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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFF147
Library AF
(Link to library)
Clone ID AFF147
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15511-1
Original site URL
Representative seq. ID AFF147P
(Link to Original site)
Representative DNA sequence 
>AFF147 (AFF147Q) /CSM/AF/AFF1-B/AFF147Q.Seq.d/
ATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAACAACA
ACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAAAAAAA
XXXXXXXXXXAGATGATCAATCAATTTGTATGTGGGATATTTCAGCAGCATCAAAATCAG
ATTCCACTTTGGATGCATTAAACATTTACAATGGTCATACATCAATTGTTGAAGATGTAG
CATGGCATTATATTCACGATACATTTCTTGGATCAGTAGGCGATGATAAGAAACTTATGA
TTTGGGATACTCGTACTGGAACAAAACCAATTCACGTTGTTGAAGCACATAATAGCGAAG
TCAATTGTTTATCATTCAATCCATTTTGTGAGTTTTTAGTTGCAACTGGTTCAACCGATA
AGACCGTTGCTCTTTGGGATATGCGTAATTTAGGTAATCGTCTTCATTCTTTAATCTCTC
ATACTGATGAAGTGTTTCAAGTTCAATTTTCACCACATAATGAAACGGTTTTGGCAAGTT
GTGGTTCTGATCGTCGTGTAAACGTTTGGGATCTCTCTAGAATAGGTGAAGAACAAAACA
ATGAAGAT
sequence update 2001. 6. 2
Translated Amino Acid sequence 
inkiyayiqivl*iiikttttkii*q*sfiffy*YKLKKK---

---DDQSICMWDISAASKSDSTLDALNIYNGHTSIVEDVAWHYIHDTFLGSVGDDKKLMI
WDTRTGTKPIHVVEAHNSEVNCLSFNPFCEFLVATGSTDKTVALWDMRNLGNRLHSLISH
TDEVFQVQFSPHNETVLASCGSDRRVNVWDLSRIGEEQNNED
Translated Amino Acid sequence (All Frames) 
Frame A:
inkiyayiqivl*iiikttttkii*q*sfiffy*YKLKKK---

---r*sinlyvgyfssikirfhfgcikhlqwsyinc*rcsmalysryiswisrr**etyd
lgysywnktnsrc*st**rsqlfiiqsil*vfscnwfnr*drcslgya*fr*sssffnls
y**svsssiftt**ngfgklwf*ssckrlgsl*nr*rtkq*r

Frame B:
*ikymhifk*fyk***kqqqqk*ynnnllfffinin*kk---

---DDQSICMWDISAASKSDSTLDALNIYNGHTSIVEDVAWHYIHDTFLGSVGDDKKLMI
WDTRTGTKPIHVVEAHNSEVNCLSFNPFCEFLVATGSTDKTVALWDMRNLGNRLHSLISH
TDEVFQVQFSPHNETVLASCGSDRRVNVWDLSRIGEEQNNED

Frame C:
k*niciysnsfinnnknnnnknnitiifyfflli*ikkk---

---minqfvcgifqqhqnqiplwmh*tftmvihqllkm*hgiiftihfldq*amirnl*f
gilvleqnqftllkhiiaksivyhsihfvsf*lqlvqpirpllfgicvi*vivfil*sli
lmkcfkfnfhhimkrfwqvvvlivv*tfgisle*vknktmk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFF147 (AFF147Q) /CSM/AF/AFF1-B/AFF147Q.Seq.d/                        948   0.0
AFL441 (AFL441Q) /CSM/AF/AFL4-B/AFL441Q.Seq.d/                        934   0.0
AFI439 (AFI439Q) /CSM/AF/AFI4-B/AFI439Q.Seq.d/                        928   0.0
VFJ175 (VFJ175Q) /CSM/VF/VFJ1-D/VFJ175Q.Seq.d/                        924   0.0
AFL229 (AFL229Q) /CSM/AF/AFL2-B/AFL229Q.Seq.d/                        862   0.0
VFD507 (VFD507Q) /CSM/VF/VFD5-A/VFD507Q.Seq.d/                        704   0.0
AFA262 (AFA262Q) /CSM/AF/AFA2-C/AFA262Q.Seq.d/                        573   e-162
AFD468 (AFD468Q) /CSM/AF/AFD4-C/AFD468Q.Seq.d/                        432   e-120
AFD891 (AFD891Q) /CSM/AF/AFD8-D/AFD891Q.Seq.d/                         86   7e-16
SFL265 (SFL265Q) /CSM/SF/SFL2-C/SFL265Q.Seq.d/                         72   1e-11
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

CD079902|CD079902.1 MA3-9999U-M280-B08-U.B MA3-0001 Schistosoma mansoni cDNA cloneMA3-9999U-M280-B08.B, mRNA sequence.    52   5e-16  4
CD074099|CD074099.1 MA3-0001U-M280-B08-U.G MA3-0001 Schistosoma mansoni cDNA clone MA3-0001U-M280-B08.G, mRNA sequence.    46   5e-12  3
BU794126|BU794126.1 SJF2CCC03 SJF Schistosoma japonicum cDNA, mRNA sequence.    50   8e-11  4
BI862961|BI862961.1 rm14f05.y1 Meloidogyne arenaria egg pAMP1 v1 Chiapelli McCarter Meloidogyne arenaria cDNA 5' similar to SW:RB48_HUMAN Q09028 CHROMATIN ASSEMBLY FACTOR 1 P48 SUBUNIT ;, mRNA sequence.    50   9e-10  3
BC003092|BC003092.1 Homo sapiens, Similar to retinoblastoma binding protein 4, clone MGC:1393, mRNA, complete cds.    58   5e-06  2
BE779693|BE779693.1 601465216F1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE:3868659 5', mRNA sequence.    60   4e-05  1
BF980929|BF980929.1 602304475F1 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:4395666 5', mRNA sequence.    58   2e-04  1
AW854058|AW854058.1 RC3-CT0254-230300-028-h09 CT0254 Homo sapiens cDNA, mRNA sequence.    58   2e-04  1
CQ731204|CQ731204.1 Sequence 17138 from Patent WO02068579.         58   2e-04  1
CD620898|CD620898.1 56052454H1 FLP Homo sapiens cDNA, mRNA sequence.    58   2e-04  1
dna update 2004. 5. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SD4) RecName: Full=Probable histone-binding protein rbbD;         333   2e-90
(Q24572) RecName: Full=Probable histone-binding protein Caf1; Al...   235   8e-61
BC077257_1(BC077257|pid:none) Xenopus laevis retinoblastoma A as...   233   2e-60
(Q9W7I5) RecName: Full=Histone-binding protein RBBP4; AltName: F...   233   2e-60
AK299251_1(AK299251|pid:none) Homo sapiens cDNA FLJ53908 complet...   233   2e-60
AK222779_1(AK222779|pid:none) Homo sapiens mRNA for retinoblasto...   233   2e-60
(O93377) RecName: Full=Histone-binding protein RBBP4-A; AltName:...   233   2e-60
(Q09028) RecName: Full=Histone-binding protein RBBP4; AltName: F...   233   2e-60
(Q5M7K4) RecName: Full=Histone-binding protein RBBP4; AltName: F...   233   2e-60
(Q6INH0) RecName: Full=Histone-binding protein RBBP4-B; AltName:...   233   2e-60
protein update 2009. 4.19
PSORT 

 psg: 0.76  gvh: 0.40  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial

>> prediction for AFF147 is cyt
5' end seq. ID AFF147F
5' end seq. 
>AFF147F.Seq
ATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAACAACA
ACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAAAAAAA
----------
Length of 5' end seq. 120
3' end seq. ID AFF147Z
3' end seq. 
>AFF147Z.Seq
----------AGATGATCAATCAATTTGTATGTGGGATATTTCAGCAGCATCAAAATCAG
ATTCCACTTTGGATGCATTAAACATTTACAATGGTCATACATCAATTGTTGAAGATGTAG
CATGGCATTATATTCACGATACATTTCTTGGATCAGTAGGCGATGATAAGAAACTTATGA
TTTGGGATACTCGTACTGGAACAAAACCAATTCACGTTGTTGAAGCACATAATAGCGAAG
TCAATTGTTTATCATTCAATCCATTTTGTGAGTTTTTAGTTGCAACTGGTTCAACCGATA
AGACCGTTGCTCTTTGGGATATGCGTAATTTAGGTAATCGTCTTCATTCTTTAATCTCTC
ATACTGATGAAGTGTTTCAAGTTCAATTTTCACCACATAATGAAACGGTTTTGGCAAGTT
GTGGTTCTGATCGTCGTGTAAACGTTTGGGATCTCTCTAGAATAGGTGAAGAACAAAACA
ATGAAGAT
Length of 3' end seq. 478
Connected seq. ID AFF147P
Connected seq. 
>AFF147P.Seq
ATAAATAAAATATATGCATATATTCAAATAGTTTTATAAATAATAATAAAAACAACAACA
ACAAAAATAATATAACAATAATCTTTTATTTTTTTTTATTAATATAAATTAAAAAAAAAA
----------AGATGATCAATCAATTTGTATGTGGGATATTTCAGCAGCATCAAAATCAG
ATTCCACTTTGGATGCATTAAACATTTACAATGGTCATACATCAATTGTTGAAGATGTAG
CATGGCATTATATTCACGATACATTTCTTGGATCAGTAGGCGATGATAAGAAACTTATGA
TTTGGGATACTCGTACTGGAACAAAACCAATTCACGTTGTTGAAGCACATAATAGCGAAG
TCAATTGTTTATCATTCAATCCATTTTGTGAGTTTTTAGTTGCAACTGGTTCAACCGATA
AGACCGTTGCTCTTTGGGATATGCGTAATTTAGGTAATCGTCTTCATTCTTTAATCTCTC
ATACTGATGAAGTGTTTCAAGTTCAATTTTCACCACATAATGAAACGGTTTTGGCAAGTT
GTGGTTCTGATCGTCGTGTAAACGTTTGGGATCTCTCTAGAATAGGTGAAGAACAAAACA
ATGAAGAT
Length of connected seq. 598
Full length Seq ID -
Full length Seq. -
Length of full length seq. -