>AFF179 (AFF179Q) /CSM/AF/AFF1-D/AFF179Q.Seq.d/
XXXXXXXXXXTGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAGAGA
ATATTCCACAATTCNTTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTC
ATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAG
TTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAG
CCTTGGAAGATGAATACTTTGTAAGCAGAAANCTTTATCCAAATGTTGATTTCTACTCTG
GTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTC
CACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAA
TCTATCGNCCACGTCAAGTTNACAAAGGTGAATGGTTTAGAAACTATGTCCCNATCGATG
GTAGACCACCAGCTAAAGTTCGT

---ANEAVLDMLIHIGSKENIPQFXSDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEV
FESLGKEPLIEVATELEKQALEDEYFVSRXLYPNVDFYSGLIYKAMGFPTDMFPVLFTIP
RAVGWLAHWVEHLEDPETKIYRPRQVXKGEWFRNYVPIDGRPPAKVR

Frame A:
---ANEAVLDMLIHIGSKENIPQFXSDVKSKKKKLMGFGHRIYKNYDPRAKIIRRVAYEV
FESLGKEPLIEVATELEKQALEDEYFVSRXLYPNVDFYSGLIYKAMGFPTDMFPVLFTIP
RAVGWLAHWVEHLEDPETKIYRPRQVXKGEWFRNYVPIDGRPPAKVR


Frame B:
---pmrpywic*fisvpkrifhnsfqm*rakrrn*wvsviestktmiqeprsfve*lmkf
skv*vknh*lk*plswrnkpwkmntl*aexfiqmlistlvssikqwvsqlicsqfyslfh
vplvg*lig*ni*kiqklksixhvkxtkvngletmsxsmvdhqlkf


Frame C:
---q*grigyvnsyrfqreystixfrckeqkeeingfrs*nlqkl*sksqdhs*ssl*sf
rkfr*rtid*ssh*vgetslgr*ilckqkxlskc*fllwshl*sngfpn*yvpsfihyst
crwlvsslgrtfrrsrn*nlsxtssxqr*mv*klcpxrw*tts*ss

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SSG238 (SSG238Q) /CSM/SS/SSG2-B/SSG238Q.Seq.d/                        948   0.0
SSF821 (SSF821Q) /CSM/SS/SSF8-A/SSF821Q.Seq.d/                        948   0.0
SFE393 (SFE393Q) /CSM/SF/SFE3-D/SFE393Q.Seq.d/                        948   0.0
SFC695 (SFC695Q) /CSM/SF/SFC6-D/SFC695Q.Seq.d/                        948   0.0
AFN717 (AFN717Q) /CSM/AF/AFN7-A/AFN717Q.Seq.d/                        948   0.0
AFM803 (AFM803Q) /CSM/AF/AFM8-A/AFM803Q.Seq.d/                        948   0.0
AFJ471 (AFJ471Q) /CSM/AF/AFJ4-C/AFJ471Q.Seq.d/                        948   0.0
AFI655 (AFI655Q) /CSM/AF/AFI6-C/AFI655Q.Seq.d/                        948   0.0
AFF179 (AFF179Q) /CSM/AF/AFF1-D/AFF179Q.Seq.d/                        948   0.0
AFC579 (AFC579Q) /CSM/AF/AFC5-D/AFC579Q.Seq.d/                        948   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY124377|AY124377.1 Dictyostelium discoideum citrate synthase (cshA) mRNA, complete cds.   948   0.0    1
AB109907|AB109907.1 Tetrahymena thermophila tgCS mRNA for putative citrate synthase, complete cds.   101   6e-25  3
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.    76   5e-21  2
AF310892|AF310892.1 Dictyostelium discoideum citrate synthase (gltA) gene, partial cds; RacF2 (racF2) gene, complete cds; and unknown gene.    76   5e-21  2
AL385088|AL385088.1 Medicago truncatula EST MtBC26C06F1 : T3 end of clone MtBC26C06 of  cDNA library MtBC from arbuscular mycorrhiza of cultivar Jemalong of Medicago truncatula (barrel medic).    98   8e-20  2
BU044708|BU044708.1 PP_LEa0020E04f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0020E04f, mRNA sequence.    64   2e-11  2
BU040616|BU040616.1 PP_LEa0006L02f Peach developing fruit mesocarp Prunus persica cDNA clone PP_LEa0006L02f, mRNA sequence.    64   3e-11  2
CB821747|CB821747.1 EST 2598 Half-Ripe Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone be003m23 3', mRNA sequence.    64   2e-09  2
BU010710|BU010710.1 QGJ14E07.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGJ14E07, mRNA sequence.    58   5e-09  2
BQ987715|BQ987715.1 QGF12P22.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGF12P22, mRNA sequence.    58   5e-09  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK120755_1(AK120755|pid:none) Oryza sativa Japonica Group cDNA c...   234   6e-61
AB109907_1(AB109907|pid:none) Tetrahymena thermophila tgCS mRNA ...   231   5e-60
AF310892_1(AF310892|pid:none) Dictyostelium discoideum citrate s...   230   1e-59
AB167269_1(AB167269|pid:none) Chlorobium limicola cit gene for c...   227   1e-58
CP001099_363(CP001099|pid:none) Chlorobaculum parvum NCIB 8327, ...   227   1e-58
(P49299) RecName: Full=Citrate synthase, glyoxysomal;          E...   224   7e-58
CP001110_2246(CP001110|pid:none) Pelodictyon phaeoclathratiforme...   222   3e-57
CP000096_332(CP000096|pid:none) Pelodictyon luteolum DSM 273, co...   222   4e-57
AB334779_1(AB334779|pid:none) Glycine max mRNA for peroxisomal c...   221   1e-56
CR954217_232(CR954217|pid:none) Ostreococcus tauri strain OTTH05...   220   1e-56

 psg: 0.75  gvh: 0.30  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: cytoskeletal
	 28.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for AFF179 is cyt

>AFF179Z.Seq
----------TGCCAATGAGGCCGTATTGGATATGTTAATTCATATCGGTTCCAAAGAGA
ATATTCCACAATTCNTTTCAGATGTAAAGAGCAAAAAGAAGAAATTAATGGGTTTCGGTC
ATAGAATCTACAAAAACTATGATCCAAGAGCCAAGATCATTCGTAGAGTAGCTTATGAAG
TTTTCGAAAGTTTAGGTAAAGAACCATTGATTGAAGTAGCCACTGAGTTGGAGAAACAAG
CCTTGGAAGATGAATACTTTGTAAGCAGAAANCTTTATCCAAATGTTGATTTCTACTCTG
GTCTCATCTATAAAGCAATGGGTTTCCCAACTGATATGTTCCCAGTTTTATTCACTATTC
CACGTGCCGTTGGTTGGTTAGCTCATTGGGTAGAACATTTAGAAGATCCAGAAACTAAAA
TCTATCGNCCACGTCAAGTTNACAAAGGTGAATGGTTTAGAAACTATGTCCCNATCGATG
GTAGACCACCAGCTAAAGTTCGT