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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
CFK216
Library CF
(Link to library)
Clone ID CFK216
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16248-1
Original site URL
Representative seq. ID CFK216P
(Link to Original site)
Representative DNA sequence 
>CFK216 (CFK216Q) /CSM/CF/CFK2-A/CFK216Q.Seq.d/
AAAAGGTAAATAAATAATAAATTAAGATGTTAACATTCAAACAATTAAAAGAAACCGAAA
AAGATTTCACTGTTGATGAAAATAAATTCAGAGTTGCACCAAGCAATGAAACCTTAATGG
AAACTGTCAAGAATTTAGAAGCCAAGAACCACAGAGTTTCAGTCGTTGAAAATGAAGCTG
ATGCTTTAAATTTATTATTAAATGAAATCCCAAAAGGTTCTGAAGTCATGTGCGCTAGCA
GTTGTACCATTGATGAAATCGGTTTCAAGAAAATTTATTTCAGTGATGATTCTCCATTCG
TCAACGTTCATAAAAAGATTTTAGCTTTACCAGCTGATAAACAAGCTGATGCTCGTAAAG
ATGCTTTAACCTCACAATACTTTTTATCATCTGTAACTGCAATCTCCAAAACTGGAAATG
TTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGCGGTTTCACTGCTGCTAAAAATTTAA
TCATCGTTTCTGGTTATAACAAGATTGTTGATTCTGATGAATTAGCCXXXXXXXXXXCTG
TTGGCCTACTGGAAAAGGTAAATAAATAATAAATTAAGATGTTAACATTCAAACAATTAA
AAGAAACCGAAAAAGATTTCNCTGTTGATGAAAATAAATTCAGAGTTGCACCAAGCAATG
AAACCTTAATGGAAACTGTCAAGAATTTAGAAGCCAAGAACCACAGAGTTTCAGTCGTTG
AAAATGAAGCTGATGCTTTAAATTTATTATTAAATGAAATCCCAAAAGGTTCTGAAGTCA
TGTGCGCTAGCAGTTGTACCATTGATGAAATCGGTTTCAAGAAAATTTATTTCAGTGATG
ATTCTCCATTCGTCAACGTTCATAAAAAGATTTTAGCTTTACCAGCTGATAAACAAGCTG
ATGCTCGTAAAGATGCTTTAACCTCACAATACTTTTTATCATCTGTAACTGCAATCTCCA
AAACTGGAAATGTTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGCGGTTTCACTGCTG
CTAAAAATTTAATCATCGTTTCTGGTTATAACAAGATTGTTGANTCTGATGAATTAGCCG
TCCAAAGAACTGAACAATTCTGTTTACCATGTGAAAGTGTTCGTGCTCGTTGGGCTTACA
AAGTTCCAGGTTCAATGATTCAAAATCNTTTGGGTATGAAACATGGTGCTCCAAACAAAC
ATCACATTATCCTCATNAAAAAAATA
sequence update 2001.11.22
Translated Amino Acid sequence 
kvnk**IKMLTFKQLKETEKDFTVDENKFRVAPSNETLMETVKNLEAKNHRVSVVENEAD
ALNLLLNEIPKGSEVMCASSCTIDEIGFKKIYFSDDSPFVNVHKKILALPADKQADARKD
ALTSQYFLSSVTAISKTGNVYVADASGTRVGGFTAAKNLIIVSGYNKIVDSDELA---

---VGLLEKVNK**ikmltfkqlketekdfxvdenkfrvapsnetlmetvknleaknhrv
svveneadalnlllneipkgsevmcassctideigfkkiyfsddspfvnvhkkilalpad
kqadarkdaltsqyflssvtaisktgnvyvadasgtrvggftaaknliivsgynkivxsd
elavqrteqfclpcesvrarwaykvpgsmiqnxlgmkhgapnkhhiilxkki
Translated Amino Acid sequence (All Frames) 
Frame A:
kr*innklrc*hsnn*kkpkkisllmkinselhqamkp*wklsri*kprttefqslkmkl
ml*iyy*mksqkvlkscalavvplmksvsrkfisvmilhsstfikrf*lyqlinklmlvk
ml*phntfyhl*lqspklemfmlptlqvpelavslllki*ssflvitrllilmn*---

---llaywkr*innklrc*hsnn*kkpkkisllmkinselhqamkp*wklsri*kprtte
fqslkmklml*iyy*mksqkvlkscalavvplmksvsrkfisvmilhsstfikrf*lyql
inklmlvkml*phntfyhl*lqspklemfmlptlqvpelavslllki*ssflvitrllxl
mn*pskelnnsvyhvkvfvlvgltkfqvq*fkixwv*nmvlqtnitlssxkk

Frame B:
kgk*iin*dvniqtikrnrkrfhc**k*iqsctkq*nlngncqefrsqepqsfsr*k*s*
cfkfiik*npkrf*shvr*qlyh**nrfqenlfq**fsirqrs*kdfsfts**ts*cs*r
cfnltilfiicncnlqnwkclccrrfryqswrfhcc*kfnhrfwl*qdc*f**is---

---cwptgkgk*iin*dvniqtikrnrkrfxc**k*iqsctkq*nlngncqefrsqepqs
fsr*k*s*cfkfiik*npkrf*shvr*qlyh**nrfqenlfq**fsirqrs*kdfsfts*
*ts*cs*rcfnltilfiicncnlqnwkclccrrfryqswrfhcc*kfnhrfwl*qdc*x*
*isrpkn*tilftm*kcscslglqssrfndsksfgyetwcskqtshyphxkn

Frame C:
kvnk**IKMLTFKQLKETEKDFTVDENKFRVAPSNETLMETVKNLEAKNHRVSVVENEAD
ALNLLLNEIPKGSEVMCASSCTIDEIGFKKIYFSDDSPFVNVHKKILALPADKQADARKD
ALTSQYFLSSVTAISKTGNVYVADASGTRVGGFTAAKNLIIVSGYNKIVDSDELA---

---VGLLEKVNK**ikmltfkqlketekdfxvdenkfrvapsnetlmetvknleaknhrv
svveneadalnlllneipkgsevmcassctideigfkkiyfsddspfvnvhkkilalpad
kqadarkdaltsqyflssvtaisktgnvyvadasgtrvggftaaknliivsgynkivxsd
elavqrteqfclpcesvrarwaykvpgsmiqnxlgmkhgapnkhhiilxkki
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CFK216 (CFK216Q) /CSM/CF/CFK2-A/CFK216Q.Seq.d/                       2333   0.0
SHF652 (SHF652Q) /CSM/SH/SHF6-C/SHF652Q.Seq.d/                       1296   0.0
CHM787 (CHM787Q) /CSM/CH/CHM7-D/CHM787Q.Seq.d/                       1296   0.0
CHG794 (CHG794Q) /CSM/CH/CHG7-D/CHG794Q.Seq.d/                       1296   0.0
VFE226 (VFE226Q) /CSM/VF/VFE2-B/VFE226Q.Seq.d/                       1291   0.0
CHL558 (CHL558Q) /CSM/CH/CHL5-C/CHL558Q.Seq.d/                       1291   0.0
CHL388 (CHL388Q) /CSM/CH/CHL3-D/CHL388Q.Seq.d/                       1291   0.0
CFK289 (CFK289Q) /CSM/CF/CFK2-D/CFK289Q.Seq.d/                       1289   0.0
CHK665 (CHK665Q) /CSM/CH/CHK6-C/CHK665Q.Seq.d/                       1287   0.0
SHK631 (SHK631Q) /CSM/SH/SHK6-B/SHK631Q.Seq.d/                       1285   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AJ310674|AJ310674.1 Dictyostelium discoideum 3B gene for prespore-specific protein 3B,.   682   0.0    2
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   682   0.0    19
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    40   2e-07  17
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    36   1e-06  21
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    42   2e-05  18
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    38   3e-05  11
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    38   4e-05  12
AC135415|AC135415.23 Medicago truncatula clone mth2-10b9, WORKING DRAFT SEQUENCE, 2 ordered pieces.    40   9e-05  6
AF538053|AF538053.1 Monosiga brevicollis mitochondrion, complete genome.    34   9e-05  11
AC136792|AC136792.1 Rattus norvegicus clone CH230-405G3, *** SEQUENCING IN PROGRESS ***, 15 unordered pieces.    44   3e-04  3
dna update 2003.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115605_5(AC115605|pid:none) Dictyostelium discoideum chromosom...   192   2e-47
AC115605_4(AC115605|pid:none) Dictyostelium discoideum chromosom...   191   6e-47
AY596296_161(AY596296|pid:none) Haloarcula marismortui ATCC 4304...   110   1e-22
AM412317_887(AM412317|pid:none) Clostridium botulinum A str. ATC...    65   5e-09
AE015927_490(AE015927|pid:none) Clostridium tetani E88, complete...    65   5e-09
CP001393_1815(CP001393|pid:none) Anaerocellum thermophilum DSM 6...    65   6e-09
CP001393_2285(CP001393|pid:none) Anaerocellum thermophilum DSM 6...    57   1e-06
BA000016_2602(BA000016|pid:none) Clostridium perfringens str. 13...    57   1e-06
CP000885_3236(CP000885|pid:none) Clostridium phytofermentans ISD...    56   2e-06
CP001146_1364(CP001146|pid:none) Dictyoglomus thermophilum H-6-1...    55   6e-06
protein update 2009. 5.16
PSORT 

