>CFK230 (CFK230Q) /CSM/CF/CFK2-B/CFK230Q.Seq.d/
ATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAATATCA
ATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTANCAAAANCTAGTAA
TTTAAAAAAAAAAAANCCAAAAAAAAAAXXXXXXXXXXCAGGTCATGAATGCAAACATGA
TGAACATGGTAAAGAATGTTGCGTTAAAAAACGTCATCATGATAGATGTGACCTCAAATG
TAAGAGAGGTTATGAATGTAAAATCAAACATGATGGCTCCAAATGTTGTGTCAAGAGAAC
TCCAAAGAGACCATGTTGTAAACCAAATTCATGTGCCAGAGATGAAAAATGTGTTGCCAC
CAAAGACAGAATCATTTGTGTAAAACCAACTTGTGATAATACTCGTTGTCCTCCAAATTA
CCATTGCATATGTGGTGATAAAATTGATGGTGTAAAATGTGTTCCAGATTGTAAGAAAGC
AAGATGTGATGATGTCGAATGTCCAGATTTCCATAGATGTGTTGAAAGACGTGGTGGTAT
CTTAAGTTGTGAATTTGATCCACCAAGACAACCAAGATCCCTTGATTGGGCTGAAAATGA
AAATGATGATCGTGACTATGATGATCGTGACTATGATGATGATGAATATGATGGTGATTA
TGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGTGATTATGATGATGCCAATTA
TTATGGTGATGATGACTATGATAATGATTGGGACAATGATAATGATTGGGGTAATGATTG
GGATAATGATTGGGACAATGAAGATGGTGATAATTGGAATGATGATGACTTCCAAGATGC
AAATGATGAATGGGACTATTAAGCCATTAATTTTTTAAAAAAAAATAATAATAATATTAA
TACAA

fkkkniksl*yfvhiqikyqxn*kxlklnl*NQFVXKXSNLKKKXPKKK---

---GHECKHDEHGKECCVKKRHHDRCDLKCKRGYECKIKHDGSKCCVKRTPKRPCCKPNS
CARDEKCVATKDRIICVKPTCDNTRCPPNYHCICGDKIDGVKCVPDCKKARCDDVECPDF
HRCVERRGGILSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYDGRDGDYDGD
YDGDYDDANYYGDDDYDNDWDNDNDWGNDWDNDWDNEDGDNWNDDDFQDANDEWDY*ain
flkknnnnint

Frame A:
iqkkky*iivvfcsysn*isxqlkxvkikfvksicxkx**fkkkkxkkk---

---qvmnanmmnmvknvalknvimidvtsnvrevmnvksnmmapnvvsrelqrdhvvnqi
hvpemknvlppktesfv*nqlviilvvlqitiayvviklmv*nvfqivrkqdvmmsnvqi
sidvlkdvvvs*vvnlihqdnqdpliglkmkmmivtmmivtmmmmnmmvimmavtvtmmv
immvimmmpiimvmmtmimigtmimigvmigimigtmkmviigmmmtskmqmmngtikpl
if*kkiiiiliq

Frame B:
fkkkniksl*yfvhiqikyqxn*kxlklnl*NQFVXKXSNLKKKXPKKK---

---rs*mqt**tw*rmlr*ktss**m*pqm*erl*m*nqt*wlqmlcqensketml*tkf
mcqr*kmcchqrqnhlcktnl**yslssklplhmw**n*wckmcsrl*eskm**crmsrf
p*mc*ktwwylkl*i*stkttkip*lg*k*k**s*l**s*l****i*w*l*wp*r*l*w*
l*w*l**cqllw***l***lgq***lg**lg**lgq*rw**le***lprck**mgllsh*
ffkkk***y*y

Frame C:
skkkilnhcsilfifklninxikkx*n*ickinlxqxlvi*kkkxqkk---

---GHECKHDEHGKECCVKKRHHDRCDLKCKRGYECKIKHDGSKCCVKRTPKRPCCKPNS
CARDEKCVATKDRIICVKPTCDNTRCPPNYHCICGDKIDGVKCVPDCKKARCDDVECPDF
HRCVERRGGILSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYDGRDGDYDGD
YDGDYDDANYYGDDDYDNDWDNDNDWGNDWDNDWDNEDGDNWNDDDFQDANDEWDY*ain
flkknnnnint

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK812 (SFK812Q) /CSM/SF/SFK8-A/SFK812Q.Seq.d/                        858   0.0
SFI442 (SFI442Q) /CSM/SF/SFI4-B/SFI442Q.Seq.d/                        858   0.0
SFI183 (SFI183Q) /CSM/SF/SFI1-D/SFI183Q.Seq.d/                        858   0.0
SFH205 (SFH205Q) /CSM/SF/SFH2-A/SFH205Q.Seq.d/                        858   0.0
SFG675 (SFG675Q) /CSM/SF/SFG6-D/SFG675Q.Seq.d/                        858   0.0
SFG605 (SFG605Q) /CSM/SF/SFG6-A/SFG605Q.Seq.d/                        858   0.0
SFG208 (SFG208Q) /CSM/SF/SFG2-A/SFG208Q.Seq.d/                        858   0.0
SFG191 (SFG191Q) /CSM/SF/SFG1-D/SFG191Q.Seq.d/                        858   0.0
SFF313 (SFF313Q) /CSM/SF/SFF3-A/SFF313Q.Seq.d/                        858   0.0
SFD881 (SFD881Q) /CSM/SF/SFD8-D/SFD881Q.Seq.d/                        858   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   858   0.0    4
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   858   0.0    4
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    62   2e-13  3
U25144|U25144.1 Dictyostelium discoideum spore coat protein SP87 (PspD) gene, complete cds.    56   5e-07  2
AC117267|AC117267.2 Dictyostelium discoideum chromosome 2 map 5836255-5862024 strain AX4, complete sequence.    56   1e-06  4
M34546|M34546.1 D.discoideum spore coat 60 (sp60) gene, 5' flank.    62   1e-05  1
BX640507|BX640507.8 Zebrafish DNA sequence from clone DKEY-97O1 in linkage group 14.    34   0.034  2
BX005073|BX005073.7 Zebrafish DNA sequence from clone CH211-129N18.    34   0.034  2
BX323051|BX323051.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    34   0.034  2
BX248127|BX248127.9 Zebrafish DNA sequence from clone CH211-117N7.    34   0.034  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...   338   1e-91
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...   105   2e-21
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...   104   3e-21
AC117267_7(AC117267|pid:none) Dictyostelium discoideum chromosom...    92   3e-17
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    84   5e-15
(Q54QX2) RecName: Full=Probable spore coat protein DDB_G0283555;...    63   2e-08
AF245486_1(AF245486|pid:none) Polysphondylium pallidum spore coa...    50   1e-04
(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        49   2e-04
AM746676_8717(AM746676|pid:none) Sorangium cellulosum 'So ce 56'...    49   3e-04
(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...    47   0.001

 psg: 0.31  gvh: 0.09  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.14  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 32.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for CFK230 is nuc

>CFK230F.Seq
ATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAATATCA
ATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTANCAAAANCTAGTAA
TTTAAAAAAAAAAAANCCAAAAAAAAAANNNNNNNNNN

>CFK230Z.Seq
NNNNNNNNNNCAGGTCATGAATGCAAACATGATGAACATGGTAAAGAATGTTGCGTTAAA
AAACGTCATCATGATAGATGTGACCTCAAATGTAAGAGAGGTTATGAATGTAAAATCAAA
CATGATGGCTCCAAATGTTGTGTCAAGAGAACTCCAAAGAGACCATGTTGTAAACCAAAT
TCATGTGCCAGAGATGAAAAATGTGTTGCCACCAAAGACAGAATCATTTGTGTAAAACCA
ACTTGTGATAATACTCGTTGTCCTCCAAATTACCATTGCATATGTGGTGATAAAATTGAT
GGTGTAAAATGTGTTCCAGATTGTAAGAAAGCAAGATGTGATGATGTCGAATGTCCAGAT
TTCCATAGATGTGTTGAAAGACGTGGTGGTATCTTAAGTTGTGAATTTGATCCACCAAGA
CAACCAAGATCCCTTGATTGGGCTGAAAATGAAAATGATGATCGTGACTATGATGATCGT
GACTATGATGATGATGAATATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGT
GATTATGATGGTGATTATGATGATGCCAATTATTATGGTGATGATGACTATGATAATGAT
TGGGACAATGATAATGATTGGGGTAATGATTGGGATAATGATTGGGACAATGAAGATGGT
GATAATTGGAATGATGATGACTTCCAAGATGCAAATGATGAATGGGACTATTAAGCCATT
AATTTTTTAAAAAAAAATAATAATAATATTAATACAA

>CFK230P.Seq
ATTCAAAAAAAAAAATATTAAATCATTGTAGTATTTTGTTCATATTCAAATTAAATATCA
ATNCAATTAAAAAANGTTAAAATTAAATTTGTAAAATCAATTTGTANCAAAANCTAGTAA
TTTAAAAAAAAAAAANCCAAAAAAAAAA----------CAGGTCATGAATGCAAACATGA
TGAACATGGTAAAGAATGTTGCGTTAAAAAACGTCATCATGATAGATGTGACCTCAAATG
TAAGAGAGGTTATGAATGTAAAATCAAACATGATGGCTCCAAATGTTGTGTCAAGAGAAC
TCCAAAGAGACCATGTTGTAAACCAAATTCATGTGCCAGAGATGAAAAATGTGTTGCCAC
CAAAGACAGAATCATTTGTGTAAAACCAACTTGTGATAATACTCGTTGTCCTCCAAATTA
CCATTGCATATGTGGTGATAAAATTGATGGTGTAAAATGTGTTCCAGATTGTAAGAAAGC
AAGATGTGATGATGTCGAATGTCCAGATTTCCATAGATGTGTTGAAAGACGTGGTGGTAT
CTTAAGTTGTGAATTTGATCCACCAAGACAACCAAGATCCCTTGATTGGGCTGAAAATGA
AAATGATGATCGTGACTATGATGATCGTGACTATGATGATGATGAATATGATGGTGATTA
TGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGTGATTATGATGATGCCAATTA
TTATGGTGATGATGACTATGATAATGATTGGGACAATGATAATGATTGGGGTAATGATTG
GGATAATGATTGGGACAATGAAGATGGTGATAATTGGAATGATGATGACTTCCAAGATGC
AAATGATGAATGGGACTATTAAGCCATTAATTTTTTAAAAAAAAATAATAATAATATTAA
TACAA