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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFK240
Library CF
(Link to library)
Clone ID CFK240
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13873-1
Original site URL
Representative seq. ID CFK240P
(Link to Original site)
Representative DNA sequence 
>CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATAXXXXXXXXXXTGGATGCTATCAATCATT
CGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATC
CAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTA
GTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAA
TTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAG
CTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAA
GAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAA
TCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTT
GTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTG
GTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAAT
ATAATAGATTAATGAAAATTGAAGAAGAACTNAAAAACTTCATAGATCAAATGGTAATTC
ATTTGAGTATGCTGGNAAGAGTTTTATCATTTT
sequence update 2001.11.22
Translated Amino Acid sequence 
flif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFEL
RDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAI
VGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGN---

---DAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRY
LEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVN
SLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAP
CRFERLSKYNRLMKIEEELKNFIDQMVIHLSMLXRVLSF
Translated Amino Acid sequence (All Frames) 
Frame A:
ifniliiiilkney*in*s*rnfg**r*snirs*smdk*rnvfsqstkwsinwys*si*i
krw**kslfwiwcfecnc*ck*fnitnieryes**sksnr**nd*igwyke*iktwvqcn
rwcingsgkssrfy*rcalvplhftigwk**ysftstmf*cy*wwktcr*---

---wmlsiiraikmllklvlilllqnfgiqrlqnmiwisri*dphi*slv*ivvsn*cld
i*rf*ksiqlhslkihlvkmigkimvklqlqlvikfkslvmiyfvqiqkelkkqflrkl*
ihyy*k*iksvh*lkllklqnlqkklvgvv*lvivqvklmiisllil*lvlvlvk*nlvh
lvdlkdyqniid**klkknxkts*ikw*fi*vcwxefyhf

Frame B:
flif**l*f*KMSIESIKAREILDSRGNPTLEVEVWTSEGMFSAKVPSGASTGIHEAFEL
RDGDKSRYFGYGVLNAIVNVNNLISPTLKGMRVDNQKAIDDKMIELDGTKNKSKLGSNAI
VGVSMAVARAAASIKGVPLYRYISQLAGNSNIRLPVPCFNVINGGKHAGN---

---gcyqsfglsrcy*nwy*fccfrileskdfki*ygfqeyriltfev*fk*w*ainv*i
frdfkkvsncil*rsiw*r*lgksw*nyscnw**nsnhw**ftlyksrkn*ksnf*enck
fiiienksnrytn*ny*scktckrswlglfs*ssfr*n**sfhc*scswswyw*nkiwct
l*i*kiiki**inen*rrtxklhrsngnsfeyagksfii

Frame C:
f*yfnnynfkk*vlnqlklekfwiveviqh*klkygqvkecfqpkyqvehqlvfmkhln*
emvikvvildmvf*mqllm*ii*yhqh*kv*eliikkq*mik*lnwmvqrinqnlgpmqs
lvyqwqwqeqplllkvcpctvtfhnwleivifvyqyhvlmllmvenmqvi---

---DAINHSGYQDVIKIGIDSAASEFWNPKTSKYDMDFKNIGSSHLKSSLNSGEQLMSRY
LEILKKYPIAFFEDPFGEDDWENHGKITAAIGNKIQIIGDDLLCTNPERIKKAISEKTVN
SLLLKINQIGTLTETIEAAKLAKEAGWGCLVSHRSGETDDHFIADLVVGLGTGEIKSGAP
CRFERLSKYNRLMKIEEELKNFIDQMVIHLSMLXRVLSF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFK240 (CFK240Q) /CSM/CF/CFK2-B/CFK240Q.Seq.d/                       2254   0.0
CFH624 (CFH624Q) /CSM/CF/CFH6-A/CFH624Q.Seq.d/                       1279   0.0
CFJ407 (CFJ407Q) /CSM/CF/CFJ4-A/CFJ407Q.Seq.d/                       1263   0.0
SFJ731 (SFJ731Q) /CSM/SF/SFJ7-B/SFJ731Q.Seq.d/                       1261   0.0
CHB680 (CHB680Q) /CSM/CH/CHB6-D/CHB680Q.Seq.d/                       1251   0.0
CHC316 (CHC316Q) /CSM/CH/CHC3-A/CHC316Q.Seq.d/                       1243   0.0
CHB459 (CHB459Q) /CSM/CH/CHB4-C/CHB459Q.Seq.d/                       1235   0.0
SFC671 (SFC671Q) /CSM/SF/SFC6-C/SFC671Q.Seq.d/                       1227   0.0
CHC156 (CHC156Q) /CSM/CH/CHC1-C/CHC156Q.Seq.d/                       1221   0.0
CFC343 (CFC343Q) /CSM/CF/CFC3-B/CFC343Q.Seq.d/                       1217   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U09736|U09736.1 Entamoeba histolytica HM1:IMSS enolase (enl-1) mRNA, complete cds.    56   1e-25  5
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-16  5
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    58   3e-16  5
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    58   3e-16  5
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    58   3e-16  5
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.           58   3e-15  4
L23324|L23324.1 Schistosoma japonicum enolase mRNA, complete cds.    72   1e-14  4
AE006265|AE006265.1 Lactococcus lactis subsp. lactis IL1403 section 27 of 218 of the complete genome.    44   1e-14  8
AZ690037|AZ690037.1 ENTKP77TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   9e-13  3
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    58   2e-12  4
dna update 2003.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC148219_22(AC148219|pid:none) Medicago truncatula clone mth2-21...   231   2e-98
AK300662_1(AK300662|pid:none) Homo sapiens cDNA FLJ53926 complet...   209   7e-96
Z68318_5(Z68318|pid:none) Caenorhabditis elegans Cosmid T21B10, ...   216   2e-93
JQ1186(JQ1186) phosphopyruvate hydratase (EC 4.2.1.11) - tomato ...   237   7e-61
EF576148_1(EF576148|pid:none) Oryza sativa (indica cultivar-grou...   235   2e-60
AP008216_217(AP008216|pid:none) Oryza sativa (japonica cultivar-...   235   2e-60
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   234   3e-60
FJ664264_1(FJ664264|pid:none) Annona cherimola enolase mRNA, com...   234   3e-60
(Q7RA60) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   234   3e-60
BT070411_1(BT070411|pid:none) Picea sitchensis clone WS02727_L19...   234   4e-60
protein update 2009. 5.17
PSORT 

