>CHA261 (CHA261Q) /CSM/CH/CHA2-C/CHA261Q.Seq.d/
ATACATTACATTGACCAACATTTCCACTATGGTAACATTGAGACCATCAATGTTTGAACC
TCATTTATCAGAGTTTTTCATTCATTCATCTGATCCAGAGTATAGCATCAAATTGAAATT
AGAGATATTGACTCGTTTAGCAACACCTGAAAATATTGGTAAAATCTTGAAAGAGTTCAA
AGAGTATGTAAAGAATGAAGATAAGAAATTCGTTGCAGCCACAATTCAAGCGATTGGCTC
CTGCGCCTCTACAGTACCAGATGTAACCGAATCATGTATCTATGGTTTAATGTCCCTCCT
ATCCAATCAATCAACTGTTGTAGTTGCAGAGAGTGTAATTGTTTTAAAAAGATTATTACA
ATTGAATGCAACAAATGAAAAACTTGAGAAATTAGAAAAAGAAAAAGAAAAAGAAAAAGA
TGTCAAAGAAAATCAATCAACCATTTCAAAACATTCATCATCAAATAATTCAATTAAATA
TGATAATATAATTTTACATTTATCAAAACTATTAGATACACTTCAAGTACCAAGTGCACG
TGCATCAATCGTTTGGGTAATTGGTGAATATTGCTATCGTXXXXXXXXXXAGAATTATTA
TTGGGTCAACCGTTATTCCCTGGTGAGAATGGTATTGACCAATTGGTAGAGATTATAAAG
GTATTGGGTACACCAACCAAAGAACAAATTCATGCAATGAATCCATATTATACCTCATTT
AAATTCCCAGAGATCAAAGCAAACCCATGGCCAAGAGTATTCAAAGCTAAGGATGTACCG
GCCGAATCAATTGATTTAATCTCAAAAATCTTACTTTATGATCCATCCTCAAGATTAAAG
CCAGTTGAAATTTGTGCACATCCATTCTTTGATGAATTAAGAGATCCAAAGACATGTCTT
CCAGATGGTAAACCTTTACCACCCCTCTTCAACTTTACAATCGCAGAACAAACTTCAATC
GGTCCAAAACTAGCCAAAACATTAATTCCATCACATGCTATGAATCAAATTGAATTGCCA
TCACCTTTATTCCCAAATTTGGCAATCTCTTCTTCTAATCAATCCTCCTCTTCAAATTCA
AATGCAAATGTGTCATCAAATTTAAATTCTCATTCTGCTTCACCTTCAACNACCTCCTCC
TCTTCTTCTACTCCAAATTCAATTCCTGTTCAATCACCTTCTACAACCAACANTACTTCA
TCAACNACTNACAACACANCCACAACTACAACA

YITLTNISTMVTLRPSMFEPHLSEFFIHSSDPEYSIKLKLEILTRLATPENIGKILKEFK
EYVKNEDKKFVAATIQAIGSCASTVPDVTESCIYGLMSLLSNQSTVVVAESVIVLKRLLQ
LNATNEKLEKLEKEKEKEKDVKENQSTISKHSSSNNSIKYDNIILHLSKLLDTLQVPSAR
ASIVWVIGEYCYR---

---ELLLGQPLFPGENGIDQLVEIIKVLGTPTKEQIHAMNPYYTSFKFPEIKANPWPRVF
KAKDVPAESIDLISKILLYDPSSRLKPVEICAHPFFDELRDPKTCLPDGKPLPPLFNFTI
AEQTSIGPKLAKTLIPSHAMNQIELPSPLFPNLAISSSNQSSSSNSNANVSSNLNSHSAS
PSTTSSSSSTPNSIPVQSPSTTNXTSSTTXNTXTTTT

Frame A:
ihyidqhfhygnietinv*tsfirvfhsfi*srv*hqieirdidsfsnt*kyw*nlervq
rvcke*r*eircshnssdwllrlystrcnrimylwfnvppiqsinccscrecncfkkiit
iecnk*kt*eirkrkrkrkrcqrksinhfktfiik*fn*i**ynftfiktirytsstkct
cinrlgnw*ills---

---riiigstvipw*ewy*pigrdykgigytnqrtnscnesilyli*iprdqskpmaksi
qs*gctgrin*fnlknltl*silkikas*nlctsil**ikrskdmssrw*tfttplqlyn
rrtnfnrsktsqninsitcyesn*iaitfipkfgnlff*sillfkfkckcvikfkfsfcf
tfnxlllffyskfnscsitfynqxyfinxxqhxhnyn

Frame B:
YITLTNISTMVTLRPSMFEPHLSEFFIHSSDPEYSIKLKLEILTRLATPENIGKILKEFK
EYVKNEDKKFVAATIQAIGSCASTVPDVTESCIYGLMSLLSNQSTVVVAESVIVLKRLLQ
LNATNEKLEKLEKEKEKEKDVKENQSTISKHSSSNNSIKYDNIILHLSKLLDTLQVPSAR
ASIVWVIGEYCYR---

---ELLLGQPLFPGENGIDQLVEIIKVLGTPTKEQIHAMNPYYTSFKFPEIKANPWPRVF
KAKDVPAESIDLISKILLYDPSSRLKPVEICAHPFFDELRDPKTCLPDGKPLPPLFNFTI
AEQTSIGPKLAKTLIPSHAMNQIELPSPLFPNLAISSSNQSSSSNSNANVSSNLNSHSAS
PSTTSSSSSTPNSIPVQSPSTTNXTSSTTXNTXTTTT

Frame C:
tlh*ptfplw*h*dhqclnliyqsfsfihliqsiasn*n*ry*lv*qhlkilvks*kssk
sm*rmkirnslqpqfkrlapaplqyqm*pnhvsmv*cpsypinqll*lqrv*lf*kdyyn
*mqqmknlrn*kkkkkkkkmskkinqpfqnihhqiiqlnmii*fyiyqny*ihfkyqvhv
hqsfg*lvniai---

---nyywvnryslvrmvltnw*rl*rywvhqpknkfmq*ihiiphlnsqrskqthgqeys
klrmyrpnqli*sqksyfmihpqd*sqlkfvhihslmn*eiqrhvfqmvnlyhpsstlqs
qnklqsvqn*pkh*fhhml*iklnchhlysqiwqslllinpplqiqmqmchqi*ilillh
lqxpppllllqiqflfnhllqptxlhqxlttxpqlq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA261 (CHA261Q) /CSM/CH/CHA2-C/CHA261Q.Seq.d/                       1499   0.0
CFC364 (CFC364Q) /CSM/CF/CFC3-C/CFC364Q.Seq.d/                       1245   0.0
CFA794 (CFA794Q) /CSM/CF/CFA7-D/CFA794Q.Seq.d/                       1245   0.0
CFG633 (CFG633Q) /CSM/CF/CFG6-B/CFG633Q.Seq.d/                       1229   0.0
AFH493 (AFH493Q) /CSM/AF/AFH4-D/AFH493Q.Seq.d/                       1203   0.0
SSL104 (SSL104Q) /CSM/SS/SSL1-A/SSL104Q.Seq.d/                       1193   0.0
CHB117 (CHB117Q) /CSM/CH/CHB1-A/CHB117Q.Seq.d/                       1178   0.0
CFC263 (CFC263Q) /CSM/CF/CFC2-C/CFC263Q.Seq.d/                       1174   0.0
SFD633 (SFD633Q) /CSM/SF/SFD6-B/SFD633Q.Seq.d/                       1045   0.0
AFH106 (AFH106Q) /CSM/AF/AFH1-A/AFH106Q.Seq.d/                        946   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L34674|L34674.2 Dictyostelium discoideum glycogen synthase kinase 3 mRNA, complete cds.  1245   0.0    1
AC116920|AC116920.3 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.  1245   0.0    1
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   735   0.0    21
BJ552082|BJ552082.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah47d12, 3' mRNA sequence.    44   3e-07  3
BJ552583|BJ552583.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah55o11, 3' mRNA sequence.    44   3e-07  3
AV927751|AV927751.1 Hordeum vulgare subsp. vulgare cv.Haruna Nijo cDNA clone:basd3i06, 3' mRNA sequence.    44   4e-07  3
BD365141|BD365141.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   4e-07  3
BD365143|BD365143.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   4e-07  3
BD365137|BD365137.1 Newly found single nucleotide polymorphisms (SNPs) on genes and use thereof.    44   4e-07  3
BJ552379|BJ552379.1 Hordeum vulgare subsp. spontaneum strain H602 cDNA clone:bah56e23, 3' mRNA sequence.    44   4e-07  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A55476(A55476)protein kinase (EC 2.7.1.37) gskA - slime mold  (D...   314   4e-84
(Q556J8) RecName: Full=AP-3 complex subunit beta; AltName: Full=...   312   2e-83
EF677494_1(EF677494|pid:none) Picea sitchensis clone WS02769_M19...   189   2e-46
AC096856_19(AC096856|pid:none) Oryza sativa chromosome 3 BAC OSJ...   186   1e-45
EF085206_1(EF085206|pid:none) Picea sitchensis clone WS02728_K01...   186   1e-45
T03601(T03601) shaggy protein kinase (EC 2.7.1.-) 6 - common tob...   184   4e-45
AJ224163_1(AJ224163|pid:none) Nicotiana tabacum mRNA for shaggy-...   184   4e-45
AY339069_1(AY339069|pid:none) Physcomitrella patens shaggy-relat...   183   1e-44
AY339070_1(AY339070|pid:none) Physcomitrella patens shaggy-relat...   182   3e-44
AB032265_1(AB032265|pid:none) Danio rerio mRNA for glycogen synt...   181   3e-44

 psg: 0.86  gvh: 0.39  alm: 0.37  top: 0.47  tms: 0.00  mit: 0.37  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CHA261 is cyt

>CHA261F.Seq
ATACATTACATTGACCAACATTTCCACTATGGTAACATTGAGACCATCAATGTTTGAACC
TCATTTATCAGAGTTTTTCATTCATTCATCTGATCCAGAGTATAGCATCAAATTGAAATT
AGAGATATTGACTCGTTTAGCAACACCTGAAAATATTGGTAAAATCTTGAAAGAGTTCAA
AGAGTATGTAAAGAATGAAGATAAGAAATTCGTTGCAGCCACAATTCAAGCGATTGGCTC
CTGCGCCTCTACAGTACCAGATGTAACCGAATCATGTATCTATGGTTTAATGTCCCTCCT
ATCCAATCAATCAACTGTTGTAGTTGCAGAGAGTGTAATTGTTTTAAAAAGATTATTACA
ATTGAATGCAACAAATGAAAAACTTGAGAAATTAGAAAAAGAAAAAGAAAAAGAAAAAGA
TGTCAAAGAAAATCAATCAACCATTTCAAAACATTCATCATCAAATAATTCAATTAAATA
TGATAATATAATTTTACATTTATCAAAACTATTAGATACACTTCAAGTACCAAGTGCACG
TGCATCAATCGTTTGGGTAATTGGTGAATATTGCTATCGTNNNNNNNNNN

>CHA261Z.Seq
NNNNNNNNNNAGAATTATTATTGGGTCAACCGTTATTCCCTGGTGAGAATGGTATTGACC
AATTGGTAGAGATTATAAAGGTATTGGGTACACCAACCAAAGAACAAATTCATGCAATGA
ATCCATATTATACCTCATTTAAATTCCCAGAGATCAAAGCAAACCCATGGCCAAGAGTAT
TCAAAGCTAAGGATGTACCGGCCGAATCAATTGATTTAATCTCAAAAATCTTACTTTATG
ATCCATCCTCAAGATTAAAGCCAGTTGAAATTTGTGCACATCCATTCTTTGATGAATTAA
GAGATCCAAAGACATGTCTTCCAGATGGTAAACCTTTACCACCCCTCTTCAACTTTACAA
TCGCAGAACAAACTTCAATCGGTCCAAAACTAGCCAAAACATTAATTCCATCACATGCTA
TGAATCAAATTGAATTGCCATCACCTTTATTCCCAAATTTGGCAATCTCTTCTTCTAATC
AATCCTCCTCTTCAAATTCAAATGCAAATGTGTCATCAAATTTAAATTCTCATTCTGCTT
CACCTTCAACNACCTCCTCCTCTTCTTCTACTCCAAATTCAATTCCTGTTCAATCACCTT
CTACAACCAACANTACTTCATCAACNACTNACAACACANCCACAACTACAACA

>CHA261P.Seq
ATACATTACATTGACCAACATTTCCACTATGGTAACATTGAGACCATCAATGTTTGAACC
TCATTTATCAGAGTTTTTCATTCATTCATCTGATCCAGAGTATAGCATCAAATTGAAATT
AGAGATATTGACTCGTTTAGCAACACCTGAAAATATTGGTAAAATCTTGAAAGAGTTCAA
AGAGTATGTAAAGAATGAAGATAAGAAATTCGTTGCAGCCACAATTCAAGCGATTGGCTC
CTGCGCCTCTACAGTACCAGATGTAACCGAATCATGTATCTATGGTTTAATGTCCCTCCT
ATCCAATCAATCAACTGTTGTAGTTGCAGAGAGTGTAATTGTTTTAAAAAGATTATTACA
ATTGAATGCAACAAATGAAAAACTTGAGAAATTAGAAAAAGAAAAAGAAAAAGAAAAAGA
TGTCAAAGAAAATCAATCAACCATTTCAAAACATTCATCATCAAATAATTCAATTAAATA
TGATAATATAATTTTACATTTATCAAAACTATTAGATACACTTCAAGTACCAAGTGCACG
TGCATCAATCGTTTGGGTAATTGGTGAATATTGCTATCGT----------AGAATTATTA
TTGGGTCAACCGTTATTCCCTGGTGAGAATGGTATTGACCAATTGGTAGAGATTATAAAG
GTATTGGGTACACCAACCAAAGAACAAATTCATGCAATGAATCCATATTATACCTCATTT
AAATTCCCAGAGATCAAAGCAAACCCATGGCCAAGAGTATTCAAAGCTAAGGATGTACCG
GCCGAATCAATTGATTTAATCTCAAAAATCTTACTTTATGATCCATCCTCAAGATTAAAG
CCAGTTGAAATTTGTGCACATCCATTCTTTGATGAATTAAGAGATCCAAAGACATGTCTT
CCAGATGGTAAACCTTTACCACCCCTCTTCAACTTTACAATCGCAGAACAAACTTCAATC
GGTCCAAAACTAGCCAAAACATTAATTCCATCACATGCTATGAATCAAATTGAATTGCCA
TCACCTTTATTCCCAAATTTGGCAATCTCTTCTTCTAATCAATCCTCCTCTTCAAATTCA
AATGCAAATGTGTCATCAAATTTAAATTCTCATTCTGCTTCACCTTCAACNACCTCCTCC
TCTTCTTCTACTCCAAATTCAATTCCTGTTCAATCACCTTCTACAACCAACANTACTTCA
TCAACNACTNACAACACANCCACAACTACAACA