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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF287
Library AF
(Link to library)
Clone ID AFF287
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16368-1
Original site URL
Representative seq. ID AFF287P
(Link to Original site)
Representative DNA sequence 
>AFF287 (AFF287Q) /CSM/AF/AFF2-D/AFF287Q.Seq.d/
TGGGAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGAC
GGTTGTGGTAATATTCAATGTCCATGGGGCCACTATTGTGTCAATGAACATGGTAAATGC
AGATGTGTACCACACAGACCACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACAT
TGTCCACATGGTTATTCATGTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCC
CGTCCACCACATAACCTTTGTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTT
CTTGAAAAACATCCAGTTTGTGTTAGAAATCATGTTCCACCACACCCACCACCACCACCA
CAAATTTGCGGTAGTGTAAATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCAT
CCAACTTGTATTCGTGGTGATGGTTATTTATGTAATCAAACCCGTTGTCCACACGATTAT
CAATGTGAAACCATTAGCACCAATATCGTGAAATGTTCACCAAAGAAXXXXXXXXXXGTT
CACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATA
GTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTTGTAAAGTTACTC
AATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAA
GACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATC
ACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCATGTT
GCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCAACAA
CTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAA
CTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTG
CCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTA
GCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCTCAA
GTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGCTCAGAAA
GCTCAGAAAGCTCATCAGCCACTTCA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
WEGFYCEEQGGSACCVPHHDGCGNIQCPWGHYCVNEHGKCRCVPHRPPPRPPVDQCRNQH
CPHGYSCRVIKGCATCVRDARPPHNLCRGFGCPEGSHCEVLEKHPVCVRNHVPPHPPPPP
QICGSVNCGPGYICTIINGHPTCIRGDGYLCNQTRCPHDYQCETISTNIVKCSPK---

---SPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTC
IKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAAST
IATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAASSAASSE
PSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSATS
Translated Amino Acid sequence (All Frames) 
Frame A:
WEGFYCEEQGGSACCVPHHDGCGNIQCPWGHYCVNEHGKCRCVPHRPPPRPPVDQCRNQH
CPHGYSCRVIKGCATCVRDARPPHNLCRGFGCPEGSHCEVLEKHPVCVRNHVPPHPPPPP
QICGSVNCGPGYICTIINGHPTCIRGDGYLCNQTRCPHDYQCETISTNIVKCSPK---

---vhqrmtnvngivvhqvpaasivetvhtvlpimyshnfvkllnvqlislvk*leviql
vlkqdhryhhhiaqlvqsyhqhvitle*fvfkyqaivpildshvahliqfvfihqllllq
plqqlhqlsqlqplqllqvqqlvelqlvvqllivvllpqpivalpphhhqavllqvplqv
nhqvaplqvvlhpllqvalhplpqvvlhhhqlqvqlhhqlqaqkaqkahqpl

Frame B:
gkdsivknkvvvhaayhimtvvvifnvhgativsmnmvnadvyhtdhhhvhqltnavini
vhmvihvvslkvvplvsempvhhitfvvvlvvqkapivkflkniqfvleimfhhthhhhh
kfavv*ivvqvifvqlsmviqlvfvvmviyvikpvvhtiinvkplapis*nvhqr---

---ftke*rm*maslstrfqllq**krstlscq*cipttl*sysmsn*fll*nd*r*snl
y*skttgttttllnlcraiism*srwndlysstkqlyqy*ipmlpissnlyssinyccfn
hcnncincrnynlcnycrynnwwnynwwfnf***ccflsr**rcllitikqccfkcrfk*
tik*rrfk*csilcfk*rsilclk*csiiisfkfsciiscklrklrklishf

Frame C:
grill*rtrw*cmlrtts*rlw*ysmsmgpllcq*tw*mqmcttqttttsts*pmp*stl
stwlfmsch*rlchlcqrcpstt*plswfwlsrrlpl*ss*ktsslc*kscsttpttttt
nlr*cklwsrlylynyqwssnlysw*wlfm*snplstrlsm*nh*hqyremftke---

