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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CHA214
Library CH
(Link to library)
Clone ID CHA214
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12898-1|Contig-U16511-1
Original site URL
Representative seq. ID CHA214P
(Link to Original site)
Representative DNA sequence 
>CHA214 (CHA214Q) /CSM/CH/CHA2-A/CHA214Q.Seq.d/
CTGTTGGCCTACTGGAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTA
GAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGAC
AAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAA
AAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTA
AATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATA
GTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAG
ATGTTTATGGAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTG
TTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATG
TTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAAT
CACCATACACAAGTTTATCACATTTCCTCXXXXXXXXXXAACAATTTCAGATTCAACCGT
AACTCTGTTCAACGTAATGCCAAACCAGCTCAACAACAAAGATCTGCTCAACATGCCAAA
CCAGCTCAACATGGTAAACCAGCTCAACATGCCACTCAACAACAACATGCCAAACCAGCC
CAAAATGCTAAACCAGTTCAACATAACGCCCAACAACAACATGCCAAACCAGCTCAACAT
GCCGCTCAACAACAACATGCCAAACCAGCTCAACATGTCGCTCAACAACAACAACAAGAA
CACCAAAGACAAGAAGCTCTCGCAAAACAACAAGCTCGTATTAGACAACAAAATCTTGTC
AAACAAGCTGCCCAAAAGAAACNNNCCTCTCAACGTGCTGCCTCTAAAAATCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
VGLLEQVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAK
NKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKD
VYGGIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQS
PYTSLSHFL---

---NNFRFNRNSVQRNAKPAQQQRSAQHAKPAQHGKPAQHATQQQHAKPAQNAKPVQHNA
QQQHAKPAQHAAQQQHAKPAQHVAQQQQQEHQRQEALAKQQARIRQQNLVKQAAQKKXXS
QRAASKNQ
Translated Amino Acid sequence (All Frames) 
Frame A:
llaywnkflq*vlqliksqlefmlmvvli*cildmqmh*dkqen*eif**lvyiqmkklq
kikvhql*mnkkdikqyvhvngqmklqkvhlil*lkniwiv*tvifvymvkifqlvqmvk
mfmealkrvvnldssnvlkvfqqpswlvvcyyapktifkmfqvskphhwevsiqtfytnn
hhtqvyhis---

---NNFRFNRNSVQRNAKPAQQQRSAQHAKPAQHGKPAQHATQQQHAKPAQNAKPVQHNA
QQQHAKPAQHAAQQQHAKPAQHVAQQQQQEHQRQEALAKQQARIRQQNLVKQAAQKKXXS
QRAASKNQ

Frame B:
cwptgtsfyneyyn**kan*slc*wlf*fnafwtckciktskrirryfsswctyr*rnck
k*rstsye*trki*sstcm*mgr*scrrctlyfn*rifg*fkl*flctw*ryfswcrw*r
clwrh*kew*i*ihqty*rcfnnrvgwsyvimhqrpsskcfr*anltigrcqskrftqti
tihkfitfp---

---tisdstvtlfnvmpnqlnnkdllnmpnqlnmvnqlnmplnnnmpnqpkmlnqfnitp
nnnmpnqlnmplnnnmpnqlnmslnnnnkntkdkklsqnnklvldnkilsnklpkrnxpl
nvlplki

Frame C:
VGLLEQVFTMSTTTNKKPIRVYVDGCFDLMHFGHANALRQARELGDILVVGVHTDEEIAK
NKGPPVMNEQERYKAVRACKWADEVAEGAPYTLTEEYLDSLNCDFCVHGEDISVGADGKD
VYGGIKKSGKFRFIKRTEGVSTTELVGRMLLCTKDHLQNVSGEQTSPLGGVNPNVLHKQS
PYTSLSHFL---

---qfqiqp*lcst*cqtssttkicstcqtsstw*tsstchstttcqtspkc*tsst*rp
tttcqtsstcrstttcqtsstcrsttttrtpktrssrkttssy*ttkscqtscpketxls
tccl*ks
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA214 (CHA214Q) /CSM/CH/CHA2-A/CHA214Q.Seq.d/                       1402   0.0
VFI626 (VFI626Q) /CSM/VF/VFI6-B/VFI626Q.Seq.d/                       1120   0.0
VFN722 (VFN722Q) /CSM/VF/VFN7-A/VFN722Q.Seq.d/                       1090   0.0
VFG581 (VFG581Q) /CSM/VF/VFG5-D/VFG581Q.Seq.d/                       1090   0.0
VFF663 (VFF663Q) /CSM/VF/VFF6-C/VFF663Q.Seq.d/                       1090   0.0
VFF530 (VFF530Q) /CSM/VF/VFF5-B/VFF530Q.Seq.d/                       1090   0.0
VFE361 (VFE361Q) /CSM/VF/VFE3-C/VFE361Q.Seq.d/                       1090   0.0
VFE318 (VFE318Q) /CSM/VF/VFE3-A/VFE318Q.Seq.d/                       1090   0.0
AFF396 (AFF396Q) /CSM/AF/AFF3-D/AFF396Q.Seq.d/                       1090   0.0
AFF388 (AFF388Q) /CSM/AF/AFF3-D/AFF388Q.Seq.d/                       1090   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X57149|X57149.1 D.discoideum EB4 gene for prespore vesicle protein.   313   e-152  5
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.   313   e-150  7
M11028|M11028.1 Slime mold (D.discoideum) prespore EB4 gene.       78   7e-22  2
BG140383|BG140383.1 EST480825 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP17G1 5' sequence, mRNA sequence.    92   4e-16  3
BG131085|BG131085.1 EST463977 tomato crown gall Lycopersicon esculentum cDNA clone cTOE2D11 5' sequence, mRNA sequence.    92   6e-16  3
AW217896|AW217896.1 EST296610 tomato flower buds, anthesis, Cornell University Lycopersicon esculentum cDNA clone cTOD1C15, mRNA sequence.    92   5e-15  2
AI778006|AI778006.1 EST258885 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES3N17, mRNA sequence.    92   6e-15  2
AW217261|AW217261.1 EST295975 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC89G22, mRNA sequence.    92   6e-15  2
AW219042|AW219042.1 EST301524 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX2H3, mRNA sequence.    92   7e-15  2
BG138134|BG138134.1 EST478576 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP8K18 5' sequence, mRNA sequence.    92   7e-15  2
dna update 2004. 9.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BZ4) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   370   e-101
AL663030_9(AL663030|pid:none) Mouse DNA sequence from clone RP23...   200   5e-50
AK166319_1(AK166319|pid:none) Mus musculus mammary gland RCB-052...   200   5e-50
(Q5EA75) RecName: Full=Ethanolamine-phosphate cytidylyltransfera...   198   2e-49
AL663030_11(AL663030|pid:none) Mouse DNA sequence from clone RP2...   196   1e-48
AY189524_1(AY189524|pid:none) Mus musculus CTP:ethanolaminephosp...   194   4e-48
BC088018_1(BC088018|pid:none) Xenopus tropicalis phosphate cytid...   193   8e-48
AE014134_2379(AE014134|pid:none) Drosophila melanogaster chromos...   192   2e-47
AC087079_11(AC087079|pid:none) Caenorhabditis elegans cosmid Y37...   188   3e-46
AY813109_1(AY813109|pid:none) Schistosoma japonicum SJCHGC06128 ...   186   1e-45
protein update 2009. 3.28
PSORT 

 psg: 0.75  gvh: 0.53  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for CHA214 is nuc
5' end seq. ID CHA214F
5' end seq. 
>CHA214F.Seq
CTGTTGGCCTACTGGAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTA
GAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGAC
AAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAA
AAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTA
AATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATA
GTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAG
ATGTTTATGGAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTG
TTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATG
TTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAAT
CACCATACACAAGTTTATCACATTTCCTCNNNNNNNNNN
Length of 5' end seq. 579
3' end seq. ID CHA214Z
3' end seq. 
>CHA214Z.Seq
NNNNNNNNNNAACAATTTCAGATTCAACCGTAACTCTGTTCAACGTAATGCCAAACCAGC
TCAACAACAAAGATCTGCTCAACATGCCAAACCAGCTCAACATGGTAAACCAGCTCAACA
TGCCACTCAACAACAACATGCCAAACCAGCCCAAAATGCTAAACCAGTTCAACATAACGC
CCAACAACAACATGCCAAACCAGCTCAACATGCCGCTCAACAACAACATGCCAAACCAGC
TCAACATGTCGCTCAACAACAACAACAAGAACACCAAAGACAAGAAGCTCTCGCAAAACA
ACAAGCTCGTATTAGACAACAAAATCTTGTCAAACAAGCTGCCCAAAAGAAACNNNCCTC
TCAACGTGCTGCCTCTAAAAATCAA
Length of 3' end seq. 385
Connected seq. ID CHA214P
Connected seq. 
>CHA214P.Seq
CTGTTGGCCTACTGGAACAAGTTTTTACAATGAGTACTACAACTAATAAAAAGCCAATTA
GAGTTTATGTTGATGGTTGTTTTGATTTAATGCATTTTGGACATGCAAATGCATTAAGAC
AAGCAAGAGAATTAGGAGATATTTTAGTAGTTGGTGTACATACAGATGAAGAAATTGCAA
AAAATAAAGGTCCACCAGTTATGAATGAACAAGAAAGATATAAAGCAGTACGTGCATGTA
AATGGGCAGATGAAGTTGCAGAAGGTGCACCTTATACTTTAACTGAAGAATATTTGGATA
GTTTAAACTGTGATTTTTGTGTACATGGTGAAGATATTTCAGTTGGTGCAGATGGTAAAG
ATGTTTATGGAGGCATTAAAAAGAGTGGTAAATTTAGATTCATCAAACGTACTGAAGGTG
TTTCAACAACCGAGTTGGTTGGTCGTATGTTATTATGCACCAAAGACCATCTTCAAAATG
TTTCAGGTGAGCAAACCTCACCATTGGGAGGTGTCAATCCAAACGTTTTACACAAACAAT
CACCATACACAAGTTTATCACATTTCCTC----------AACAATTTCAGATTCAACCGT
AACTCTGTTCAACGTAATGCCAAACCAGCTCAACAACAAAGATCTGCTCAACATGCCAAA
CCAGCTCAACATGGTAAACCAGCTCAACATGCCACTCAACAACAACATGCCAAACCAGCC
CAAAATGCTAAACCAGTTCAACATAACGCCCAACAACAACATGCCAAACCAGCTCAACAT
GCCGCTCAACAACAACATGCCAAACCAGCTCAACATGTCGCTCAACAACAACAACAAGAA
CACCAAAGACAAGAAGCTCTCGCAAAACAACAAGCTCGTATTAGACAACAAAATCTTGTC
AAACAAGCTGCCCAAAAGAAACNNNCCTCTCAACGTGCTGCCTCTAAAAATCAA
Length of connected seq. 944
Full length Seq ID -
Full length Seq. -
Length of full length seq. -