>AFF294 (AFF294Q) /CSM/AF/AFF2-D/AFF294Q.Seq.d/
XXXXXXXXXXCAGTGGGTGTTAATGATAGTGAACAAGCTGAGGTCTATAAACGTGCCTTC
AATGGTGACCCAAAATCTGCCTATGGTGGTATCTTGGGTTTCAATAGAACTTTAACATTA
GAGACTGCAACTGCATTGAAAAGTGTTTTCTATGAAGTTATCATCGCACCAGATTACACC
GAAGATGCATTAGCATTGTTAAGTAAAAAAGAGAAATTACGTATTCTTCGTATACCAGAG
GCCGCCAATCAAATTCAATTCACTCAACCTGATATTCGTACCATCACTGGTGGTGCTTTA
CTTCAATCTCCAAATCCAATCATCAGAGGTGACCTTGCTGAAGCTACAAAGAATTGGAAG
GTCGTCACTGAAAATAAACCAACTGAACAACAAATGAAAGATCTCTTATTCGCTTGGAGA
GTTTCAAAACATGTTAAATCAAATGCTATCGTTTTATCAAAGGATGAAACTATCGTTGCC
ATTGGTGCTGGTCAACCAAATCGTTCACAATCTGTTGACATTTGTATGAAAGTTGGTGGT
GATAAAGTTAAAGGAAGTGTTTTGGCTTCTGATGCTTTCTTCCCATTCGCCGATTCAATC
GATTTAGCT

---VGVNDSEQAEVYKRAFNGDPKSAYGGILGFNRTLTLETATALKSVFYEVIIAPDYTE
DALALLSKKEKLRILRIPEAANQIQFTQPDIRTITGGALLQSPNPIIRGDLAEATKNWKV
VTENKPTEQQMKDLLFAWRVSKHVKSNAIVLSKDETIVAIGAGQPNRSQSVDICMKVGGD
KVKGSVLASDAFFPFADSIDLA

Frame A:
---sgc****ts*gl*tclqw*pkiclwwylgfq*nfnirdcnciekcfl*syhrtrlhr
rcisivk*kreityssytrgrqsnsihst*ysyhhwwcftsisksnhqr*pc*sykeleg
rh*k*tn*ttnerslirlesfktc*ikcyrfikg*nyrchwcwstksftic*hlyesww*
*s*rkcfgf*cflpirrfnrfs


Frame B:
---VGVNDSEQAEVYKRAFNGDPKSAYGGILGFNRTLTLETATALKSVFYEVIIAPDYTE
DALALLSKKEKLRILRIPEAANQIQFTQPDIRTITGGALLQSPNPIIRGDLAEATKNWKV
VTENKPTEQQMKDLLFAWRVSKHVKSNAIVLSKDETIVAIGAGQPNRSQSVDICMKVGGD
KVKGSVLASDAFFPFADSIDLA


Frame C:
---wvlmivnklrsinvpsmvtqnlpmvvswvsiel*h*rlqlh*kvfsmklsshqitpk
mh*hc*vkkrnyvffvyqrppikfnslnlifvpslvvlyfnlqiqssevtllklqrigrs
slkinqlnnk*kisyslgefqnmlnqmlsfyqrmklslplvlvnqivhnlltfv*klvvi
klkevfwllmlsshspiqsi*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFG811 (AFG811Q) /CSM/AF/AFG8-A/AFG811Q.Seq.d/                       1187   0.0
AFF294 (AFF294Q) /CSM/AF/AFF2-D/AFF294Q.Seq.d/                       1187   0.0
CFG295 (CFG295Q) /CSM/CF/CFG2-D/CFG295Q.Seq.d/                       1150   0.0
SFI238 (SFI238Q) /CSM/SF/SFI2-B/SFI238Q.Seq.d/                       1126   0.0
SFF145 (SFF145Q) /CSM/SF/SFF1-B/SFF145Q.Seq.d/                       1122   0.0
VFJ414 (VFJ414Q) /CSM/VF/VFJ4-A/VFJ414Q.Seq.d/                       1112   0.0
VFM711 (VFM711Q) /CSM/VF/VFM7-A/VFM711Q.Seq.d/                       1098   0.0
SFI552 (SFI552Q) /CSM/SF/SFI5-C/SFI552Q.Seq.d/                       1094   0.0
CHB202 (CHB202Q) /CSM/CH/CHB2-A/CHB202Q.Seq.d/                       1090   0.0
SFI262 (SFI262Q) /CSM/SF/SFI2-C/SFI262Q.Seq.d/                       1086   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.  1187   0.0    1
AE013028|AE013028.1 Thermoanaerobacter tengcongensis strain MB4T, section 55 of 244 of the complete genome.    40   0.022  2
CD148667|CD148667.1 ML1-0013T-L094-B01-U.B ML1-0013 Schistosoma mansoni cDNA clone ML1-0013T-L094-B01.B, mRNA sequence.    34   0.51   2
AC117638|AC117638.13 Mus musculus, clone RP23-225L9, complete sequence.    46   0.60   1
AZ428384|AZ428384.1 1M0210C20R Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0210C20 R, DNA sequence.    46   0.60   1
AC113486|AC113486.10 Mus musculus chromosome 14 clone RP23-354M3 map 14, *** SEQUENCING IN PROGRESS ***, 2 ordered pieces.    46   0.60   1
AC120872|AC120872.2 Mus musculus chromosome 14 clone RP23-255I2 map 14, *** SEQUENCING IN PROGRESS ***, 18 unordered pieces.    46   0.60   1
U92221|U92221.1 Legionella shakespearei macrophage infectivity potentiator (mip) gene, complete cds.    46   0.60   1
AF022351|AF022351.1 Legionella sp. G macrophage infectivity potentiator protein (mip) gene, partial cds.    46   0.60   1
AY050839|AY050839.1 Arabidopsis thaliana unknown protein (At5g02100) mRNA, complete cds.    32   1.3    3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ965256_1146(AJ965256|pid:none) Dehalococcoides sp. CBDB1 compl...   184   2e-45
CP000027_1370(CP000027|pid:none) Dehalococcoides ethenogenes 195...   182   6e-45
CP000924_1673(CP000924|pid:none) Thermoanaerobacter pseudethanol...   171   2e-41
(A4XKZ2) RecName: Full=Bifunctional purine biosynthesis protein ...   169   7e-41
CP001393_1377(CP001393|pid:none) Anaerocellum thermophilum DSM 6...   167   3e-40
CP001037_5786(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...   166   3e-40
(Q8RC55) RecName: Full=Bifunctional purine biosynthesis protein ...   166   5e-40
CP001287_1273(CP001287|pid:none) Cyanothece sp. PCC 8801, comple...   166   6e-40
(Q8YSJ2) RecName: Full=Bifunctional purine biosynthesis protein ...   165   1e-39
CP001291_3523(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...   164   2e-39

 psg: 0.75  gvh: 0.36  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFF294 is cyt

>AFF294Z.Seq
----------CAGTGGGTGTTAATGATAGTGAACAAGCTGAGGTCTATAAACGTGCCTTC
AATGGTGACCCAAAATCTGCCTATGGTGGTATCTTGGGTTTCAATAGAACTTTAACATTA
GAGACTGCAACTGCATTGAAAAGTGTTTTCTATGAAGTTATCATCGCACCAGATTACACC
GAAGATGCATTAGCATTGTTAAGTAAAAAAGAGAAATTACGTATTCTTCGTATACCAGAG
GCCGCCAATCAAATTCAATTCACTCAACCTGATATTCGTACCATCACTGGTGGTGCTTTA
CTTCAATCTCCAAATCCAATCATCAGAGGTGACCTTGCTGAAGCTACAAAGAATTGGAAG
GTCGTCACTGAAAATAAACCAACTGAACAACAAATGAAAGATCTCTTATTCGCTTGGAGA
GTTTCAAAACATGTTAAATCAAATGCTATCGTTTTATCAAAGGATGAAACTATCGTTGCC
ATTGGTGCTGGTCAACCAAATCGTTCACAATCTGTTGACATTTGTATGAAAGTTGGTGGT
GATAAAGTTAAAGGAAGTGTTTTGGCTTCTGATGCTTTCTTCCCATTCGCCGATTCAATC
GATTTAGCT