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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHA247
Library CH
(Link to library)
Clone ID CHA247
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID CHA247P
(Link to Original site)
Representative DNA sequence 
>CHA247 (CHA247Q) /CSM/CH/CHA2-B/CHA247Q.Seq.d/
GGAAAAACAAAACCATTAAAATGAATACAATTTATAAAACTCTTTGCTCACCAAAAAAAT
TTATAATTGGAAAGAATTTATTAAATGATCCTTCAAAATTTATTAAAGATTTTGGAAATA
AATGTTTTATTGTTTGTGATGAGAGATTAATTGATCAAGTAAATCAAAAAACTGTAAAGT
CTTTAGAAAAAGAGAATATTATATCTCACGTTGAAAAATTTAAAATTGAATGTACAAAAA
ATGAAGTTGAAAGAATGTGTTCAATTAAAAAATCTAAAGGTTCAAATGTAATTTTAGGAA
TTGGTGGTGGTAAAACAATTGATACTGCAAAAGCAATGGCATTTTATGAAAAATGTCCAG
TAGTAATATTTCCAACAGCAGCATCAACTGATGCACCATGTACATCATTAGCAGTTTTAT
ACAAAGATAATGGAGAATTTGATCAATATCTTTATATTCCAAATAATCCAGATGTTGTTT
TAGTTGATCCAGTTGTTATGGTTACTGCACCACCAAGATTATTTTCTTCAGGAGTAGGTG
ATGCACTTGCAACTTATTTTGAAGCAAGACAATGTTTCCAAAGTTTTGGAAATAATTTAA
CCAATACAAAACCATCATTGATTXXXXXXXXXXCTGGTATCATTAAACCAGGTATGGTCG
TCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAAC
AACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCA
AAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTG
AAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACT
CACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATA
AAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAA
AGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCT
TCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCG
TCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGTGC
TGCGCCCCATCAAAAAAGAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
KNKTIKMNTIYKTLCSPKKFIIGKNLLNDPSKFIKDFGNKCFIVCDERLIDQVNQKTVKS
LEKENIISHVEKFKIECTKNEVERMCSIKKSKGSNVILGIGGGKTIDTAKAMAFYEKCPV
VIFPTAASTDAPCTSLAVLYKDNGEFDQYLYIPNNPDVVLVDPVVMVTAPPRLFSSGVGD
ALATYFEARQCFQSFGNNLTNTKPSLI---

---GIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVA
GDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAV
VAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVK
KAPGKAGIRKVLRPIKKE
Translated Amino Acid sequence (All Frames) 
Frame A:
gktkplk*iqfiklfahqknl*leriy*milqnllkileinvllfvmrd*lik*ikkl*s
l*kkrilyltlknlklnvqkmklkecvqlknlkvqm*f*elvvvkqlilqkqwhfmknvq
**yfqqqhqlmhhvhh*qfytkimenlinififqiiqmlf*liqllwllhhqdyflqe*v
mhlqlilkqdnvskvleii*piqnhh*---

---lvslnqvwssplpqlvsqlksnqskcitnnsqkpvqvtm*vstlktfqskklkevws
lvtpktihhkklknsllklssstiqvksmlvthqs*ivtlltlpvnslkssiklivvlvp
slpkkvlppss*rmvmplwsn*phlvqcvlnpslnthh*vvspsei*dkpspsvssnqps
rkpqvkpv*erccapskkk

Frame B:
ekqnh*neynl*nslltkkiynwkefik*sfkiy*rfwk*mfycl**ein*ssksknckv
frkreyyisr*ki*n*mykk*s*knvfn*ki*rfkcnfrnwww*nn*ycksngil*kmss
snisnssin*ctmyiissfiqr*wri*sislysk*srccfs*sscygycttkiiffrsr*
ctcnlf*sktmfpkfwk*fnqyktiid---

---wyh*trygrhlcpswsln*sqisrnasrttprspsr*qcrfqr*krfsqrn*krygr
w*lqkrsttrn*kircssyrpqpsrsnpcwlltslrlshcshcl*ih*nrr*s*ssywcr
rcqrrycrrrlkew*crygrinpissnvc*ilh*iptirsfrrqryetnrrrrchqinrq
espr*srykkgaaphqkr

Frame C:
KNKTIKMNTIYKTLCSPKKFIIGKNLLNDPSKFIKDFGNKCFIVCDERLIDQVNQKTVKS
LEKENIISHVEKFKIECTKNEVERMCSIKKSKGSNVILGIGGGKTIDTAKAMAFYEKCPV
VIFPTAASTDAPCTSLAVLYKDNGEFDQYLYIPNNPDVVLVDPVVMVTAPPRLFSSGVGD
ALATYFEARQCFQSFGNNLTNTKPSLI---

