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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHA328
Library CH
(Link to library)
Clone ID CHA328
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11582-1|Contig-U16358-1
Original site URL
Representative seq. ID CHA328P
(Link to Original site)
Representative DNA sequence 
>CHA328 (CHA328Q) /CSM/CH/CHA3-B/CHA328Q.Seq.d/
ACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACTACCTTTAACGAATATC
CATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCAATGGAAAAT
GGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAGTTATTGCAA
CTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGTTAGCAGCAA
AAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGATTAATTGGTC
AAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAATGGGTAAAA
TTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATTATGCAACTG
GTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATCACATTTTAA
TGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATTTCCCATGTG
CCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGGXXXXXXXXXXCTGGTAAATG
CATTGTCACCGATAATTGTAATGACAATAACATATGCAGTGACGATAGTTGCTCATTGGA
TGGCACATGTATTCACACTAAAAAGAATTGTGATGATGGAAATAAATGTACAAATGATTT
TTGTAAAGTTGATATTGGCTGCGTTCATAGTAATATCACTTGTGAATCAAGTTCAGTTTG
TATGGTTGCAAGTTGTGATTCGAATAAAGGTTGTATTGAATCACCAATAGTCTGTCCATC
AAGTGCATTTTGTTTAGTGGCTCAATGTGATGTAGGTTATGGTGGTTGTAATCAATATGA
TAAAGTTTGTATACCTGATGATCCTCATTGTCAATATGGTATTTGTGATAACTCAACTAA
AAAATGTACATTTAAAGATTTCGATCCACTTCCATTCAGATGTCAATCTGTGGCTGTAAA
AGCAGGTGTTATTGGTGGTGCTGCAATTGCTGGTGTAGTTGTTGGTGGTGCCGTTGCACT
TGGTTTAGCTTTATTTGGTGCTAAAGCNNGTTATAATCATTGGATGTCTTTAAAAATAAT
CAGATGGCAACCTCATCTGTAAATCCATTATANAACCATCACCACATCAAGGTACAAATC
CACTTNGGAAGCTCCTC
sequence update 2002.10.25
Translated Amino Acid sequence 
kkhflikqin*KMTTFNEYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTNNKVIAT
VRGAAPEEYETCIQAMLAAKVKWALTPAPRRGEIVRLIGQAMREKIEPLSKLISLEMGKI
YIEAKGEVQEFIDVCDYATGLSRSINGQVMPSERPNHILMETWNPLGLVGIITAFNFPCA
VLGWNAAISMIC---

---GKCIVTDNCNDNNICSDDSCSLDGTCIHTKKNCDDGNKCTNDFCKVDIGCVHSNITC
ESSSVCMVASCDSNKGCIESPIVCPSSAFCLVAQCDVGYGGCNQYDKVCIPDDPHCQYGI
CDNSTKKCTFKDFDPLPFRCQSVAVKAGVIGGAAIAGVVVGGAVALGLALFGAKAXYNHW
MSLKIIRWQPHL*ihyxtittsrykstxeap
Translated Amino Acid sequence (All Frames) 
Frame A:
tknif**nr*ikk*lpltnihswln*alklkimmessmengevlvkssnv*iqqiikllq
llevlhqknmkhvfkqc*qqk*ngh*hqlqeevklld*lvkp*ekklnhyqn*fh*kwvk
ftlklkvkyknsltyaimqlv*vdqsmvk*chlnvqitf*wkpgih*v*lvslllsishv
pylvgmlqsq*fa---

---lvnalspiivmtityavtivahwmahvftlkrivmmeinvqmifvklilaafivisl
vnqvqfvwlqvvirikvvlnhq*svhqvhfv*wlnvm*vmvvvinmikfvylmilivnmv
fvitqlknvhlkisihfhsdvnlwl*kqvllvvlqllv*llvvplhlv*lylvlkxviii
gcl*k*sdgnlicksiixpsphqgtnplxkll

Frame B:
qktfsnktdklkndyl*risilg*irh*s*k**wslqwkmgrcw*nhqmfksnk**sycn
c*rcctrri*nmyssnvsskskmgintsskkr*nc*inwssherkn*tiikinfirng*n
lh*s*r*strih*rmrlcnwfk*inqwssdai*tskshfngnlesirfswyhycfqfpmc
rtwleccnlndlr---

---w*mhchr*l**q*hmq*r*lligwhmysh*kel**wk*myk*fl*s*ywlrs**yhl
*ikfslygckl*fe*rly*itnslsikcilfsgsm*crlwwl*si**slyt**sslsiwy
l**ln*kmyi*rfrstsiqmsicgcksrcywwccncwcscwwcrctwfsfiwc*sxl*sl
dvfknnqmatssvnplxnhhhikvqihxgss

Frame C:
kkhflikqin*KMTTFNEYPFLAELGIKAENNDGVFNGKWGGAGEIIKCLNPTNNKVIAT
VRGAAPEEYETCIQAMLAAKVKWALTPAPRRGEIVRLIGQAMREKIEPLSKLISLEMGKI
YIEAKGEVQEFIDVCDYATGLSRSINGQVMPSERPNHILMETWNPLGLVGIITAFNFPCA
VLGWNAAISMIC---

