AFF321 [Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview]

AFF321
Library	AF (Link to library)
Clone ID	AFF321 (Link to dictyBase)
Atlas ID	-
NBRP ID	-
dictyBase ID	-
Link to Contig	Contig-U16507-1
Original site URL	http://dictycdb.biol.tsukuba.ac.jp/CSM/AF/AFF3-A/AFF321Q.Seq.d/
Representative seq. ID	AFF321P (Link to Original site)
Representative DNA sequence	>AFF321 (AFF321Q) /CSM/AF/AFF3-A/AFF321Q.Seq.d/ AGAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA TGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCA AATGGAGTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGT ACTACGTGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAAT TCAAGATGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCA AGTGGCACTTGAATATGCAACTCATTATCGTACCAATAATGGTACTAATTCCATTTTGTT TGGGATTTCGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGGACAAACTAAACGG ACATATATAAATCACXXXXXXXXXXGGTTCAACCAACCAGTGGTAATTATGTACCTGTGA ATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATA GAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTA CTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTA CAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTG CTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAA ACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTAC CAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTT TACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCA CTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATT TAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTA AAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTT TACTTTTTCTCCAATGCAAATCAGAACTTTTAAATTACTA
sequence update	2003. 5. 2
Translated Amino Acid sequence	rkkkphthkngnkkiiyinflfvfnyqnwkknknkykiktktiiniikcsyccsys cwlvknsillwikyvncicrcsiyfgyshylfigksrkkvylcncifpkmvgtk ynnakycgfgkwsigihkwwvlye*gyyllryypndigpsiplgefrsyakdrla hrsiwpcntssylwsirircihyrsyglsryrssfreangvhveidpkytressfhis ttcnvlysrwfqlar*pnsrpkfir*rrlkstvhssgticnslsyqwy*FHFV WDFAYLNAQMYYKNIGQTKRTYINH--- ---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC KSELLNY
Translated Amino Acid sequence (All Frames)	Frame A: rkkkphthkngnkkiiyinflfvfnyqnwkknknkykiktktiiniikcsyccsys cwlvknsillwikyvncicrcsiyfgyshylfigksrkkvylcncifpkmvgtk ynnakycgfgkwsigihkwwvlye*gyyllryypndigpsiplgefrsyakdrla hrsiwpcntssylwsirircihyrsyglsryrssfreangvhveidpkytressfhis ttcnvlysrwfqlar*pnsrpkfir*rrlkstvhssgticnslsyqwyFHFV WDFAYLNAQMYYKNIGQTKRTYINH--- ---gstnqwlctcecyclhsrssiitihhchkswmcivkrwsigyddassyferw swcwstnevnsnrynidfpyfklcsislsscctlfitsitpnvynhstifkrlefs isrclltidfsltftkwfkdsnstmvrsryhtftykylsnwsrftrstnyhfrsfn ylfnnynhfcnnefnwcskiikfikikmencwklsiiiinrrffkgfvftfspm qirtfkll Frame B: ekkshthikmvikklfilifclfliineingkktkindikkskpklsstllnvhivahth ddvgwlktvdeyyygsnmsiafagvqytldtaitcllanperkfiyveiaffqrwwdeqs ttmqnivkglvesgqlefinggycmndeattyyddtidqmtlghqflwenfgvmpkigwh idpfghsatqarifgqlgfdafiigrmdyqdiearlenkqmefmwrstqstpenqvftsv lramyctpdgfnfeqgddpiqddpnlfdnnvdsraeqftqvaleyathyrtnngtnsilf gisltltlkcitktldklnghii--- ---VQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLRDGQLDMMMHRRTLKDDG RGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPLLPMFTTTQQSSNDWNSQ YQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQIDGQDSQDPQTITLDLST IFSTITITSATEMNLTGVQKLSNLSRLKWKTVDGKNYDHKSSSSTKEDSSKVLFLLFLQC KSELLNY Frame C: kkkathtkw*knylyffvcflsmklmekkqkmilknqnqnyhqhymfilllilm mmlvgkqlmniimdqicqlhlqvfnilwiqplpvywqiqkeslfmlklhfskdggmnkv qqckillrvwlkvvnwnsmvgivmmrllptmtilltkhwainsfgriselcqragt ihlaivqhklvslvndsmhslsvvwiikisklvriskwsscgdrpkvhqrikfshqy yvqcivlqmvstlskvmtqfkmtqiysiittqelnsslkwhlnmqliivpimvlipfcl gfrlprsnvlqkhwtntdiyks--- ---fnqpvvimylmllptfkilinhynsplslievvdvhremvnwi*civvlkmmv vvlvnqmsqlkslqlvnfsmifqvmlnliivllhslyhihysqclqplnnlqtigiln ikvfthhlqphlyqmvrfklyngiikiipyfyvlkifiklmvkihkihklslifql sfqqlqsllqlkilvfknyqiyqdngkllmvktmiinhhhqlkkilqrfcfyffsna nqnfit