 psg: 0.56  gvh: 0.44  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for CFK240 is nuc
5' end seq. ID CFK240F
5' end seq. 
>CFK240F.Seq
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATANNNNNNNNNN
Length of 5' end seq. 522
3' end seq. ID CFK240Z
3' end seq. 
>CFK240Z.Seq
NNNNNNNNNNTGGATGCTATCAATCATTCGGGCTATCAAGATGTTATTAAAATTGGTATT
GATTCTGCTGCTTCAGAATTTTGGAATCCAAAGACTTCAAAATATGATATGGATTTCAAG
AATATAGGATCCTCACATTTGAAGTCTAGTTTAAATAGTGGTGAGCAATTAATGTCTAGA
TATTTAGAGATTTTAAAAAAGTATCCAATTGCATTCTTTGAAGATCCATTTGGTGAAGAT
GATTGGGAAAATCATGGTAAAATTACAGCTGCAATTGGTAATAAAATTCAAATCATTGGT
GATGATTTACTTTGTACAAATCCAGAAAGAATTAAAAAAGCAATTTCTGAGAAAACTGTA
AATTCATTATTATTGAAAATAAATCAAATCGGTACACTAACTGAAACTATTGAAGCTGCA
AAACTTGCAAAAGAAGCTGGTTGGGGTTGTTTAGTTAGTCATCGTTCAGGTGAAACTGAT
GATCATTTCATTGCTGATCTTGTAGTTGGTCTTGGTACTGGTGAAATAAAATCTGGTGCA
CCTTGTAGATTTGAAAGATTATCAAAATATAATAGATTAATGAAAATTGAAGAAGAACTN
AAAAACTTCATAGATCAAATGGTAATTCATTTGAGTATGCTGGNAAGAGTTTTATCATTT
T
Length of 3' end seq. 661
Connected seq. ID CFK240P
Connected seq. 
>CFK240P.Seq
ATTTTTAATATTTTAATAATTATAATTTTAAAAAATGAGTATTGAATCAATTAAAGCTAG
AGAAATTTTGGATAGTAGAGGTAATCCAACATTAGAAGTTGAAGTATGGACAAGTGAAGG
AATGTTTTCAGCCAAAGTACCAAGTGGAGCATCAACTGGTATTCATGAAGCATTTGAATT
AAGAGATGGTGATAAAAGTCGTTATTTTGGATATGGTGTTTTGAATGCAATTGTTAATGT
AAATAATTTAATATCACCAACATTGAAAGGTATGAGAGTTGATAATCAAAAAGCAATAGA
TGATAAAATGATTGAATTGGATGGTACAAAGAATAAATCAAAACTTGGGTCCAATGCAAT
CGTTGGTGTATCAATGGCAGTGGCAAGAGCAGCCGCTTCTATTAAAGGTGTGCCCTTGTA
CCGTTACATTTCACAATTGGCTGGAAATAGTAATATTCGTTTACCAGTACCATGTTTTAA
TGTTATTAATGGTGGAAAACATGCAGGTAATA----------TGGATGCTATCAATCATT
CGGGCTATCAAGATGTTATTAAAATTGGTATTGATTCTGCTGCTTCAGAATTTTGGAATC
CAAAGACTTCAAAATATGATATGGATTTCAAGAATATAGGATCCTCACATTTGAAGTCTA
GTTTAAATAGTGGTGAGCAATTAATGTCTAGATATTTAGAGATTTTAAAAAAGTATCCAA
TTGCATTCTTTGAAGATCCATTTGGTGAAGATGATTGGGAAAATCATGGTAAAATTACAG
CTGCAATTGGTAATAAAATTCAAATCATTGGTGATGATTTACTTTGTACAAATCCAGAAA
GAATTAAAAAAGCAATTTCTGAGAAAACTGTAAATTCATTATTATTGAAAATAAATCAAA
TCGGTACACTAACTGAAACTATTGAAGCTGCAAAACTTGCAAAAGAAGCTGGTTGGGGTT
GTTTAGTTAGTCATCGTTCAGGTGAAACTGATGATCATTTCATTGCTGATCTTGTAGTTG
GTCTTGGTACTGGTGAAATAAAATCTGGTGCACCTTGTAGATTTGAAAGATTATCAAAAT
ATAATAGATTAATGAAAATTGAAGAAGAACTNAAAAACTTCATAGATCAAATGGTAATTC
ATTTGAGTATGCTGGNAAGAGTTTTATCATTTT
Length of connected seq. 1163
Full length Seq ID -
Full length Seq. -
Length of full length seq. -