---GKCIVTDNCNDNNICSDDSCSLDGTCIHTKKNCDDGNKCTNDFCKVDIGCVHSNITC
ESSSVCMVASCDSNKGCIESPIVCPSSAFCLVAQCDVGYGGCNQYDKVCIPDDPHCQYGI
CDNSTKKCTFKDFDPLPFRCQSVAVKAGVIGGAAIAGVVVGGAVALGLALFGAKAXYNHW
MSLKIIRWQPHL*ihyxtittsrykstxeap
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA328 (CHA328Q) /CSM/CH/CHA3-B/CHA328Q.Seq.d/                       2329   0.0
CFH643 (CFH643Q) /CSM/CF/CFH6-B/CFH643Q.Seq.d/                       1219   0.0
CFH274 (CFH274Q) /CSM/CF/CFH2-D/CFH274Q.Seq.d/                       1185   0.0
CFJ814 (CFJ814Q) /CSM/CF/CFJ8-A/CFJ814Q.Seq.d/                       1166   0.0
VFN595 (VFN595Q) /CSM/VF/VFN5-D/VFN595Q.Seq.d/                       1150   0.0
VFL522 (VFL522Q) /CSM/VF/VFL5-A/VFL522Q.Seq.d/                       1150   0.0
VFK658 (VFK658Q) /CSM/VF/VFK6-C/VFK658Q.Seq.d/                       1150   0.0
VFJ233 (VFJ233Q) /CSM/VF/VFJ2-B/VFJ233Q.Seq.d/                       1150   0.0
VFG476 (VFG476Q) /CSM/VF/VFG4-D/VFG476Q.Seq.d/                       1150   0.0
VFF640 (VFF640Q) /CSM/VF/VFF6-B/VFF640Q.Seq.d/                       1150   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117076|AC117076.3 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.   771   0.0    9
BU042811|BU042811.1 PP_LEa0014A23f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0014A23f, mRNA sequence.    74   9e-12  2
D88434|D88434.1 Malus domestica mRNA for protein abundantly expressed during apple fruit development, complete cds.    72   2e-08  1
CN883223|CN883223.1 010813AASB004877HT (AASB) Royal Gala 10 DAFB fruit Malus x domestica cDNA clone AASB004877, mRNA sequence.    72   2e-08  1
CN875989|CN875989.1 020813AARA008546HT (AARA) Royal Gala partially senescing leaf Malus x domestica cDNA clone AARA008546, mRNA sequence.    72   2e-08  1
CN903667|CN903667.1 021018ABCA005552HT (ABCA) Royal Gala fruit stored at 0.5 degrees C for 24 hours Malus x domestica cDNA clone ABCA005552, mRNA sequence.    72   2e-08  1
CV150833|CV150833.1 Mdst6010o01.y1 Mdst Malus x domestica cDNA clone Mdst6010o01 5' similar to TR:Q9ZPB7 Q9ZPB7 PROTEIN ABUNDANTLY EXPRESSED DURING APPLE FRUIT DEVELOPMENT. ;, mRNA sequence.    72   2e-08  1
CV149734|CV149734.1 Mdst6007g23.y1 Mdst Malus x domestica cDNA clone Mdst6007g23 5' similar to TR:Q9ZPB7 Q9ZPB7 PROTEIN ABUNDANTLY EXPRESSED DURING APPLE FRUIT DEVELOPMENT. ;, mRNA sequence.    72   2e-08  1
CN902319|CN902319.1 021014ABCA004075HT (ABCA) Royal Gala fruit stored at 0.5 degrees C for 24 hours Malus x domestica cDNA clone ABCA004075, mRNA sequence.    64   6e-06  1
CN895049|CN895049.1 010626AAYA004321HT (AAYA) Royal Gala 126 DAFB fruit cortex Malus x domestica cDNA clone AAYA004321, mRNA sequence.    64   6e-06  1
dna update 2004. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P83401) RecName: Full=Putative aldehyde dehydrogenase family 7 ...   374   e-102
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                283   6e-79
BC044367_1(BC044367|pid:none) Danio rerio aldehyde dehydrogenase...   232   2e-59
EF678715_1(EF678715|pid:none) Picea sitchensis clone WS0295_M17 ...   229   2e-58
BT044648_1(BT044648|pid:none) Salmo salar clone ssal-rgf-001-326...   226   9e-58
AF323586_1(AF323586|pid:none) Oryza sativa aldehyde dehydrogenas...   224   5e-57
AY883858_1(AY883858|pid:none) Sterkiella histriomuscorum turgor ...   221   4e-56
AY847462_1(AY847462|pid:none) Acanthopagrus schlegelii antiquiti...   221   5e-56
BT067707_1(BT067707|pid:none) Zea mays full-length cDNA clone ZM...   220   7e-56
AK297365_1(AK297365|pid:none) Homo sapiens cDNA FLJ56008 complet...   218   3e-55
protein update 2009. 3.29
PSORT 

 psg: 0.78  gvh: 0.31  alm: 0.22  top: 0.50  tms: 0.20  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: plasma membrane
	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: vesicles of secretory system

