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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF281
Library AF
(Link to library)
Clone ID AFF281
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14983-1
Original site URL
Representative seq. ID AFF281Z
(Link to Original site)
Representative DNA sequence 
>AFF281 (AFF281Q) /CSM/AF/AFF2-D/AFF281Q.Seq.d/
XXXXXXXXXXCAATAGTAATAATAATAATAATAATAATAATNCCAGAGGATNTTCAACCT
ACTAACCCAAGGTAATCAATCACATGGATTTGTTAGACCATCAATAGCAAATGGTTTTTG
CANCAACTGAATTTAATAATCATACCAATACCAATACCAATAGTAATAATAATAATAATA
ATAATAATTTCAATAATAACATAAAACCACCAACACCAGTTCAAACAAAGAGTAATGTAT
CATTAATTGATGACCAATGAATAATGGTATGATGAACAATGGTGGCATGATGAATAATGG
TGGTGGTGGTATGATGCCAATGAATAATGGCGGTATGATGCCAATGAATAATGGTGGTAT
GATGAACAATGGTGGTATGTTGAATAATGGTGTTATGATGAACAATGGTGGTATGATGCC
AATGAATAATGGAGGTATGATGGGAGGATTTGGTGGTATGATGCCAATGAATAATGGTGG
TATGATGGGTGGTGCTAACAATATGGGTGGCATGAATTTAGGAATGAATGCTATGTCTCC
AATGGGTGGTGCNCCAATGAATAACAATATGGGCGCTATGAATTTAGGAATGAATGCTAT
GTCTCCAATGGGTGGTGCTCCAATGAGGTCACCAGCAGCTGCTGGAAATAACTCTTTCAA
TATTACTCCAACTTCTGCTACTCCAACTCCACCACAACCATCTCAATTCTCTTCTTTGGA
TTCTTTAATGTTTACTTCATCCCCATCTCAACCAAAACAATCACAACCATCTCAATCATT
TGGTAACAATTTCCCAATCACTGCAAATAATTCAAATAACAACAATAATGTTAATAGCTA
TTCTTTAATAAAANTTACAANA
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---*********xqrxfnlltqgnqshgfvrpsiangfcxn*i**syqyqyq********
*fq**hkttntssnke*ciin**PMNNGMMNNGGMMNNGGGGMMPMNNGGMMPMNNGGMM
NNGGMLNNGVMMNNGGMMPMNNGGMMGGFGGMMPMNNGGMMGGANNMGGMNLGMNAMSPM
GGAPMNNNMGAMNLGMNAMSPMGGAPMRSPAAAGNNSFNITPTSATPTPPQPSQFSSLDS
LMFTSSPSQPKQSQPSQSFGNNFPITANNSNNNNNVNSYSLIKXTX
Translated Amino Acid sequence (All Frames) 
Frame A:
---nsnnnnnnnnxrgxsty*pkvinhmdlldhq*qmvfaxtefnnhtntntnsnnnnnn
nnfnnnikpptpvqtksnvsliddq*imv**tmva**imvvvv*cq*imav*cq*imvv*
*tmvvc*imvl**tmvv*cq*imev*wedlvv*cq*imvv*wvvltiwva*i*e*mlclq
wvvxq*itiwal*i*e*mlclqwvvlq*ghqqlleitlsillqllllqlhhnhlnsllwi
l*cllhphlnqnnhnhlnhlvtisqslqiiqittimliail**xlq


Frame B:
---iviiiiiiixpedxqptnpr*sitwic*tinskwflxqlnliiipipipiviiiiii
iisiit*nhqhqfkqrvmyh*lmtne*wydeqwwhde*wwwwydane*wrydane*wwyd
eqwwyve*wcydeqwwydane*wrydgriwwydane*wwydgwc*qygwhefrnecyvsn
gwcxne*qygryefrnecyvsngwcsnevtsscwk*lfqyysnfcysnstttisilffgf
fnvyfipistktittisiiw*qfpnhck*fk*qq*c**lffnkxyx