 psg: 0.48  gvh: 0.23  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for CFK216 is cyt
5' end seq. ID CFK216F
5' end seq. 
>CFK216F.Seq
AAAAGGTAAATAAATAATAAATTAAGATGTTAACATTCAAACAATTAAAAGAAACCGAAA
AAGATTTCACTGTTGATGAAAATAAATTCAGAGTTGCACCAAGCAATGAAACCTTAATGG
AAACTGTCAAGAATTTAGAAGCCAAGAACCACAGAGTTTCAGTCGTTGAAAATGAAGCTG
ATGCTTTAAATTTATTATTAAATGAAATCCCAAAAGGTTCTGAAGTCATGTGCGCTAGCA
GTTGTACCATTGATGAAATCGGTTTCAAGAAAATTTATTTCAGTGATGATTCTCCATTCG
TCAACGTTCATAAAAAGATTTTAGCTTTACCAGCTGATAAACAAGCTGATGCTCGTAAAG
ATGCTTTAACCTCACAATACTTTTTATCATCTGTAACTGCAATCTCCAAAACTGGAAATG
TTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGCGGTTTCACTGCTGCTAAAAATTTAA
TCATCGTTTCTGGTTATAACAAGATTGTTGATTCTGATGAATTAGCCNNNNNNNNNN
Length of 5' end seq. 537
3' end seq. ID CFK216Z
3' end seq. 
>CFK216Z.Seq
NNNNNNNNNNCTGTTGGCCTACTGGAAAAGGTAAATAAATAATAAATTAAGATGTTAACA
TTCAAACAATTAAAAGAAACCGAAAAAGATTTCNCTGTTGATGAAAATAAATTCAGAGTT
GCACCAAGCAATGAAACCTTAATGGAAACTGTCAAGAATTTAGAAGCCAAGAACCACAGA
GTTTCAGTCGTTGAAAATGAAGCTGATGCTTTAAATTTATTATTAAATGAAATCCCAAAA
GGTTCTGAAGTCATGTGCGCTAGCAGTTGTACCATTGATGAAATCGGTTTCAAGAAAATT
TATTTCAGTGATGATTCTCCATTCGTCAACGTTCATAAAAAGATTTTAGCTTTACCAGCT
GATAAACAAGCTGATGCTCGTAAAGATGCTTTAACCTCACAATACTTTTTATCATCTGTA
ACTGCAATCTCCAAAACTGGAAATGTTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGC
GGTTTCACTGCTGCTAAAAATTTAATCATCGTTTCTGGTTATAACAAGATTGTTGANTCT
GATGAATTAGCCGTCCAAAGAACTGAACAATTCTGTTTACCATGTGAAAGTGTTCGTGCT
CGTTGGGCTTACAAAGTTCCAGGTTCAATGATTCAAAATCNTTTGGGTATGAAACATGGT
GCTCCAAACAAACATCACATTATCCTCATNAAAAAAATA
Length of 3' end seq. 699
Connected seq. ID CFK216P
Connected seq. 
>CFK216P.Seq
AAAAGGTAAATAAATAATAAATTAAGATGTTAACATTCAAACAATTAAAAGAAACCGAAA
AAGATTTCACTGTTGATGAAAATAAATTCAGAGTTGCACCAAGCAATGAAACCTTAATGG
AAACTGTCAAGAATTTAGAAGCCAAGAACCACAGAGTTTCAGTCGTTGAAAATGAAGCTG
ATGCTTTAAATTTATTATTAAATGAAATCCCAAAAGGTTCTGAAGTCATGTGCGCTAGCA
GTTGTACCATTGATGAAATCGGTTTCAAGAAAATTTATTTCAGTGATGATTCTCCATTCG
TCAACGTTCATAAAAAGATTTTAGCTTTACCAGCTGATAAACAAGCTGATGCTCGTAAAG
ATGCTTTAACCTCACAATACTTTTTATCATCTGTAACTGCAATCTCCAAAACTGGAAATG
TTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGCGGTTTCACTGCTGCTAAAAATTTAA
TCATCGTTTCTGGTTATAACAAGATTGTTGATTCTGATGAATTAGCC----------CTG
TTGGCCTACTGGAAAAGGTAAATAAATAATAAATTAAGATGTTAACATTCAAACAATTAA
AAGAAACCGAAAAAGATTTCNCTGTTGATGAAAATAAATTCAGAGTTGCACCAAGCAATG
AAACCTTAATGGAAACTGTCAAGAATTTAGAAGCCAAGAACCACAGAGTTTCAGTCGTTG
AAAATGAAGCTGATGCTTTAAATTTATTATTAAATGAAATCCCAAAAGGTTCTGAAGTCA
TGTGCGCTAGCAGTTGTACCATTGATGAAATCGGTTTCAAGAAAATTTATTTCAGTGATG
ATTCTCCATTCGTCAACGTTCATAAAAAGATTTTAGCTTTACCAGCTGATAAACAAGCTG
ATGCTCGTAAAGATGCTTTAACCTCACAATACTTTTTATCATCTGTAACTGCAATCTCCA
AAACTGGAAATGTTTATGTTGCCGACGCTTCAGGTACCAGAGTTGGCGGTTTCACTGCTG
CTAAAAATTTAATCATCGTTTCTGGTTATAACAAGATTGTTGANTCTGATGAATTAGCCG
TCCAAAGAACTGAACAATTCTGTTTACCATGTGAAAGTGTTCGTGCTCGTTGGGCTTACA
AAGTTCCAGGTTCAATGATTCAAAATCNTTTGGGTATGAAACATGGTGCTCCAAACAAAC
ATCACATTATCCTCATNAAAAAAATA
Length of connected seq. 1216
Full length Seq ID -
Full length Seq. -
Length of full length seq. -