>CHA189 (CHA189Q) /CSM/CH/CHA1-D/CHA189Q.Seq.d/
ACTGTTGGCCTACTGGTATTGTCATAATAATATTATAAAATTCAAAGTATGTCTAAAGTA
GTCCGTAGTAGTAAATATCGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTAT
CAAAACTTAAAAGTTACCAAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGA
TACTTTGGTGTTATTTGGGATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAG
GCTTCAGGTAAAACCACATCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGAT
ATTGCTTTCCACCCATTCAATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATT
TGTATTTGGGGTATCCCAGAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACT
TTATCTGGTCACAAGAGAAAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTT
GCCGTTACCTCATCTGGTGATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAAT
TTAACCACTGTTGAAGGTCACTCTGATATGATCACTTCATGCGAATGGAATCACAATGGT
AGXXXXXXXXXXTAAAGGTATCACTTTTGATAGTGGTGGTATTTCAATTAAACCATCAGC
AAATATGGGTTTAATGAAGGGTGATATGGGTGGTGCTGCCACTGCTGTCTCTGCAATGTT
TGGTGTTGCTTCATTGGGTTTAAAGGTCAATTTAATTACAATCACTCCATTATGTGAAAA
TATGCCATCAGGTAAAGCAACTAAACCAGGTGATATCCTTACCGCTGCAAATGGTAAAAC
CGTTGAAGTCGACAATACCGATGCCGAGGGTCGTTTAATCTTGGGTGATGCTTTACATTA
TGCTTGTTCATTCAAACCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGA
TGTTGCCTTGGGTCAACATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCA
GTTAAATGAATGTGGTAACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGA
ATATCGTAAACAAATGGAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTC
AGGTGGTGCTTGTTGTGCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTG
GTCTCACCTTGATATTGCTGGTGTTATGTCATCATCTGAAGAGGTCCATACATTAGAAAA
GGTATGACNGAAACCAACTCGACTTAATAGAATTGCAAAAAGAATCAACAA

tvgllvls**YYKIQSMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTR
YFGVIWDAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNI
CIWGIPEGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKN
LTTVEGHSDMITSCEWNHNG---

---KGITFDSGGISIKPSANMGLMKGDMGGAATAVSAMFGVASLGLKVNLITITPLCENM
PSGKATKPGDILTAANGKTVEVDNTDAEGRLILGDALHYACSFKPTHIIDIATLTGAIDV
ALGQHYAGCFTTTDSLWDQLNECGNISGERLWRMPLIPEYRKQMETSKVADLINSAGRSG
GACCAAGFLKEFITADQSWSHLDIAGVMSSSEEVHTLEKV*xkptrlnriakrin

Frame A:
tvgllvls**YYKIQSMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTR
YFGVIWDAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNI
CIWGIPEGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKN
LTTVEGHSDMITSCEWNHNG---

---*ryhf**wwyfn*tiskygfneg*ygwcchcclcnvwccfigfkgqfnynhsim*ky
air*sn*tr*ypyrckw*nr*srqyrcrgsfnlg*cftlclfiqtnsyh*yryldwchrc
clgstlcwlfynhrltlgsvk*mw*h*w*kimenaidsris*tngnlkscrfnqfcwsfr
wcllccwfp*rihysrsilvsp*ycwcyvii*rgpyirkgmtetnst**nckknqq

Frame B:
llaywychnniikfkvclk*svvvnivmflqhnqkkksaikt*klpnqhgivitlqptld
tlvlfgmlqvvvhsllfhmrlqvkphqfhysmvirvqf*illsthsmktslvqflkivtf
vfgvsqkvv*ptqsqphsklylvtrerlvpshsiqllimlplphlvis*lklgmlnkvri
*pllkvtli*slhangitmv---

---KGITFDSGGISIKPSANMGLMKGDMGGAATAVSAMFGVASLGLKVNLITITPLCENM
PSGKATKPGDILTAANGKTVEVDNTDAEGRLILGDALHYACSFKPTHIIDIATLTGAIDV
ALGQHYAGCFTTTDSLWDQLNECGNISGERLWRMPLIPEYRKQMETSKVADLINSAGRSG
GACCAAGFLKEFITADQSWSHLDIAGVMSSSEEVHTLEKV*xkptrlnriakrin

Frame C:
cwptgiviiil*nskyv*ssp***isscfcsttkkrrvlsklksyqismg**lrcsqh*i
lwcylgccrwwfircyst*gfr*nhissiiqws*ecsfrycfppiq*kprwfsf*rl*hl
ylgyprrwfnrlnlnptpnfiwsqekgwyhliqssc**ccryliw*fls*nlgc*tr*ef
nhc*rsl*ydhfmrmesqw*---

---kvsllivvvfqlnhqqiwv**rviwvvlpllslqclvllhwv*rsi*lqslhyvkic
hqvkqlnqvislplqmvkplkstipmprvv*swvmlyimlvhsnqlislislp*lvpsml
pwvnimlvvlqppthfgis*mnvvtlvvkdygech*fqnivnkwkpqklpi*sillvvqv
vlvvllvslknslqpinlgltlillvlchhlkrsih*krydxnqldlielqkest

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHA189 (CHA189Q) /CSM/CH/CHA1-D/CHA189Q.Seq.d/                       2492   0.0
AFK487 (AFK487Q) /CSM/AF/AFK4-D/AFK487Q.Seq.d/                       1338   0.0
AFO810 (AFO810Q) /CSM/AF/AFO8-A/AFO810Q.Seq.d/                       1314   0.0
SFH132 (SFH132Q) /CSM/SF/SFH1-B/SFH132Q.Seq.d/                       1310   0.0
CFK183 (CFK183Q) /CSM/CF/CFK1-D/CFK183Q.Seq.d/                       1308   0.0
AFI369 (AFI369Q) /CSM/AF/AFI3-C/AFI369Q.Seq.d/                       1306   0.0
VFJ706 (VFJ706Q) /CSM/VF/VFJ7-A/VFJ706Q.Seq.d/                       1302   0.0
VFG447 (VFG447Q) /CSM/VF/VFG4-B/VFG447Q.Seq.d/                       1298   0.0
AFG522 (AFG522Q) /CSM/AF/AFG5-A/AFG522Q.Seq.d/                       1298   0.0
AFM362 (AFM362Q) /CSM/AF/AFM3-C/AFM362Q.Seq.d/                       1292   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X61480|X61480.1 D.discoideum mRNA for coronin.                    963   0.0    2
CN558054|CN558054.1 tae47c07.y1 Hydra EST Darmstadt I Hydra magnipapillata cDNA 5' similar to SW:AMPL_BOVIN P00727 CYTOSOL AMINOPEPTIDASE ;, mRNA sequence.    36   6e-06  4
AI877971|AI877971.1 fc55g11.y1 Zebrafish WashU MPIMG EST Danio rerio cDNA clone IMAGE:3725348 5' similar to SW:AMPL_BOVIN P00727 CYTOSOL AMINOPEPTIDASE ;, mRNA sequence.    48   7e-05  3
AY583235|AY583235.1 Mycoplasma fermentans strain PG18 putative transport operon, partial sequence; bacteriophage phiMFV1a, complete sequence; thymidine kinase (tmk) gene, complete sequence and leucine aminopeptidase (lap) gene, partial sequence sequence.    48   0.026  4
AR318728|AR318728.1 Sequence 1278 from patent US 6562958.          46   0.20   2
BX215284|BX215284.1 Danio rerio genomic clone DKEY-263C24, genomic survey sequence.    36   0.25   3
AE000026|AE000026.2 Mycoplasma pneumoniae M129 section 54 of 63 of the complete genome.    48   0.36   1
AE009628|AE009628.1 Brucella melitensis strain 16M chromosome I, section 185 of 195 of the complete sequence.    48   0.36   1
AE014504|AE014504.3 Brucella suis 1330 chromosome I section 190 of 190 of the complete genome.    48   0.36   1
BQ732683|BQ732683.1 AGENCOURT_8510850 NICHD XGC Emb4 Xenopus laevis cDNA clone IMAGE:4683974 5', mRNA sequence.    48   0.36   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5V9F0) RecName: Full=Cytosol aminopeptidase;          EC=3.4.1...   396   e-108
S18663(S18663) coronin - slime mold (Dictyostelium discoideum)  ...   382   e-104
BX901913_3(BX901913|pid:none) Zebrafish DNA sequence from clone ...   242   2e-62
BC068707_1(BC068707|pid:none) Xenopus laevis hypothetical protei...   238   3e-61
AB170537_1(AB170537|pid:none) Macaca fascicularis brain cDNA clo...   233   8e-60
AF061738_1(AF061738|pid:none) Homo sapiens leucine aminopeptidas...   233   8e-60
AK022055_1(AK022055|pid:none) Homo sapiens cDNA FLJ11993 fis, cl...   233   8e-60
AJ720335_1(AJ720335|pid:none) Gallus gallus mRNA for hypothetica...   233   8e-60
CT010237_1(CT010237|pid:none) Mus musculus full open reading fra...   232   2e-59
CT010301_1(CT010301|pid:none) Mus musculus full open reading fra...   232   2e-59

 psg: 0.47  gvh: 0.32  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.04
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for CHA189 is cyt

>CHA189F.Seq
ACTGTTGGCCTACTGGTATTGTCATAATAATATTATAAAATTCAAAGTATGTCTAAAGTA
GTCCGTAGTAGTAAATATCGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTAT
CAAAACTTAAAAGTTACCAAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGA
TACTTTGGTGTTATTTGGGATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAG
GCTTCAGGTAAAACCACATCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGAT
ATTGCTTTCCACCCATTCAATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATT
TGTATTTGGGGTATCCCAGAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACT
TTATCTGGTCACAAGAGAAAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTT
GCCGTTACCTCATCTGGTGATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAAT
TTAACCACTGTTGAAGGTCACTCTGATATGATCACTTCATGCGAATGGAATCACAATGGT
AGNNNNNNNNNN

>CHA189Z.Seq
NNNNNNNNNNTAAAGGTATCACTTTTGATAGTGGTGGTATTTCAATTAAACCATCAGCAA
ATATGGGTTTAATGAAGGGTGATATGGGTGGTGCTGCCACTGCTGTCTCTGCAATGTTTG
GTGTTGCTTCATTGGGTTTAAAGGTCAATTTAATTACAATCACTCCATTATGTGAAAATA
TGCCATCAGGTAAAGCAACTAAACCAGGTGATATCCTTACCGCTGCAAATGGTAAAACCG
TTGAAGTCGACAATACCGATGCCGAGGGTCGTTTAATCTTGGGTGATGCTTTACATTATG
CTTGTTCATTCAAACCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGATG
TTGCCTTGGGTCAACATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCAGT
TAAATGAATGTGGTAACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGAAT
ATCGTAAACAAATGGAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTCAG
GTGGTGCTTGTTGTGCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTGGT
CTCACCTTGATATTGCTGGTGTTATGTCATCATCTGAAGAGGTCCATACATTAGAAAAGG
TATGACNGAAACCAACTCGACTTAATAGAATTGCAAAAAGAATCAACAA

>CHA189P.Seq
ACTGTTGGCCTACTGGTATTGTCATAATAATATTATAAAATTCAAAGTATGTCTAAAGTA
GTCCGTAGTAGTAAATATCGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTAT
CAAAACTTAAAAGTTACCAAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGA
TACTTTGGTGTTATTTGGGATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAG
GCTTCAGGTAAAACCACATCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGAT
ATTGCTTTCCACCCATTCAATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATT
TGTATTTGGGGTATCCCAGAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACT
TTATCTGGTCACAAGAGAAAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTT
GCCGTTACCTCATCTGGTGATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAAT
TTAACCACTGTTGAAGGTCACTCTGATATGATCACTTCATGCGAATGGAATCACAATGGT
AG----------TAAAGGTATCACTTTTGATAGTGGTGGTATTTCAATTAAACCATCAGC
AAATATGGGTTTAATGAAGGGTGATATGGGTGGTGCTGCCACTGCTGTCTCTGCAATGTT
TGGTGTTGCTTCATTGGGTTTAAAGGTCAATTTAATTACAATCACTCCATTATGTGAAAA
TATGCCATCAGGTAAAGCAACTAAACCAGGTGATATCCTTACCGCTGCAAATGGTAAAAC
CGTTGAAGTCGACAATACCGATGCCGAGGGTCGTTTAATCTTGGGTGATGCTTTACATTA
TGCTTGTTCATTCAAACCAACTCATATCATTGATATCGCTACCTTGACTGGTGCCATCGA
TGTTGCCTTGGGTCAACATTATGCTGGTTGTTTTACAACCACCGACTCACTTTGGGATCA
GTTAAATGAATGTGGTAACATTAGTGGTGAAAGATTATGGAGAATGCCATTGATTCCAGA
ATATCGTAAACAAATGGAAACCTCAAAAGTTGCCGATTTAATCAATTCTGCTGGTCGTTC
AGGTGGTGCTTGTTGTGCTGCTGGTTTCCTTAAAGAATTCATTACAGCCGATCAATCTTG
GTCTCACCTTGATATTGCTGGTGTTATGTCATCATCTGAAGAGGTCCATACATTAGAAAA
GGTATGACNGAAACCAACTCGACTTAATAGAATTGCAAAAAGAATCAACAA