Frame C:
---*********xqrxfnlltqgnqshgfvrpsiangfcxn*i**syqyqyq********
*fq**hkttntssnke*ciin**PMNNGMMNNGGMMNNGGGGMMPMNNGGMMPMNNGGMM
NNGGMLNNGVMMNNGGMMPMNNGGMMGGFGGMMPMNNGGMMGGANNMGGMNLGMNAMSPM
GGAPMNNNMGAMNLGMNAMSPMGGAPMRSPAAAGNNSFNITPTSATPTPPQPSQFSSLDS
LMFTSSPSQPKQSQPSQSFGNNFPITANNSNNNNNVNSYSLIKXTX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF281 (AFF281Q) /CSM/AF/AFF2-D/AFF281Q.Seq.d/                       1066   0.0
AFI734 (AFI734Q) /CSM/AF/AFI7-B/AFI734Q.Seq.d/                        979   0.0
CFH641 (CFH641Q) /CSM/CF/CFH6-B/CFH641Q.Seq.d/                        295   6e-79
CHE182 (CHE182Q) /CSM/CH/CHE1-D/CHE182Q.Seq.d/                        113   5e-24
AHO116 (AHO116Q) /CSM/AH/AHO1-A/AHO116Q.Seq.d/                         44   0.004
AHM830 (AHM830Q) /CSM/AH/AHM8-B/AHM830Q.Seq.d/                         44   0.004
AHH391 (AHH391Q) /CSM/AH/AHH3-D/AHH391Q.Seq.d/                         44   0.004
VSI371 (VSI371Q) /CSM/VS/VSI3-C/VSI371Q.Seq.d/                         40   0.055
AHG836 (AHG836Q) /CSM/AH/AHG8-B/AHG836Q.Seq.d/                         38   0.22
AHG527 (AHG527Q) /CSM/AH/AHG5-B/AHG527Q.Seq.d/                         38   0.22
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.  1031   0.0    11
CE370632|CE370632.1 tigr-gss-dog-17000362055162 Dog Library Canis familiaris genomic, genomic survey sequence.    52   0.014  1
CE085313|CE085313.1 tigr-gss-dog-17000359007470 Dog Library Canis familiaris genomic, genomic survey sequence.    52   0.014  1
AC140969|AC140969.2 Canis familiaris clone RP81-197A18, WORKING DRAFT SEQUENCE, 11 ordered pieces.    52   0.014  1
BG138746|BG138746.1 EST479188 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP10N6 5' sequence, mRNA sequence.    38   0.031  2
AC113216|AC113216.4 Rattus norvegicus clone CH230-278J15, *** SEQUENCING IN PROGRESS ***, 32 unordered pieces.    38   0.51   4
AW737094|AW737094.1 EST338521 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD2E1 5', mRNA sequence.    38   0.51   2
AW737942|AW737942.1 EST339369 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD4B20 5', mRNA sequence.    38   0.52   2
AZ930230|AZ930230.1 474.dhz51b11.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomyces unisporus genomic clone 474.dhz51b11.s1, DNA sequence.    46   0.86   1
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    34   1.5    7
dna update 2004. 5. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000099_1947(CP000099|pid:none) Methanosarcina barkeri str. Fus...    43   0.011
CP001033_1257(CP001033|pid:none) Streptococcus pneumoniae CGSP14...    37   1.0
AJ870414_1(AJ870414|pid:none) Streptococcus pneumoniae lytB gene...    34   6.7
AJ870413_1(AJ870413|pid:none) Streptococcus pneumoniae lytB gene...    34   6.7
(P59205) RecName: Full=Putative endo-beta-N-acetylglucosaminidas...    34   6.7
CP001033_941(CP001033|pid:none) Streptococcus pneumoniae CGSP14,...    34   6.7
CP000936_976(CP000936|pid:none) Streptococcus pneumoniae Hungary...    33   8.8
(P59206) RecName: Full=Putative endo-beta-N-acetylglucosaminidas...    33   8.8
AE007317_867(AE007317|pid:none) Streptococcus pneumoniae R6, com...    33   8.8
protein update 2009. 4.19
PSORT 

 psg: 0.75  gvh: 0.12  alm: 0.53  top: 0.53  tms: 0.00  mit: -0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AFF281 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFF281Z
3' end seq. 
>AFF281Z.Seq
----------CAATAGTAATAATAATAATAATAATAATAATNCCAGAGGATNTTCAACCT
ACTAACCCAAGGTAATCAATCACATGGATTTGTTAGACCATCAATAGCAAATGGTTTTTG
CANCAACTGAATTTAATAATCATACCAATACCAATACCAATAGTAATAATAATAATAATA
ATAATAATTTCAATAATAACATAAAACCACCAACACCAGTTCAAACAAAGAGTAATGTAT
CATTAATTGATGACCAATGAATAATGGTATGATGAACAATGGTGGCATGATGAATAATGG
TGGTGGTGGTATGATGCCAATGAATAATGGCGGTATGATGCCAATGAATAATGGTGGTAT
GATGAACAATGGTGGTATGTTGAATAATGGTGTTATGATGAACAATGGTGGTATGATGCC
AATGAATAATGGAGGTATGATGGGAGGATTTGGTGGTATGATGCCAATGAATAATGGTGG
TATGATGGGTGGTGCTAACAATATGGGTGGCATGAATTTAGGAATGAATGCTATGTCTCC
AATGGGTGGTGCNCCAATGAATAACAATATGGGCGCTATGAATTTAGGAATGAATGCTAT
GTCTCCAATGGGTGGTGCTCCAATGAGGTCACCAGCAGCTGCTGGAAATAACTCTTTCAA
TATTACTCCAACTTCTGCTACTCCAACTCCACCACAACCATCTCAATTCTCTTCTTTGGA
TTCTTTAATGTTTACTTCATCCCCATCTCAACCAAAACAATCACAACCATCTCAATCATT
TGGTAACAATTTCCCAATCACTGCAAATAATTCAAATAACAACAATAATGTTAATAGCTA
TTCTTTAATAAAANTTACAANA
Length of 3' end seq. 852
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -