NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF150
Library AF
(Link to library)
Clone ID AFF150
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13825-1
Original site URL
Representative seq. ID AFF150Z
(Link to Original site)
Representative DNA sequence 
>AFF150 (AFF150Q) /CSM/AF/AFF1-C/AFF150Q.Seq.d/
XXXXXXXXXXTCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGT
TGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCG
TCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGG
TCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGA
TGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGA
ACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACAC
TCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCA
ATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGT
CGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATAT
TATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGC
CTCATGTGAAGATATTGCAAGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTGTTAA
AGAAGCTGCTAGGATGCTTACGATAAGCCAATCCATATG
sequence update 2001. 6. 2
Translated Amino Acid sequence 
---VVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPTDKAGR
VEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPSIIYTH
PEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVLGAHIM
GTNAGELIGECVLAMEYGASCEDIARTCHGHPTLSEAVKEAARMLTISQSI
Translated Amino Acid sequence (All Frames) 
Frame A:
---lslknlmvrll*plnklvlvvslvlsklmlslsllvvvqilvvlvlnqlvsqpiklv
vskleitltlkfhqfsqlvmpsvvqclltklkkkvllslnkftmvvvmltmvpshplstl
tqkllglvrlkknykkkvsntilvdshslqtlepkqtmmskvslnssplkilieslvlil
wvpmlvn*lvnvf*lwnmvphvkilqelvmviqlypkllkkllgclr*anpy


Frame B:
---cr*ki*w*gycnr*tswcwwfhwysrs*ccpclcwsssky*wsws*iswypnr*sws
crswrsl*h*ssinfrnw*chpwsnacsqs*rrrycyh*tnsqwwwsc*lwchpihylhs
prsclgw*d*rritkrrypiqyw*ipirckl*sqnkr*crrfr*iprr*rf**spwcsyy
gyqcw*inw*mcfsygiwclm*rycknlswssnfirsc*rsc*daydkpihm