---SPKNDECKWHRCPPGSSCFNSRNGPHCLANNVFPQLCKVTQCPTDFSCKMIRGNPTC
IKARPPVPPPHCSTCAELSSACNHVGMICIQVPSNCTNTRFPCCPSHPICIHPSTTAAST
IATTASTVATTTSATTAGTTTGGTTTGGSTSDSSAASSADSSAASSSPSSSAASSAASSE
PSSSAASSSAPSSASSSAPSSASSSAPSSSASSSAASSAASSESSESSSATS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SLB244 (SLB244Q) /CSM/SL/SLB2-B/SLB244Q.Seq.d/                       1366   0.0
SLB171 (SLB171Q) /CSM/SL/SLB1-C/SLB171Q.Seq.d/                       1366   0.0
SLB132 (SLB132Q) /CSM/SL/SLB1-B/SLB132Q.Seq.d/                       1366   0.0
SLA236 (SLA236Q) /CSM/SL/SLA2-B/SLA236Q.Seq.d/                       1366   0.0
SHA862 (SHA862Q) /CSM/SH/SHA8-C/SHA862Q.Seq.d/                       1366   0.0
SFL452 (SFL452Q) /CSM/SF/SFL4-C/SFL452Q.Seq.d/                       1366   0.0
SFL286 (SFL286Q) /CSM/SF/SFL2-D/SFL286Q.Seq.d/                       1366   0.0
SFL239 (SFL239Q) /CSM/SF/SFL2-B/SFL239Q.Seq.d/                       1366   0.0
SFL185 (SFL185Q) /CSM/SF/SFL1-D/SFL185Q.Seq.d/                       1366   0.0
SFK792 (SFK792Q) /CSM/SF/SFK7-D/SFK792Q.Seq.d/                       1366   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D13973|D13973.1 Dictyostelium discoideum DNA for Dp87 protein, complete cds.  1366   0.0    5
BJ170760|BJ170760.1 Physcomitrella patens subsp. patens cDNA clone:pph26m16, 3' end, single read.   190   3e-44  1
AF279135|AF279135.1 Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds.    64   5e-06  1
AC099379|AC099379.5 Rattus norvegicus clone CH230-42H5, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    42   0.42   7
C26876|C26876.1 Oryza sativa (japonica cultivar-group) cDNA, partial sequence (C50317_1A).    36   0.74   2
AC125101|AC125101.3 Mus musculus BAC clone RP24-207F9 from chromosome 19, complete sequence.    40   1.3    7
CN187496|CN187496.1 UCRCS05_0004I21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0004I21, mRNA sequence.    34   2.4    3
AK100095|AK100095.1 Oryza sativa (japonica cultivar-group) cDNA clone:J023004B12, full insert sequence.    36   4.4    2
AE017095|AE017095.1 Oryza sativa (japonica cultivar-group) chromosome 10, section 49 of 77 of the complete sequence.    44   4.9    1
AL110484|AL110484.2 Caenorhabditis elegans YAC Y38E10A.            44   4.9    1
dna update 2004. 5. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;       330   8e-89
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...   330   8e-89
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    77   3e-19
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    65   6e-13
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    74   1e-11
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    56   2e-11
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    49   1e-10
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    70   1e-10
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    67   9e-10
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    49   3e-04
protein update 2009. 4.19
PSORT 