---GIIKPGMVVTFAPAGLSTEVKSVEMHHEQLPEARPGDNVGFNVKNVSVKEIKRGMVA
GDSKNDPPQETEKFVAQVIVLNHPGQIHAGYSPVLDCHTAHIACKFTEIVDKVDRRTGAV
VAKEGTAAVVLKNGDAAMVELTPSRPMCVESFTEYPPLGRFAVRDMRQTVAVGVIKSTVK
KAPGKAGIRKVLRPIKKE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA247 (CHA247Q) /CSM/CH/CHA2-B/CHA247Q.Seq.d/                       2319   0.0
CFF833 (CFF833Q) /CSM/CF/CFF8-B/CFF833Q.Seq.d/                       1176   0.0
VFO774 (VFO774Q) /CSM/VF/VFO7-D/VFO774Q.Seq.d/                       1166   0.0
VFO757 (VFO757Q) /CSM/VF/VFO7-C/VFO757Q.Seq.d/                       1166   0.0
VFB219 (VFB219Q) /CSM/VF/VFB2-A/VFB219Q.Seq.d/                       1166   0.0
VFA633 (VFA633Q) /CSM/VF/VFA6-B/VFA633Q.Seq.d/                       1166   0.0
VFA160 (VFA160Q) /CSM/VF/VFA1-C/VFA160Q.Seq.d/                       1166   0.0
AFJ556 (AFJ556Q) /CSM/AF/AFJ5-C/AFJ556Q.Seq.d/                       1166   0.0
CFK417 (CFK417Q) /CSM/CF/CFK4-A/CFK417Q.Seq.d/                       1162   0.0
VFA634 (VFA634Q) /CSM/VF/VFA6-B/VFA634Q.Seq.d/                       1160   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X55973|X55973.1 D. discoideum EF1-I gene for elongation factor 1 alpha.  1098   0.0    2
X55972|X55972.1 D. discoideum EF1-II gene for elongation factor 1 alpha.  1051   0.0    2
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.   585   0.0    10
AF016242|AF016242.1 Dictyostelium discoideum protein synthesis elongation factor 1-alpha (tef2) gene, partial cds.   922   0.0    1
M29935|M29935.1 C.albicans elongation factor 1-alpha (TEF-2) gene, complete cds.   117   1e-81  5
M29934|M29934.1 C.albicans elongation factor 1-alpha (TEF-1) gene, complete cds.   117   2e-80  6
AX109679|AX109679.1 Sequence 412 from Patent WO0123604.           101   1e-73  5
AX109682|AX109682.1 Sequence 415 from Patent WO0123604.            98   5e-73  5
AX109681|AX109681.1 Sequence 414 from Patent WO0123604.            98   5e-73  5
AX109714|AX109714.1 Sequence 447 from Patent WO0123604.           121   4e-70  5
dna update 2004. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116330_35(AC116330|pid:none) Dictyostelium discoideum chromoso...   374   e-102
X55972_1(X55972|pid:none) D. discoideum EF1-II gene for elongati...   372   e-101
(P18624) RecName: Full=Elongation factor 1-alpha;          Short...   372   e-101
X55973_1(X55973|pid:none) D. discoideum EF1-I gene for elongatio...   371   e-101
FJ539138_1(FJ539138|pid:none) Ignatius tetrasporus strain UTEX B...   281   2e-74
(P41745) RecName: Full=Elongation factor 1-alpha;          Short...   279   1e-73
AY542532_1(AY542532|pid:none) Cladonema radiatum translation elo...   277   6e-73
AJ581004_1(AJ581004|pid:none) Axinella verrucosa mRNA for elonga...   277   6e-73
Y09023_1(Y09023|pid:none) G.cydonium mRNA for elongation factor ...   276   1e-72
AY445082_1(AY445082|pid:none) Metarhizium anisopliae strain E6 t...   275   2e-72
protein update 2009. 3.29
PSORT 

