC894 (SFC894Q) /CSM/SF/SFC8-D/SFC894Q.Seq.d/                        763   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC114263|AC114263.2 Dictyostelium discoideum chromosome 2 map 215673-367476 strain AX4, complete sequence.   595   0.0    5
BQ860256|BQ860256.1 QGC15E06.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC15E06, mRNA sequence.    56   4e-19  4
BQ862298|BQ862298.1 QGC20L13.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC20L13, mRNA sequence.    56   4e-19  4
BQ862971|BQ862971.1 QGC22J22.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC22J22, mRNA sequence.    56   4e-19  4
BQ855000|BQ855000.1 QGB24M14.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGB24M14, mRNA sequence.    56   4e-19  4
CB086276|CB086276.1 hj82c12.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj82c12, mRNA sequence.    52   5e-17  3
CN520861|CN520861.1 GQ0107.B3_B15 GQ010 Populus balsamifera subsp. trichocarpa x Populus deltoides cDNA clone GQ0107_B15 5', mRNA sequence.    58   4e-16  2
CN519032|CN519032.1 GQ0102.B3_G15 GQ010 Populus balsamifera subsp. trichocarpa x Populus deltoides cDNA clone GQ0102_G15 5', mRNA sequence.    58   4e-16  2
BU881003|BU881003.1 UM57TE08 Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA 5 prime, mRNA sequence.    58   4e-16  2
BI120224|BI120224.1 F012P16Y Populus flower cDNA library Populus balsamifera subsp. trichocarpa cDNA, mRNA sequence.    58   4e-16  2
dna update 2004. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q86HX0) RecName: Full=Pyruvate dehydrogenase E1 component subun...   245   7e-64
EU962529_1(EU962529|pid:none) Zea mays clone 243474 pyruvate deh...   169   6e-41
AF069910_1(AF069910|pid:none) Zea mays pyruvate dehydrogenase E1...   169   6e-41
(Q38799) RecName: Full=Pyruvate dehydrogenase E1 component subun...   165   9e-40
U09137_1(U09137|pid:none) Arabidopsis thaliana pyruvate dehydrog...   165   9e-40
CR380957_290(CR380957|pid:none) Candida glabrata strain CBS138 c...   164   2e-39
AK104686_1(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   164   3e-39
AE014297_4486(AE014297|pid:none) Drosophila melanogaster chromos...   163   3e-39
BT001756_1(BT001756|pid:none) Drosophila melanogaster RH05604 fu...   163   3e-39
AK104686_2(AK104686|pid:none) Oryza sativa Japonica Group cDNA c...   163   5e-39
protein update 2009. 3.29
PSORT 

 psg: 0.71  gvh: 0.44  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: mitochondrial
	 28.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for CHB132 is mit
5' end seq. ID CHB132F
5' end seq. 
>CHB132F.Seq
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTANACCAATCATTGAATNTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGTNNNNN
NNNNN
Length of 5' end seq. 425
3' end seq. ID CHB132Z
3' end seq. 
>CHB132Z.Seq
NNNNNNNNNNCTGAAGTTATCNATTTACGGACCNTTCGGCCNATTGATGCTGAAACCNTT
GNAAATTCNCTCAAAAAAACNAATNAATTAGTTACCGTTNAANAAGGTTGGGCNCNATCT
GGGATTGGGGCTGAAATCNCTGCNTTAATGATGGNACANGCTTTTGATTATTTANANGCC
CCNATTGAAANAATTTGNGGNGCTGATGNTCCNANGCCANANGCTTCNAACTTGGAAAAN
GC
Length of 3' end seq. 242
Connected seq. ID CHB132P
Connected seq. 
>CHB132P.Seq
ACAATTTTAACTTATCATCATTCAAAAATGTTGTCTTCAATTTTAAAAAAAATTCAACCA
TCATTATTAGTTAACTCTAGAATTATCACTAGAACTTATGCTACTAAAGAGGTTACAGTT
CGTGATGCAATTAATAGTGCATTGGATGAAGAATTAGCAAGAGATGAAAAAGTTTTTATT
ATGGGTGAAGAAGTAGCTCAATATAATGGTGCATACAAGATCACTAAAGGATTATTCGAT
AAATATGGTGGTGATAGAATTATTGATACTCCAATTACAGAAGCTGGTTTTGCTGGTATT
GGTGTTGGTGCTGCTATGGCAGGTACTANACCAATCATTGAATNTATGACTTTCAACTTT
GCTATGCAAGCAATTGATCACATCATCAATTCATCTGCTAAAACTCATTATATGT-----
-----CTGAAGTTATCNATTTACGGACCNTTCGGCCNATTGATGCTGAAACCNTTGNAAA
TTCNCTCAAAAAAACNAATNAATTAGTTACCGTTNAANAAGGTTGGGCNCNATCTGGGAT
TGGGGCTGAAATCNCTGCNTTAATGATGGNACANGCTTTTGATTATTTANANGCCCCNAT
TGAAANAATTTGNGGNGCTGATGNTCCNANGCCANANGCTTCNAACTTGGAAAANGC
Length of connected seq. 647
Full length Seq ID -
Full length Seq. -
Length of full length seq. -