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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF248
Library AF
(Link to library)
Clone ID AFF248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13845-1
Original site URL
Representative seq. ID AFF248P
(Link to Original site)
Representative DNA sequence 
>AFF248 (AFF248Q) /CSM/AF/AFF2-B/AFF248Q.Seq.d/
AATTTTTATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTAC
ACGCACGCACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATA
CAGCAAACGCAAATTATTGTATCTTTCAAATTAAAAAAAAAAXXXXXXXXXXCTACAACA
GCAAAACCNNCNAATAATAATAATAATAATAATGATGATGAGGATGAAGATGAAGATGGT
GATATTCCAGATTTAGATGATTTCCAAGATGATAATATAATTGAAGAAGAGGATCCAGCT
GTATTATCAAAAAATAATAAAACAACAACTACTACAACAGCTAACAACAATAATAATAAT
NATTCAGAAAATAAAGTTGAAGATAATGATAATATTTTACGNACTCGNACATATGATATT
TCAATCACTTATGATAAATATTATCAAACNCCAAGAGTTTGGTTATTTGGATATGATGAA
AATAGAAAACCATTGAAACCAGAAGAAATCTTTGAAGATATTTCAGAAGATCATGCACAT
AAAACTGTCACAATCGATAGCCACCCACATTTAGGTATCTCATTTGCATACATTCATCCA
TGTAGACATGCT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
flxhtqkhf*iii*tthilhartir*ni*ly*YTIKKHYTANANYCIFQIKKK---

---TTAKPXNNNNNNNDDEDEDEDGDIPDLDDFQDDNIIEEEDPAVLSKNNKTTTTTTAN
NNNNNXSENKVEDNDNILRTRTYDISITYDKYYQTPRVWLFGYDENRKPLKPEEIFEDIS
EDHAHKTVTIDSHPHLGISFAYIHPCRHA
Translated Amino Acid sequence (All Frames) 
Frame A:
nfyxthknifr*lfrphtfythaqyvriynyinir*kniiqqtqiivsfklkkk---

---lqqqnxxiiiiiimmmrmkmkmvifqi*miskmii*lkkriqlyyqkiikqqllqql
ttiiixiqkiklkimiifyxlxhmifqslminiikxqefgyldmmkienh*nqkkslkif
qkimhiklsqsiathi*vshlhtfihvdm

Frame B:
ifxthtktfldnyldhthftrthntleyiiiliydkktlyskrkllylsn*kk---

---ynsktxx*********g*r*rw*ysrfr*fpr**yn*rrgssciikk**nnnyyns*
qq***xfrk*s*r***yftxsxi*yfnhl**ilsnxkslviwi**k*ktietrrnl*ryf
rrsct*nchnr*pptfrylicihssm*tc

Frame C:
flxhtqkhf*iii*tthilhartir*ni*ly*YTIKKHYTANANYCIFQIKKK---

---TTAKPXNNNNNNNDDEDEDEDGDIPDLDDFQDDNIIEEEDPAVLSKNNKTTTTTTAN
NNNNNXSENKVEDNDNILRTRTYDISITYDKYYQTPRVWLFGYDENRKPLKPEEIFEDIS
EDHAHKTVTIDSHPHLGISFAYIHPCRHA
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFI445 (VFI445Q) /CSM/VF/VFI4-B/VFI445Q.Seq.d/                        648   0.0
SLB748 (SLB748Q) /CSM/SL/SLB7-B/SLB748Q.Seq.d/                        648   0.0
SFC839 (SFC839Q) /CSM/SF/SFC8-B/SFC839Q.Seq.d/                        648   0.0
CFB657 (CFB657Q) /CSM/CF/CFB6-C/CFB657Q.Seq.d/                        648   0.0
AFO525 (AFO525Q) /CSM/AF/AFO5-B/AFO525Q.Seq.d/                        648   0.0
AFM817 (AFM817Q) /CSM/AF/AFM8-A/AFM817Q.Seq.d/                        648   0.0
AFF248 (AFF248Q) /CSM/AF/AFF2-B/AFF248Q.Seq.d/                        648   0.0
SFI461 (SFI461Q) /CSM/SF/SFI4-C/SFI461Q.Seq.d/                        642   0.0
CFH171 (CFH171Q) /CSM/CF/CFH1-C/CFH171Q.Seq.d/                        642   0.0
AFO275 (AFO275Q) /CSM/AF/AFO2-D/AFO275Q.Seq.d/                        642   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            44   3e-11  11
BU582697|BU582697.1 rc67g08.y2 Meloidogyne hapla egg SL1 TOPO v1 Meloidogyne hapla cDNA similar to WP:CE25486 Y55F3AM.4 ;, mRNA sequence.    60   6e-10  2
AE014824|AE014824.1 Plasmodium falciparum 3D7 chromosome 14 section 9 of 13 of the complete sequence.    38   1e-09  11
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           40   6e-09  13
AE014825|AE014825.1 Plasmodium falciparum 3D7 chromosome 14 section 10 of 13 of the complete sequence.    42   2e-08  14
AE014828|AE014828.1 Plasmodium falciparum 3D7 chromosome 14 section 13 of 13 of the complete sequence.    36   4e-08  12
CR382398|CR382398.1 Plasmodium falciparum chromosome 6, complete sequence; segment 1/5.    38   9e-08  13
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    42   1e-07  14
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   1e-07  14
AE014821|AE014821.1 Plasmodium falciparum 3D7 chromosome 14 section 6 of 13 of the complete sequence.    40   2e-07  14
dna update 2004. 5. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FJ747649_1(FJ747649|pid:none) Triticum aestivum ATG3 protein (AT...   129   5e-29
AY350711_16(AY350711|pid:none) Sisymbrium irio clone BAC Sio11E1...   126   4e-28
BT052164_1(BT052164|pid:none) Medicago truncatula clone MTYF9_FA...   126   4e-28
(Q0WWQ1) RecName: Full=Autophagy-related protein 3; AltName: Ful...   125   9e-28
EU972874_1(EU972874|pid:none) Zea mays clone 389483 autophagy-re...   124   3e-27
FJ416327_1(FJ416327|pid:none) Bombyx mori autophagy related prot...   123   4e-27
AM910989_29(AM910989|pid:none) Plasmodium knowlesi strain H chro...   122   8e-27
BT057047_1(BT057047|pid:none) Salmo salar clone ssal-sjb-020-072...   115   7e-25
(Q6GQE7) RecName: Full=Autophagy-related protein 3; AltName: Ful...   114   2e-24
(Q0VCL3) RecName: Full=Autophagy-related protein 3;  &BC120110_1...   114   3e-24
protein update 2009. 4.19
PSORT 

 psg: 0.79  gvh: 0.21  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for AFF248 is nuc
5' end seq. ID AFF248F
5' end seq. 
>AFF248F.Seq
AATTTTTATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTAC
ACGCACGCACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATA
CAGCAAACGCAAATTATTGTATCTTTCAAATTAAAAAAAAAA----------
Length of 5' end seq. 162
3' end seq. ID AFF248Z
3' end seq. 
>AFF248Z.Seq
----------CTACAACAGCAAAACCNNCNAATAATAATAATAATAATAATGATGATGAG
GATGAAGATGAAGATGGTGATATTCCAGATTTAGATGATTTCCAAGATGATAATATAATT
GAAGAAGAGGATCCAGCTGTATTATCAAAAAATAATAAAACAACAACTACTACAACAGCT
AACAACAATAATAATAATNATTCAGAAAATAAAGTTGAAGATAATGATAATATTTTACGN
ACTCGNACATATGATATTTCAATCACTTATGATAAATATTATCAAACNCCAAGAGTTTGG
TTATTTGGATATGATGAAAATAGAAAACCATTGAAACCAGAAGAAATCTTTGAAGATATT
TCAGAAGATCATGCACATAAAACTGTCACAATCGATAGCCACCCACATTTAGGTATCTCA
TTTGCATACATTCATCCATGTAGACATGCT
Length of 3' end seq. 440
Connected seq. ID AFF248P
Connected seq. 
>AFF248P.Seq
AATTTTTATNACACACACAAAAACATTTTTAGATAATTATTTAGACCACACACATTTTAC
ACGCACGCACAATACGTTAGAATATATAATTATATTAATATACGATAAAAAAACATTATA
CAGCAAACGCAAATTATTGTATCTTTCAAATTAAAAAAAAAA----------CTACAACA
GCAAAACCNNCNAATAATAATAATAATAATAATGATGATGAGGATGAAGATGAAGATGGT
GATATTCCAGATTTAGATGATTTCCAAGATGATAATATAATTGAAGAAGAGGATCCAGCT
GTATTATCAAAAAATAATAAAACAACAACTACTACAACAGCTAACAACAATAATAATAAT
NATTCAGAAAATAAAGTTGAAGATAATGATAATATTTTACGNACTCGNACATATGATATT
TCAATCACTTATGATAAATATTATCAAACNCCAAGAGTTTGGTTATTTGGATATGATGAA
AATAGAAAACCATTGAAACCAGAAGAAATCTTTGAAGATATTTCAGAAGATCATGCACAT
AAAACTGTCACAATCGATAGCCACCCACATTTAGGTATCTCATTTGCATACATTCATCCA
TGTAGACATGCT
Length of connected seq. 602
Full length Seq ID -
Full length Seq. -
Length of full length seq. -