Homology vs CSM-cDNA	Score E Sequences producing significant alignments: (bits) Value AFF321 (AFF321Q) /CSM/AF/AFF3-A/AFF321Q.Seq.d/ 3081 0.0 VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/ 1457 0.0 VHK686 (VHK686Q) /CSM/VH/VHK6-D/VHK686Q.Seq.d/ 1457 0.0 VHI153 (VHI153Q) /CSM/VH/VHI1-C/VHI153Q.Seq.d/ 1457 0.0 VHH791 (VHH791Q) /CSM/VH/VHH7-D/VHH791Q.Seq.d/ 1457 0.0 VHE461 (VHE461Q) /CSM/VH/VHE4-C/VHE461Q.Seq.d/ 1457 0.0 VHE212 (VHE212Q) /CSM/VH/VHE2-A/VHE212Q.Seq.d/ 1457 0.0 VHB156 (VHB156Q) /CSM/VH/VHB1-C/VHB156Q.Seq.d/ 1457 0.0 SHC431 (SHC431Q) /CSM/SH/SHC4-B/SHC431Q.Seq.d/ 1457 0.0 AHE423 (AHE423Q) /CSM/AH/AHE4-A/AHE423Q.Seq.d/ 1457 0.0
own update	2004.12.25
Homology vs DNA	Score E Sequences producing significant alignments: (bits) Value N M82822\|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds. 1324 0.0 5 CK149800\|CK149800.1 CsmgEST00336 Culicoides sonorensis female serum-fed midgut cDNA Library Culicoides sonorensis cDNA, mRNA sequence. 117 2e-23 3 CB073433\|CB073433.1 taa32h01.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to TR:Q93093 Q93093 TRUNCATED LYSOSOMAL ACID ALPHA-MANNOSIDASE ;, mRNA sequence. 52 3e-15 4 BU798369\|BU798369.1 SJF2ADB01 SJF Schistosoma japonicum cDNA, mRNA sequence. 74 4e-14 3 BU798139\|BU798139.1 SJF2AAF09 SJF Schistosoma japonicum cDNA, mRNA sequence. 74 1e-13 3 BU804021\|BU804021.1 SJFBFG06 SJF Schistosoma japonicum cDNA, mRNA sequence. 74 4e-13 2 AW774908\|AW774908.1 EST334059 KV3 Medicago truncatula cDNA clone pKV3-24H19, mRNA sequence. 60 9e-12 3 AW587127\|AW587127.1 EST318750 MHAM Medicago truncatula/Glomus versiforme mixed EST library cDNA clone pMHAM-57O10, mRNA sequence. 60 2e-11 3 BM780126\|BM780126.1 EST590702 KV2 Medicago truncatula cDNA clone pKV2-54G5, mRNA sequence. 60 3e-11 3 AV957574\|AV957574.1 Ciona intestinalis cDNA, clone:cieg17m22, 5' end, single read. 58 1e-09 3
dna update	2004. 3.26
Homology vs Protein	Score E Sequences producing significant alignments: (bits) Value (P34098) RecName: Full=Lysosomal alpha-mannosidase; Sho... 520 e-149 M82822_1(M82822\|pid:none) Dictyostelium discoideum alpha-mannosi... 509 e-146 BC091843_1(BC091843\|pid:none) Danio rerio zgc:110815, mRNA (cDNA... 270 3e-71 AB026648_12(AB026648\|pid:none) Arabidopsis thaliana genomic DNA,... 270 1e-70 AY099704_1(AY099704\|pid:none) Arabidopsis thaliana alpha-mannosi... 264 6e-69 AB005230_20(AB005230\|pid:none) Arabidopsis thaliana genomic DNA,... 264 6e-69 AK068415_1(AK068415\|pid:none) Oryza sativa Japonica Group cDNA c... 261 4e-68 AC148217_12(AC148217\|pid:none) Medicago truncatula clone mth2-20... 260 1e-67 BC121882_1(BC121882\|pid:none) Xenopus tropicalis lysosomal alpha... 259 2e-67 (Q29451) RecName: Full=Lysosomal alpha-mannosidase; Sho... 256 2e-66
protein update	2009. 3.28
PSORT	psg: 0.75 gvh: 0.35 alm: 0.41 top: 0.53 tms: 0.00 mit: 0.26 mip: 0.05 nuc: 0.00 erl: 0.00 erm: 0.00 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00 act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00 dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00 m3a: 0.00 m3b: 0.00 m_ : 1.00 56.0 %: cytoplasmic 24.0 %: nuclear 12.0 %: mitochondrial 4.0 %: plasma membrane 4.0 %: vesicles of secretory system >> prediction for AFF321 is cyt
