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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF313
Library AF
(Link to library)
Clone ID AFF313
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFF313P
(Link to Original site)
Representative DNA sequence 
>AFF313 (AFF313Q) /CSM/AF/AFF3-A/AFF313Q.Seq.d/
ATTTCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTC
AAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATG
CTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTG
GTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGNTCTCTTAA
CCCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAA
ACCTTNAACACCCCANCCATGTACGTTGCCATTCAAGCCGXXXXXXXXXXGAACAAAAAA
TTAACTGCTTTAGCNCCNTCAACCATGAAAATTNAAATCNTTGNNCCCCCANAACGNAAA
AACNCNGGNTGGANTGGNGGATCTATTTTAG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
is*nk*iklnkk*kwmvkmfkl*llitvlvcvkpvllvtmlhvlfshqllvvqdtlvlwl
vwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlhqkntqxs*
PEAPLNPKANREKMTQIMFETXNTPXMYVAIQA---

---EQKINCFSXXNHENXNXXXPXTXKXXXXWXIYF
Translated Amino Acid sequence (All Frames) 
Frame A:
is*nk*iklnkk*kwmvkmfkl*llitvlvcvkpvllvtmlhvlfshqllvvqdtlvlwl
vwvkkthm*vmkpnqrevs*psntqlntvslptgmiwkksgiilstmnsvlhqkntqxs*
PEAPLNPKANREKMTQIMFETXNTPXMYVAIQA---

---EQKINCFSXXNHENXNXXXPXTXKXXXXWXIYF

Frame B:
flktnkln*iknkngw*rcssfsy**rfwyv*srfcw*rcstccfpincwsskthwcygw
ygskrlicr**spikerylnpqipn*tryryqlg*ygknlasyflq*tpcctrrtpxsln
pklh*iqkptekk*pklclkpxtpxpctlpfkp---

---nkkltalapstmkixixxppxrknxgwxggsil

Frame C:
flkqin*ik*kikmdgedvqalvidngsgmckagfagddapravfpsivgrprhtgvmvg
mgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelrvapeehpxllt
rssiksksqqrkndpnyv*nlxhpxhvrchss---

---tkn*ll*xxqp*kxkslxpxnxktxxgxxdlf*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF313 (AFF313Q) /CSM/AF/AFF3-A/AFF313Q.Seq.d/                        892   0.0
VFM527 (VFM527Q) /CSM/VF/VFM5-B/VFM527Q.Seq.d/                        793   0.0
VFL353 (VFL353Q) /CSM/VF/VFL3-C/VFL353Q.Seq.d/                        793   0.0
VFL311 (VFL311Q) /CSM/VF/VFL3-A/VFL311Q.Seq.d/                        793   0.0
VFK419 (VFK419Q) /CSM/VF/VFK4-A/VFK419Q.Seq.d/                        793   0.0
VFK366 (VFK366Q) /CSM/VF/VFK3-C/VFK366Q.Seq.d/                        793   0.0
VFK110 (VFK110Q) /CSM/VF/VFK1-A/VFK110Q.Seq.d/                        793   0.0
VFJ294 (VFJ294Q) /CSM/VF/VFJ2-D/VFJ294Q.Seq.d/                        793   0.0
VFI680 (VFI680Q) /CSM/VF/VFI6-D/VFI680Q.Seq.d/                        793   0.0
SLH494 (SLH494Q) /CSM/SL/SLH4-D/SLH494Q.Seq.d/                        793   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   555   0.0    6
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         626   0.0    3
AC115680|AC115680.3 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   626   0.0    4
U25660|U25660.1 Dictyostelium discoideum actin gene, partial cds.   618   0.0    2
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.       577   0.0    3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   569   0.0    7
AC116963|AC116963.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   507   0.0    7
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   500   e-175  4
X03282|X03282.1 dictyostelium discoideum gene for actin A12.      476   e-170  3
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   490   e-164  3
dna update 2004. 5. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   225   3e-70
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   225   3e-70
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   223   9e-70
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   223   1e-69
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        220   8e-69
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   220   2e-68
AY280960_1(AY280960|pid:none) Homo sapiens actin alpha 1 skeleta...   204   2e-68
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   218   3e-68
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   218   4e-68
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   217   8e-68
protein update 2009. 4.19
PSORT 

 psg: 0.69  gvh: 0.32  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	 12.0 %: cytoskeletal

>> prediction for AFF313 is cyt
5' end seq. ID AFF313F
5' end seq. 
>AFF313F.Seq
ATTTCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTC
AAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATG
CTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTG
GTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGNTCTCTTAA
CCCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAA
ACCTTNAACACCCCANCCATGTACGTTGCCATTCAAGCCG----------
Length of 5' end seq. 460
3' end seq. ID AFF313Z
3' end seq. 
>AFF313Z.Seq
----------GAACAAAAAATTAACTGCTTTAGCNCCNTCAACCATGAAAATTNAAATCN
TTGNNCCCCCANAACGNAAAAACNCNGGNTGGANTGGNGGATCTATTTTAG
Length of 3' end seq. 101
Connected seq. ID AFF313P
Connected seq. 
>AFF313P.Seq
ATTTCTTAAAACAAATAAATTAAATTAAATAAAAAATAAAAATGGATGGTGAAGATGTTC
AAGCTTTAGTTATTGATAACGGTTCTGGTATGTGTAAAGCCGGTTTTGCTGGTGACGATG
CTCCACGTGCTGTTTTCCCATCAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTG
GTATGGGTCAAAAAGACTCATATGTAGGTGATGAAGCCCAATCAAAGAGAGGTATCTTAA
CCCTCAAATACCCAATTGAACACGGTATCGTTACCAACTGGGATGATATGGAAAAAATCT
GGCATCATACTTTCTACAATGAACTCCGTGTTGCACCAGAAGAACACCCAGNTCTCTTAA
CCCGAAGCTCCATTAAATCCAAAAGCCAACAGAGAAAAAATGACCCAAATTATGTTTGAA
ACCTTNAACACCCCANCCATGTACGTTGCCATTCAAGCCG----------GAACAAAAAA
TTAACTGCTTTAGCNCCNTCAACCATGAAAATTNAAATCNTTGNNCCCCCANAACGNAAA
AACNCNGGNTGGANTGGNGGATCTATTTTAG
Length of connected seq. 561
Full length Seq ID -
Full length Seq. -
Length of full length seq. -