>> prediction for CHA328 is pla
5' end seq. ID CHA328F
5' end seq. 
>CHA328F.Seq
ACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACTACCTTTAACGAATATC
CATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCAATGGAAAAT
GGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAGTTATTGCAA
CTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGTTAGCAGCAA
AAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGATTAATTGGTC
AAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAATGGGTAAAA
TTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATTATGCAACTG
GTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATCACATTTTAA
TGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATTTCCCATGTG
CCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGGNNNNNNNNNN
Length of 5' end seq. 590
3' end seq. ID CHA328Z
3' end seq. 
>CHA328Z.Seq
NNNNNNNNNNCTGGTAAATGCATTGTCACCGATAATTGTAATGACAATAACATATGCAGT
GACGATAGTTGCTCATTGGATGGCACATGTATTCACACTAAAAAGAATTGTGATGATGGA
AATAAATGTACAAATGATTTTTGTAAAGTTGATATTGGCTGCGTTCATAGTAATATCACT
TGTGAATCAAGTTCAGTTTGTATGGTTGCAAGTTGTGATTCGAATAAAGGTTGTATTGAA
TCACCAATAGTCTGTCCATCAAGTGCATTTTGTTTAGTGGCTCAATGTGATGTAGGTTAT
GGTGGTTGTAATCAATATGATAAAGTTTGTATACCTGATGATCCTCATTGTCAATATGGT
ATTTGTGATAACTCAACTAAAAAATGTACATTTAAAGATTTCGATCCACTTCCATTCAGA
TGTCAATCTGTGGCTGTAAAAGCAGGTGTTATTGGTGGTGCTGCAATTGCTGGTGTAGTT
GTTGGTGGTGCCGTTGCACTTGGTTTAGCTTTATTTGGTGCTAAAGCNNGTTATAATCAT
TGGATGTCTTTAAAAATAATCAGATGGCAACCTCATCTGTAAATCCATTATANAACCATC
ACCACATCAAGGTACAAATCCACTTNGGAAGCTCCTC
Length of 3' end seq. 637
Connected seq. ID CHA328P
Connected seq. 
>CHA328P.Seq
ACAAAAAACATTTTCTAATAAAACAGATAAATTAAAAAATGACTACCTTTAACGAATATC
CATTCTTGGCTGAATTAGGCATTAAAGCTGAAAATAATGATGGAGTCTTCAATGGAAAAT
GGGGAGGTGCTGGTGAAATCATCAAATGTTTAAATCCAACAAATAATAAAGTTATTGCAA
CTGTTAGAGGTGCTGCACCAGAAGAATATGAAACATGTATTCAAGCAATGTTAGCAGCAA
AAGTAAAATGGGCATTAACACCAGCTCCAAGAAGAGGTGAAATTGTTAGATTAATTGGTC
AAGCCATGAGAGAAAAAATTGAACCATTATCAAAATTAATTTCATTAGAAATGGGTAAAA
TTTACATTGAAGCTAAAGGTGAAGTACAAGAATTCATTGACGTATGCGATTATGCAACTG
GTTTAAGTAGATCAATCAATGGTCAAGTGATGCCATCTGAACGTCCAAATCACATTTTAA
TGGAAACCTGGAATCCATTAGGTTTAGTTGGTATCATTACTGCTTTCAATTTCCCATGTG
CCGTACTTGGTTGGAATGCTGCAATCTCAATGATTTGCGG----------CTGGTAAATG
CATTGTCACCGATAATTGTAATGACAATAACATATGCAGTGACGATAGTTGCTCATTGGA
TGGCACATGTATTCACACTAAAAAGAATTGTGATGATGGAAATAAATGTACAAATGATTT
TTGTAAAGTTGATATTGGCTGCGTTCATAGTAATATCACTTGTGAATCAAGTTCAGTTTG
TATGGTTGCAAGTTGTGATTCGAATAAAGGTTGTATTGAATCACCAATAGTCTGTCCATC
AAGTGCATTTTGTTTAGTGGCTCAATGTGATGTAGGTTATGGTGGTTGTAATCAATATGA
TAAAGTTTGTATACCTGATGATCCTCATTGTCAATATGGTATTTGTGATAACTCAACTAA
AAAATGTACATTTAAAGATTTCGATCCACTTCCATTCAGATGTCAATCTGTGGCTGTAAA
AGCAGGTGTTATTGGTGGTGCTGCAATTGCTGGTGTAGTTGTTGGTGGTGCCGTTGCACT
TGGTTTAGCTTTATTTGGTGCTAAAGCNNGTTATAATCATTGGATGTCTTTAAAAATAAT
CAGATGGCAACCTCATCTGTAAATCCATTATANAACCATCACCACATCAAGGTACAAATC
CACTTNGGAAGCTCCTC
Length of connected seq. 1207
Full length Seq ID -
Full length Seq. -
Length of full length seq. -