 psg: 0.73  gvh: 0.37  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for CHA247 is cyt
5' end seq. ID CHA247F
5' end seq. 
>CHA247F.Seq
GGAAAAACAAAACCATTAAAATGAATACAATTTATAAAACTCTTTGCTCACCAAAAAAAT
TTATAATTGGAAAGAATTTATTAAATGATCCTTCAAAATTTATTAAAGATTTTGGAAATA
AATGTTTTATTGTTTGTGATGAGAGATTAATTGATCAAGTAAATCAAAAAACTGTAAAGT
CTTTAGAAAAAGAGAATATTATATCTCACGTTGAAAAATTTAAAATTGAATGTACAAAAA
ATGAAGTTGAAAGAATGTGTTCAATTAAAAAATCTAAAGGTTCAAATGTAATTTTAGGAA
TTGGTGGTGGTAAAACAATTGATACTGCAAAAGCAATGGCATTTTATGAAAAATGTCCAG
TAGTAATATTTCCAACAGCAGCATCAACTGATGCACCATGTACATCATTAGCAGTTTTAT
ACAAAGATAATGGAGAATTTGATCAATATCTTTATATTCCAAATAATCCAGATGTTGTTT
TAGTTGATCCAGTTGTTATGGTTACTGCACCACCAAGATTATTTTCTTCAGGAGTAGGTG
ATGCACTTGCAACTTATTTTGAAGCAAGACAATGTTTCCAAAGTTTTGGAAATAATTTAA
CCAATACAAAACCATCATTGATTNNNNNNNNNN
Length of 5' end seq. 633
3' end seq. ID CHA247Z
3' end seq. 
>CHA247Z.Seq
NNNNNNNNNNCTGGTATCATTAAACCAGGTATGGTCGTCACCTTTGCCCCAGCTGGTCTC
TCAACTGAAGTCAAATCAGTCGAAATGCATCACGAACAACTCCCAGAAGCCCGTCCAGGT
GACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCAAAGAAATTAAAAGAGGTATGGTC
GCTGGTGACTCCAAAAACGATCCACCACAAGAAACTGAAAAATTCGTTGCTCAAGTTATC
GTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACTCACCAGTCTTAGATTGTCACACT
GCTCACATTGCCTGTAAATTCACTGAAATCGTCGATAAAGTTGATCGTCGTACTGGTGCC
GTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAAAGAATGGTGATGCCGCTATGGTC
GAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCTTCACTGAATACCCACCATTAGGT
CGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCGTCGGTGTCATCAAATCAACCGTC
AAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGTGCTGCGCCCCATCAAAAAAGAAA
Length of 3' end seq. 598
Connected seq. ID CHA247P
Connected seq. 
>CHA247P.Seq
GGAAAAACAAAACCATTAAAATGAATACAATTTATAAAACTCTTTGCTCACCAAAAAAAT
TTATAATTGGAAAGAATTTATTAAATGATCCTTCAAAATTTATTAAAGATTTTGGAAATA
AATGTTTTATTGTTTGTGATGAGAGATTAATTGATCAAGTAAATCAAAAAACTGTAAAGT
CTTTAGAAAAAGAGAATATTATATCTCACGTTGAAAAATTTAAAATTGAATGTACAAAAA
ATGAAGTTGAAAGAATGTGTTCAATTAAAAAATCTAAAGGTTCAAATGTAATTTTAGGAA
TTGGTGGTGGTAAAACAATTGATACTGCAAAAGCAATGGCATTTTATGAAAAATGTCCAG
TAGTAATATTTCCAACAGCAGCATCAACTGATGCACCATGTACATCATTAGCAGTTTTAT
ACAAAGATAATGGAGAATTTGATCAATATCTTTATATTCCAAATAATCCAGATGTTGTTT
TAGTTGATCCAGTTGTTATGGTTACTGCACCACCAAGATTATTTTCTTCAGGAGTAGGTG
ATGCACTTGCAACTTATTTTGAAGCAAGACAATGTTTCCAAAGTTTTGGAAATAATTTAA
CCAATACAAAACCATCATTGATT----------CTGGTATCATTAAACCAGGTATGGTCG
TCACCTTTGCCCCAGCTGGTCTCTCAACTGAAGTCAAATCAGTCGAAATGCATCACGAAC
AACTCCCAGAAGCCCGTCCAGGTGACAATGTAGGTTTCAACGTTAAAAACGTTTCAGTCA
AAGAAATTAAAAGAGGTATGGTCGCTGGTGACTCCAAAAACGATCCACCACAAGAAACTG
AAAAATTCGTTGCTCAAGTTATCGTCCTCAACCATCCAGGTCAAATCCATGCTGGTTACT
CACCAGTCTTAGATTGTCACACTGCTCACATTGCCTGTAAATTCACTGAAATCGTCGATA
AAGTTGATCGTCGTACTGGTGCCGTCGTTGCCAAAGAAGGTACTGCCGCCGTCGTCTTAA
AGAATGGTGATGCCGCTATGGTCGAATTAACCCCATCTCGTCCAATGTGTGTTGAATCCT
TCACTGAATACCCACCATTAGGTCGTTTCGCCGTCAGAGATATGAGACAAACCGTCGCCG
TCGGTGTCATCAAATCAACCGTCAAGAAAGCCCCAGGTAAAGCCGGTATAAGAAAGGTGC
TGCGCCCCATCAAAAAAGAAA
Length of connected seq. 1211
Full length Seq ID -
Full length Seq. -
Length of full length seq. -