5' end seq. ID	AFF321F
5' end seq.	>AFF321F.Seq AGAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA TGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCA AATGGAGTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGT ACTACGTGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAAT TCAAGATGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCA AGTGGCACTTGAATATGCAACTCATTATCGTACCAATAATGGTACTAATTCCATTTTGTT TGGGATTTCGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGGACAAACTAAACGG ACATATATAAATCACNNNNNNNNNN
Length of 5' end seq.	985
3' end seq. ID	AFF321Z
3' end seq.	>AFF321Z.Seq ----------GGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACA TTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTG CATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATG GTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGA TTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTAT CACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTC AATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGA TTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATA TTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAA CTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAA AATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTAAAAACTATGATCATA AATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTTTACTTTTTCTCCAAT GCAAATCAGAACTTTTAAATTACTA
Length of 3' end seq.	735
Connected seq. ID	AFF321P
Connected seq.	>AFF321P.Seq AGAAAAAAAAAGCCACACACACATAAAAATGGTAATAAAAAAATTATTTATATTAATTTT TTGTTTGTTTTTAATTATCAATGAAATTAATGGAAAAAAAACAAAAATAAATGATATTAA AAAATCAAAACCAAAACTATCATCAACATTATTAAATGTTCATATTGTTGCTCATACTCA TGATGATGTTGGTTGGTTAAAAACAGTTGATGAATATTATTATGGATCAAATATGTCAAT TGCATTTGCAGGTGTTCAATATACTTTGGATACAGCCATTACCTGTTTATTGGCAAATCC AGAAAGAAAGTTTATTTATGTTGAAATTGCATTTTTCCAAAGATGGTGGGATGAACAAAG TACAACAATGCAAAATATTGTTAAGGGTTTGGTTGAAAGTGGTCAATTGGAATTCATAAA TGGTGGGTATTGTATGAATGATGAGGCTACTACCTACTATGACGATACTATTGACCAAAT GACATTGGGCCATCAATTCCTTTGGGAGAATTTCGGAGTTATGCCAAAGATAGGCTGGCA CATAGATCCATTTGGCCATAGTGCAACACAAGCTCGTATCTTTGGTCAATTAGGATTCGA TGCATTCATTATCGGTCGTATGGATTATCAAGATATCGAAGCTCGTTTAGAGAATAAGCA AATGGAGTTCATGTGGAGATCGACCCAAAGTACACCAGAGAATCAAGTTTTCACATCAGT ACTACGTGCAATGTATTGTACTCCAGATGGTTTCAACTTTGAGCAAGGTGATGACCCAAT TCAAGATGACCCAAATTTATTCGATAATAACGTAGACTCAAGAGCTGAACAGTTCACTCA AGTGGCACTTGAATATGCAACTCATTATCGTACCAATAATGGTACTAATTCCATTTTGTT TGGGATTTCGCTTACCTTAACGCTCAAATGTATTACAAAAACATTGGACAAACTAAACGG ACATATATAAATCAC----------GGTTCAACCAACCAGTGGTAATTATGTACCTGTGA ATGCTATTGCCTACATTCAAGATCCTAATCAATCATTACAATTCACCATTGTCACTGATA GAAGTCGTGGATGTGCATCGTTAAGAGATGGTCAATTGGATATGATGATGCATCGTCGTA CTTTGAAAGATGATGGTCGTGGTGTTGGTCAACCAATGAATGAGTCAACTCAAATCGTTA CAACTAGTAAATTGATTTTCCATGATATTTCAAGTTATGCTCAATCTCATTATCGTCCTG CTGCACTCTCTTTATCACATCCATTACTCCCAATGTTTACAACCACTCAACAATCTTCAA ACGATTGGAATTCTCAATATCAAGGTGTTTACTCACCATTGACTTCAGCCTCACCTTTAC CAAATGGTTTAAAGATTCAAACTCTACAATGGTTAGATAATCAAGATAATACCATACTTT TACGTATTGAAAATATTTATCAAATTGATGGTCAAGATTCACAAGATCCACAAACTATCA CTTTAGATCTTTCAACTATCTTTTCAACAATTACAATCACTTCTGCAACTGAAATGAATT TAACTGGTGTTCAAAAATTATCAAATTTATCAAGATTAAAATGGAAAACTGTTGATGGTA AAAACTATGATCATAAATCATCATCATCAACTAAAGAAGATTCTTCAAAGGTTTTGTTTT TACTTTTTCTCCAATGCAAATCAGAACTTTTAAATTACTA
Length of connected seq.	1710
Full length Seq ID	-
Full length Seq.	-
Length of full length seq.	-

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas