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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFK274
Library CF
(Link to library)
Clone ID CFK274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15673-1
Original site URL
Representative seq. ID CFK274P
(Link to Original site)
Representative DNA sequence 
>CFK274 (CFK274Q) /CSM/CF/CFK2-D/CFK274Q.Seq.d/
ATTTTATTTATTTATTTATTTATTTATTAATTTATTTTTTTTGTTTATCTTTATAATTTA
TTAAAAAAAAAAAATAAAAAAAAANGAANTNGAAAAAAAAAAGGTAATTNGGAAAAAAAA
AAXXXXXXXXXXTTGCAATTTCGATGGATTTCAATTGGTTCATCGTTCAGCCAAAAATCA
TGCACAAGAAACCTCCACACCAGCCTATAGTAACCTAATTACATTACTCTCCTCGGGTGA
TATGGAAACCCAATTGAATACATTGACCCTATTCAATTGTCTATTGGATAATGCACCAAA
TCCAAGAAAATCAGAGAAACTACTATCACGTTGGCAACAATTGGGTATTATAAAGATATT
GAAATCACAAGAACATGTAACTCATTCGGATTTTCGTACTCAGATAGCACGTTTCCAAGC
AAACTCTGGTTTTGGTATCGATGGTTCTGGTAGGAAGAGAACTTTGACTCGTCAATTGTC
AACTCAAGAATTGGAATTTCAATTGCACCAATTCCGTGAACAACAACCACTAATCTCTCT
ATTAACTAGTGAATTGAAATTCCTAAGAAATGCAATTAAATCAGCCATTGAAAATGGTTC
CTACATAAACTATAGAGCACCCACCGAACGTTACGATGAATATTCTCAAAGAAAATTAGA
AATGATTGGTGATTCTCCAACAAATCTTCAATTCTTAAAAAGAAATGATAAATTTACCAA
TGCATTTAGAAAATCAATGT
sequence update 2001.11.22
Translated Amino Acid sequence 
ilfiylfiy*FIFFVYLYNLLKKKNKKKXXXKKKGNXEKK---

---CNFDGFQLVHRSAKNHAQETSTPAYSNLITLLSSGDMETQLNTLTLFNCLLDNAPNP
RKSEKLLSRWQQLGIIKILKSQEHVTHSDFRTQIARFQANSGFGIDGSGRKRTLTRQLST
QELEFQLHQFREQQPLISLLTSELKFLRNAIKSAIENGSYINYRAPTERYDEYSQRKLEM
IGDSPTNLQFLKRNDKFTNAFRKSM
Translated Amino Acid sequence (All Frames) 
Frame A:
ilfiylfiy*FIFFVYLYNLLKKKNKKKXXXKKKGNXEKK---

---lqfrwisigssfsqksctrnlhtsl**pnyitllg*ygnpieyidpiqlsig*ctks
kkirettitlatigyykdieitrtcnsfgfsysdstfpsklwfwyrwfw*eenfdssivn
srigisiapip*ttttnlsin**ieipkkcn*ish*kwflhkl*sthrtlr*ifskkirn
dw*fsnkssilkkk**iyqci*kin

Frame B:
fylfiylfinlfflfifiiy*kkkikkxexekkkvixkkk---

---CNFDGFQLVHRSAKNHAQETSTPAYSNLITLLSSGDMETQLNTLTLFNCLLDNAPNP
RKSEKLLSRWQQLGIIKILKSQEHVTHSDFRTQIARFQANSGFGIDGSGRKRTLTRQLST
QELEFQLHQFREQQPLISLLTSELKFLRNAIKSAIENGSYINYRAPTERYDEYSQRKLEM
IGDSPTNLQFLKRNDKFTNAFRKSM

Frame C:
fiylfiylliyffclsl*fikkkk*kkxxxkkkr*xgkkk---

---aismdfnwfivqpkimhkkpphqpivt*lhysprviwkpn*ih*pysivywimhqiq
enqrnyyhvgnnwvl*ry*nhknm*lirifvlr*hvskqtlvlvsmvlvgrel*lvncql
knwnfnctnsvnnnh*sly*lvn*ns*emqlnqplkmvpt*tiehppnvtmnilken*k*
lvilqqifns*keminlpmhlenqc
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFK274 (CFK274Q) /CSM/CF/CFK2-D/CFK274Q.Seq.d/                       1205   0.0
AFG195 (AFG195Q) /CSM/AF/AFG1-D/AFG195Q.Seq.d/                       1205   0.0
AFO665 (AFO665Q) /CSM/AF/AFO6-C/AFO665Q.Seq.d/                       1142   0.0
SFK553 (SFK553Q) /CSM/SF/SFK5-C/SFK553Q.Seq.d/                       1078   0.0
AFO271 (AFO271Q) /CSM/AF/AFO2-C/AFO271Q.Seq.d/                       1068   0.0
SFB421 (SFB421Q) /CSM/SF/SFB4-A/SFB421Q.Seq.d/                        398   e-110
VHM733 (VHM733Q) /CSM/VH/VHM7-B/VHM733Q.Seq.d/                         36   0.73
VSE728 (VSE728Q) /CSM/VS/VSE7-B/VSE728Q.Seq.d/                         34   2.9
VSB134 (VSB134Q) /CSM/VS/VSB1-B/VSB134Q.Seq.d/                         34   2.9
VHO528 (VHO528Q) /CSM/VH/VHO5-B/VHO528Q.Seq.d/                         34   2.9
own update 2004. 7.19
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AB082544|AB082544.1 Dictyostelium discoideum forC gene for formin homology protein C, complete cds.  1197   0.0    1
AC097023|AC097023.5 Rattus norvegicus clone CH230-35L12, WORKING DRAFT SEQUENCE, 7 unordered pieces.    36   0.54   3
AC126822|AC126822.2 Rattus norvegicus clone CH230-9D8, *** SEQUENCING IN PROGRESS ***, 13 unordered pieces.    42   0.59   8
AC107397|AC107397.5 Homo sapiens BAC clone RP11-703C14 from 4, complete sequence.    36   1.9    6
AC023245|AC023245.5 Homo sapiens chromosome 8, clone RP11-25D10, complete sequence.    32   2.6    7
AQ536927|AQ536927.1 RPCI-11-325G24.TJ RPCI-11 Homo sapiens genomic clone RPCI-11-325G24, genomic survey sequence.    44   2.7    1
AC098656|AC098656.2 Homo sapiens chromosome 1 clone RP11-297I23, complete sequence.    44   2.7    1
AC107491|AC107491.6 Rattus norvegicus clone CH230-86E8, *** SEQUENCING IN PROGRESS ***, 8 unordered pieces.    44   2.7    1
AC095615|AC095615.5 Rattus norvegicus clone CH230-8C19, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44   2.7    1
AC021872|AC021872.22 Homo sapiens 12 BAC RP11-253F9 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38   2.9    5
dna update 2003.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXK2) RecName: Full=Formin-G;                                       99   1e-19
AF083031_50(AF083031|pid:none) Guillardia theta nucleomorph chro...    35   2.4
CP000721_1294(CP000721|pid:none) Clostridium beijerinckii NCIMB ...    35   3.1
AE017340_2310(AE017340|pid:none) Idiomarina loihiensis L2TR, com...    34   4.0
AB465596_1(AB465596|pid:none) Nilaparvata lugens mRNA for lipoph...    34   4.0
AP008934_994(AP008934|pid:none) Staphylococcus saprophyticus sub...    34   5.2
AP008937_1820(AP008937|pid:none) Lactobacillus fermentum IFO 395...    33   6.9
(Q5RDG2) RecName: Full=Armadillo repeat-containing X-linked prot...    33   6.9
(Q6P1M9) RecName: Full=Armadillo repeat-containing X-linked prot...    33   8.9
AK023031_1(AK023031|pid:none) Homo sapiens cDNA FLJ12969 fis, cl...    33   8.9
protein update 2009. 5.17
PSORT 

 psg: 0.94  gvh: 0.26  alm: 0.48  top: 1.07  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for CFK274 is nuc
5' end seq. ID CFK274F
5' end seq. 
>CFK274F.Seq
ATTTTATTTATTTATTTATTTATTTATTAATTTATTTTTTTTGTTTATCTTTATAATTTA
TTAAAAAAAAAAAATAAAAAAAAANGAANTNGAAAAAAAAAAGGTAATTNGGAAAAAAAA
AANNNNNNNNNN
Length of 5' end seq. 132
3' end seq. ID CFK274Z
3' end seq. 
>CFK274Z.Seq
NNNNNNNNNNTTGCAATTTCGATGGATTTCAATTGGTTCATCGTTCAGCCAAAAATCATG
CACAAGAAACCTCCACACCAGCCTATAGTAACCTAATTACATTACTCTCCTCGGGTGATA
TGGAAACCCAATTGAATACATTGACCCTATTCAATTGTCTATTGGATAATGCACCAAATC
CAAGAAAATCAGAGAAACTACTATCACGTTGGCAACAATTGGGTATTATAAAGATATTGA
AATCACAAGAACATGTAACTCATTCGGATTTTCGTACTCAGATAGCACGTTTCCAAGCAA
ACTCTGGTTTTGGTATCGATGGTTCTGGTAGGAAGAGAACTTTGACTCGTCAATTGTCAA
CTCAAGAATTGGAATTTCAATTGCACCAATTCCGTGAACAACAACCACTAATCTCTCTAT
TAACTAGTGAATTGAAATTCCTAAGAAATGCAATTAAATCAGCCATTGAAAATGGTTCCT
ACATAAACTATAGAGCACCCACCGAACGTTACGATGAATATTCTCAAAGAAAATTAGAAA
TGATTGGTGATTCTCCAACAAATCTTCAATTCTTAAAAAGAAATGATAAATTTACCAATG
CATTTAGAAAATCAATGT
Length of 3' end seq. 618
Connected seq. ID CFK274P
Connected seq. 
>CFK274P.Seq
ATTTTATTTATTTATTTATTTATTTATTAATTTATTTTTTTTGTTTATCTTTATAATTTA
TTAAAAAAAAAAAATAAAAAAAAANGAANTNGAAAAAAAAAAGGTAATTNGGAAAAAAAA
AA----------TTGCAATTTCGATGGATTTCAATTGGTTCATCGTTCAGCCAAAAATCA
TGCACAAGAAACCTCCACACCAGCCTATAGTAACCTAATTACATTACTCTCCTCGGGTGA
TATGGAAACCCAATTGAATACATTGACCCTATTCAATTGTCTATTGGATAATGCACCAAA
TCCAAGAAAATCAGAGAAACTACTATCACGTTGGCAACAATTGGGTATTATAAAGATATT
GAAATCACAAGAACATGTAACTCATTCGGATTTTCGTACTCAGATAGCACGTTTCCAAGC
AAACTCTGGTTTTGGTATCGATGGTTCTGGTAGGAAGAGAACTTTGACTCGTCAATTGTC
AACTCAAGAATTGGAATTTCAATTGCACCAATTCCGTGAACAACAACCACTAATCTCTCT
ATTAACTAGTGAATTGAAATTCCTAAGAAATGCAATTAAATCAGCCATTGAAAATGGTTC
CTACATAAACTATAGAGCACCCACCGAACGTTACGATGAATATTCTCAAAGAAAATTAGA
AATGATTGGTGATTCTCCAACAAATCTTCAATTCTTAAAAAGAAATGATAAATTTACCAA
TGCATTTAGAAAATCAATGT
Length of connected seq. 730
Full length Seq ID -
Full length Seq. -
Length of full length seq. -