Frame C:
---VVKKSDGKVTVTVEQVGAGGFTGTLEADAVLVSVGRRPNTSGLGLESVGIPTDKAGR
VEVGDHFNTKVPSIFAIGDAIRGPMLAHKAEEEGIAIIEQIHNGGGHVNYGAIPSIIYTH
PEVAWVGKTEEELQKEGIQYNIGRFPFVANSRAKTNDDVEGFVKFLAAKDSDRVLGAHIM
GTNAGELIGECVLAMEYGASCEDIARTCHGHPTLSEAVKEAARMLTISQSI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFD715 (VFD715Q) /CSM/VF/VFD7-A/VFD715Q.Seq.d/                       1366   0.0
VFC622 (VFC622Q) /CSM/VF/VFC6-A/VFC622Q.Seq.d/                       1366   0.0
AFF150 (AFF150Q) /CSM/AF/AFF1-C/AFF150Q.Seq.d/                       1366   0.0
AFE149 (AFE149Q) /CSM/AF/AFE1-C/AFE149Q.Seq.d/                       1366   0.0
AFD276 (AFD276Q) /CSM/AF/AFD2-D/AFD276Q.Seq.d/                       1366   0.0
AFC230 (AFC230Q) /CSM/AF/AFC2-B/AFC230Q.Seq.d/                       1366   0.0
AFB294 (AFB294Q) /CSM/AF/AFB2-D/AFB294Q.Seq.d/                       1362   0.0
SFF721 (SFF721Q) /CSM/SF/SFF7-A/SFF721Q.Seq.d/                       1358   0.0
VFH845 (VFH845Q) /CSM/VF/VFH8-B/VFH845Q.Seq.d/                       1356   0.0
VFE339 (VFE339Q) /CSM/VF/VFE3-B/VFE339Q.Seq.d/                       1334   0.0
own update 2001.11.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AY466389|AY466389.1 Dictyostelium discoideum dihydrolipoamide dehydrogenase (Lpd) gene, partial cds.   589   e-164  1
AL423544|AL423544.1 T3 end of clone AZ0AA009B01 of library AZ0AA from strain CBS 712 of Kluyveromyces marxianus.    84   4e-25  3
BZ296408|BZ296408.1 CG2037.f11 Candida glabrata Random Genomic Library Candida glabrata genomic clone CG2037, DNA sequence.    64   1e-24  3
AL428740|AL428740.1 clone BA0AB027E08 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    70   7e-21  3
AX489387|AX489387.1 Sequence 6687 from Patent WO02053728.          62   2e-20  3
AR180935|AR180935.1 Sequence 7 from patent US 6333164.             62   5e-18  3
AL439032|AL439032.1 T3 end of clone BD0AA002A06 of library BD0AA from strain CBS 94 of  Candida tropicalis.    54   1e-17  4
BJ599701|BJ599701.1 Physcomitrella patens subsp. patens cDNA clone:pphn27c08, 3' end, single read.    50   3e-15  5
BJ588943|BJ588943.1 Physcomitrella patens subsp. patens cDNA clone:pphb37e23, 3' end, single read.    50   6e-15  5
BJ603296|BJ603296.1 Physcomitrella patens subsp. patens cDNA clone:pphn1e18, 3' end, single read.    50   1e-14  5
dna update 2004. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391421_1(AY391421|pid:none) Danio rerio dihydrolipoamide dehyd...   260   3e-68
AF542528_19(AF542528|pid:none) Cryptococcus neoformans var. grub...   259   4e-68
AY710429_12(AY710429|pid:none) Cryptococcus gattii strain E566 M...   259   5e-68
AE017344_576(AE017344|pid:none) Cryptococcus neoformans var. neo...   259   7e-68
Z82277_3(Z82277|pid:none) Caenorhabditis elegans Cosmid LLC1, co...   258   1e-67
AP007171_417(AP007171|pid:none) Aspergillus oryzae RIB40 genomic...   258   2e-67
AY710430_14(AY710430|pid:none) Cryptococcus gattii strain WM276 ...   258   2e-67
BT045027_1(BT045027|pid:none) Salmo salar clone ssal-rgf-510-070...   257   2e-67
AM270136_18(AM270136|pid:none) Aspergillus niger contig An07c019...   254   2e-66
CR954211_302(CR954211|pid:none) Ostreococcus tauri strain OTTH05...   253   4e-66
protein update 2009. 4.19
PSORT 

 psg: 0.76  gvh: 0.43  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFF150 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFF150Z
3' end seq. 
>AFF150Z.Seq
----------TCTGTCGTTAAAAAATCTGATGGTAAGGTTACTGTAACCGTTGAACAAGT
TGGTGCTGGTGGTTTCACTGGTACTCTCGAAGCTGATGCTGTCCTTGTCTCTGTTGGTCG
TCGTCCAAATACTAGTGGTCTTGGTCTTGAATCAGTTGGTATCCCAACCGATAAAGCTGG
TCGTGTCGAAGTTGGAGATCACTTTAACACTAAAGTTCCATCAATTTTCGCAATTGGTGA
TGCCATCCGTGGTCCAATGCTTGCTCACAAAGCTGAAGAAGAAGGTATTGCTATCATTGA
ACAAATTCACAATGGTGGTGGTCATGTTAACTATGGTGCCATCCCATCCATTATCTACAC
TCACCCAGAAGTTGCTTGGGTTGGTAAGACTGAAGAAGAATTACAAAAAGAAGGTATCCA
ATACAATATTGGTAGATTCCCATTCGTTGCAAACTCTAGAGCCAAAACAAACGATGATGT
CGAAGGTTTCGTTAAATTCCTCGCCGCTAAAGATTCTGATAGAGTCCTTGGTGCTCATAT
TATGGGTACCAATGCTGGTGAATTAATTGGTGAATGTGTTTTAGCTATGGAATATGGTGC
CTCATGTGAAGATATTGCAAGAACTTGTCATGGTCATCCAACTTTATCCGAAGCTGTTAA
AGAAGCTGCTAGGATGCTTACGATAAGCCAATCCATATG
Length of 3' end seq. 689
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -