>AFF177 (AFF177Q) /CSM/AF/AFF1-D/AFF177Q.Seq.d/
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGNCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GTXXXXXXXXXXTCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCA
ACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATT
AGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATT
ACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCG
TCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGA
ACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAA
GAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGC
CGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAAC
TTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAA
ATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAAC
AACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTC
GACTAGATTTATTACAGCCCTTTGCTATCAATTTTT

ftfrsyilihn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAG
---

---LEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELL
KQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKK
TLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKL
GLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFITALCYQF

Frame A:
tshlgpif*fiinficffl*nikkwlnhklnvshsklklislcplllilfiqikkyf*en
*fqmhlmp*ikfvtnh*lmhqfwnqklnsklklfqirlpkllhslivvlv*pkpiw*ri*
vlllvqvlktlwnnykvvplisv*lxnlvlvstllt*lpilllftlktmmmnntfgnqvl
---

---s*shlhv*pn**iccstikri*r*eisinyqrrfeir*nrr*keks*tr*scq*rit
qts*rcig**s*kgsslnsscqltmcfsyl*iwlvskhgtyhessssp*lfnviihvike
nfriksrspnr*rfsqksrrkk*nfqrlcllii*ncslnfrflir*tiiicftyssyd*i
rfkhsr*fisnn*rinkhnnq**ystirrkr*ti*kekvd*iyyspllsif

Frame B:
lhi*vlysns*lilfvffcrt*kng*its*tfhips*n*sayvpyy*yflfk*rsifkri
dfkci*clr*nslpiin*cisfgikn*trn*nysr*dcqnsyth**wywydqnrygkefr
yycsfry*klygtitkwcr*yqydwxiwcwfllcllscrycycsl*kq***tirlgikcw
---

---LEVIYMCDPIDEYAVQQLKEYDGKKLVSITKEGLKLDETEDEKKKAEQDKAANEELL
KQVKDVLGDKVEKVVLSTRLANSPCVLVTSEYGWSANMERIMKAQALRDSSMSSYMSSKK
TLELNPDHPIVRDLAKKAAEKSKTFKDFVYLLYETALLTSGFSLDEPSSFASRIHRMIKL
GLSIQDDSSATTEESTNTTTSDDIPPLEENDEPSERKKSTRFITALCYQF

Frame C:
ftfrsyilihn*FYLFFFVEHKKMAESQVERFTFQAEINQLMSLIINTFYSNKEVFLREL
ISNASDALDKIRYQSLTDASVLESKTELEIKIIPDKTAKTLTLIDSGIGMTKTDMVKNLG
TIARSGTKNFMEQLQSGAADISMIGQFGVGFYSAYLVADTVIVHSKNNDDEQYVWESSAG
---

---lksftcvtqlmnmlfnn*knmtvrn*yqlpkkv*n*mkpkmkrkklnkiklpmknys
nklkmywviklkr*fsqlvlpthhvf*lplnmvgqqtwnvs*klklsvtlqchhtchqrk
l*n*iqitqslei*pkkppkkvklsktlftyymklls*lqvsh*mnhhhllhvfiv*ln*
v*afkmihqqqlknqqtqqpvmifhh*kktmnhlkgksrldllqpfainf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ442 (VHQ442Q) /CSM/VH/VHQ4-B/VHQ442Q.Seq.d/                        973   0.0
VHP152 (VHP152Q) /CSM/VH/VHP1-C/VHP152Q.Seq.d/                        973   0.0
VHM703 (VHM703Q) /CSM/VH/VHM7-A/VHM703Q.Seq.d/                        973   0.0
VHK340 (VHK340Q) /CSM/VH/VHK3-B/VHK340Q.Seq.d/                        973   0.0
VHK280 (VHK280Q) /CSM/VH/VHK2-D/VHK280Q.Seq.d/                        973   0.0
VHP202 (VHP202Q) /CSM/VH/VHP2-A/VHP202Q.Seq.d/                        963   0.0
VHL676 (VHL676Q) /CSM/VH/VHL6-D/VHL676Q.Seq.d/                        963   0.0
VHL421 (VHL421Q) /CSM/VH/VHL4-A/VHL421Q.Seq.d/                        963   0.0
VHK487 (VHK487Q) /CSM/VH/VHK4-D/VHK487Q.Seq.d/                        963   0.0
VHJ821 (VHJ821Q) /CSM/VH/VHJ8-A/VHJ821Q.Seq.d/                        963   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

L43591|L43591.1 Dictyostelium discoideum (clone C 6.1.1) heat shock cognate protein (hsc90) mRNA, complete cds.   926   0.0    3
L34027|L34027.1 Plasmodium falciparum (clone Dd2) heat shock protein 86 gene, complete cds.    92   2e-52  9
L34028|L34028.1 Plasmodium falciparum (clone HB3) heat shock protein 86 gene, complete cds.    92   3e-52  8
Z29667|Z29667.1 P.falciparum (7) mRNA for heat-shock protein.      92   4e-50  8
AF165818|AF165818.4 Guillardia theta nucleomorph chromosome 1, complete sequence.    80   1e-47  13
AF030694|AF030694.2 Plasmodium falciparum strain Dd2 heat shock protein 86 (HSP86), O1 (o1), O3 (o3), O2 (o2), CG8 (cg8), CG4 (cg4), CG3 (cg3), putative chloroquine resistance transporter (crt), CG9 (cg9), CG1 (cg1), CG6 (cg6), CG2 (cg2), and CG7 (cg7) genes, complete cds.    92   5e-46  10
AY122634|AY122634.1 Naegleria gruberi strain ATCC 30224 heat shock protein 90 (HSP-90) gene, partial cds.    82   4e-45  7
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           92   1e-41  25
AF249998|AF249998.1 Habrotrocha constricta 82 kDa heat shock protein 2 (hsp82-2) gene, partial cds.    76   4e-41  4
AY423866|AY423866.1 Cryptosporidium parvum heat shock protein 90 gene, partial cds.    68   5e-41  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54651) RecName: Full=Heat shock cognate 90 kDa protein;             303   8e-81
L43591_1(L43591|pid:none) Dictyostelium discoideum (clone C 6.1....   303   8e-81
AB300196_1(AB300196|pid:none) Physarum polycephalum hsp90 mRNA f...   251   3e-65
EF687776_1(EF687776|pid:none) Crassostrea gigas heat shock prote...   244   4e-63
EF532406_1(EF532406|pid:none) Argopecten irradians heat shock pr...   239   1e-61
AF006542_1(AF006542|pid:none) Drosophila pseudoobscura bogotana ...   238   2e-61
AF006561_1(AF006561|pid:none) Drosophila miranda strain miranda2...   238   2e-61
AF006543_1(AF006543|pid:none) Drosophila pseudoobscura bogotana ...   238   2e-61
(O16087) RecName: Full=Heat shock protein 83; AltName: Full=HSP ...   238   2e-61
C24827(C24827) heat shock 82K protein - fruit fly (Drosophila ps...   238   2e-61

 psg: 0.50  gvh: 0.46  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFF177 is nuc

>AFF177F.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGNCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GT----------

>AFF177Z.Seq
----------TCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCAAC
AATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATTAG
ATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATTAC
TCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCGTC
TTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGAAC
GTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAAGA
AAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGCCG
AAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAACTT
CAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAAAT
TAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAACAA
CCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTCGA
CTAGATTTATTACAGCCCTTTGCTATCAATTTTT

>AFF177P.Seq
ACTTCACATTTAGGTCCTATATTCTAATTCATAATTAATTTTATTTGTTTTTTTTTGTAG
AACATAAAAAAATGGCTGAATCACAAGTTGAACGTTTCACATTCCAAGCTGAAATTAATC
AGCTTATGTCCCTTATTATTAATACTTTTTATTCAAATAAAGAAGTATTTTTAAGAGAAT
TGATTTCAAATGCATCTGATGCCTTAGATAAAATTCGTTACCAATCATTAACTGATGCAT
CAGTTTTGGAATCAAAAACTGAACTCGAAATTAAAATTATTCCAGATAAGACTGCCAAAA
CTCTTACACTCATTGATAGTGGTATTGGTATGACCAAAACCGATATGGTAAAGAATTTAG
GTACTATTGCTCGTTCAGGTACTAAAAACTTTATGGAACAATTACAAAGTGGTGCCGCTG
ATATCAGTATGATTGGNCAATTTGGTGTTGGTTTCTACTCTGCTTACTTAGTTGCCGATA
CTGTTATTGTTCACTCTAAAAACAATGATGATGAACAATACGTTTGGGAATCAAGTGCTG
GT----------TCTTGAAGTCATTTACATGTGTGACCCAATTGATGAATATGCTGTTCA
ACAATTAAAAGAATATGACGGTAAGAAATTAGTATCAATTACCAAAGAAGGTTTGAAATT
AGATGAAACCGAAGATGAAAAGAAAAAAGCTGAACAAGATAAAGCTGCCAATGAAGAATT
ACTCAAACAAGTTAAAGATGTATTGGGTGATAAAGTTGAAAAGGTAGTTCTCTCAACTCG
TCTTGCCAACTCACCATGTGTTTTAGTTACCTCTGAATATGGTTGGTCAGCAAACATGGA
ACGTATCATGAAAGCTCAAGCTCTCCGTGACTCTTCAATGTCATCATACATGTCATCAAA
GAAAACTTTAGAATTAAATCCAGATCACCCAATCGTTAGAGATTTAGCCAAAAAAGCCGC
CGAAAAAAGTAAAACTTTCAAAGACTTTGTTTACTTATTATATGAAACTGCTCTCTTAAC
TTCAGGTTTCTCATTAGATGAACCATCATCATTTGCTTCACGTATTCATCGTATGATTAA
ATTAGGTTTAAGCATTCAAGATGATTCATCAGCAACAACTGAAGAATCAACAAACACAAC
AACCAGTGATGATATTCCACCATTAGAAGAAAACGATGAACCATCTGAAAGGAAAAAGTC
GACTAGATTTATTACAGCCCTTTGCTATCAATTTTT