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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHA471
Library CH
(Link to library)
Clone ID CHA471
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHA471P
(Link to Original site)
Representative DNA sequence 
>CHA471 (CHA471Q) /CSM/CH/CHA4-C/CHA471Q.Seq.d/
CACTGTTGGCCTACTGGATTATTAAAAAAATGTACTCAGNAAATTATTGAAGATGAGGGG
ATGTATACATCTCCAAGAAAAAGAGATAATAATACTGATGATAAAGTTAGTTTAATTAAT
AAAGATAGTATTAGAAATAGTAATGACAGTATTTCATCGNCATCATCATCGTCATCAAAT
GATGATGATCATAATGNACXXXXXXXXXXTTCGTCCAATCTGTAAGAAGTTTATGAACAA
TCCATTGGAAATNTACATCAATGATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATA
CTACGTACCAATCACTGAAGAACAAAAGAATAAAAAACTCATTGAACTCTTGGATTCATT
AGACTTTAATCAAGCCGTTATTTTCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAA
GATCCTCCAAGATATTGGTTTCCCATCCATCTGTATTCATCGTGACCTCGATCAAAAGGA
TCGTATCGAACAATACCGTAAATTCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAA
TATCTTTGGCCGTGGTATCGATATTGAACGTGTCAATGTCGTCATCAACTACGATATGGC
TGAATCTGC
sequence update 2002.10.25
Translated Amino Acid sequence 
HCWPTGLLKKCTQXIIEDEGMYTSPRKRDNNTDDKVSLINKDSIRNSNDSISSXSSSSSN
DDDHNX---

---RPICKKFMNNPLEXYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVI
FVKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGID
IERVNVVINYDMAES
Translated Amino Acid sequence (All Frames) 
Frame A:
HCWPTGLLKKCTQXIIEDEGMYTSPRKRDNNTDDKVSLINKDSIRNSNDSISSXSSSSSN
DDDHNX---

---fvqsvrsl*tihwkxtsmmvvnslsmvfnnttyqslknkriknslnswih*tlikpl
fssnlldvlmp*irsskilvshpsvfivtsikrivsnntvnsktlnlvswlplislavvs
ilnvsmsssttiwlnl

Frame B:
tvglldy*knvlxkllkmrgcihlqekeiiilmiklv*likivleivmtvfhrhhhrhqm
mmiimx---

---ssnl*evyeqsignxhq*w**thspwsstilrtnh*rtke*kth*tlgfirl*ssry
frqic*tc*clk*dpprywfpihlyss*prskgsyrtip*iqkl*isyhgch*ylwpwyr
y*tcqcrhqlryg*ic

Frame C:
llaywiikkmysxny*r*gdvyiskkkr**y***s*fn**r*y*k***qyfixiiivik*
**s*x---

---RPICKKFMNNPLEXYINDGSKLTLHGLQQYYVPITEEQKNKKLIELLDSLDFNQAVI
FVKSVRRADALNKILQDIGFPSICIHRDLDQKDRIEQYRKFKNFESRIMVATNIFGRGID
IERVNVVINYDMAES
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA471 (CHA471Q) /CSM/CH/CHA4-C/CHA471Q.Seq.d/                       1082   0.0
VFL686 (VFL686Q) /CSM/VF/VFL6-D/VFL686Q.Seq.d/                        779   0.0
VFE359 (VFE359Q) /CSM/VF/VFE3-C/VFE359Q.Seq.d/                        779   0.0
VFA442 (VFA442Q) /CSM/VF/VFA4-B/VFA442Q.Seq.d/                        779   0.0
VFA314 (VFA314Q) /CSM/VF/VFA3-A/VFA314Q.Seq.d/                        779   0.0
SLH648 (SLH648Q) /CSM/SL/SLH6-B/SLH648Q.Seq.d/                        779   0.0
SFL133 (SFL133Q) /CSM/SF/SFL1-B/SFL133Q.Seq.d/                        779   0.0
SFL121 (SFL121Q) /CSM/SF/SFL1-A/SFL121Q.Seq.d/                        779   0.0
SFE227 (SFE227Q) /CSM/SF/SFE2-B/SFE227Q.Seq.d/                        779   0.0
SFD888 (SFD888Q) /CSM/SF/SFD8-D/SFD888Q.Seq.d/                        779   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CN208744|CN208744.1 Tor9242 Gametophyte rehydration Library Tortula ruralis cDNA, mRNAsequence.    82   1e-11  1
CD851895|CD851895.1 DH0ALL20ZA09ZZM1 HaDevR5 Helianthus annuus cDNA clone HaDevR520A09, mRNA sequence.    58   2e-04  1
CD145566|CD145566.1 MG1-0105U-A350-B05-U.B MG1-0105 Schistosoma mansoni cDNA clone MG1-0105U-A350-B05.B, mRNA sequence.    56   7e-04  1
CD116259|CD116259.1 ME1-0038P-A145-H11-U.B ME1-0038 Schistosoma mansoni cDNA clone ME1-0038P-A145-H11.B, mRNA sequence.    56   7e-04  1
CD163190|CD163190.1 ML1-0082U-V216-B10-U.B ML1-0082 Schistosoma mansoni cDNA clone ML1-0082U-V216-B10.B, mRNA sequence.    56   7e-04  1
CD183694|CD183694.1 MS1-0038U-A290-H01-U.B MS1-0038 Schistosoma mansoni cDNA clone MS1-0038U-A290-H01.B, mRNA sequence.    56   7e-04  1
BF936994|BF936994.1 EST460049 Schistosoma mansoni female, Phil LoVerde/Joe Merrick Schistosoma mansoni cDNA clone SMFCE26 5' end, mRNA sequence.    56   7e-04  1
CN207181|CN207181.1 Tor7599 Gametophyte rehydration Library Tortula ruralis cDNA, mRNA sequence.    54   0.003  1
AX488925|AX488925.1 Sequence 6225 from Patent WO02053728.          38   0.006  3
CD170044|CD170044.1 MS1-0002T-L205-C06-U.B MS1-0002 Schistosoma mansoni cDNA clone MS1-0002T-L205-C06.B, mRNA sequence.    52   0.011  1
dna update 2004. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O13792) RecName: Full=ATP-dependent RNA helicase uap56;        ...   186   4e-46
D89270_1(D89270|pid:none) Schizosaccharomyces pombe mRNA, partia...   186   4e-46
FM992691_22(FM992691|pid:none) Candida dubliniensis CD36 chromos...   181   1e-44
AM920437_2400(AM920437|pid:none) Penicillium chrysogenum Wiscons...   180   3e-44
(A1DL85) RecName: Full=ATP-dependent RNA helicase sub2;         ...   180   3e-44
(A1CMQ7) RecName: Full=ATP-dependent RNA helicase sub2;         ...   180   3e-44
AJ428513_1(AJ428513|pid:none) Chironomus tentans mRNA for HEL pr...   179   4e-44
(Q1DI07) RecName: Full=ATP-dependent RNA helicase SUB2;         ...   179   4e-44
(Q6BME5) RecName: Full=ATP-dependent RNA helicase SUB2;         ...   178   1e-43
CR940352_341(CR940352|pid:none) Theileria annulata strain Ankara...   177   1e-43
protein update 2009. 3.29
PSORT 