 psg: 0.75  gvh: 0.28  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFF287 is nuc
5' end seq. ID AFF287F
5' end seq. 
>AFF287F.Seq
TGGGAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGAC
GGTTGTGGTAATATTCAATGTCCATGGGGCCACTATTGTGTCAATGAACATGGTAAATGC
AGATGTGTACCACACAGACCACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACAT
TGTCCACATGGTTATTCATGTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCC
CGTCCACCACATAACCTTTGTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTT
CTTGAAAAACATCCAGTTTGTGTTAGAAATCATGTTCCACCACACCCACCACCACCACCA
CAAATTTGCGGTAGTGTAAATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCAT
CCAACTTGTATTCGTGGTGATGGTTATTTATGTAATCAAACCCGTTGTCCACACGATTAT
CAATGTGAAACCATTAGCACCAATATCGTGAAATGTTCACCAAAGAA----------
Length of 5' end seq. 527
3' end seq. ID AFF287Z
3' end seq. 
>AFF287Z.Seq
----------GTTCACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCC
AGCTGCTTCAATAGTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTT
TGTAAAGTTACTCAATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACT
TGTATTAAAGCAAGACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCA
TCAGCATGTAATCACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACT
AGATTCCCATGTTGCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCA
ACCATTGCAACAACTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACA
ACAACTGGTGGAACTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCC
GATAGTAGCGCTGCCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGT
GAACCATCAAGTAGCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCA
TCCTCTGCCTCAAGTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCT
GCAAGCTCAGAAAGCTCAGAAAGCTCATCAGCCACTTCA
Length of 3' end seq. 689
Connected seq. ID AFF287P
Connected seq. 
>AFF287P.Seq
TGGGAAGGATTCTATTGTGAAGAACAAGGTGGTAGTGCATGCTGCGTACCACATCATGAC
GGTTGTGGTAATATTCAATGTCCATGGGGCCACTATTGTGTCAATGAACATGGTAAATGC
AGATGTGTACCACACAGACCACCACCACGTCCACCAGTTGACCAATGCCGTAATCAACAT
TGTCCACATGGTTATTCATGTCGTGTCATTAAAGGTTGTGCCACTTGTGTCAGAGATGCC
CGTCCACCACATAACCTTTGTCGTGGTTTTGGTTGTCCAGAAGGCTCCCATTGTGAAGTT
CTTGAAAAACATCCAGTTTGTGTTAGAAATCATGTTCCACCACACCCACCACCACCACCA
CAAATTTGCGGTAGTGTAAATTGTGGTCCAGGTTATATTTGTACAATTATCAATGGTCAT
CCAACTTGTATTCGTGGTGATGGTTATTTATGTAATCAAACCCGTTGTCCACACGATTAT
CAATGTGAAACCATTAGCACCAATATCGTGAAATGTTCACCAAAGAA----------GTT
CACCAAAGAATGACGAATGTAAATGGCATCGTTGTCCACCAGGTTCCAGCTGCTTCAATA
GTAGAAACGGTCCACACTGTCTTGCCAATAATGTATTCCCACAACTTTGTAAAGTTACTC
AATGTCCAACTGATTTCTCTTGTAAAATGATTAGAGGTAATCCAACTTGTATTAAAGCAA
GACCACCGGTACCACCACCACATTGCTCAACTTGTGCAGAGCTATCATCAGCATGTAATC
ACGTTGGAATGATTTGTATTCAAGTACCAAGCAATTGTACCAATACTAGATTCCCATGTT
GCCCATCTCATCCAATTTGTATTCATCCATCAACTACTGCTGCTTCAACCATTGCAACAA
CTGCATCAACTGTCGCAACTACAACCTCTGCAACTACTGCAGGTACAACAACTGGTGGAA
CTACAACTGGTGGTTCAACTTCTGATAGTAGTGCTGCTTCCTCAGCCGATAGTAGCGCTG
CCTCCTCATCACCATCAAGCAGTGCTGCTTCAAGTGCCGCTTCAAGTGAACCATCAAGTA
GCGCCGCTTCAAGTAGTGCTCCATCCTCTGCTTCAAGTAGCGCTCCATCCTCTGCCTCAA
GTAGTGCTCCATCATCATCAGCTTCAAGTTCAGCTGCATCATCAGCTGCAAGCTCAGAAA
GCTCAGAAAGCTCATCAGCCACTTCA
Length of connected seq. 1216
Full length Seq ID -
Full length Seq. -
Length of full length seq. -