>AFF187 (AFF187Q) /CSM/AF/AFF1-D/AFF187Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTXXXXXX
XXXXTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAA
CCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAA
ACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGG
TCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAA
TGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGA
ATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAAT
TGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTAT
CGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGA
CTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGA
AAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGA
AAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGA
AGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATC
AACCAAAAAAGTNATCAGACGAACTTTAAATAAATAAATAA

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVI---

---SPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKP
AGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHP
LIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQE
AEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVK
KSTKKVIRRTLNK*i

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTLVI---

---SPDNTYKVLVDNKEIQAGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVKP
AGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHP
LIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQE
AEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTDDKKEEVK
KSTKKVIRRTLNK*i

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlws---

---hqitptkyssitkrskqvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmlnq
pvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyth
*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptkk
pkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklmtkkkksk
nqpkkxsdel*ink*

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihfgh---

---tr*hlqstrr*qrdpsr*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*ts
rs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpi
n**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprs
rkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n**qkrrsqk
inqksxqtnfk*in

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF187 (AFF187Q) /CSM/AF/AFF1-D/AFF187Q.Seq.d/                       2062   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       2052   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       2052   0.0
AFE410 (AFE410Q) /CSM/AF/AFE4-A/AFE410Q.Seq.d/                       2052   0.0
AFE318 (AFE318Q) /CSM/AF/AFE3-A/AFE318Q.Seq.d/                       2052   0.0
AFE168 (AFE168Q) /CSM/AF/AFE1-C/AFE168Q.Seq.d/                       2052   0.0
AFD463 (AFD463Q) /CSM/AF/AFD4-C/AFD463Q.Seq.d/                       2052   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       2038   0.0
AFG656 (AFG656Q) /CSM/AF/AFG6-C/AFG656Q.Seq.d/                       2038   0.0
AFO794 (AFO794Q) /CSM/AF/AFO7-D/AFO794Q.Seq.d/                       2026   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1142   0.0    4
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   1e-27  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   3e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80   1e-10  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74   2e-10  2
CK247437|CK247437.1 EST731074 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCAT37 5' end, mRNA sequence.    66   5e-09  3
CK253744|CK253744.1 EST737381 potato callus cDNA library, normalized and full-length Solanum tuberosum cDNA clone POCC781 5' end, mRNA sequence.    66   5e-09  3
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74   6e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74   6e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74   6e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   352   0.0
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   212   e-109
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   217   e-109
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   210   e-109
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   211   e-108
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   214   e-108
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   211   e-108
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   211   e-108
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   209   e-108
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   208   e-107

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFF187 is exc

>AFF187F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATT------
----

>AFF187Z.Seq
----------TCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGC
AGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACA
ATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACC
AGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGA
TTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAA
AGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCC
ATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGG
TGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGT
CACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGA
AGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGC
TGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGA
AGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAA
AAAATCAACCAAAAAAGTNATCAGACGAACTTTAAATAAATAAATAA

>AFF187P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATT------
----TCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAAGCAGGTAA
CCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAAACAATCCAA
ACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCGG
TCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAA
TGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGA
ATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAAT
TGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTAT
CGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGA
CTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGA
AAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGA
AAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGA
AGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGACAAAAAAGAAGAAGTCAAAAAATC
AACCAAAAAAGTNATCAGACGAACTTTAAATAAATAAATAA