 psg: 0.63  gvh: 0.36  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for CHA471 is cyt
5' end seq. ID CHA471F
5' end seq. 
>CHA471F.Seq
CACTGTTGGCCTACTGGATTATTAAAAAAATGTACTCAGNAAATTATTGAAGATGAGGGG
ATGTATACATCTCCAAGAAAAAGAGATAATAATACTGATGATAAAGTTAGTTTAATTAAT
AAAGATAGTATTAGAAATAGTAATGACAGTATTTCATCGNCATCATCATCGTCATCAAAT
GATGATGATCATAATGNACNNNNNNNNNN
Length of 5' end seq. 209
3' end seq. ID CHA471Z
3' end seq. 
>CHA471Z.Seq
NNNNNNNNNNTTCGTCCAATCTGTAAGAAGTTTATGAACAATCCATTGGAAATNTACATC
AATGATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATACTACGTACCAATCACTGAA
GAACAAAAGAATAAAAAACTCATTGAACTCTTGGATTCATTAGACTTTAATCAAGCCGTT
ATTTTCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAAGATCCTCCAAGATATTGGT
TTCCCATCCATCTGTATTCATCGTGACCTCGATCAAAAGGATCGTATCGAACAATACCGT
AAATTCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAATATCTTTGGCCGTGGTATC
GATATTGAACGTGTCAATGTCGTCATCAACTACGATATGGCTGAATCTGC
Length of 3' end seq. 410
Connected seq. ID CHA471P
Connected seq. 
>CHA471P.Seq
CACTGTTGGCCTACTGGATTATTAAAAAAATGTACTCAGNAAATTATTGAAGATGAGGGG
ATGTATACATCTCCAAGAAAAAGAGATAATAATACTGATGATAAAGTTAGTTTAATTAAT
AAAGATAGTATTAGAAATAGTAATGACAGTATTTCATCGNCATCATCATCGTCATCAAAT
GATGATGATCATAATGNAC----------TTCGTCCAATCTGTAAGAAGTTTATGAACAA
TCCATTGGAAATNTACATCAATGATGGTAGTAAACTCACTCTCCATGGTCTTCAACAATA
CTACGTACCAATCACTGAAGAACAAAAGAATAAAAAACTCATTGAACTCTTGGATTCATT
AGACTTTAATCAAGCCGTTATTTTCGTCAAATCTGTTAGACGTGCTGATGCCTTAAATAA
GATCCTCCAAGATATTGGTTTCCCATCCATCTGTATTCATCGTGACCTCGATCAAAAGGA
TCGTATCGAACAATACCGTAAATTCAAAAACTTTGAATCTCGTATCATGGTTGCCACTAA
TATCTTTGGCCGTGGTATCGATATTGAACGTGTCAATGTCGTCATCAACTACGATATGGC
TGAATCTGC
Length of connected seq. 599
Full length Seq ID -
Full length Seq. -
Length of full length seq. -